per
Bushehr University of Medical Sciences
Iranian South Medical Journal
1735-4374
1735-6954
2017-01
19
6
931
939
article
Association of HSP70-2 Gene 1267A/G Polymorphism With Cataract Incidence Among Guilan Population
Zivar Salehi
geneticzs@yahoo.co.uk
1
Zahra Gholaminia
2
Mohammad Reza Panjtanpanah
3
Department of Biology, School of Sciences, University of Guilan, Rasht, Iran
Department of Biology, School of Sciences, University of Guilan, Rasht, Iran
Department of Ophthalmology, School of medicine, Guilan University of Medical Sciences, Rasht, Iran
Background: Cataract is a visible opacity of the eye lens and it is the main reason of reversible blindness in the world. Oxidative stress is known as a major factor in cataract formation HSP70-2 protein is a molecular chaperone which is essential for cell survival in stress conditions. HSP70-2 gene is located in the human leukocyte antigen class ΙΙΙ region. This gene encodes an inducible protein. One of the common polymorphism of HSP70-2 is 1267A/G which is located in coding region. The aim of this study was to analysis of 1267A/G polymorphism of hsp70-2 gene in cataract patients.
Material and Methods: This case-control study included 118 cataract patients and 122 healthy people as a control groups. Genomic DNA was extracted from peripheral blood leukocytes and genotyping determination was performed by the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. Statistical analysis was performed by using the MedCalc software (Version 12.1).
Results: The frequency of G allele was significantly higher in patients than the controls, (0.36 and 0.24, respectively). A higher frequency of the GG genotype of the HSP70-2 1267A/G polymorphism was found in the patients compared with controls (21.19% and 8.20%, respectively). The patients carrying the GG genotype were 3.2-fold at a higher risk of cataract compared with AA genotype (P=0.005).
Conclusion: The finding of this study suggested that the HSP70-2 1267A/G may affect the susceptibility to cataract in the studied population. Anyway the randomized multicenter studies with greater sample size still need to confirmed our results.
http://ismj.bpums.ac.ir/article-1-841-en.pdf
Cataract
gene polymorphism
HSP70-2 gene
oxidative stress
per
Bushehr University of Medical Sciences
Iranian South Medical Journal
1735-4374
1735-6954
2017-01
19
6
940
950
article
Methylation Status of NANOG1, RASSF1A, SFN, CASP8, WIF1,CTSL2 Genes in Iranian Breast Cancer Patients
Ali Motevalizadeh Ardekani
Ardekani@nigeb.ac.ir
1
Mahsa Shirani
2
Ahmad Hashemi
3
Zohre Basiri
4
Yazdan Rahmati
5
Novin Nikbakhsh
6
Sahar Edrisi
7
Shahla Mohammadganji
8
Department of Medical Biotechnology, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran
Department of Medical Biotechnology, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran
Department of Medical Biotechnology, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran
Department of Medical Biotechnology, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran
Department of Medical Biotechnology, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran
Cancer Research Center, Babol University of Medical Sciences, Babol, Iran
Cancer Research Center, Babol University of Medical Sciences, Babol, Iran
Department of Medical Biotechnology, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran
Background: Mammographic screening to diagnose the breast cancer showe false-negative and false-positive results in young women and therefore a non-invasive and low cost method is needed, to diagnose the breast cancer in the early stages. DNA methylation changes are the most common molecular changes in human cancers and including breast cancer. Therefore, The pattern of tissues methylation can be used in the early diagnosis of cancer. Also similar methylation patterns found in tumors and in plasma shows potential application of molecular detection of breast cancer, based on blood. The aim of this study was to assess the promoter methylation for the clinical diagnosis of breast cancer.
Material and Methods: To examine the promoter methylation 21 tumor tissues and 21 normal tissues have been studied for clinical diagnosis of breast cancer. 6 gene methylation status (NANOG1, RASSF1A, SFN, CASP8, WIF1 and CTSL2) was analyzed by methylation specific PCR (MS-PCR).
Results: The results show that NANOG gene was methylated in 94.7% of tumor specimen and 100% of normal specimen, RASSF1A gene was methylated in 9.5% of tumor specimen and 0% of normal specimen, SFN gene was methylated in 14.3% of tumor specimen and 27.8% of normal specimen, CASP8 gene was methylated in 30% of tumor specimen and 23.5% of normal specimen, WIF1 gene was methylated in 80% of tumor specimen and 27.8% of normal specimen, CTSL2 gene was methylated in 28.6% of tumor specimen and 23.5 % of normal specimen were methylated. Data analysis did not show a significant relationship between these results. (P >0.05).
Conclusion: The results of this study demonstrate that 6 gene methylation status was not enough to differentiate between the cancer and normal groups. This study demonstrates the methodological problems (MS PCR) which was used to assess the methylation markers to evaluate the methylation status as diagnostic biomarkers.
http://ismj.bpums.ac.ir/article-1-842-en.pdf
DNA methylation
breast cancer
MS-PCR
NANOG1
RASSF1A
SFN
CASP8
WIF1
CTSL2 genes
per
Bushehr University of Medical Sciences
Iranian South Medical Journal
1735-4374
1735-6954
2017-01
19
6
951
961
article
Nano Packaged Diblock and Curcumin: a New Approach Inorder To Drug Resistance in Breast Cancer
Samira Hajigholami
1
Ziba Vaise Malekshahi
zvmalekshahi@yahoo.com
2
Genetic group, School of biological sciences, Tarbiat Modares University Tehran, Iran
Medical Biotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran
Background: Breast cancer is one of the causes of mortality in the world. Half of patients with metastatic extend resistance to Tamoxifen and almost all patients eventually become resistant to tamoxifen. Inorder to counteract with the negative effects of tamoxifen, this drug have been placed in combination with the curcumin. Anti-cancer substance which is less toxic than Tamoxifen. In this study, Diblock (Dendrosome) Nano polymer was used in order to tamoxifen packaging along with curcumin. Anti-cancer efficacy of the obtained compound (Diblock, Tamoxifen and Curcumin) was evaluated in Tamoxifen-resistant (TR)
MCF-7 cells and Fibroblast cells.
Material and Methods: MTT Assay was used to evaluate anti-proliferation effect and drug toxicity.Flow cytometry and Annexin-V-FLUOS were used inorder to assay anti-proliferation effect and induction of apoptosis, respectively.
Results: Nano-compound has less toxicity effects on normal cells compared with Tamoxifen and increased apoptosis activity and decreased proliferation in MCF-7 cells which are resistant to tamoxifen. Curcumin and Tamoxifen have more apoptotic potential than Tamoxifen alone, Nano-Tamoxifen or Nano-Curcumin.
Conclusion: Results of this study showed that changes in Tamoxifen by curcumin polymeric nanocarrier help to more effective treatment of breast cancer.
http://ismj.bpums.ac.ir/article-1-843-en.pdf
Curcumin
Tamoxifen
Polymer Nano-carrier
Breast Cancer
per
Bushehr University of Medical Sciences
Iranian South Medical Journal
1735-4374
1735-6954
2017-01
19
6
962
971
article
Association Between Aeroallergens and Allergic Diseases Based on Skin Prick Test in Bushehr Province
Ali Moradi
1
Afshin Shirkani
2
Rahim Tahmasebi
3
Abdolmajid Omrani
4
Shokrollah Farrokhi
sh.farokhi@bpums.ac.ir
5
The Persian Gulf Nuclear Medicine Research Center, The Persian Gulf Biomedical Research Institute, Bushehr University of Medical Sciences, Bushehr, Iran
Department of Immunology, Asthma and Allergy, The Persian Gulf Tropical Medicine Research Center, The Persian Gulf Biomedical Research Institute, Bushehr University of Medical Sciences, Bushehr, Iran
Department of Biostatistics, School of Public Health, Bushehr University of Medical Sciences, Bushehr, Iran
Department of Pediatrics, School of Medicine, Bushehr University of Medical Sciences, Bushehr, Iran
The Persian Gulf Nuclear Medicine Research Center, The Persian Gulf Biomedical Research Institute, Bushehr University of Medical Sciences, Bushehr, Iran<br> Department of Immunology, Asthma and Allergy, The Persian Gulf Tropical Medicine Research Center, The Persian Gulf Biomedical Research Institute, Bushehr University of Medical Sciences, Bushehr, Iran
Background: The prevalence of allergic diseases is growing in the world and Iran. Allergens play an important role in creating these diseases. Since inhalation allergens are an important part of these allergens the aim of this study was to assess the prevalence of aeroallergens and their association with allergic diseases based on skin prick test (SPT).
Material and Methods: In this study, the frequency of these allergens were examined by using 22 aeroallergens and based on Skin Prick Test in 1100 patients with allergic diseases who referred to Shohadaye- Khalije- Fars university hospital.
Results: The reaction severity of SPT and the severity of disease associated with inhaled allergens with Russian thistle (21.1%, P꞊0.001), Chenopodium album pollen (14%, P꞊0.001) and Dermatophagoid Pteronyssinus (9%, P꞊0.001) respectively. Also, in these patients, the frequency of inhalation allergens were House Dust Mite (HDM) (69%), feather (60.8%), Russian this tle (59.9%) pollen and Alternaria mold (59.6%) respectively.
Conclusion: This study indicated that outdoors inhaled allergens such as Russian thistle and Chenopodium album pollen have the most allergenicity in patients with allergic and asthma in Bushehr province, Meanwhile, the prevalence of indoor aeroallergens such as HDM, feather and mold were high in these patients.
http://ismj.bpums.ac.ir/article-1-844-en.pdf
Allergic Diseases
Asthma
Inhaler Aeroallergens
Skin Prick Test
per
Bushehr University of Medical Sciences
Iranian South Medical Journal
1735-4374
1735-6954
2017-01
19
6
972
981
article
Study of Prevalence and Risk Factors of Asthma and Allergic Diseases Among School Children (6-7 and 13-14 years) Based on ISAAC Protocol in Jam City, Bushehr Province in 2014
Tahere Assadi
1
Mohammadkazem Gheybi
2
Afshin Shirkani
3
Ali Movahed
4
Shaghaeagh Khoddami
5
Ahmad Ashourinejad
6
Shokroola Farrokhi
sh.farokhi@bpums.ac.ir
7
The Persian Gulf Nuclear Medicine Research Center, The Persian Gulf Biomedical Sciences Research Institute, Bushehr University of Medical Sciences, Bushehr, Iran
Department of Immunology, Asthma and Allergy, The Persian Gulf Tropical Medicine Research Center, The Persian Gulf Biomedical Sciences Research Institute, Bushehr University of Medical Sciences, Bushehr, Iran
Department of Immunology, Asthma and Allergy, The Persian Gulf Tropical Medicine Research Center, The Persian Gulf Biomedical Sciences Research Institute, Bushehr University of Medical Sciences, Bushehr, Iran
Depatmant of Biochemistry, The Persian Gulf Marine Biotechnology Research Center, The Persian Gulf Biomedical Sciences Research Institute, Bushehr University of Medical Sciences, Bushehr, Iran
Department of Immunology, Asthma and Allergy, The Persian Gulf Tropical Medicine Research Center, The Persian Gulf Biomedical Sciences Research Institute, Bushehr University of Medical Sciences, Bushehr, Iran
Department of Immunology, Asthma and Allergy, The Persian Gulf Tropical Medicine Research Center, The Persian Gulf Biomedical Sciences Research Institute, Bushehr University of Medical Sciences, Bushehr, Iran
The Persian Gulf Nuclear Medicine Research Center, The Persian Gulf Biomedical Sciences Research Institute, Bushehr University of Medical Sciences, Bushehr, Iran<br> Department of Immunology, Asthma and Allergy, The Persian Gulf Tropical Medicine Research Center, The Persian Gulf Biomedical Sciences Research Institute, Bushehr University of Medical Sciences, Bushehr, Iran
Background: Asthma and allergic diseases are raised as a major health problem in the world. The prevalence of these diseases is on the rise specifically in the world and Iran. Accordingly, the aim of this study was to assess the prevalence of these diseases in Jam City, from environs of Bushehr Province.
Material and Methods: this study was performed on 516 schoolchildren aged 6-7 years and 569 schoolchildren aged 13-14 years in schools of this city based on a standard questionnaire of allergic conditions and asthma (ISAAC) phase 1 and 3, which mention to the prevalence and risk factors for these diseases in 2014.
Results: in prevalence of atopic eczema, allergic rhinitis and asthma among 6-7year old schoolchildren were 13.8 %, 20% and 3.9%, respectively, While, the prevalence of these diseases among 13-14 year old schoolchildren were 19.5%, 19.2% and 6.7%, respectively. There were a significant association in each group, between affliction rate to asthmaand its association with rhinitis and eczema (P<0.05). Also, there were a significant association between the prevalence of these diseases and the presence of risk factors such as exposure to cigarette smoke, pet care and use of gas as a fuel at home. (P<0.05).
Conclusion: the prevalence of asthma and allergic diseases was high in Jam city and this increase is associated to risk factors such as exposure to cigarette smoke, pet care and use of gas as a fuel at home.
http://ismj.bpums.ac.ir/article-1-849-en.pdf
Prevalence
Asthma
atopic eczema
allergic rhinitis
ISAAC
per
Bushehr University of Medical Sciences
Iranian South Medical Journal
1735-4374
1735-6954
2017-01
19
6
982
988
article
To Determine the Frequency of Bacillus cereus in Powdered Milk Infant Formula Consuming in Neonatal Intensive Care Unit (NICU) in Tehran Hospitals in 2013-14
Mohammad Mehdi Soltan Dallal
msoltandallal@gmail.com
1
Shirin Nezamabadi
2
Mohammadkazem Sharifi Yazdi
3
Jalal Mardaneh
4
Mehrnaz Taheripoor
5
Food Microbiology Research Center, Tehran University of Medical Sciences, Tehran, Iran<br> Division of Food Microbiology, Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
Division of Food Microbiology, Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
Center for Communicable Disease Control, Ministry of Health and Medical Education, Tehran, Iran<br> Department of Epidemiology and Biostatics, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
Department of Microbiology, School of Medicine, Gonabad University of Medical Sciences, Gonabad, Iran
Food Microbiology Research Center, Tehran University of Medical Sciences, Tehran, Iran
Background: In recent years, changing the infant feeding methods and the growing trend of use powdered infant formula (PIF) has raised concern about quality and health assessment among them. These products are contaminated with various pathogenic bacteria such as Bacillus cereus which the presence of this bacteria in PIF is important because of consumer age group and virulence of this bacteria in PIF. The aim of this study was to determine the frequency of Bacillus cereus in powdered milk infant formula consuming in neonatal intensive care unit (NICU) in Tehran hospitals in 2013-14.
Material and Methods: In this cross-sectional study, 125 samples of powdered infant formula milk which were used in Neonatal Intensive Care Unit (NICU) were surveyed during 8 month in 2014. Isolation and identification of microorganisms (including Bacillus cereus) were carried out according to FDA standard protocol (FDA method) on B. cereus selective agar (MYP Agar).
Results: The results of present study showed that of 125 samples from of consumable powdered infant formula milk, 84 (67.2%) samples were contaminated with B.cereus and also 18 (14.4%) samples were contaminated by more than one B.cereus species.
Conclusion: As regards pasteurization process is not effective on the spore of B.cereus., The spores of these bacteria can remain in PIF and can cause food poisoning in infants. For this purpose, more attention to quality control of production units and imported powder milk is recommended in Iranian infant foods.
http://ismj.bpums.ac.ir/article-1-846-en.pdf
Powdered infant formula milk
Bacillus cereus
Neonatal
MYP media
per
Bushehr University of Medical Sciences
Iranian South Medical Journal
1735-4374
1735-6954
2017-01
19
6
989
1004
article
The Challenge of Adherence From Treatment-Care Regimens Among Patients With Chronic Diseases: A Qualitative Study
Hakemeh Vahedparast
1
Esae Mohammadi
mohamade@modares.ac.ir
2
Fazllolah Ahmadi
3
Department of Nursing, School of Nursing and Midwifery, Bushehr University of Medical Sciences, Bushehr, Iran<br> Department of Nursing, School of Medical Sciences, Tarbiat Modares University Tehran, Iran
Department of Nursing, School of Medical Sciences, Tarbiat Modares University Tehran, Iran
Department of Nursing, School of Medical Sciences, Tarbiat Modares University Tehran, Iran
Background: Adherence from treatment-care regimens is very essential to achieve health promotion and improve quality of life as well as affordable healthcare. Nevertheless, broaden of chronic diseases is still growing in the world because of lack of adherence. Understanding the perceived challenges can facilitate planning to increase adherence. This study aimed to explain the perceived challenges in non-adherence from the prescribed regimen among patients with chronic diseases.
Materials and methods: This qualitative study was done in 2015 by using the conventional content analysis approach. Thirty four participants with the most common chronic diseases were recruited purposively and they were entered in the study. Data was collected through semi-structured interviews and simultaneously with data collection; a comparative analysis was conducted by using the conventional qualitative content analysis method.
Results: Four main themes extracted from the data included: flaw in the possibility of observing the recommended regimensafter devotion for family; frustration in facing the conflict between work and recommended regimens; inability in self-adaption with the recommended regimens; broaden cost of prescribed regimens; and limiting and inductive enclosure of beliefs. These themes indicate the nature of challenges in personal, family, and social dimensions of patients with chronic diseases in terms of adherence with treatment regimen.
Conclusion: The findings show that the perceived challenges has special complexity and diversity. Although they are sometimes internal and personal, some others are external and beyound the will and control of individuals. Therefore, it is necessary that health system policy-makers and planners intervene and plan according to the nature, diversity, and complexity of these perceived barriers in order to facilitate in the adherence of patients process from the treatment care regimens.
http://ismj.bpums.ac.ir/article-1-847-en.pdf
Adherence
Chronic diseases
Perceived Barriers
Treatment regimens
Qualitative Study
per
Bushehr University of Medical Sciences
Iranian South Medical Journal
1735-4374
1735-6954
2017-01
19
6
1005
1010
article
Cystic Hygroma in Newborns, Review Articles and a Case Report
Fatemeh Eghbalian
eghbalian_fa@yahoo.com
1
Pediatric department, Hamadan University of Medical Sciences, Hamedan, Iran
Cystic hygroma which originating from buds lymphatic are a combination of multiple cysts, with benign nature. The incidence of this disease is estimated to be 1 in sixty thousands of live births. 75% of cases occur in the neck and 20 percent is seen in axillary area. There are chromosomal abnormalities in 60% of cases. Cystic hygroma usually covers adjacent neurovascular structures Airway obstruction is the most critical complication, infection and bleeding are on the next level. Complete surgical removal is the selective treatment. The case was a 3-day-old male neonate weighing 4 kg admitted in NICU ward of Beasat hospital due to massive bilateral swelling of the neck and severe respiratory distress. The patient was operated on an emergency basis and cyst was removed. After surgery, the infant was mechanically ventilated for 4 days. There was recurrence following complete resection, the remaining part of the Cystic hygroma was excised at the age of 6 weeks.
http://ismj.bpums.ac.ir/article-1-848-en.pdf
Neonate
Cystic hygroma
Surgery
Disease
per
Bushehr University of Medical Sciences
Iranian South Medical Journal
1735-4374
1735-6954
2017-01
19
6
1011
1021
article
The Correlation of Adiponectin, and Visfatin Serum Levels With Ischemic Heart Disease in Postmenopausal women: A population-Based Study
Hossein Darabi
1
Zahra Behzadi
2
Taliaeh Zahedi
3
Mohammadreza Pourbehi
4
Samad Akbarzadeh
5
Afshin Ostovar
6
Majid Assadi
7
Mohammad Javad Haerinejad
8
Zahra Sanjideh
9
Iraj Nabipour
inabipour@gmail.com
10
The Persian Gulf Tropical Medicine Research Center, The Persian Gulf Biomedical Sciences Research Institute, Bushehr University of Medical Sciences, Bushehr, Iran
The Persian Gulf Tropical Medicine Research Center, The Persian Gulf Biomedical Sciences Research Institute, Bushehr University of Medical Sciences, Bushehr, Iran
The Persian Gulf Tropical Medicine Research Center, The Persian Gulf Biomedical Sciences Research Institute, Bushehr University of Medical Sciences, Bushehr, Iran
The Persian Gulf Tropical Medicine Research Center, The Persian Gulf Biomedical Sciences Research Institute, Bushehr University of Medical Sciences, Bushehr, Iran
The Persian Gulf Tropical Medicine Research Center, The Persian Gulf Biomedical Sciences Research Institute, Bushehr University of Medical Sciences, Bushehr, Iran
The Persian Gulf Tropical Medicine Research Center, The Persian Gulf Biomedical Sciences Research Institute, Bushehr University of Medical Sciences, Bushehr, Iran
The Persian Gulf Nuclear Medicine Research Center, The Persian Gulf Biomedical Sciences Research Institute, Bushehr University of Medical Sciences, Bushehr, Iran
The Persian Gulf Tropical Medicine Research Center, The Persian Gulf Biomedical Sciences Research Institute, Bushehr University of Medical Sciences, Bushehr, Iran
The Persian Gulf Tropical Medicine Research Center, The Persian Gulf Biomedical Sciences Research Institute, Bushehr University of Medical Sciences, Bushehr, Iran
The Persian Gulf Marine Biotechnology Research Center, The Persian Gulf Biomedical Sciences Research Institute, Bushehr University of Medical Sciences, Bushehr, Iran
Background: There is a controversial evidence about adiponectin role in cardiovascular diseases and visfatin role has been suggested in pathogenesis of coronary artery disease.
Material and Methods: A total of 378 postmenopausal women were randomly selected from 13 clusters in Bushehr Port. The prevalence of ischemic heart disease was assessed by using the Minnesota Code with a 12-lead resting electrocardiogram (EKG). Serum adiponectin and visfatin levels were measured by using enzyme-linked immunosorbent assay methods
Results: A total of 159 patients (41.8 percent) were suffering from ischemic heart disease. There was no significant difference between women with ischemic heart disease and healthy postmenopausal women in adiponectin serum level. However, diabetic women with ischemic heart disease, compared with healthy postmenopausal women had a higher level of visfatin (Geometric Mean: 3.05±1.84 ng/ml versus 2.52±1.90 ng/ml, respectively; p=0.023). Multiple logistic regression analysis indicated that higher serum levels of visfatin were significantly associated with a higher prevalence of ischemic heart disease after adjusting for potential confounders, including classical cardiovascular risk factors and circulating hs-CRP levels [odds ratio=3.26, confidence interval (1.14-9.68), p=0.027].
Conclusion: Adiponectin was not correlated with ischemic heart disease among postmenopausal women. But visfatin serum level correlated with ischemic heart disease in postmenopausal non-diabetic women independently from cardiovascular risk factors.
http://ismj.bpums.ac.ir/article-1-850-en.pdf
Menopause
Adiponectin
Visfatin
Ischemic Heart Disease
Adipocytokines