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:: Volume 20, Issue 5 (Iranian South Medical Journal 2017) ::
Iran South Med J 2017, 20(5): 426-436 Back to browse issues page
Molecular Typing Isolates of Salmonella Enterica Serovar Infantis Using Eric-PCR Method
Abolfazl Moghadam 1, Shahram Nazarian *2
1- Department of Cell and Molecular Science, School of Biology, University of tehran, Tehran, Iran
2- Department of Biology, School of Science, Imam Hossain University, Tehran, Iran , kpnazari@ihu.ac.ir
Abstract:   (89 Views)
Background: Salmonella is the important bacteria of the Enterobacteriaceae family that are very diverse biochemically and serologically, and mainly transmitted through food. The spread of non-typhoid Salmonella is one of the challenging issues in current research. Therefore rapid diagnosis and typing of the pathogensprovide efficient information about detection and controlling of infection. The aim of this study is typing the clinical strains of Salmonella Infantis.
Materials and Methods: In this study, strains of Salmonella Infantis were isolated from different health centers. All of the strains were identified by standard microbiology, biochemical and molecular methods. Genetic relationship between strains was analyzed by ERIC-PCR method.
Results: In this study, from 842 stool and blood sample of patients with diarrhea, 48 different strains were isolated which related to Salmonella Infantis. Strains categorized into 14 different groups by genotyping using ERIC-PCR method that highest number of the strains were placed in group 5 (20%, 10 strains).
Conclusion: Results of this study demonstrated that strains of Salmonella Infantis which are examined genetically were diverse which can be due to the prevalence of polyclonal strains in human samples. It was also shown that ERIC-PCR method has great differential power for the molecular typing.
 
Keywords: Salmonella Infantis, ERIC-PCR, Genetic diversity, molecular typing
Full-Text [PDF 729 kb]   (88 Downloads)    
Type of Study: Original | Subject: Microbiology and Immunology
Received: 2017/11/13 | Accepted: 2017/11/13 | Published: 2017/11/13
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Moghadam A, Nazarian S. Molecular Typing Isolates of Salmonella Enterica Serovar Infantis Using Eric-PCR Method. Iran South Med J. 2017; 20 (5) :426-436
URL: http://ismj.bpums.ac.ir/article-1-896-en.html
Volume 20, Issue 5 (Iranian South Medical Journal 2017) Back to browse issues page
دانشگاه علوم پزشکی بوشهر، طب جنوب ISMJ

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