:: Volume 19, Issue 4 (Iranian South Medical Journal 2016) ::
Iran South Med J 2016, 19(4): 511-525 Back to browse issues page
Antioxidant effects of alpha-tocopherol (vitamine E) on testis regeneration in busulfan-treated mice
Fatemeh Rohnavaz1, Toba Mirzapour 2, Abolfazl Bayrami1, Maria Zahiri3
1- Department of biology, Faculty of Science, University of Mohaghegh Ardabili, Ardabil, Iran
2- Department of biology, Faculty of Science, University of Mohaghegh Ardabili, Ardabil, Iran , Dr.tooba72@gmail.com
3- Department of Anatomical Sciences, School of Medicine, Bushehr University of Medical Sciences, Bushehr, Iran
The Persian Gulf Marine Biotechnology Research Center, The Persian Gulf Biomedical Sciences Research Institute, Bushehr University of Medical Sciences, Bushehr, Iran
Abstract:   (4828 Views)

Background: Busulfan is a chemotherapy medicine for treatment of cancer. This medicine has cytotoxic effects on different organs of animal. Since the use of chemotherapy medicine are increasing due to individuals get involved to cancer, thus preserving of  reproductive system in these patients is essential to continue fertility.

Materials and Method: In present study the testis of mice  were  injected with different doses of busulfan
(35, 40 mg/kg body weight intraperitonealy. The effects of busulfan was examinned on destruction of testis tissue, semen parameters and catalase enzymatic activity compared with control group after 30 days. Finally, fter determining the effective dose of busulfan, Vitamin E was injected to the mice as an antioxidant enzyme  and its effects on semen parameters and testis tissue was examined after one month. 

Results: The results showed that oxidative stress was increased in busulfan-treated mice during 30 days. Catalase enzymatic activity decreased significantly in testis of Busulfan-treated mice in comparison with control group. A significant decrease of sperm number was observed according to treatment by Busulfan. After finding the most damaging dose of busulfan (40 mg/kg ) in destruction of testis tissue, these mice were injected by vitamine E. The results showed that the oxidative stress and percentage of abnormal sperm were decreased in Busulfan-treated mice that exposed a dose of 100 mg/kg vitamin E. The number of sperm and antioxidant activity of catalase was increased.

Conclusion: Vitamin E improves a revival of spermatogenesis and improves testicular parameters in these patients. 

Keywords: Busulfan, Vitamin E, Antioxidant, Catalase, Semen Parameter
Full-Text [PDF 990 kb]   (1864 Downloads)    
Type of Study: Original | Subject: Biochemistry. Cell Biology and Genetics
Received: 2016/09/7 | Accepted: 2016/09/7 | Published: 2016/09/7
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