:: Volume 22, Issue 6 (Iranian South Medical Journal 2020) ::
Iran South Med J 2020, 22(6): 392-401 Back to browse issues page
The Effect of Probiotic Bifidobacterium Lactis and Lactobacillus Casei on Sperm Maturation in Streptozotocin-Diabetic Rats
Samira Abasi1 , Zahra Keshtmand 2
1- Department of Biolog, School of Science, Central Tehran Branch Islamic Azad University, Tehran, Iran
2- Department of Biolog, School of Science, Central Tehran Branch Islamic Azad University, Tehran, Iran , zkeshtmand2001@gmail.com
Abstract:   (774 Views)
Background: Diabetes is one of the most prevalent diseases in the world with its side effects, for instance in reproductive system. Probiotics are beneficial microorganisms that have preventive and therapeutic effects. The present study aimed to evaluate the effect of probiotics Bifidobacterium lactis and Lactobacillus casei on sperm maturation in streptozotocin-induced diabetic rats.
Materials and Methods: In this experimental study, 35 male Wistar rats were divided into five groups: control, diabetic (type 1), diabetic rats treated with B.lactis and L.casei and a mixture of both probiotics. Diabetic groups were injected intraperitoneally with streptozotocin (60mg/kg). Probiotics were administered for 35 days. At the end of treatment, blood glucose levels, epididymal weight and sperm maturation were evaluated. The percentage of histone-protamine replacement was evaluated by aniline blue staining.
Results: In the present study, blood glucose level in the diabetic group was significantly increased compared to the control group (P<0.001), however, the diabetic groups treated with Lactobacillus casei and Bifidobacterium lactis showed a significant decrease compared to the diabetic group (P<0.001).
The percentage of immature sperms was significantly increased in diabetic rats compared to the control group (P<0.001), and there was a significant increase in probiotic treatment groups compared with diabetic group (P<0.05).
Conclusion: Probiotics B. lactis and L. casei have a positive effect on lowering blood glucose and improving sperm maturation in diabetic rats.
Keywords: Streptozotocin, Diabetic, Lactobacillus casei, Bifidobacterium lactis, Rat
Full-Text [PDF 678 kb]   (161 Downloads)    
Type of Study: Original | Subject: Microbiology and Immunology
Received: 2019/07/1 | Accepted: 2019/10/22 | Published: 2020/01/28
1. Kautzky-Willer A, Harreiter J, Winhofer-Stöckl Y, et al. Gestational Diabetes Mellitus (Update 2019). Wien Klin Wochenschr 2019; 131(1): 91-102. [DOI:10.1007/s00508-018-1419-8]
2. Wandell PE. Quality of Life of Patients with Diabetes Mellitus, An Overview of Research in Primary Health Care in the Nordic Countries. Scand J Prim Health Care 2005; 23(2): 68-74. [DOI:10.1080/02813430510015296]
3. Tangvarasittichai S.Oxidative Stress, Insulin Resistance, Dyslipidemia and Type 2 Diabetes Mellitus. World J Diabetes 2015; 6(3): 456-80. [DOI:10.4239/wjd.v6.i3.456]
4. Al-Numair KS, Chandramohan G, Veeramani C, et al. Ameliorative Effect of Kaempferol, a Flavonoid, on Oxidative Stress in Streptozotocin-Induced Diabetic Rats. Redox Rep 2015; 20(5): 198-209. [DOI:10.1179/1351000214Y.0000000117]
5. Aboonabi A, Rahmat A, Othman F. Antioxidant Effect of Pomegranate Against Streptozotocin-Nicotinamide Generated Oxidative Stress Induced Diabetic Rats. Toxicol Rep 2014; 1: 915-22. [DOI:10.1016/j.toxrep.2014.10.022]
6. Kianifard D, Sadrkhanlou RJ, Hasanzadeh SH. The Ultrastructural Changes of the Sertoli and Leydig Cells Following Streptozotocin Induced Diabetes. Iran J Basic Med Sci 2012; 15(1): 623-35.
7. Ahn C, An BS, Jeung EB. Streptozotocin Induces Endoplasmic Reticulum Stress and Apoptosis Via Disruption of Calcium Homeostasis in Mouse Pancreas. Mol Cell Endocrinol 2015; 412: 302-8. [DOI:10.1016/j.mce.2015.05.017]
8. Omolaoye S, Temidayo S, Du Plessis S. Diabetes Mellitus and Male Infertility. Asian Pac J Reprod 2018; 7(1): 6-14. [DOI:10.4103/2305-0500.220978]
9. Abbasi Z, Fatemi Tabatabaei SR, Mazaheri Y, et al. Effects of Sesame Oil on the Reproductive Parameters of Diabetes Mellitus-Induced Male Rats. World J Mens Health 2013; 31(2): 141-9. [DOI:10.5534/wjmh.2013.31.2.141]
10. Szkudelski T. The Mechanism of Alloxan and Streptozotocin Action in B Cells of the Rat Pancreas. Physiol Res 2001; 50(6): 536-46.
11. Morimoto S, Mendoza-Rodriguez CA, Hiriart M, et al. Protective Effect of Testosterone on Early Apoptotic Damage Induced by Streptozotocin in Rat Pancreas. J Endocrinol 2005; 187(2): 217-24. [DOI:10.1677/joe.1.06357]
12. Korejo NA, Wei QW, Shah AH, et al. Effects of Concomitant Diabetes Mellitus and Hyperthyroidism on Testicular and Epididymal Histoarchitecture and Steroidogenesis in Male Animals. J Zhejiang Univ Sci B 2016; 17(11): 850-63. [DOI:10.1631/jzus.B1600136]
13. Baccetti B, La Marca A, Piomboni P, et al. Insulin-Dependent Diabetes in Men is Associated with Hypothalamo-Pituitary Derangement and with Impairment in Semen Quality. Hum Reprod 2002; 17(10): 2673-7. [DOI:10.1093/humrep/17.10.2673]
14. Halliwell B. Free Radicals and Antioxidants: Updating A Personal View. Nutr Rev 2012; 70(5): 257-65. [DOI:10.1111/j.1753-4887.2012.00476.x]
15. Dos Santos JM, Tewari S, Mendes RH. The Role of Oxidative Stress in the Development of Diabetes Mellitus and Its Complications. J Diabetes Res 2019; 1-3. [DOI:10.1155/2019/4189813]
16. Asmat U, Abad K, Ismail K. Diabetes Mellitus and Oxidative Stress-A Concise Review. Saudi Pharm J 2016; 24(5): 547-53. [DOI:10.1016/j.jsps.2015.03.013]
17. Zhan CN, Li FX, Xu WN, et al. Combined Effects of Dietary Fructooligosac Charide and Bacillus Licheniform on Innate Immunity Antioxidant Capability and Disease Resistance of Triangular Bream(Megalobram Terminalis). Fish Shellfish Immunol 2013; 35(5): 1380-6. [DOI:10.1016/j.fsi.2013.07.047]
18. Imani Fooladi AA, Yazdi MH, Pourmand MR, et al. Th1 Cytokine Production Induced by Lactobacillus acidophilus in BALB/c Mice Bearing Transplanted Breast Tumor. Jundishapur J Microbiol 2015; 8(4): e17354. [DOI:10.5812/jjm.8(4)2015.17354]
19. De Andrade Calaça PR, Bezerra RP, Albuquerque WWC, et al. Probiotics as a Preventive Strategy for Surgical Infection in Colorectal Cancer Patients: A Systematic Review and Meta-Analysis of Randomized Trials. Transl Gastroenterol Hepatol 2017; 2(8): 67. [DOI:10.21037/tgh.2017.08.01]
20. Mohammadi J, Mirzaei A, Azizi A, et al. The effects of hydroalcoholic extract of Juglans regia leaf on histological changes of Langerhans islet in diabetic rats model. Iran South Med J 2012; 15(4): 293-302.
21. Mazloom Z, Yousefinejad A, Dabbaghmanesh MH. Effect of Probiotics on Lipid Profile, Glycemic Control, Insulin Action, Oxidative Stress, and Inflammatory Markers in Patients with Type 2 Diabetes: a Clinical Trial. Iran J Med Sci 2013; 38(1): 38-43.
22. Shikhbahaei F, Ghanbari A, Keshtmand Z. Therapeutic Effect of Thymoquinone Against MethotrexateInduced Damage on Sperm Parameters in Mice. Int J Morphol 2018; 36(2): 519-22. [DOI:10.4067/S0717-95022018000200519]
23. Wong A, Chuan SS, Patton WC, et al. Addition of Eosin to the Aniline Blue Assay to Enhance Detection of Immature Sperm Histones. Fertil Steril 2008; 90(5): 1999-2002. [DOI:10.1016/j.fertnstert.2007.09.026]
24. Yadav H, Jain S, Sinha PR. Antidiabetic Effect of Probiotic Dahi Containing Lactobacillus Acidophilus and Lactobacillus Casei in High Fructose Fed Rats. Nutrition 2007; 23(1): 62-8. [DOI:10.1016/j.nut.2006.09.002]
25. Yadav H, Jain S, Sinha PR. Oral Administration of Dahi Containing Probiotic Lactobacillus Acidophilus and Lactobacillus Casei Delayed the Progression of Streptozotocin-Induced Diabetes in Rats. J Dairy Res 2008; 75(2): 189-95. [DOI:10.1017/S0022029908003129]
26. Badkoyeh H, Jafari P, Akbarei N. The Effect of Lactobacillus Casei Native Iranian TD2 Strain on Blood Glucose in Male Wistar Diabetic Rats. J Anim Physiol Dev 2013; 6(3): 17-22. (Persian)
27. Kumawat M, Sharma TK, Singh I, et al. Antioxidant Enzymes and Lipid Peroxidation in Type 2 Diabetes Mellitus Patients with and without Nephropathy. N Am J Med Sci 2013; 5(3): 213-9. [DOI:10.4103/1947-2714.109193]
28. Shaikh H, Shrivastava VK, Amir M. Diabetes Mellitus and Impairment of Male Reproductive Function: Role of Hypothalamus Pituitary Testicular Axis and Reactive Oxygen Species. Iran J Diabetes Obes 2016; 8(1): 41-50.
29. Komljenovic D, Sandhoff R, Tiegler A, et al. Disruption of Blood-Testis Barrier Dynamics in Ether-Lipid-Deficient Mice. Cell Tissue Res 2009; 337(2): 281-99. [DOI:10.1007/s00441-009-0809-7]

XML   Persian Abstract   Print

Volume 22, Issue 6 (Iranian South Medical Journal 2020) Back to browse issues page