fa ارتباط بین سطح سرمی لیپوپروتئین با چگالی بالا (HDL) و پایین (LDL) با شدت بیماری پارکینسون در مدل حیوانی فیزیولوژی Physiology پژوهشي Original <div style="text-align: justify;"><span style="font-size:12pt"><span style="line-height:117%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:10.0pt"><span style="line-height:117%"><span iransans="" style="font-family:"><span style="color:#1f4e79">زمینه:</span></span></span></span></b> <span lang="FA" style="font-size:10.0pt"><span style="line-height:117%"><span iransans="" style="font-family:"><span style="color:#0d0d0d">بیماری پارکینسون (</span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:117%"><span iransans="" style="font-family:"><span style="color:#0d0d0d">PD</span></span></span></span><span lang="FA" style="font-size:10.0pt"><span style="line-height:117%"><span iransans="" style="font-family:"><span style="color:#0d0d0d">) دومین بیماری شایع نورودژنراتیو است. استرس اکسیداتیو و دیس‌لیپیدمی ممکن است نقش کلیدی در پاتوژنز و پیشرفت </span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:117%"><span iransans="" style="font-family:"><span style="color:#0d0d0d">PD</span></span></span></span><span lang="FA" style="font-size:10.0pt"><span style="line-height:117%"><span iransans="" style="font-family:"><span style="color:#0d0d0d"> داشته باشند. در این مطالعه تجربی، ارتباط بین سطح سرمی </span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:117%"><span iransans="" style="font-family:"><span style="color:#0d0d0d">HDL</span></span></span></span><span lang="FA" style="font-size:10.0pt"><span style="line-height:117%"><span iransans="" style="font-family:"><span style="color:#0d0d0d"> و </span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:117%"><span iransans="" style="font-family:"><span style="color:#0d0d0d">LDL</span></span></span></span><span lang="FA" style="font-size:10.0pt"><span style="line-height:117%"><span iransans="" style="font-family:"><span style="color:#0d0d0d"> با شدت بیماری پارکینسون در مدل حیوانی بررسی گردید.</span></span></span></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="line-height:117%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:10.0pt"><span style="line-height:117%"><span iransans="" style="font-family:"><span style="color:#1f4e79">مواد و روش‌ها:</span></span></span></span></b><b> </b><span lang="FA" style="font-size:10.0pt"><span style="line-height:117%"><span iransans="" style="font-family:"><span style="color:#0d0d0d"><span style="letter-spacing:-.1pt">موش‌ها به سه گروه کنترل، شم (تزریق حلال) و پارکینسونی (تزریق 6-هیدروکسی‌دوپامین، 4 میکروگرم/ میکرولیتر، حجم 10 میکرولیتر به استریاتوم) تقسیم شدند. بروز و شدت علائم پارکینسون قبل و پس از جراحی با آزمون رفتاری چرخش القاء شده با آپومورفین ارزیابی گردید. خونگیری یک بار قبل از جراحی و دو نوبت (هفته‌های سوم و دهم) پس از جراحی انجام شد و سطح سرمی </span></span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:117%"><span iransans="" style="font-family:"><span style="color:#0d0d0d"><span style="letter-spacing:-.1pt">HDL</span></span></span></span></span><span lang="FA" style="font-size:10.0pt"><span style="line-height:117%"><span iransans="" style="font-family:"><span style="color:#0d0d0d"><span style="letter-spacing:-.1pt"> و </span></span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:117%"><span iransans="" style="font-family:"><span style="color:#0d0d0d"><span style="letter-spacing:-.1pt">LDL</span></span></span></span></span><span lang="FA" style="font-size:10.0pt"><span style="line-height:117%"><span iransans="" style="font-family:"><span style="color:#0d0d0d"><span style="letter-spacing:-.1pt"> به روش کالریمتریک اندازه‌گیری شد.</span></span></span></span></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="line-height:117%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:10.0pt"><span style="line-height:117%"><span iransans="" style="font-family:"><span style="color:#1f4e79">یافته‌ها:</span></span></span></span></b> <span lang="FA" style="font-size:10.0pt"><span style="line-height:117%"><span iransans="" style="font-family:"><span style="color:#0d0d0d">سطح سرمی </span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:117%"><span iransans="" style="font-family:"><span style="color:#0d0d0d">LDL</span></span></span></span><span lang="FA" style="font-size:10.0pt"><span style="line-height:117%"><span iransans="" style="font-family:"><span style="color:#0d0d0d"> و </span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:117%"><span iransans="" style="font-family:"><span style="color:#0d0d0d">HDL</span></span></span></span><span lang="FA" style="font-size:10.0pt"><span style="line-height:117%"><span iransans="" style="font-family:"><span style="color:#0d0d0d"> قبل از جراحی به ترتیب1/3&plusmn;22/5 و 1/1&plusmn;31/6 میلی‌گرم/ دسی‌لیتر بود. در گروه دریافت‌کننده سم، سطح </span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:117%"><span iransans="" style="font-family:"><span style="color:#0d0d0d">HDL</span></span></span></span><span lang="FA" style="font-size:10.0pt"><span style="line-height:117%"><span iransans="" style="font-family:"><span style="color:#0d0d0d"> در هفته‌های سوم و دهم به‌طور معناداری کاهش و سطح </span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:117%"><span iransans="" style="font-family:"><span style="color:#0d0d0d">LDL</span></span></span></span><span lang="FA" style="font-size:10.0pt"><span style="line-height:117%"><span iransans="" style="font-family:"><span style="color:#0d0d0d"> به‌طور معناداری افزایش یافت (0/01></span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:117%"><span iransans="" style="font-family:"><span style="color:#0d0d0d">P</span></span></span></span><span lang="FA" style="font-size:10.0pt"><span style="line-height:117%"><span iransans="" style="font-family:"><span style="color:#0d0d0d">). کاهش </span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:117%"><span iransans="" style="font-family:"><span style="color:#0d0d0d">HDL</span></span></span></span><span lang="FA" style="font-size:10.0pt"><span style="line-height:117%"><span iransans="" style="font-family:"><span style="color:#0d0d0d"> در موش‌های با علائم شدید بیشتر از علائم خفیف بود، در حالی که افزایش </span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:117%"><span iransans="" style="font-family:"><span style="color:#0d0d0d">LDL</span></span></span></span><span lang="FA" style="font-size:10.0pt"><span style="line-height:117%"><span iransans="" style="font-family:"><span style="color:#0d0d0d"> فقط در گروه با علائم شدید مشاهده شد.</span></span></span></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="line-height:117%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:10.0pt"><span style="line-height:117%"><span iransans="" style="font-family:"><span style="color:#1f4e79">نتیجه‌گیری:</span></span></span></span></b> <span lang="FA" style="font-size:10.0pt"><span style="line-height:117%"><span iransans="" style="font-family:"><span style="color:#0d0d0d">القای پارکینسون با سم 6-هیدروکسی‌‌دوپامین در موش صحرایی با تغییرات سطح سرمی </span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:117%"><span iransans="" style="font-family:"><span style="color:#0d0d0d">LDL</span></span></span></span><span lang="FA" style="font-size:10.0pt"><span style="line-height:117%"><span iransans="" style="font-family:"><span style="color:#0d0d0d"> و </span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:117%"><span iransans="" style="font-family:"><span style="color:#0d0d0d">HDL</span></span></span></span><span lang="FA" style="font-size:10.0pt"><span style="line-height:117%"><span iransans="" style="font-family:"><span style="color:#0d0d0d"> همراه است و این تغییرات به شدت علائم رفتاری وابسته می‌باشد.</span></span></span></span></span></span></span></span></span></div> <div style="text-align: justify;"><span style="font-size:12pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:9.0pt"><span style="line-height:115%"><span iransans="" style="font-family:"><span style="color:#002060">Background:</span></span></span></span></b><span style="font-size:9.0pt"><span style="line-height:115%"><span iransans="" style="font-family:"><span style="color:#404040"> Parkinson&#39;s disease (PD) is the second most common neurodegenerative disease. Oxidative stress and dyslipidemia may play a key role in the pathogenesis and progression of PD. In this experimental study, the relationship of serum levels of high-density lipoprotein (HDL) and low-density lipoprotein (LDL) with the severity of Parkinson&#39;s disease was investigated in an animal model.</span></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:9.0pt"><span style="line-height:115%"><span iransans="" style="font-family:"><span style="color:#002060">Materials and Methods:</span></span></span></span></b><span style="font-size:9.0pt"><span style="line-height:115%"><span iransans="" style="font-family:"><span style="color:#404040"> Rats were divided into three groups: control, sham (vehicle injection) and parkinsonian (injection of 6-hydroxydopamine, 4 </span></span></span></span><span style="font-size:9.0pt"><span style="line-height:115%"><span calibri="" style="font-family:"><span style="color:#404040">&mu;</span></span></span></span><span style="font-size:9.0pt"><span style="line-height:115%"><span iransans="" style="font-family:"><span style="color:#404040">g/</span></span></span></span><span style="font-size:9.0pt"><span style="line-height:115%"><span calibri="" style="font-family:"><span style="color:#404040">&mu;</span></span></span></span><span style="font-size:9.0pt"><span style="line-height:115%"><span iransans="" style="font-family:"><span style="color:#404040">l, and volume 10 </span></span></span></span><span style="font-size:9.0pt"><span style="line-height:115%"><span calibri="" style="font-family:"><span style="color:#404040">&mu;</span></span></span></span><span style="font-size:9.0pt"><span style="line-height:115%"><span iransans="" style="font-family:"><span style="color:#404040">l into the striatum). The incidence and severity of Parkinson&#39;s symptoms were assessed before and after surgery by apomorphine-induced rotation behavioral test. Blood sampling was performed once before surgery and twice after surgery (weeks 3 and 10), and serum HDL and LDL levels were measured by colorimetric methods.</span></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:9.0pt"><span style="line-height:115%"><span iransans="" style="font-family:"><span style="color:#002060">Results: </span></span></span></span></b><span style="font-size:9.0pt"><span style="line-height:115%"><span iransans="" style="font-family:"><span style="color:#404040">Pre-surgery serum LDL and HDL levels were 22.5&plusmn;1.3 and 31.6&plusmn;1.1 mg/dl, respectively. In the toxin-receiving group, HDL levels decreased significantly on weeks 3 and 10, while LDL levels increased significantly (P<0.01). The decrease in HDL was greater in rats with severe symptoms than in those with mild symptoms, whereas the increase in LDL was observed only in the severe symptom group.</span></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:9.0pt"><span style="line-height:115%"><span iransans="" style="font-family:"><span style="color:#002060"><span style="letter-spacing:.2pt">Conclusion:</span></span></span></span></span></b><span style="font-size:9.0pt"><span style="line-height:115%"><span iransans="" style="font-family:"><span style="color:#404040"><span style="letter-spacing:.2pt"> Induction of Parkinson&#39;s disease by 6-hydroxydopamine toxin in rats is accompanied by changes in serum LDL and HDL levels, and these changes depend on the severity of behavioral symptoms.</span></span></span></span></span></span></span></span></div> بیماری پارکینسون, HDL, LDL, 6-هیدروکسی‌دوپامین Parkinson's disease, HDL, LDL, 6-Hydroxydopamine 66 78 http://ismj.bpums.ac.ir/browse.php?a_code=A-10-1115-88&slc_lang=fa&sid=1 Hashem Haghdoost Yazdi هاشم حق دوست یزدی 5700319475328460020209 5700319475328460020209 No Department of Physiology, School of MedIcne, Qazvin University of Medical Sciences, Qazvin, Iran گروه فیزیولوژی، دانشکده پزشکی، دانشگاه علوم پزشکی قزوین، قزوین، ایران Mohammad Sofiabadi محمد صوفی آبادی msofiabadi@qums.ac.ir 5700319475328460020210 5700319475328460020210 Yes Department of Physiology, School of MedIcne, Qazvin University of Medical Sciences, Qazvin, Iran گروه فیزیولوژی، دانشکده پزشکی، دانشگاه علوم پزشکی قزوین، قزوین، ایران Hossein Khadem Haghighian حسین خادم حقیقیان 5700319475328460020211 5700319475328460020211 No Department of Nutrition, School of Health, Qazvin University of Medical Sciences, Qazvin, Iran گروه تغذیه، دانشکده بهداشت، دانشگاه علوم پزشکی قزوین، قزوین، ایران Maryam Yaghoubi مریم یعقوبی 5700319475328460020212 5700319475328460020212 No Department of Physiology, School of MedIcne, Qazvin University of Medical Sciences, Qazvin, Iran گروه فیزیولوژی، دانشکده پزشکی، دانشگاه علوم پزشکی قزوین، قزوین، ایران