:: Volume 17, Issue 4 (Iranian South Medical Journal 2014) ::
Iran South Med J 2014, 17(4): 542-549 Back to browse issues page
Analysis of G>A change in splicing site of intron 6 of XRCC4 gene in patients with differentiated thyroid cancer (DTC)
Maryam Rahimi1, Pzhman Fard-Esfahani *2, Shima Fayaz3, Armghan Fard-Esfahani4, Mohammed Hussain Modarressi5, Seyed Mohammad Akrami5
1- Faculty of Basic Sciences, Science and Research Branch of Islamic Azad University, Tehran, IRAN
2- Medical Biotechnology, Biochemistry Dept., Pasteur Institute of Iran, Tehran, IRAN , fard-esfahani@pasteur.ac.ir
3- Medical Biotechnology, Biochemistry Dept., Pasteur Institute of Iran, Tehran, IRAN
4- Nuclear Medicine Specialist, Research Institute for Nuclear Medicine, Tehran University of Medical Sciences, Tehran, IRAN
5- Genetic group Medical College of medicine, Tehran University of Medical Sciences, Tehran, IRAN
Abstract:   (5464 Views)

Background: Differentiated thyroid carcinoma (DTC) is the most prevalent thyroid neoplasm which includes papillary and follicular cell carcinoma. Exposure to ionizing radiation is a predisposing factor for developing DTC. Non Homologous End Joining (NHEJ) DNA repair pathway is important one among DNA repair pathways which rejoins ends of broken DNA strands. XRCC4 gene is one of the most important genes in this pathway and G>A polymorphism in acceptor site of splicing site of its intron, causes truncated protein production. The aim of this study was assay presence any relationship between this polymorphism and differentiated thyroid carcinoma (DTC). Material and Methods: In this case-control study, by using PCR-RFLP method, rs1805377 SNP of XRCC4 gene was analyzed in total of 172 DTC patients and 195 cancer free individuals who admitted in Shariate Hospital of Tehran. The frequencies of this single nucleotide polymorphism (SNP) in case and control groups were compared. Also, risk ratio of having DTC in genotypes was assyed using regression analysis. Results: Current results showed no significant differences in genotypes between case and control groups (p-value: 0.588, OR= 1.52, 95% CI: 0.3349-6.9638). Also, dichotomized genotypes in DTC and control groups showed no significant results. Conclusion: Although the results showed potential association of A genotype with DTC risk, but these findings should be replicated in other studies with larger sample sizes.

Keywords: Differentiated thyroid carcinoma (DTC), Non Homologous End Joining (NHEJ), single nucleotide polymorphism (SNP), NHEJ, XRCC4 gene
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Type of Study: Original | Subject: General
Received: 2012/04/7 | Accepted: 2012/09/5 | Published: 2014/08/11


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Volume 17, Issue 4 (Iranian South Medical Journal 2014) Back to browse issues page