:: Volume 19, Issue 6 (Iranian South Medical Journal 2017) ::
Iran South Med J 2017, 19(6): 940-950 Back to browse issues page
Methylation Status of NANOG1, RASSF1A, SFN, CASP8, WIF1,CTSL2 Genes in Iranian Breast Cancer Patients
Ali Motevalizadeh Ardekani 1, Mahsa Shirani2, Ahmad Hashemi2, Zohre Basiri2, Yazdan Rahmati2, Novin Nikbakhsh3, Sahar Edrisi3, Shahla Mohammadganji2
1- Department of Medical Biotechnology, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran , Ardekani@nigeb.ac.ir
2- Department of Medical Biotechnology, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran
3- Cancer Research Center, Babol University of Medical Sciences, Babol, Iran
Abstract:   (5750 Views)

Background: Mammographic screening to diagnose the breast cancer showe false-negative and false-positive results in young women and therefore a non-invasive and low cost method is needed, to diagnose the breast cancer in the early stages. DNA methylation changes are the most common molecular changes in human cancers and including breast cancer. Therefore, The pattern of tissues methylation can be used in the early diagnosis of cancer. Also similar methylation patterns found in tumors and in plasma shows potential application of molecular detection of breast cancer, based on blood. The aim of this study was to assess the promoter methylation for the clinical diagnosis of breast cancer.

Material and Methods: To examine the promoter methylation 21 tumor tissues and 21 normal tissues have been studied for clinical diagnosis of breast cancer. 6 gene methylation status (NANOG1, RASSF1A, SFN, CASP8, WIF1 and CTSL2) was analyzed by methylation specific PCR (MS-PCR).

Results: The results show that NANOG gene was methylated in 94.7% of tumor specimen and 100% of normal specimen, RASSF1A gene was methylated in 9.5% of tumor specimen and 0% of normal specimen, SFN gene was methylated in 14.3% of tumor specimen and 27.8% of normal specimen, CASP8 gene was methylated in 30% of tumor specimen and 23.5% of normal specimen, WIF1 gene was methylated in 80% of tumor specimen and 27.8% of normal specimen, CTSL2 gene was methylated in 28.6% of tumor specimen and 23.5 % of normal specimen were methylated. Data analysis did not show a significant relationship between these results. (P >0.05).

Conclusion: The results of this study demonstrate that 6 gene methylation status was not enough to differentiate between the cancer and normal groups. This study demonstrates the methodological problems (MS PCR) which was used to assess the methylation markers to evaluate the methylation status as diagnostic biomarkers. 

Keywords: DNA methylation, breast cancer, MS-PCR, NANOG1, RASSF1A, SFN, CASP8, WIF1, CTSL2 genes
Full-Text [PDF 879 kb]   (1613 Downloads)    
Subject: Biochemistry. Cell Biology and Genetics
Received: 2016/12/27 | Accepted: 2016/12/27 | Published: 2016/12/27
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Volume 19, Issue 6 (Iranian South Medical Journal 2017) Back to browse issues page