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:: Volume 20, Issue 5 (Iranian South Medical Journal 2017) ::
Iran South Med J 2017, 20(5): 481-491 Back to browse issues page
Purification of Bysal Mussel (Modiolus sp. PG) Adhesive Protein fp-2 from Nortern Seashore of Persian Gulf
Saba Hosseini 1, Hossean Zolgharnine * 2, Afshar Bargahi 3, Hoda Khaledi 1, Negin Salamat 1, Bita Archangi 1
1- Department of Marine Biology, School of Marine Science and Ocean, Khorramshahr University of Marine Science and Technology, Khorramshahr, Iran
2- Department of Marine Biology, School of Marine Science and Ocean, Khorramshahr University of Marine Science and Technology, Khorramshahr, Iran , Zolgharnine@yahoo.com
3- The Persian Gulf Marine Biotechnology Research Center, The Persian Gulf Biomedical Sciences Research Institute, Bushehr University of Medical Sciences, Bushehr, Iran
Abstract:   (578 Views)
Background: Mussel bivalves produce and secrete adhesive proteins which facilitate the adherence to surfaces in the aquatic environments. Specific properties of these proteins such as adhesion, resistance and biocompatibility have introduced their application in biomedical engineering, dental, and production of biological adhesives.
Materials and Methods: The species of dwarves from the Persian Gulf were sampled based on the morphologic indexes and COI gene sequence. To identify the polyphenol glands, which produce the adhesive proteins, tissue sections were prepared from the mussel’s foot. Then, the mussel fp-2 protein was purified by the ion-exchange chromatography using salt and pH gradients and, the gel filtration chromatography. To confirm the presence of proteins in each fraction the Arnow test and NBT staining were used. For detection of the protein homogeneity, in each purification stage, the SDS-PAGE electrophoresis was used. The NBT positive chromatogram peaks, after concentration and electrophoresis by Acid-Urea method, were blotted on nitrocellulose and detected by NBT test.
Results: The specie morphology and taxonomy were recorded and matched to the FAO criteria and, "The Mollusks of Persian Gulf", the Persian reference book. It belonged to the Mytilidae family and Modiolus genus. The DNA and protein sequences were recorded in NCBI data bank as AB972412.1 and BAP16437.1 respectively. Also, the molecular weight of the purified fp-2 protein was about 45 KD.
Conclusion: This research was led to the morphologic and genetic identification of Modiolus sp. PG from the Persian Gulf along with the purification of the fp-2 adhesive protein.
 
Keywords: Adhessive protein, COI gene, Extraction, fp-2 protein, Modiolus sp. PG
Full-Text [PDF 764 kb]   (169 Downloads)    
Type of Study: Original | Subject: Disorders of Systemic, Metabolic or Environmental Origin
Received: 2017/11/13 | Accepted: 2017/11/13 | Published: 2017/11/13
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Hosseini S, Zolgharnine H, Bargahi A, Khaledi H, Salamat N, Archangi B. Purification of Bysal Mussel (Modiolus sp. PG) Adhesive Protein fp-2 from Nortern Seashore of Persian Gulf. Iran South Med J. 2017; 20 (5) :481-491
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Volume 20, Issue 5 (Iranian South Medical Journal 2017) Back to browse issues page
دانشگاه علوم پزشکی بوشهر، طب جنوب ISMJ

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