:: Volume 16, Issue 5 (Iranian South Medical Journal 2013) ::
Iran South Med J 2013, 16(5): 288-295 Back to browse issues page
Diagnosis of Neisseria gonorrhoeae among pregnant women by culture method and PCR on cppB gene
Jalal Mardaneh, Parvin Hasanzadeh 1, Mohammad Motamedifar, Khadijeh Ahmadi, Farhad Nikkhahi
1- , hassanzadeh@susc.ac.ir
Abstract:   (8421 Views)

Background: Neisseria gonorrhoeae is a human obligate pathogen and the etiological agent of gonorrhea. Health irreparable complications resulting from gonorrhea disease occur mainly in pregnant women and neonates. Aim of this study was diagnosis of Neisseria gonorrhoeae among pregnant women with using culture and molecular method by amplification of cppB gene with PCR. Material and Methods: In this cross-sectional study, two endocervical swab specimens were obtained from 1100 pregnant women who referred to Shiraz Hospitals. Culture on nonselective and selective media and nucleic acid amplification test (NAAT) were performed for detection of Neisseria gonorrhoeae cppB gene. Results: All endocervical swabs cultures on selective and nonselective media were negative for Neisseria gonorrhoeae. Among examined endocervical swabs, 13samples (1.18%) were positive by nucleic acid amplification of Neisseria gonorrgoeae cppB gene. Conclusion: Negative results of culture and positive results of PCR in this study indicate that however culture is gold standard method for detection of Neisseria gonorrhoeae but due to bacterial autolysis, poor sampling techniques and improper specimen storage and transport, its value decline as compared with Nucleic acid amplification test (NAAT).

Keywords: Neisseria gonorrgoeae, pregnant women, culture, PCR
Full-Text [PDF 122 kb]   (1678 Downloads)    
Type of Study: Original | Subject: General
Received: 2013/10/20 | Accepted: 2013/10/20 | Published: 2013/10/20


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Volume 16, Issue 5 (Iranian South Medical Journal 2013) Back to browse issues page