@article{ author = {Seyedesmaeili, Nazanin and Onsory, Khadejeh}, title = {Expression of JAK2 and MicroRNA-216a in Patients with Acute Myeloid Leukemia}, abstract ={Background: Janus Kinase 2 (JAK2) gene functions in JAK/STAT signaling pathway which is one the most important pathways to transfer the effect of growth factors and cytokines to the cell nucleus. Cell growth, differentiation and migration occur after JAK2 is activated. Moreover, overexpression of miR-216a markedly inhibits the JAK2/STAT3 signaling pathway and tumor growth. The purpose of this study was to determine the expression of miR-216a and JAK2 as its target gene in AML patients. Materials and Methods: In this case-control study, the miR-216a and JAK2 expression was investigated in 60 AML patients admitted to Mirza Chochak Khane Jangali Hospital and 34 healthy individuals from Dr. Shariati Hospital, Tehran in 2015-2016. After RNA extraction and cDNA synthesis, the expression of these genes was evaluated using Real-time PCR (ΔΔCT computational). Results: JAK2 gene expression was significantly increased in patients (P=0.0023). Meanwhile, miR-216a expression decreased in healthy individuals (P=0.1361). No significant relationship was observed between variables of age, sex, PLT, HGB, and FAB subtype and expression of the genes (P>0.05). However, a significant relationship was observed between variables of  WBC and Blasts and expression of genes. Conclusion: Expression of miR-216a and JAK2 can therefore be used as a diagnostic agent. They can have a role in prognosis of patients with AML.}, Keywords = {Acute Myeloid Leukemia, JAK2 gene, miR-216a, Real-time PCR}, volume = {21}, Number = {3}, pages = {186-196}, publisher = {Bushehr University of Medical Sciences}, url = {http://ismj.bpums.ac.ir/article-1-926-en.html}, eprint = {http://ismj.bpums.ac.ir/article-1-926-en.pdf}, journal = {Iranian South Medical Journal}, issn = {1735-4374}, eissn = {1735-6954}, year = {2018} } @article{ author = {Eskandary, Azadeh and Moazedi, Ahmad Ali and Zamani, Nastar}, title = {Effect of Lovastatin on Learning and Spatial Memory in Adult Male Rats}, abstract ={Background: Previous studies have shown that statins exert neuroprotective effects. The aim of the present study was to evaluate the effects of lovastatin on learning and memory processes in adult male rats without brain damage. Materials and Methods: In this experimental study, 35 adult male Wistar rats were randomly divided into 5 groups of 7 rats. Group 1, the control, without any injection; groups 2-4, test groups, each group received lovastatin (1, 10 and 20 mg/kg, IP daily for five days) and group 5, vehicle-treated group received the same volume of DMSO 5% as lovastatin vehicle. At the end of the treatment with lovastatin or vehicle, 8-arm radial maze and Y-maze task were used to evaluate spatial memory. Results: The results showed that lovastatin (10, 20 mg/kg) reduced reference and working memory errors and the time elapsed in the 8-arm radial maze task in the acquisition test (P˂0.05) and further reduced these functions in retention testing (P˂0.05) compared to the control group. On the Y maze test, alternation percentage showed a significant increase in 20 mg/kg group compared to the control (P˂0.01). Conclusion: Our results suggest that high doses of lovastatin improve spatial memory although the exact mechanism by which lovastatin affects memory is unclear and requires further investigation.  }, Keywords = {Lovastatin, Learning, Memory, Rat}, volume = {21}, Number = {3}, pages = {197-208}, publisher = {Bushehr University of Medical Sciences}, url = {http://ismj.bpums.ac.ir/article-1-927-en.html}, eprint = {http://ismj.bpums.ac.ir/article-1-927-en.pdf}, journal = {Iranian South Medical Journal}, issn = {1735-4374}, eissn = {1735-6954}, year = {2018} } @article{ author = {Abbasifard, Anahita and Movahed, Ali and Ostovar, Afshin and Bargahi, Afshar and Jaberi, Hajar and Akbarzadeh, Sam}, title = {Serum Irisin Changes Following Endurance Exercise and Caloric Restriction in Obese and Hypertriglyceridemic Rats}, abstract ={Background: The present study aimed to confirm the hypothesis that indicates the role of Irisin as an adipomyokine, in boosting energy consumption and heat generation in brown fat tissue following moderate exercise and calorie restriction. Materials and Methods: In this experimental study, 28 male Wistar rats (180-250g) were divided into four groups: Standard Control (STC), High Fat-high Carbohydrate diet (HFC), Stop High Fat- high Carbohydrate diet (S-HFC) and Exercise (EXE).The first 16 weeks were used to apply a special model (HF-HC diet for long-term) for inducing hypertriglyceridemia and weight gain, followed by five weeks of treatment for both groups (S-HFC and EXE).  S-HFC group diet changed to standard food and the EXE group ran on treadmill for 20 minutes daily for 5 days a week. The other two groups continued the same diet as the first 16 weeks (standard diet for STC and HF-HC diet for HFC). Lipid profile was measured by spectrophotometry and Irisin by ELISA method. Results: The model we used to induce the hypertriglyceridemia (p=0.000) and obese (p=0.001) was significantly successful. Serum triglyceride levels decreased after the treatment (p=0.011) while there were no significant changes in other components of lipid profiles, weight and serum Irisin  concentration. Moreover, the serum concentration of Irisin and triglyceride were not correlated (p=0.693, r=0.071). Conclusion: The result of this study suggested that exercise along with calorie restriction significantly reduced serum triglyceride levels, but did not necessarily affect the weight loss. There was no correlation between the concentration of triglyceride and Irisin.}, Keywords = {Irisin, Obesity, Hypertriglyceridemia, Exercise, Diet}, volume = {21}, Number = {3}, pages = {209-219}, publisher = {Bushehr University of Medical Sciences}, url = {http://ismj.bpums.ac.ir/article-1-928-en.html}, eprint = {http://ismj.bpums.ac.ir/article-1-928-en.pdf}, journal = {Iranian South Medical Journal}, issn = {1735-4374}, eissn = {1735-6954}, year = {2018} } @article{ author = {Abbasi, Farhad and Mirzaei, Parisa and Rastegar, Razieh and Naimee, Zohreh and Sanjideh, Zahra and Vahdat, Katayo}, title = {Prevalence of Measles Antibodies in Medical Students in Bushehr}, abstract ={Background: Measles is one of the most highly communicable of all infectious diseases in healthcare settings, and may transmit to medical personnel. This study was designed to assess measles immunity in a group of healthcare workers (medical students) and its relationship with age, sex and duration of working in hospital. Material and Methods: All medical students of the clinical courses were informed of the study in spring and summer of 2016. After collecting demographic information, 5 ml of blood was drawn from each student. Serum level of measles IgG was measured using IBL-America enzyme-linked immunosorbent assays kits in the laboratory of the Persian Gulf Research Center for Infectious Diseases. Results: A total of 90 students with a mean age of 25±3.2 years were included. All students had a history of one shot of measles vaccination. The positivity rate of measles antibody was found 48.9% (44 cases) while 12.2% (11 cases) had borderline titers and 35 (38.9%) were reported negative. No relationship was observed between positivity of measles antibody and the variables of age, sex and duration of working in hospital. Conclusion: This study demonstrates more than one-third of medical students were at risk of measles, which mandates periodic surveillance and revaccination of susceptible healthcare workers. Furthermore, adequate respiratory precautions should be taken when visiting patients with febrile exanthema and cough.}, Keywords = {Measles, Medical students, Immunity, Vaccination}, volume = {21}, Number = {3}, pages = {220-227}, publisher = {Bushehr University of Medical Sciences}, url = {http://ismj.bpums.ac.ir/article-1-929-en.html}, eprint = {http://ismj.bpums.ac.ir/article-1-929-en.pdf}, journal = {Iranian South Medical Journal}, issn = {1735-4374}, eissn = {1735-6954}, year = {2018} } @article{ author = {Rezaeianpour, Sedegheh and Mosayebnia, Mona and HajiaghaBozorgi, Atefeh and Moghimi, Abolghasem and Balalaie, saeed and Shahhosseini, Soray}, title = {Design, Synthesis and Radiolabeling of Peptide GPRPILE with 18FDG as Fibrin Imaging Agent for Thrombosis Detection}, abstract ={Background: The radionuclide of choice for routine clinical PET imaging is 18-F. As direct fluorination of peptides with 18-F is not possible, indirect methods using fluorinated prosthetic groups have been developed. Due to the availability of 18FDG in most PET centers, there is potential for 18FDG as a fluorinated prosthetic group. In this study, the linear peptide GPRPILE with an aminooxy group was designed, synthesized and radiolabeled with 18FDG as fibrin imaging agent. Material and Methods: Docking studies were conducted done using AutoDock 4.1 and HEX software programs. Aoe-GPRPILE peptide was designed, synthesized through Fmoc method and radiolabeled with 18FDG. The radiochemical purity, stability of radiolabeled and cold peptide in PBS and human plasma was determined using chromatographic methods. The solubility ratio of the radiolabeled peptide in lipid to water (LogP) was determined. Results: Docking and pharmacophore studies using HEX software revealed high affinity of designed peptide to fibrin (E Total=-0.01). The identity and structure of peptide were determined by LC-Mass. Peptide was stable over 24 hr in human plasma and PBS buffer. The optimum conditions of radiolabeling were 0.2 mg peptide, 1 mCi 18FDG, 90°C for 30 min, pH=5. The radiochemical purity was over 95%. The stability of radiolabeled peptide in human plasma for 2 hr was over 95%. The partition coefficient (LogP) was 1.5. Conclusion: 18FDG has a high potential to be used as a prosthetic group for radiolabeling of peptides with 18-F. In this study, peptide Aoe-GPRPILE with aminooxy was synthesized and labeled with 18FDG with high yield and radiochemical purity. Aminooxy is conjugated to peptide sequence as a prosthetic group in the last step with minimal effect on peptide properties and selectively forms stable oxime bond with the aldehyde group of 18FDG.}, Keywords = {Thrombosis, Radiolabeling with 18FDG, Peptide, fibrin}, volume = {21}, Number = {3}, pages = {228-241}, publisher = {Bushehr University of Medical Sciences}, url = {http://ismj.bpums.ac.ir/article-1-930-en.html}, eprint = {http://ismj.bpums.ac.ir/article-1-930-en.pdf}, journal = {Iranian South Medical Journal}, issn = {1735-4374}, eissn = {1735-6954}, year = {2018} } @article{ author = {Faroughi, Fershteh and SalehiBarough, Mehdi}, title = {Effects of Gamma Radiation on Plasma Levels of Cu and Mn in Nuclear Medicine Staff}, abstract ={Background: Copper and manganese play antioxidative roles in cells. Since gamma radiation is frequently used at medical imaging centers, we evaluated possible changes in serum levels of copper and manganese among nuclear medicine staff. Materials and Methods: This cross-sectional study enrolled 30 nuclear medicine staff and 10 individuals as the control group. Blood samples of 20 ml were collected and centrifuged at 4000 rpm for 7 min to separate the sera for measuring copper and manganese through atomic absorption spectroscopy (AAA) with graphite furnace. Results: The mean of copper concentration in the control group (93.85±25.33) was a bit higher than that in the irradiated group (85.6±21.66); however, this difference was not significant (p=0.32). A positive linear correlation was observed between exposure time and the reduced level of copper; however, this relationship was not statistically significant (p=0.05). An insignificant difference was observed in the mean of manganese concentration between the two groups and its relationship with exposure time. Conclusion: Reduced copper level can be considered as one of the possible mechanisms for gamma radiation effect on cells, which may be associated with oxidative damage. However, it needs further studies.  }, Keywords = {Gamma radiation, serum, copper, manganese}, volume = {21}, Number = {3}, pages = {242-252}, publisher = {Bushehr University of Medical Sciences}, url = {http://ismj.bpums.ac.ir/article-1-931-en.html}, eprint = {http://ismj.bpums.ac.ir/article-1-931-en.pdf}, journal = {Iranian South Medical Journal}, issn = {1735-4374}, eissn = {1735-6954}, year = {2018} } @article{ author = {Kashkooli, Faramarz and Khormaei, Farhad and Yousefi, Farideh and Fouladchang, Mahbobeh}, title = {Role of Temperament and Emotional Security in Academic Destructive Behaviors of Secondary School Students}, abstract ={Background: Academic destructive behaviors are influenced by multiple factors such as the temperament and emotional security. This study aimed to describe academic destructive behaviors based on temperament and emotional security. Materials and methods: This correlative study recruited a sample of 503 students, selected through random stratified multi-stage sampling. The  statistical population included all secondary school students in Bushehr city. Data were collected using Temperament Inventory, Emotional Security in the Family System Inventory and Academic Destructive Behaviors Scale. Data were analyzed through the structural equation modeling (SEM) using the Amos 22 software and SPSS 21. Results: Results showed a meaningful relationship between the variable of temperamental traits and both academic destructive behaviors (β= -0.36, P< 0.001) and the emotional security (β=0.52, P< 0.001). There was also a direct meaningful relationship between the variable of emotional security and academic destructive behaviors (β= -0.59, P< 0.001). Besides, the temperamental traits, indirectly and through the mediation of emotional security, showed a significant relationship with academic destructive behaviors (β=- 0.31, P< 0.001). Conclusion: Given the findings of the present study, it is suggested that temperament and emotional security be addressed in planning for education, training and mental health programs.  }, Keywords = {Temperament, Emotional Security, Negligence, Law Breaking, Conflict Disobedience, Dishonesty}, volume = {21}, Number = {3}, pages = {253-266}, publisher = {Bushehr University of Medical Sciences}, url = {http://ismj.bpums.ac.ir/article-1-932-en.html}, eprint = {http://ismj.bpums.ac.ir/article-1-932-en.pdf}, journal = {Iranian South Medical Journal}, issn = {1735-4374}, eissn = {1735-6954}, year = {2018} }