:: Volume 15, Issue 3 (Iranian South Medical Journal 2012) ::
Iran South Med J 2012, 15(3): 171-182 Back to browse issues page
The effect of 8 weeks of endurance training on hypothalamic Nesfatin-1 gene expression and its concentration in male rats
Abbas Ghanbari Niaki 1, Fatemeh Hosseinpour2, Rozita Fathi2, Alireza Safai-Kenari2
1- Faculty of Physical Education & Sport Sciences, University of Mazandaran, Mazandaran, IRAN , ghanbara@umz.ac.ir
2- Faculty of Physical Education & Sport Sciences, University of Mazandaran, Mazandaran, IRAN
Abstract:   (11034 Views)

Background: Hypothalamus is mentioned as the major center of appetite and energy balance. Physical activity and the exersice are able to disturb the energy balance to negative. Nesfatin-1 is a regulating neuropeptide that is produced by hypothalamus and has an important role in establishing energy balance. The purpose of this study was to examine the effect of endurance training regimen on nesfatin-1 gene expression and its concentration in the male rat hypothalamus. Materials and Methods: Eleven adult wistar male rats (8-10 week old, 130-145g) assigned into control(C, n=5) and training (E, n=6) groups. Training group was given exercise on a motor-driven treadmill (20m/min, 0% grade, 60 min/session, 5days/week for 8 weeks). Rats were sacrificed 72h after the last training session and then the hypothalamus tissue was excised for determination of nesfatin-1 gene expression and its concentration by RT-PCR & ELIZA methods, respectively. Four hours before the experiment the food not tap water was removed from the animal cages. Data was analyzed by using an independent t-student test. Results: The current results indicated that the levels of nesfatin-1 gene expression and its concentration, ATP, and glycogen concentrations were non-significantly lower in trained group when compared with control group. Conclusion: This research showed for the first time, that a low-intensity exercises, decreases nesfatin-1 expression and concentration in the hypothalamus, which accompanied insignificant reduction in energy source. It seems that in the present research, the exercise has had the same fasting and being hungry like effect on nesfatin-1 expression and concentration in the hypothalamus.

Keywords: Nesfatin-1, Nucleobindin-2, anorexigenic protein, gene expression, male rat
Full-Text [PDF 416 kb]   (2271 Downloads)    
Type of Study: Original | Subject: General
Received: 2011/01/11 | Accepted: 2011/05/12 | Published: 2012/06/17


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Volume 15, Issue 3 (Iranian South Medical Journal 2012) Back to browse issues page