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:: Volume 20, Issue 5 (Iranian South Medical Journal 2017) ::
Iran South Med J 2017, 20(5): 437-447 Back to browse issues page
Study of Association Between Polymorphism Alpha7 Neuronal Nicotinic Acetylcholine Receptor Subunit Gene (CHRNA7) with the Development of Schizophrenia in Iranian Population
Shadi Haghighat 1, Rasoul Zahmatkesh Roodsari 2, Reza Ranjbar 3
1- Department of Genetics, School of Biological Sciences, Tonekabon Branch, Islamic Azad University, Tonekabon, Iran
2- Department of Biology, School of Biological Sciences, Tonekabon Branch, Islamic Azad University, Tonekabon, Iran , rasoul130@yahoo.com
3- Molecular Biology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran
Abstract:   (1115 Views)
Background: SCZ (Schizophrenia) is a common psychiatric and mental in which patients share a common genetic vulnerability. The α7 neuronal nicotinic receptor gene (CHRNA7) is located on the chromosome 15q13-q14. There are several single nucleotide polymorphisms (SNPs) in exons, introns and promoter sites inside the CHRNA7 gene. The aim of this research was to investigate the association between polymorphism CHRNA7 and the development of schizophrenia in Iranian population.
Materials and Methods: This case-control study included 100 patients with schizophrenia and 100 healthy volunteers as a control group. Genomic DNA was extracted from blood samples. Genotypes were determined using the PCR-RFLP method. Statistical analysis was performed using the SPSS software version 20.
Results: Frequency of CC, TCand TT genotypes were 18%, 42% and 40% in patients group  and 14%, 45% and 41%, in normal groups respectively. Statistical analysis indicated that in the total sample including male and female, there was no significant association between CHRNA7 gene polymorphism and schizophrenia disease.
Conclusion: The presence of the T allele in CHRNA7 gene at rs904942 cannot be considered as a risk factor for schizophrenia. Therefore, further genetic studies with more SNPs and larger sample sizes with more heterogenicity are required for more conclusive results.
 
Keywords: Schizophrenia, polymorphism, CHRNA7, PCR-RFLP
Full-Text [PDF 908 kb]   (250 Downloads)    
Type of Study: Original | Subject: Nervous System
Received: 2017/11/13 | Accepted: 2017/11/13 | Published: 2017/11/13
References
1. Stewart LR, Hall AL, Kang SH, et al. High frequency of known copy number abnormalities and maternal duplication 15q11-q13 in patients with combined schizophrenia and epilepsy. BMC Med Genet 2011;12: 154. [DOI:10.1186/1471-2350-12-154]
2. Toulopoulou T, Picchioni M, Rijsdijk F, et al. Substantial genetic overlap between neurocognition and schizophrenia: genetic modeling in twin samples. Arch Gen Psychiatry 2007; 64(12): 1348-55. [DOI:10.1001/archpsyc.64.12.1348]
3. Stefansson H, Ophoff RA, Steinberg S, et al. Common variants conferring risk of schizophrenia. Nature 2009; 460(7256): 744-7 [DOI:10.1038/nature08186]
4. Zhou D, Gochman P, Broadnax DD, et al. 15q13.3 duplication in two patients with childhood-onset schizophrenia. Am J Med Genet B Neuropsychiatr Genet 2016;171(6): 777-83. [DOI:10.1002/ajmg.b.32439]
5. Freund RK, Graw S, Choo KS, et al. Genetic knockout of the α7 nicotinic acetylcholine receptor gene alters hippocampal long-term potentiation in a background strain-dependent manner. Neurosci lett 2016; 627: 1-6. [DOI:10.1016/j.neulet.2016.05.043]
6. Gass N, Weber-Fahr W, Sartorius A, et al. An acetylcholine alpha7 positive allosteric modulator rescues a schizophrenia-associated brain endophenotype in the 15q13.3 microdeletion, encompassing CHRNA7. Eur Neuropsychopharmacol 2016; 26(7): 1150-60 [DOI:10.1016/j.euroneuro.2016.03.013]
7. Parikh V, Kutlu MG, Gould TJ. nAChR dysfunction as a common substrate for schizophrenia and comorbid nicotine addiction: Current trends and perspectives. Schizophr Res 2016; 171(1-3): 1-15.
8. Klemettilä J-P, Kampman O, Solismaa A, et al. Association study of arcuate nucleus neuropeptide Y neuron receptor gene variation and serum NPY levels in clozapine treated patients with schizophrenia. Eur Psychiatry 2017; 40: 13-9. [DOI:10.1016/j.eurpsy.2016.07.004]
9. Nadalin S, Ristić S, Rebić J, et al. The insertion/deletion polymorphism in the angiotensin-converting enzyme gene and nicotine dependence in schizophrenia patients. J Neural Transm (Vienna) 2017; 124(4): 511-8. [DOI:10.1007/s00702-016-1670-y]
10. Klemettilä JP. Metabolic adverse effects in clozapine-treated patients with schizophrenia: Cytokine and adipokine alterations, neuropeptide Y, and genetic associations.
11. 2016 ,978-952-03-0293-1
12. García I. Bases genéticas de la discapacidad intelectual y los trastornos del espectro autista: aplicación de las nuevas tecnologías al análisis de variantes del número de copias (CNVs). 2017-01-17T11:31:09Z
13. Firouzabadi SG, Karimine jad R, Vameghi R, et al. Copy number variants in patients with autism and additional clinical features: report of VIPR2 duplication and a novel microduplication Syndrome. Mol Neurobiol. In press 2016.
14. Bulayeva K, Bulayev O, Glatt S. Genomic Architecture of Schizophrenia Across Diverse Genetic Isolates: A Study of Dagestan Populations. New York City: Springer, 2016, 978-3-319-31964-3. [DOI:10.1007/978-3-319-31964-3]
15. Lomartire S. Analysis of Copy Number Variants identifies new candidate genes for Autism Spectrum Disorder and Intellectual Disability. Alma, 2015.
16. Callicott JH, Straub RE, Pezawas L, et al. Variation in DISC1 affects hippocampal structure and function and increases risk for schizophrenia. Proc Natl Acad Sci U S A 2005; 102(24): 8627-32. [DOI:10.1073/pnas.0500515102]
17. Sinkus ML, Graw S, Freedman R, et al. The human CHRNA7 and CHRFAM7A genes: A review of the genetics, regulation, and function. Neuropharmacology 2015; 96(Pt B): 274-88.
18. Bakanidze G, Roinishvili M, Chkonia E, et al. Association of the nicotinic receptor α7 subunit gene (CHRNA7) with schizophrenia and visual backward masking. Front Psychiatry 2013; 4: 133. [DOI:10.3389/fpsyt.2013.00133]
19. Zammit S, Spurlock G, Williams H, et al. Genotype effects of CHRNA7, CNR1 and COMT in schizophrenia: interactions with tobacco and cannabis use. Br J Psychiatry 2007; 191: 402-7. [DOI:10.1192/bjp.bp.107.036129]
20. DH, Scoles HA, Horike S-i, et al. 15q11.2-13.3 chromatin analysis reveals epigenetic regulation of CHRNA7 with deficiencies in Rett and autism brain. Hum Mol Genet 2011; 20(22): 4311-23. [DOI:10.1093/hmg/ddr357]
21. Joo EJ, Lee KY, Kim HS, et al. Genetic association study of the alpha 7 nicotinic receptor (CHRNA7) with the development of schizophrenia and bipolar disorder in Korean population. Psychiatry Investig 2010; 7(3): 196-201. [DOI:10.4306/pi.2010.7.3.196]
22. Neves-Pereira M, Bassett AS, Honer WG, et al. No evidence for linkage of the CHRNA7 gene region in canadian schizophrenia families. Am J Med Genet 1998; 81(5): 361-3. https://doi.org/10.1002/(SICI)1096-8628(19980907)81:5<361::AID-AJMG3>3.0.CO;2-W [DOI:10.1002/(SICI)1096-8628(19980907)81:53.0.CO;2-W]
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Haghighat S, Zahmatkesh Roodsari R, Ranjbar R. Study of Association Between Polymorphism Alpha7 Neuronal Nicotinic Acetylcholine Receptor Subunit Gene (CHRNA7) with the Development of Schizophrenia in Iranian Population. Iran South Med J. 2017; 20 (5) :437-447
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Volume 20, Issue 5 (Iranian South Medical Journal 2017) Back to browse issues page
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