:: Volume 18, Issue 4 (Iranian South Medical Journal 2015) ::
Iran South Med J 2015, 18(4): 720-728 Back to browse issues page
Evaluation of prevalence of Pseudomonas aeruginosa infection in operated patients with Chronic Sinusitis in Rasoule Akram Hospital by Polymerase Chain Reaction (PCR)
Hania Shafienia1, Mohammad Hasan Shahhosseiny 2, Mansour Bayat3, Mohammad amin Mahmoudi4, Mehrdad Rabiei Nematabad4, Mohammad Farhadi5
1- Department of Biology, Science and Research Branch, Islamic Azad University,Tehran, Iran
2- Department of Microbiology, Shahr-e-Qods branch, Islamic Azad University, Tehran, Iran
Iranian Gene Fanavar Institute (IGF), Tehran, Iran , shahosseiny@yahoo.com
3- Department of veterinary Mycology , Science and research branch , Islamic Azad university, Tehran,Iran
4- Iranian Gene Fanavar Institute (IGF), Tehran, Iran
5- Research Center for Diseases of Ear, Nose and Throat, Iran University of Medical Sciences, Tehran, Iran
Abstract:   (4299 Views)

Background: Chronic sinusitis(CS) is one of the most prevalent chronic illnesses that affecting persons of all age groups. It is an inflammatory process that involves the paranasal sinuses. There isn't definitive and consistent data concerning the distribution of bacterial species in patients with Chronic Sinusitis. Pseudomonas aeruginosa has emerged as a potential pathogen in the immunocompromised patients, so this microorganism is one of the most important cause of Chronic sinusitis. The purpose of this study is molecular detection of sinusitis caused by P.aeruginosa. Material and Methods: 50 specimens was provided from the secretion of maxillary and frontal sinuses of patients from Rasoule Akram hospital during operation. Genomic bacterial DNA was extracted by DNP kit and detection of this bacteria was proceeded by employing sequence-specific target namely the outer membrane protein (oprL) gene locus and designing primers. PCR optimized and sensitivity and specificity tests was performed. Amplicon was cloned by T/A Cloning method and was used for sequencing and positive control. Results: The product of optimized PCR with 504 bp length correctly amplified and observed on electrophoreses gel 1.5% and confirmed by sequencing. Evaluation of the selected primers with 8 various DNA demonstrated 100% specificity. Sensitivity of optimized test was Evaluated 10 CFU of bacteria. From the 50 samples, 22% of specimens were positive for P.aeruginosa. Conclusion: This study indicates that molecular detection of P.aeruginosa employing the oprL gene target is a rapid and useful technique for detection of P.aeruginosa.

Keywords: Sinusitis, Pseudomonas aeruginosa, Polymerase Chain Reaction, Molecular Diagnosis
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Type of Study: Original | Subject: Microbiology and Immunology
Received: 2014/03/11 | Accepted: 2014/08/13 | Published: 2015/09/12


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