Background: Today the consequences of taking metal ions especially heavy metals on human health and the environment is of great interest, especially for aquatic food products. The main aim of this scientific and applied research was to measure, some ionic metals’ concentration (i.e. Ni, Fe, V, Co, Cr, Ag, Cd, Li and Ba) in Shrimp Penaeus semisulcatus collected from Persian Gulf. Materials and Methods: In this research twenty one samples of Shrimp Penaeus semisulcatus from seven regional fishing ports in Bandar Bushehr, Bandar Mahshahr and Bandar Abbass were collected and transferred to the laboratory in an ice box immediately. After sample preparation according to the AOAC method, each sample was introduced into the inductively coupled plasma atomic emission spectrometer (ICP-OES) for determination of the metallic elements’ concentration. Results: The results showed that the average concentration of all elements except of vanadium in the muscle of shrimp was higher than the skin. Statistical analysis showed significant differences in the mean of cobalt and lithium accumulation in the skin and muscle of shrimp (P <0/05). Also mean concentration of metals measured in the muscle and skin of shrimps collected from Bushehr have the highest amount of metallic pollution compared to the other two fishing regions which could be a sign of potential contamination of this aquatic area. Conclusion : The comparison of mean concentration in muscle of collected shrimps from Persian Gulf with the WHO recommended guidlines showed that the concentration of metallic elements are lower than the WHO allowable limits and there is no concern regarding consumption of these products.