Volume 20, Issue 5 (Iranian South Medical Journal 2017)
Iran South Med J 2017, 20(5): 426-436 |
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Moghadam A, Nazarian S. Molecular Typing Isolates of Salmonella Enterica Serovar Infantis Using Eric-PCR Method. Iran South Med J 2017; 20 (5) :426-436
URL: http://ismj.bpums.ac.ir/article-1-896-en.html
URL: http://ismj.bpums.ac.ir/article-1-896-en.html
1- Department of Cell and Molecular Science, School of Biology, University of tehran, Tehran, Iran
2- Department of Biology, School of Science, Imam Hossain University, Tehran, Iran , kpnazari@ihu.ac.ir
2- Department of Biology, School of Science, Imam Hossain University, Tehran, Iran , kpnazari@ihu.ac.ir
Abstract: (4484 Views)
Background: Salmonella are significant bacteria of the Enterobacteriaceae which are very diverse biochemically and serologically. These bacteria are primarily transmitted through food ingestion. The spread of non-typhoid Salmonella is one of the challenging issues in the current medical research. Therefore, rapid diagnosis and identifying the type of these pathogens provide crucial information for early detection and controlling the spread of the infection. The aim of this study was typing the clinical strains of Salmonella Infantis.
Materials and Methods: In this study, strains of Salmonella Infantis were isolated from several health centers. All of the strains were identified by standard microbiology, biochemical and molecular methods. Genetic relationship between strains was analyzed using the ERIC-PCR method.
Results: In this study, 842 stool and blood sample of patients with diarrhea were examined, and 48 different strains linked to Salmonella Infantis were isolated. Strains categorized into 14 different groups by genotyping using the ERIC-PCR method, and the highest number of the strains were placed in group 5 (20%, 10 strains).
Conclusion: Results of this study revealed that strains of Salmonella Infantis which were examined genetically were rather diverse in nature. This could be due to the high prevalence of polyclonal strains in human samples. It was also shown that ERIC-PCR method has an abundant differential power for the molecular typing purposes.
Materials and Methods: In this study, strains of Salmonella Infantis were isolated from several health centers. All of the strains were identified by standard microbiology, biochemical and molecular methods. Genetic relationship between strains was analyzed using the ERIC-PCR method.
Results: In this study, 842 stool and blood sample of patients with diarrhea were examined, and 48 different strains linked to Salmonella Infantis were isolated. Strains categorized into 14 different groups by genotyping using the ERIC-PCR method, and the highest number of the strains were placed in group 5 (20%, 10 strains).
Conclusion: Results of this study revealed that strains of Salmonella Infantis which were examined genetically were rather diverse in nature. This could be due to the high prevalence of polyclonal strains in human samples. It was also shown that ERIC-PCR method has an abundant differential power for the molecular typing purposes.
Type of Study: Original |
Subject:
Microbiology and Immunology
Received: 2017/02/1 | Accepted: 2017/04/6 | Published: 2017/11/13
Received: 2017/02/1 | Accepted: 2017/04/6 | Published: 2017/11/13
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