:: Volume 12, Issue 2 (Iranian South Medical of Journal 2009) ::
Iran South Med J 2009, 12(2): 111-118 Back to browse issues page
Evaluation of HP0605 and HP0971 genes of efflux pumps in Helicobacter pylori resistance to Metronidazole
Mohammad hasan Shirazi Mr 1, Mohammad Mehdi Soltan dalal Mr, Sara Haji khani Ms, Mohammad reza Pourmand Mr2, Jalil Fallah Mehrabadi Mr, Naser Moghimi Dariaie Mr, Mehrndokht Sirous Ms2, Seied Saeed Eshraghi Mr
1- , mhshirazi@sina.tums.ac.ir
2- Health School
Abstract:   (14822 Views)
Background: The presence of antibiotic resistance has been reported in H.pylori and it is a major cause of treatment failure. Five families of multidrug efflux pumps are defined in bacteria and resistance-nodulation-division (RND) pumps are found mainly in gram negative bacteria. TolC is one of RND pump components and play a critical role in drug resistance. It hasn’t been established that RND family has a role in drug resistance in H.pylori. In this study, we assessed the role of two efflux genes in resistant to metronidazole in H.pylori by evaluation of overexpression TolC genes by RT-PCR method. Methods: In five metronidazole resistant strains of H.pylori, total RNA was extracted. RNA treated with DNase and RNA reverse transcribed to cDNA. Aliquots of the cDNA solution were assayed by RT- PCR for HP0605 and HP0971 genes. The levels of mRNA expression were evaluated by densitometry analysis. Results: All five strains displayed overexpression for HP0605 basis of increased concentration of metronidazole. Three strains showed transcripts for HP0971. One of these had transcripts for HP0971 only in Metronidazole concentration equaled to 16 µg/ml but two strains overexpressed adapt to increase concentration of metronidazole. Conclusion: According to current study, HP0605 and HP0971 genes overexpressed due to increase metronidazole. So, increasing of Metronidazole affects in H.pylori΄s efflux system in transcription level.
Keywords: H.pylori, efflux pump, TolC, Metronidazole
Full-Text [PDF 311 kb]   (1735 Downloads)    
Type of Study: Original | Subject: General
Received: 2009/12/12 | Accepted: 2009/12/12 | Published: 2009/12/12

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Volume 12, Issue 2 (Iranian South Medical of Journal 2009) Back to browse issues page