fa طراحی کاندید واکسن چند اپی‌توپی علیه تب کیو بر پایه پروتئین‌های غشای خارجی OMP1 و LolB : یک مطالعه ایمونوانفورماتیک میکروب‌شناسی و ایمنولوژی Microbiology and Immunology پژوهشي Original <div style="text-align: justify;"><span style="font-size:12pt"><span style="line-height:110%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:10.0pt"><span style="line-height:110%"><span iransans="" style="font-family:"><span style="color:#1f4e79">زمینه:</span></span></span></span></b> <span lang="FA" style="font-size:10.0pt"><span style="line-height:110%"><span iransans="" style="font-family:"><span style="color:#0d0d0d">تب کیو (کوکسیلا بورنتی)، بیماری مشترک بین انسان و حیوان است و می‌تواند مشکلات جدی مانند نارسایی‌های کبدی، قلبی و عروقی، پنومونی و خستگی‌های مزمن طولانی را در میزبان ایجاد کند. با توجه به تأثیر مهم کوکسیلا بورنتی بر سلامت جامعه و محدودیت در استفاده از واکسن‌های طراحی شده قبلی، هدف از این مطالعه طراحی واکسن تب کیو بر اساس پروتئین‌های غشای خارجی کوکسیلا بورنتی با استفاده از روش‌های بیوانفورماتیک بوده است.</span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:110%"><span iransans="" style="font-family:"><span style="color:#0d0d0d"></span></span></span></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="line-height:110%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:10.0pt"><span style="line-height:110%"><span iransans="" style="font-family:"><span style="color:#1f4e79">مواد و روش‌ها:</span></span></span></span></b><b> </b><span lang="FA" style="font-size:10.0pt"><span style="line-height:110%"><span iransans="" style="font-family:"><span style="color:#0d0d0d"><span style="letter-spacing:-.1pt">اپی‌توپ‌های تحریک کننده لنفوسیت‌های </span></span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:110%"><span iransans="" style="font-family:"><span style="color:#0d0d0d"><span style="letter-spacing:-.1pt">B</span></span></span></span></span><span lang="FA" style="font-size:10.0pt"><span style="line-height:110%"><span iransans="" style="font-family:"><span style="color:#0d0d0d"><span style="letter-spacing:-.1pt"> و </span></span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:110%"><span iransans="" style="font-family:"><span style="color:#0d0d0d"><span style="letter-spacing:-.1pt">T</span></span></span></span></span><span lang="FA" style="font-size:10.0pt"><span style="line-height:110%"><span iransans="" style="font-family:"><span style="color:#0d0d0d"><span style="letter-spacing:-.1pt"> پروتئین‌های </span></span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:110%"><span iransans="" style="font-family:"><span style="color:#0d0d0d"><span style="letter-spacing:-.1pt">OMP1</span></span></span></span></span><span lang="FA" style="font-size:10.0pt"><span style="line-height:110%"><span iransans="" style="font-family:"><span style="color:#0d0d0d"><span style="letter-spacing:-.1pt"> و </span></span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:110%"><span iransans="" style="font-family:"><span style="color:#0d0d0d"><span style="letter-spacing:-.1pt">LolB</span></span></span></span></span><span lang="FA" style="font-size:10.0pt"><span style="line-height:110%"><span iransans="" style="font-family:"><span style="color:#0d0d0d"><span style="letter-spacing:-.1pt"> با استفاده از سرورهای </span></span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:110%"><span iransans="" style="font-family:"><span style="color:#0d0d0d"><span style="letter-spacing:-.1pt">IEDB</span></span></span></span></span><span lang="FA" style="font-size:10.0pt"><span style="line-height:110%"><span iransans="" style="font-family:"><span style="color:#0d0d0d"><span style="letter-spacing:-.1pt"> و </span></span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:110%"><span iransans="" style="font-family:"><span style="color:#0d0d0d"><span style="letter-spacing:-.1pt">ABCpred</span></span></span></span></span><span lang="FA" style="font-size:10.0pt"><span style="line-height:110%"><span iransans="" style="font-family:"><span style="color:#0d0d0d"><span style="letter-spacing:-.1pt"> شناسایی شد. پس از ارزیابی ویژگی‌های مختلف مانند عدم سمیت (سرور </span></span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:110%"><span iransans="" style="font-family:"><span style="color:#0d0d0d"><span style="letter-spacing:-.1pt">ToxinPred</span></span></span></span></span><span lang="FA" style="font-size:10.0pt"><span style="line-height:110%"><span iransans="" style="font-family:"><span style="color:#0d0d0d"><span style="letter-spacing:-.1pt">)، عدم ایجاد آلرژی (سرور </span></span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:110%"><span iransans="" style="font-family:"><span style="color:#0d0d0d"><span style="letter-spacing:-.1pt">AllerTOP</span></span></span></span></span><span lang="FA" style="font-size:10.0pt"><span style="line-height:110%"><span iransans="" style="font-family:"><span style="color:#0d0d0d"><span style="letter-spacing:-.1pt">) و توانایی تحریک سیستم ایمنی (سرور </span></span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:110%"><span iransans="" style="font-family:"><span style="color:#0d0d0d"><span style="letter-spacing:-.1pt">VaxiJen</span></span></span></span></span><span lang="FA" style="font-size:10.0pt"><span style="line-height:110%"><span iransans="" style="font-family:"><span style="color:#0d0d0d"><span style="letter-spacing:-.1pt">)، اپی‌توپ‌های نهایی انتخاب و کاندید واکسن نوترکیب طراحی شد. ویژگیهای کاندید واکسن طراحی شده با استفاده از سرورهای مختلف (</span></span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:110%"><span iransans="" style="font-family:"><span style="color:#0d0d0d"><span style="letter-spacing:-.1pt">ProtParam</span></span></span></span></span><span lang="FA" style="font-size:10.0pt"><span style="line-height:110%"><span iransans="" style="font-family:"><span style="color:#0d0d0d"><span style="letter-spacing:-.1pt">، </span></span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:110%"><span iransans="" style="font-family:"><span style="color:#0d0d0d"><span style="letter-spacing:-.1pt">Solpro</span></span></span></span></span><span lang="FA" style="font-size:10.0pt"><span style="line-height:110%"><span iransans="" style="font-family:"><span style="color:#0d0d0d"><span style="letter-spacing:-.1pt">،</span></span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:110%"><span iransans="" style="font-family:"><span style="color:#0d0d0d"><span style="letter-spacing:-.1pt">PSIPRED </span></span></span></span></span><span lang="FA" style="font-size:10.0pt"><span style="line-height:110%"><span iransans="" style="font-family:"><span style="color:#0d0d0d"><span style="letter-spacing:-.1pt">، </span></span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:110%"><span iransans="" style="font-family:"><span style="color:#0d0d0d"><span style="letter-spacing:-.1pt">Cluspro</span></span></span></span></span><span lang="FA" style="font-size:10.0pt"><span style="line-height:110%"><span iransans="" style="font-family:"><span style="color:#0d0d0d"><span style="letter-spacing:-.1pt"> و </span></span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:110%"><span iransans="" style="font-family:"><span style="color:#0d0d0d"><span style="letter-spacing:-.1pt">C-ImmSim</span></span></span></span></span><span lang="FA" style="font-size:10.0pt"><span style="line-height:110%"><span iransans="" style="font-family:"><span style="color:#0d0d0d"><span style="letter-spacing:-.1pt"> ) ارزیابی شد. </span></span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:110%"><span iransans="" style="font-family:"><span style="color:#0d0d0d"><span style="letter-spacing:-.1pt"></span></span></span></span></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="line-height:110%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:10.0pt"><span style="line-height:110%"><span iransans="" style="font-family:"><span style="color:#1f4e79"><span style="letter-spacing:-.2pt">یافته‌ها:</span></span></span></span></span></b> <span lang="FA" style="font-size:10.0pt"><span style="line-height:110%"><span iransans="" style="font-family:"><span style="color:#0d0d0d"><span style="letter-spacing:-.2pt">در نهایت، برای پروتئین‌ </span></span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:110%"><span iransans="" style="font-family:"><span style="color:#0d0d0d"><span style="letter-spacing:-.2pt">OMP1</span></span></span></span></span><span lang="FA" style="font-size:10.0pt"><span style="line-height:110%"><span iransans="" style="font-family:"><span style="color:#0d0d0d"><span style="letter-spacing:-.2pt">، 3 ‌اپی‌توپ تحریک کننده لنفوسیت </span></span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:110%"><span iransans="" style="font-family:"><span style="color:#0d0d0d"><span style="letter-spacing:-.2pt">B</span></span></span></span></span><span lang="FA" style="font-size:10.0pt"><span style="line-height:110%"><span iransans="" style="font-family:"><span style="color:#0d0d0d"><span style="letter-spacing:-.2pt"> و 17 اپی‌توپ تحریک کننده لنفوسیت </span></span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:110%"><span iransans="" style="font-family:"><span style="color:#0d0d0d"><span style="letter-spacing:-.2pt">T</span></span></span></span></span><span lang="FA" style="font-size:10.0pt"><span style="line-height:110%"><span iransans="" style="font-family:"><span style="color:#0d0d0d"><span style="letter-spacing:-.2pt"> شناسایی شد. همچنین 1 اپی‌توپ تحریک کننده لنفوسیت </span></span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:110%"><span iransans="" style="font-family:"><span style="color:#0d0d0d"><span style="letter-spacing:-.2pt">B</span></span></span></span></span><span lang="FA" style="font-size:10.0pt"><span style="line-height:110%"><span iransans="" style="font-family:"><span style="color:#0d0d0d"><span style="letter-spacing:-.2pt"> و 9 اپی‌توپ تحریک کننده لنفوسیت </span></span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:110%"><span iransans="" style="font-family:"><span style="color:#0d0d0d"><span style="letter-spacing:-.2pt">T</span></span></span></span></span><span lang="FA" style="font-size:10.0pt"><span style="line-height:110%"><span iransans="" style="font-family:"><span style="color:#0d0d0d"><span style="letter-spacing:-.2pt"> برای پروتئین‌ </span></span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:110%"><span iransans="" style="font-family:"><span style="color:#0d0d0d"><span style="letter-spacing:-.2pt">LolB</span></span></span></span></span><span lang="FA" style="font-size:10.0pt"><span style="line-height:110%"><span iransans="" style="font-family:"><span style="color:#0d0d0d"><span style="letter-spacing:-.2pt"> شناسایی شد. ویژگی‌های مختلف کاندید واکسن طراحی شده مانند ویژگی‌های فیزیکوشیمیایی، ساختار ثانویه، عدم سمیت و توانایی تحریک سیستم ایمنی مثبت پیش بینی شد.</span></span></span></span></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="line-height:110%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:10.0pt"><span style="line-height:110%"><span iransans="" style="font-family:"><span style="color:#1f4e79"><span style="letter-spacing:-.2pt">نتیجه‌گیری:</span></span></span></span></span></b> <span lang="FA" style="font-size:10.0pt"><span style="line-height:110%"><span iransans="" style="font-family:"><span style="color:#0d0d0d"><span style="letter-spacing:-.2pt">بر اساس نتایج محاسباتی مطالعه حاضر، کاندید واکسن طراحی شده ممکن است به خوبی سیستم ایمنی را تحریک و موجب ایمنی در برابر بیماری تب کیو شود. جهت اطمینان از اثربخشی و ایمن بودن کاندید واکسن طراحی شده مطالعات تکمیلی در شرایط آزمایشگاهی و بدن موجود زنده ضروری است.</span></span></span></span></span></span></span></span></span></span></div> <div style="text-align: justify;"><span style="font-size:12pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:9.0pt"><span style="line-height:115%"><span iransans="" style="font-family:"><span style="color:#002060">Background: </span></span></span></span></b><span style="font-size:9.0pt"><span style="line-height:115%"><span iransans="" style="font-family:"><span style="color:#404040">Q fever&mdash;caused by Coxiella burnetii&mdash;is a zoonotic disease reported worldwide. It can cause severe complications in the host, manifesting with conditions such as liver, heart, and vascular failure, pneumonia, and long-term chronic fatigue. Given the significant impact of C. burnetii on public health and the limitations of previously designed vaccines, this study aimed to design a Q fever vaccine based on C. burnetii outer membrane proteins using bioinformatics methods.</span></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:9.0pt"><span style="line-height:115%"><span iransans="" style="font-family:"><span style="color:#002060">Materials and Methods:</span></span></span></span></b><span style="font-size:9.0pt"><span style="line-height:115%"><span iransans="" style="font-family:"><span style="color:#404040"> Epitopes that stimulate B- and T-lymphocytes were identified using the ABCpred and IEDB servers. After evaluating various properties&mdash;such as non-toxicity (ToxinPred server), lack of allergenicity (AllerTOP server), and immunogenicity (VaxiJen server)&mdash;a recombinant vaccine was designed. The characteristics of the vaccine candidate were evaluated using different servers (ProtParam, Solpro, PSIPRED, Cluspro, and C-ImmSim). </span></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:9.0pt"><span style="line-height:115%"><span iransans="" style="font-family:"><span style="color:#002060">Results: </span></span></span></span></b><span style="font-size:9.0pt"><span style="line-height:115%"><span iransans="" style="font-family:"><span style="color:#404040">Following comprehensive evaluations, three B-lymphocyte-stimulating epitopes and 17 T-lymphocyte-stimulating epitopes were selected for the OMP1 and LolB proteins, respectively. In addition, 17 and 9 T-lymphocyte-stimulating epitopes were identified for OMP1 and LolB, respectively. Various properties of the designed vaccine candidate, including physicochemical properties, secondary structure, expression levels, non-toxicity, and ability to elicit a positive immune system, were predicted.</span></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:9.0pt"><span style="line-height:115%"><span iransans="" style="font-family:"><span style="color:#002060"><span style="letter-spacing:.2pt">Conclusion:</span></span></span></span></span></b><span style="font-size:9.0pt"><span style="line-height:115%"><span iransans="" style="font-family:"><span style="color:#404040"><span style="letter-spacing:.2pt"> Based on the computational findings of this study, the designed vaccine may effectively stimulate the immune system and provide immunity against Q fever. Further in vitro and in vivo studies are necessary to ensure the effectiveness and safety of the designed vaccine.</span></span></span></span></span></span></span></span></div> تب کیو, کوکسیلا بورنتی, واکسن, OMP1, LolB Q fever, Coxiella burnetiid, Vaccine, OMP1, LolB 123 139 http://ismj.bpums.ac.ir/browse.php?a_code=A-10-1115-93&slc_lang=fa&sid=1 Hamid Vaez حمید واعظ h_vaez@zbmu.ac.ir 5700319475328460020599 5700319475328460020599 Yes Department of Microbiology, School of Medicine, Zabol University of Medical Sciences, Zabol, Iran <br>Pediatric Gastroenterology and Hepatology Research Center, Zabol University of Medical Sciences, Zabol, Iran گروه میکروب‌شناسی، دانشکده پزشکی، دانشگاه علوم پزشکی زابل، زابل، ایران<br>مرکز تحقیقات گوارش و کبد کودکان، دانشگاه علوم پزشکی زابل، زابل، ایران Ladan Shahrakimojahed لادن شهرکی مجاهد 5700319475328460020600 5700319475328460020600 No Veterinary organization, Sistan and Baluchistan, Zabol, Iran سازمان دامپزشکی، اداره دامپزشکی سیستان و بلوچستان، زابل، ایران