:: Volume 20, Issue 3 (Iranian South Medical Journal 2017) ::
Iran South Med J 2017, 20(3): 257-266 Back to browse issues page
Investigation of Association Between KISS1R rs397515615 Polymorphism with Idiopathic Male Infertility
Azadeh Poursharif1, Hamidreza Vaziri 2, Toba Mirzapour3
1- Department of Biology, School of Science, University of Guilan, Guilan, Iran
2- Department of Biology, School of Science, University of Guilan, Guilan, Iran , vaziri@guilan.ac.ir
3- Department of Biology, School of Science, University of Mohaghegh Ardabili, Ardabil, Iran
Abstract:   (3147 Views)

Background: Infertility refers to the inability of a couple to conceive over an average period of one year with unprotected sexual intercourse. In mammals, Fertility is initiated at puberty by the pulsatile secretion of the gonadotropin-releasing hormone (GnRH). Kisspeptin (product of Kiss1 gene) and its receptor (KISS1R) are as vital upstream regulators in integrating of central and peripheral signals with GnRH release. In this paper, we studied an association between polymorphism rs 397515615 in the KISS1R gene and idiopathic male infertility.
Material and Methods: In this research, we evaluated a total of 50 men with idiopathic infertility and 50 healthy men (control group). The AS-PCR method was applied for the determination of the codon polymorphism. Also, we used chi-square (χ2) test to study of an association between genotype and allele frequencies in cases and control groups.
Results: The results showed that the genotypic frequencies between two groups revealed a significant difference between the patients and control group (P=0.02), while the allele distribution (G/-) between case and control groups showed no significant difference (P=0.20).
Conclusion: The results of this study show that the polymorphism of rs 397515615 KISS1R gene probably associated with idiopathic infertility in men. However, further studies with larger sample size and diverse geographical distribution are required for more conclusive results.

Keywords: rs 397515615, KISS1R gene, Male-factor Infertility, Polymorphism
Full-Text [PDF 720 kb]   (771 Downloads)    
Type of Study: Original | Subject: Biochemistry. Cell Biology and Genetics
Received: 2016/09/21 | Accepted: 2016/10/26 | Published: 2017/07/5
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