fa تأثیر اسید‌فولیک بر یادگیری و حافظه فضایی در مدل تخریب الکتریکی هسته‌ی قاعده‌ای مگنوسلولاریس: مدل حیوانی بیماری آلزایمر فیزیولوژی Physiology پژوهشي Original <div style="text-align: justify;"><span style="font-size:12pt"><span style="line-height:115%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:10.0pt"><span style="line-height:115%"><span iransans="" style="font-family:"><span style="color:#1f4e79">زمینه:</span></span></span></span></b> <span lang="FA" style="font-size:10.0pt"><span style="line-height:115%"><span iransans="" style="font-family:"><span style="color:#0d0d0d">بیماری آلزایمر (</span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:115%"><span iransans="" style="font-family:"><span style="color:#0d0d0d">AD</span></span></span></span><span lang="FA" style="font-size:10.0pt"><span style="line-height:115%"><span iransans="" style="font-family:"><span style="color:#0d0d0d">) یک بیماری نورودژنراتیو همراه با اختلال شناختی شدید است. با در نظر گرفتن نقش اسید‌فولیک (</span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:115%"><span iransans="" style="font-family:"><span style="color:#0d0d0d">FA</span></span></span></span><span lang="FA" style="font-size:10.0pt"><span style="line-height:115%"><span iransans="" style="font-family:"><span style="color:#0d0d0d">) بر بهبود عملکردهای شناختی در برخی از مدل‌های بیماری، مطالعه حاضر با هدف بررسی تأثیر اسید‌فولیک بر اختلال یادگیری و حافظه فضایی القاء شده توسط تخریب الکتریکی دو طرفه هسته قاعده‌ای مگنوسلولاریس (</span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:115%"><span iransans="" style="font-family:"><span style="color:#0d0d0d">NBM</span></span></span></span><span lang="FA" style="font-size:10.0pt"><span style="line-height:115%"><span iransans="" style="font-family:"><span style="color:#0d0d0d">) در موش‌های صحرایی نر بالغ مدل بیماری آلزایمر انجام شده است.</span></span></span></span></span></span></span></span></span></div> <span style="font-size:12pt"><span style="line-height:115%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:10.0pt"><span style="line-height:115%"><span iransans="" style="font-family:"><span style="color:#1f4e79">مواد و روش‌ها:</span></span></span></span></b><b> </b><span lang="FA" style="font-size:10.0pt"><span style="line-height:115%"><span iransans="" style="font-family:"><span style="color:#0d0d0d">در این مطالعه تجربی 49 موش صحرایی نر بالغ نژاد ویستار به‌طور تصادفی به 7 گروه تقسیم شدند: کنترل، تخریب </span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:115%"><span iransans="" style="font-family:"><span style="color:#0d0d0d">NBM</span></span></span></span><span lang="FA" style="font-size:10.0pt"><span style="line-height:115%"><span iransans="" style="font-family:"><span style="color:#0d0d0d"> (تخریب الکتریکی دو‌طرفه </span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:115%"><span iransans="" style="font-family:"><span style="color:#0d0d0d">NBM</span></span></span></span><span lang="FA" style="font-size:10.0pt"><span style="line-height:115%"><span iransans="" style="font-family:"><span style="color:#0d0d0d">)، شاهد تخریب (ورود الکترود به </span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:115%"><span iransans="" style="font-family:"><span style="color:#0d0d0d">NBM</span></span></span></span><span style="font-size:10.0pt"><span style="line-height:115%"><span iransans="" style="font-family:"><span style="color:#0d0d0d"> بدون القاء جریان الکتریکی)، حلال (تخریب+ سالین)، تخریب+ اسید‌فولیک (5، 10 و 15 میلی‌گرم بر کیلوگرم). در گروه‌های تحت تیمار 30 دقیقه بعد از تخریب </span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:115%"><span iransans="" style="font-family:"><span style="color:#0d0d0d">NBM</span></span></span></span><span lang="FA" style="font-size:10.0pt"><span style="line-height:115%"><span iransans="" style="font-family:"><span style="color:#0d0d0d"> تزریق داخل صفاقی اسید‌فولیک و یا سالین به مدت یک هفته انجام شد. سپس به مدت 5 روز بوسیله ماز </span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:115%"><span iransans="" style="font-family:"><span style="color:#0d0d0d">Y</span></span></span></span><span style="font-size:10.0pt"><span style="line-height:115%"><span iransans="" style="font-family:"><span style="color:#0d0d0d"> شکل آموزش دیدند. بیست و پنج روز بعد از آموزش نیز آزمون فراخوانی حافظه برای ارزیابی حافظه بلند مدت انجام گرفت.</span></span></span></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="line-height:115%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:10.0pt"><span style="line-height:115%"><span iransans="" style="font-family:"><span style="color:#1f4e79">یافته‌ها:</span></span></span></span></b> <span lang="FA" style="font-size:10.0pt"><span style="line-height:115%"><span iransans="" style="font-family:"><span style="color:#0d0d0d">تخریب دوطرفه </span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:115%"><span iransans="" style="font-family:"><span style="color:#0d0d0d">NBM</span></span></span></span><span style="font-size:10.0pt"><span style="line-height:115%"><span iransans="" style="font-family:"><span style="color:#0d0d0d"> منجر به کاهش یادگیری و حافظه فضایی در مقایسه با گروه‌های کنترل و شاهد تخریب گردید (0/001</span></span></span></span><span lang="FA" style="font-size:10.0pt"><span style="line-height:115%"><span arial="" style="font-family:"><span style="color:#0d0d0d">˂</span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:115%"><span iransans="" style="font-family:"><span style="color:#0d0d0d">P</span></span></span></span><span lang="FA" style="font-size:10.0pt"><span style="line-height:115%"><span iransans="" style="font-family:"><span style="color:#0d0d0d">). افزایش معنی‌دار یادگیری و حافظه فضایی در گروه‌های تخریب +10 میلی‌گرم بر کیلوگرم اسید فولیک (0/05</span></span></span></span><span lang="FA" style="font-size:10.0pt"><span style="line-height:115%"><span arial="" style="font-family:"><span style="color:#0d0d0d">˂</span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:115%"><span iransans="" style="font-family:"><span style="color:#0d0d0d">P</span></span></span></span><span lang="FA" style="font-size:10.0pt"><span style="line-height:115%"><span iransans="" style="font-family:"><span style="color:#0d0d0d">) و تخریب +15 میلی‌گرم بر کیلوگرم اسید فولیک (0/01</span></span></span></span><span lang="FA" style="font-size:10.0pt"><span style="line-height:115%"><span arial="" style="font-family:"><span style="color:#0d0d0d">˂</span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:115%"><span iransans="" style="font-family:"><span style="color:#0d0d0d">P</span></span></span></span><span lang="FA" style="font-size:10.0pt"><span style="line-height:115%"><span iransans="" style="font-family:"><span style="color:#0d0d0d">) در مقایسه با گروه تخریب مشاهده شد.<b> </b></span></span></span></span></span></span></span></span></span><br> &nbsp; <div style="text-align: justify;"><span style="font-size:12pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:9.0pt"><span style="line-height:115%"><span iransans="" style="font-family:"><span style="color:#002060">Background:</span></span></span></span></b> <span style="font-size:9.0pt"><span style="line-height:115%"><span iransans="" style="font-family:"><span style="color:#404040">Alzheimer&#39;s disease (AD) is a neurodegenerative disorder with severe cognitive impairments. Taking into account the role of folic acid on improving cognitive functions in some disease models, the present study aimed to examine the effect of folic acid on spatial learning and memory impairment induced by bilateral electrical lesion of nucleus basalis magnocellularis (NBM) in Alzheimer&rsquo;s disease model of adult male rats.</span></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:9.0pt"><span style="line-height:115%"><span iransans="" style="font-family:"><span style="color:#002060">Materials and Methods:</span></span></span></span></b> <span lang="EN" style="font-size:9.0pt"><span style="line-height:115%"><span iransans="" style="font-family:"><span style="color:#404040">In this experimental study, </span></span></span></span><span style="font-size:9.0pt"><span style="line-height:115%"><span iransans="" style="font-family:"><span style="color:#404040">49 adult male Wistar rats</span></span></span></span><span lang="EN" style="font-size:9.0pt"><span style="line-height:115%"><span iransans="" style="font-family:"><span style="color:#404040"> were randomly divided into </span></span></span></span><span style="font-size:9.0pt"><span style="line-height:115%"><span iransans="" style="font-family:"><span style="color:#404040">seven</span></span></span></span><span lang="EN" style="font-size:9.0pt"><span style="line-height:115%"><span iransans="" style="font-family:"><span style="color:#404040"> groups: Negative </span></span></span></span><span style="font-size:9.0pt"><span style="line-height:115%"><span iransans="" style="font-family:"><span style="color:#404040">control, NBM lesion (bilateral electric lesion of NBM), sham lesion (electrode entry into NBM without induction of electric current), vehicle (lesion+saline), and lesion+folic acid (FA 5, 10, 15 mg/kg). In the treated groups, intraperitoneal injection of folic acid or saline was performed for one week, 30 minutes after the NBM lesion. Then, they were trained for five days by Y-shaped maze. Twenty-five days after the training, a memory recall test was performed to evaluate long-term memory.</span></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:9.0pt"><span style="line-height:115%"><span iransans="" style="font-family:"><span style="color:#002060">Results:</span></span></span></span></b> <span style="font-size:9.0pt"><span style="line-height:115%"><span iransans="" style="font-family:"><span style="color:#404040">NBM bilateral lesion decreased learning and spatial memory compared to the negative and sham control groups (P</span></span></span></span><span style="font-size:9.0pt"><span style="line-height:115%"><span arial="" style="font-family:"><span style="color:#404040">˂</span></span></span></span><span style="font-size:9.0pt"><span style="line-height:115%"><span iransans="" style="font-family:"><span style="color:#404040">0.001). There was a significant increase in learning and spatial memory in the lesion+FA 10 mg/kg (p<0.05) and lesion+FA 15 mg/kg (P<0.01) groups compared to the lesion group.</span></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:9.0pt"><span style="line-height:115%"><span iransans="" style="font-family:"><span style="color:#002060">Conclusion:</span></span></span></span></b> <span style="font-size:9.0pt"><span style="line-height:115%"><span iransans="" style="font-family:"><span style="color:#404040">The results of this study show that folic acid improves learning and spatial memory in Alzheimer&#39;s disease models with bilateral electrical lesions of NBM in adult male rats.</span></span></span></span></span></span></span></div> اسید‌فولیک, یادگیری, حافظه فضایی, هسته قاعده‌ای مگنوسلولاریس, بیماری آلزایمر Folic Acid, learning, spatial memory, Nucleusbasalisof mag-nocellularis, Alzheimer’s disease 138 147 http://ismj.bpums.ac.ir/browse.php?a_code=A-10-1115-18&slc_lang=fa&sid=1 Nastaran Zamani نسترن زمانی na_zamani2000@pnu.ac.ir 5700319475328460016329 5700319475328460016329 Yes Department of Biology, School of Science, Payame Noor University, Tehran, Iran گروه زیست‌شناسی، دانشکده علوم، دانشگاه پیام نور، تهران، ایران