Volume 20, Issue 5 (Iranian South Medical Journal 2017)
Iran South Med J 2017, 20(5): 481-491 |
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Saba Hosseini1 
, Hossean Zolgharnine * 2, Afshar Bargahi3 , Hoda Khaledi1 , Negin Salamat1 , Bita Archangi1
, Hossean Zolgharnine * 2, Afshar Bargahi3 , Hoda Khaledi1 , Negin Salamat1 , Bita Archangi1
1- Department of Marine Biology, School of Marine Science and Ocean, Khorramshahr University of Marine Science and Technology, Khorramshahr, Iran
2- Department of Marine Biology, School of Marine Science and Ocean, Khorramshahr University of Marine Science and Technology, Khorramshahr, Iran , Zolgharnine@yahoo.com
3- The Persian Gulf Marine Biotechnology Research Center, The Persian Gulf Biomedical Sciences Research Institute, Bushehr University of Medical Sciences, Bushehr, Iran
2- Department of Marine Biology, School of Marine Science and Ocean, Khorramshahr University of Marine Science and Technology, Khorramshahr, Iran , Zolgharnine@yahoo.com
3- The Persian Gulf Marine Biotechnology Research Center, The Persian Gulf Biomedical Sciences Research Institute, Bushehr University of Medical Sciences, Bushehr, Iran
Abstract: (4158 Views)
Background: Mussel bivalves produce and secrete adhesive proteins which facilitate the adherence to surfaces in the aquatic environments. Specific properties of these proteins such as adhesion, resistance and biocompatibility have introduced their application in biomedical engineering, dental, and production of biological adhesives.
Materials and Methods: The species of dwarves from the Persian Gulf were sampled based on the morphologic indexes and COI gene sequence. To identify the polyphenol glands, which produce the adhesive proteins, tissue sections were prepared from the mussel’s foot. Then, the mussel fp-2 protein was purified by the ion-exchange chromatography using salt and pH gradients and, the gel filtration chromatography. To confirm the presence of proteins in each fraction the Arnow test and NBT staining were used. For detection of the protein homogeneity, in each purification stage, the SDS-PAGE electrophoresis was used. The NBT positive chromatogram peaks, after concentration and electrophoresis by Acid-Urea method, were blotted on nitrocellulose and detected by NBT test.
Results: The specie morphology and taxonomy were recorded and matched to the FAO criteria and, "The Mollusks of Persian Gulf", the Persian reference book. It belonged to the Mytilidae family and Modiolus genus. The DNA and protein sequences were recorded in NCBI data bank as AB972412.1 and BAP16437.1 respectively. Also, the molecular weight of the purified fp-2 protein was about 45 KD.
Conclusion: This research was led to the morphologic and genetic identification of Modiolus sp. PG from the Persian Gulf along with the purification of the fp-2 adhesive protein.
Materials and Methods: The species of dwarves from the Persian Gulf were sampled based on the morphologic indexes and COI gene sequence. To identify the polyphenol glands, which produce the adhesive proteins, tissue sections were prepared from the mussel’s foot. Then, the mussel fp-2 protein was purified by the ion-exchange chromatography using salt and pH gradients and, the gel filtration chromatography. To confirm the presence of proteins in each fraction the Arnow test and NBT staining were used. For detection of the protein homogeneity, in each purification stage, the SDS-PAGE electrophoresis was used. The NBT positive chromatogram peaks, after concentration and electrophoresis by Acid-Urea method, were blotted on nitrocellulose and detected by NBT test.
Results: The specie morphology and taxonomy were recorded and matched to the FAO criteria and, "The Mollusks of Persian Gulf", the Persian reference book. It belonged to the Mytilidae family and Modiolus genus. The DNA and protein sequences were recorded in NCBI data bank as AB972412.1 and BAP16437.1 respectively. Also, the molecular weight of the purified fp-2 protein was about 45 KD.
Conclusion: This research was led to the morphologic and genetic identification of Modiolus sp. PG from the Persian Gulf along with the purification of the fp-2 adhesive protein.
Type of Study: Original |
Subject:
Disorders of Systemic, Metabolic or Environmental Origin
Received: 2016/12/29 | Accepted: 2017/04/19 | Published: 2017/11/13
Received: 2016/12/29 | Accepted: 2017/04/19 | Published: 2017/11/13
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