1- Immunology Department, Tehran University of Medical Science, Tehran, Iran 2- Biochemistry Department, Yazd University of Medical Science, Yazd, Iran 3- Biochemistry Department, Yazd University of Medical Science, Yazd, Iran , firstname.lastname@example.org 4- Student Research Center, Shiraz University of Medical Science, Shiraz, Iran
Abstract: (1792 Views)
Background: Curcumin and quercetinare two natural substances with low side effects and has antioxidant activity, anti-diabetic and strong anti-cancer and other health benefits. The aim of this study was the evaluation of these two compounds as anti-cancer agents and their toxicity on murine 4T1 breast cancer cells by MTT method.
Materials and Methods: In this experimental study, after cells culturing in 96-well plate 5, 10, 20, 30, 40 micromolar concentrations of curcumin and quercetin were added to the cells and after incubation during 24 and 48 hours, cell viability were evaluated by MTT method. one way-Anova was used to analysis data. p<0.05 was considered as significant level.
Results: The result of this study showed that IC50 at 24 hours for curcumin was 14.8±4 micromolar and for quercetin 21.7±0.7 micromolar per ml and for 48 hours for curcumin was 21±0.3 micromolar and for quercetin 18.2±0.45 micromolar.
Conclusion: In this study we showed that cell survival were depended on curcumin and quercetin concentration and time of incubation. By increasing the concentration of solution, toxicity have increased and cell survival have decrease at 48 hours more than 24 hours.
Type of Study: Original |
Subject: Pharmacology Received: 2017/02/26 | Accepted: 2017/02/26 | Published: 2017/02/26
1. Forrest AP, Stewart HJ, Everington D, et al. Randomised controlled trial of conservation therapy for breast cancer: 6-year analysis of the Scottish trial. Lancet 1996; 348(9029): 708-13. [PubMed] [Google Scholar]
2. Moreillon JJ, Bowden RG, Deike E, et al. The use of an anti-inflammatory supplement in patients with chronic kidney disease. J Complement Integr Med 2013; 10(1): 143-52. [PubMed] [Google Scholar]
3. Steigerwalt R, Nebbioso M, Appendino G, et al. Meriva®, a lecithinized curcumin delivery system, in diabetic microangiopathy and retinopathy. Panminerva Med 2012; 54(40): 11-6. [PubMed] [Google Scholar]
4. Chandran B, Goel A. A randomized, pilot study to assess the efficacy and safety of curcumin in patients with active rheumatoid arthritis. Phytother Res 2012; 26(11): 1719-25. [PubMed] [Google Scholar]
5. Baum L, Lam CW, Cheung SK, et al. Six-month randomized, placebo-controlled, double-blind, pilot clinical trial of curcumin in patients with Alzheimer disease. J Clin Psychopharmacol 2008; 28(1): 110-3. [PubMed] [Google Scholar]
6. Anand P, Sundaram C, Jhurani S, et al. Curcumin and cancer: an “old-age” disease with an “age-old” solution. Cancer lett 2008; 267(1): 133-64. [PubMed] [Google Scholar]
7. Calabrese V, Bates TE, Mancuso C, et al. Curcumin and the cellular stress response in free radical‐related diseases. Mol Nutr Food Res 2008; 52(9): 1062-73. [PubMed] [Google Scholar]
8. Sharma RA, Gescher AJ, Steward WP. Curcumin: the story so far. Eur J Cancer 2005; 41(13): 1955-68. [PubMed] [Google Scholar]
9. Bimonte, S, Barbieri A, Palma G, et al. Curcumin inhibits tumor growth and angiogenesis in an orthotopic mouse model of human pancreatic cancer. Biomed Res Int 2013; 2013: 810423. [PubMed] [Google Scholar]
10. Zhang M, Swarts SG, Yin L, et al. Antioxidant properties of quercetin. Oxygen Transport to Tissue XXXII. USA: Springer, 2011, 283-9. [PubMed] [Google Scholar]
11. Comalada M, Camuesco D, Sierra S, et al. In vivo quercitrin anti‐inflammatory effect involves release of quercetin, which inhibits inflammation through down‐regulation of the NF‐κB pathway. Eur J Immunol 2005; 35(2): 584-92. [PubMed] [Google Scholar]
12. Passioti M, Maggina P, Megremis S, et al. The common cold: potential for future prevention or cure. Curr Allergy Asthma Rep 2014; 14(2): 413. [PubMed] [Google Scholar]
13. Gulati N, Laudet B, Zohrabian VM, et al. The antiproliferative effect of Quercetin in cancer cells is mediated via inhibition of the PI3K-Akt/PKB pathway. Anticancer Res 2006; 269(2A): 1177-81. [PubMed] [Google Scholar]
14. Deng XH, Song HY, Zhou YF, et al. Effects of quercetin on the proliferation of breast cancer cells and expression of survivin in vitro. Exp Ther Med 2013; 6(5): 1155-8. [PubMed] [Google Scholar]
15. Sun M, Nie S, Pan X, et al. Quercetin-nanostructured lipid carriers: characteristics and anti-breast cancer activities in vitro. Colloids Surf B Biointerfaces 2014; 113: 15-24. [PubMed] [Google Scholar]
16. Kumar SR, Priyatharshni S, Babu VN, et al. Quercetin conjugated superparamagnetic magnetite nanoparticles for in-vitro analysis of breast cancer cell lines for chemotherapy applications. J Colloid Interface Sci 2014; 436: 234-42. [PubMed] [Google Scholar]
17. Oh SJ, Kim O, Lee JS, et al. Inhibition of angiogenesis by quercetin in tamoxifen-resistant breast cancer cells. Food Chem Toxicol 2010; 48(11): 3227-34. [PubMed] [Google Scholar]
18. Kunwar A, Barik A, Mishra B, et al. Quantitative cellular uptake, localization and cytotoxicity of curcumin in normal and tumor cells. Biochim Biophys Acta 2008; 1780(4): 673-9. [PubMed] [Google Scholar]
19. Mendonça LM, Dos Santos GC, Antonucci GA, et al. Evaluation of the cytotoxicity and genotoxicity of curcumin in PC12 cells. Mutat Res 2009; 675(1): 29-34. [PubMed] [Google Scholar]
20. Friedman L, Lin L, Ball S, et al. Curcumin analogues exhibit enhanced growth suppressive activity in human pancreatic cancer cells. Anticancer Drugs 2009; 20(6): 444-9. [PubMed] [Google Scholar]
21. Liu TY, Tan ZJ, Jiang L, et al. Curcumin induces apoptosis in gallbladder carcinoma cell line GBC-SD cells. Cancer Cell Int 2013; 13(1): 64. [PubMed] [Google Scholar]
22. Zhang F, Cui Y, Cao P. Effect of quercetin on proliferation and apoptosis of human nasopharyngeal carcinoma HEN1 cells. J Huazhong Univ Sci Technolog Med Sci 2008; 28 (3): 369-72. [PubMed] [Google Scholar]
23. Vidya Priyadarsini R, Senthil Murugan R, Maitreyi S, et al. The flavonoid quercetin induces cell cycle arrest and mitochondria-mediated apoptosis in human cervical cancer (HeLa) cells through p53 induction and NF-κB inhibition. Eur J Pharmacol 2010; 649(1): 84-91. [PubMed] [Google Scholar]
24. Singhal RL, Yeh YA, Prajda N, et al. Quercetin down-regulates signal transduction in human breast carcinoma cells. Biochem Biophys Res Commun 1995; 208(1): 425-31. [PubMed] [Google Scholar]
Mansourabadi A, Hematti M, Moradi A, Maghsoudi A. Evaluation of Curcumin and Quercetin Toxicity Effects on 4T1 Murine Breast Cancer Cell Line by MTT Method . Iran South Med J. 2017; 20 (1) :1-8 URL: http://ismj.bpums.ac.ir/article-1-852-en.html