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:: Volume 14, Issue 4 (Iranian South Medical Journal 2011) ::
Iran South Med J 2011, 14(4): 220-229 Back to browse issues page
Study of P53 gene mutations in promoter and exons 2-4 and 9-11 in patient with gastric cancer by PCR-SSCP in Chaharmahal Va Bakhtiari province
Javad Saffari Chaleshtary 1, Mohammad taghi Moradi 1, Effat Farrokhi 1, Mohammad Amin Tabatabaiefar 2, Maryam Taherzadeh 1, Fatemeh Azadegan 1, Gholamreza Mobini 1, Mehdi Banitalebi 1, Ghorban Ali Rahimian 3, Morteza Hashemzadeh Chaleshtary * 4
1- Cellular and Molecular Research Center, ShahreKord University of Medical Sciences, Shahrekord, IRAN
2- Tehran University of Medical Sciences, Tehran, IRAN
3- School of Medicine, ShahreKord University of Medical Sciences, Shahrekord, IRAN
4- Cellular and Molecular Research Center, ShahreKord University of Medical Sciences, Shahrekord, IRAN , mchalesh@yahoo.com
Abstract:   (10507 Views)

Background: Gastric cancer is one of the most important diseases and after lung cancer, is the second cause of cancer death worldwide. Genetic factors including oncogenes and tumor suppressor genes are always contributed in progression of this cancer. The P53 tumor suppressor gene has a broad role in genomic stability and DNA repair. The aim of this study was to determine the P53 gene mutations in gastric cancer specimens in Chaharmahal Va Bakhtiari Province. Methods: In this descriptive-lab based study, we investigated the promoter, exons 2-4 and 9-11 of P53 gene mutations in 38 paraffin embedded gastric cancer specimens. DNA was extracted following the standard phenol chloroform protocol. The P53 gene mutations were determined using PCR-SSCP procedure. Results: Our study revealed no P53 gene mutation in promoter and exons 2-4 and 9-11 in the gastric cancer subjects studied. Conclusion: While P53 gene mutations have been reported as the most frequent genetic alterations and are found in about 50% of the human malignancies, no mutation was detected in this study. The reason may be due to small sample size or mutations on other genes or epigenetic factors.

Keywords: gastric cancer, mutation, P53 gene, promoter, PCR- SSCP
Full-Text [PDF 500 kb]   (1446 Downloads)    
Type of Study: Original | Subject: General
Received: 2011/11/30 | Accepted: 2011/11/30 | Published: 2011/11/30
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Saffari Chaleshtary J, Moradi M T, Farrokhi E, Tabatabaiefar M A, taherzadeh M, Azadegan F, et al . Study of P53 gene mutations in promoter and exons 2-4 and 9-11 in patient with gastric cancer by PCR-SSCP in Chaharmahal Va Bakhtiari province. Iran South Med J. 2011; 14 (4) :220-229
URL: http://ismj.bpums.ac.ir/article-1-300-en.html


Volume 14, Issue 4 (Iranian South Medical Journal 2011) Back to browse issues page
دانشگاه علوم پزشکی بوشهر، طب جنوب ISMJ

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