Bushehr University of Medical Sciences
Iranian South Medical Journal
1735-4374
1735-6954
12
2
2009
12
1
Evaluation of HP0605 and HP0971 genes of efflux pumps in Helicobacter pylori resistance to Metronidazole
111
118
FA
Mohammad hasan
Shirazi
mhshirazi@sina.tums.ac.ir
Y
Mohammad Mehdi
Soltan dalal
N
Sara
Haji khani
N
Mohammad reza
Pourmand
Health School
N
Jalil
Fallah Mehrabadi
N
Naser
Moghimi Dariaie
N
Mehrndokht
Sirous
Health School
N
Seied Saeed
Eshraghi
N
Background: The presence of antibiotic resistance has been reported in H.pylori and it is a major cause of treatment failure. Five families of multidrug efflux pumps are defined in bacteria and resistance-nodulation-division (RND) pumps are found mainly in gram negative bacteria. TolC is one of RND pump components and play a critical role in drug resistance. It hasn’t been established that RND family has a role in drug resistance in H.pylori. In this study, we assessed the role of two efflux genes in resistant to metronidazole in H.pylori by evaluation of overexpression TolC genes by RT-PCR method. Methods: In five metronidazole resistant strains of H.pylori, total RNA was extracted. RNA treated with DNase and RNA reverse transcribed to cDNA. Aliquots of the cDNA solution were assayed by RT- PCR for HP0605 and HP0971 genes. The levels of mRNA expression were evaluated by densitometry analysis. Results: All five strains displayed overexpression for HP0605 basis of increased concentration of metronidazole. Three strains showed transcripts for HP0971. One of these had transcripts for HP0971 only in Metronidazole concentration equaled to 16 µg/ml but two strains overexpressed adapt to increase concentration of metronidazole. Conclusion: According to current study, HP0605 and HP0971 genes overexpressed due to increase metronidazole. So, increasing of Metronidazole affects in H.pylori΄s efflux system in transcription level.
H.pylori, efflux pump, TolC, Metronidazole
http://ismj.bpums.ac.ir/article-1-179-en.html
http://ismj.bpums.ac.ir/article-1-179-en.pdf
Bushehr University of Medical Sciences
Iranian South Medical Journal
1735-4374
1735-6954
12
2
2009
12
1
Diagnostic value of “base deficit” in arterial blood gas analysis in comparison with hyperlactatemia as an indicator of tissue hypoperfusion in cardiac surgery
119
125
FA
Rasoul
Azarfarin
Cardiovascular Research Center
N
Iesa
Bile jani
N
Amir abbas
Kianfar
N
Mousa
Miri nejad
N
Azin
Alizadeh asl
alizadeasl@yahoo.com
Y
Background: Hyperlactatemia is a well known index for tissue hypoperfusion which is not routinely measured in all surgical procedures. The aim of this study was to evaluate the diagnostic value of base deficit in detecting hyperlactatemia and also predicting its postoperative complications in cardiac surgery.
Methods: In a prospective observational study, 487 patients who underwent elective coronary artery bypass grafting or valve replacement surgery were evaluated. The serum lactate level and arterial blood gas (ABG) tests were measured simultaneously 3-5 times during operation and every six hours until 24h after operation. Hemodynamic changes during the operation, and also postoperative major complications consisting cardiac, pulmonary, neurologic, renal, infectious complications and death were recorded. Criterion for hyperlactatemia was considerated serum lactate≥3 mg/dl and for metabolic acidosis, base deficit>5 mmol/L.Results: Intraoperative frequency of "at least one episode of" base deficit>5 mmol/L was 41.3% and also hyperlactatemia was 56.3%. There were sensitivity (48%), specificity (68%) and PPV (66%) of base deficit>5 mmol/L in diagnosis of hyperlactatemia, considering pre-test probability (56.3%) of hyperlactatemia and calculating LR=1.49 for "base deficit>5 mmol/L ", the post-test probability of hyperlactatemia was estimated 70%. Considering frequency (pre-test probability) of 26.5% for postoperative complications and calculating LR=1.40 for both base deficit>5 mmol/L and hyperlactatemia, the post-test probability of postoperative complications was estimated 40% (in both tests). Conclusions: Because of importance of major postoperative complications, 40% probability of these events by observing base deficit>5 mmol/L or lactate≥3 mg/dl can be sufficiently alert the anesthesiologist to do proper interventions.
metabolic acidosis, hyperlactatemia, cardiac surgery, diagnostic value
http://ismj.bpums.ac.ir/article-1-180-en.html
http://ismj.bpums.ac.ir/article-1-180-en.pdf
Bushehr University of Medical Sciences
Iranian South Medical Journal
1735-4374
1735-6954
12
2
2009
12
1
Metabolic syndrome among professional bus and truck drivers in Kashan, 2008
126
132
FA
Hamidreza
Saberi
hamisa2002@yahoo.com
Y
Alireza
Moraveji
N
Karim
Parastouie
N
Background: The literature data and our own studies show that in drivers of transport vehicles, work-related risk factors for arterial hypertension, ischemic heart disease, and metabolic syndrome are largely intensified. These involve occupational risk factors as well as classic ones, such as obesity, limited physical activity, shift work, or tobacco smoking. However, studies published over the past two decades on the health of bus and truck drivers have received relatively less attention in developing countries. Methods: In a cross-sectional study 429 Kashan City male bus and truck drivers that enter the occupational health service for health examination were enrolled. The study focused on the presence of the metabolic syndrome, as defined by the National Cholesterol Education Program Expert Panel on Detection, Evaluation and Treatment of High Blood Cholesterol in Adults (NCEP ATP III) and statistical tests such as t-test, chi-square test and Pearson’s correlation coefficient were used to analyze the data. Results: The prevalence of metabolic syndrome according to ATP-III was 35.9% mostly at age range 30-39 years. The prevalence of overweight was 41% Obesity, 23.1% hypertension, 42.9% Diabetes, 7% hypertriglyceridemia, 53.4% and low HDL cholesterol, 48.7%. In addition, between diabetes and hypertension and between hypertension and smoking meaningful relation was seen (p=0.006). Conclusion: We observed a high prevalence of metabolic syndrome in these groups to be significantly higher than other groups in Iran. So, Occupation must be considered as a risk factor for impaired health status, leading to temporary or permanent disability to work.
drivers, metabolic syndrome, diabetes, body mass index, blood pressure, obesity
http://ismj.bpums.ac.ir/article-1-181-en.html
http://ismj.bpums.ac.ir/article-1-181-en.pdf
Bushehr University of Medical Sciences
Iranian South Medical Journal
1735-4374
1735-6954
12
2
2009
12
1
Correlation of maternal factors and hemoglobin concentration during pregnancy Shiraz 2006
133
141
FA
Marzieh
Akbarzadeh
akbarzadehmarzieh@yahoo.com
Y
Mani
Razmi
N
Hamidreza
Tabatabaie
N
Leila
Alizadeh
Nursery and midwifery School
N
Background: Anemia in pregnancy is a serious condition, contributing to maternal mortality, morbidity and fetal morbidity and its prevalence varies between 35-100% in developing countries. This investigation is conducted to survey the correlation of maternal factors and the changes in hemoglobin in pregnant women. Method: In this study, 108 healthy pregnant women with gestational age of 10 to 14 weeks, chosen by cluster random sampling were included. The women were followed in three visits: at the end of the first, second and third trimester. In addition, correlation of Hb concentration with maternal factors including BMI, age parity, hyperemesis, gestational age, pregnancy interval and weight gain was investigated. Results: There was no significant correlation between BMI, parity, pregnancy interval, severe nausea and vomiting and also maternal age with hemoglobin level during pregnancy. Moreover, Multiple regression models showed that adequate maternal weight gain (P<0.009) and high hemoglobin (p<0.0001) in the first trimester were positive predictors and late iron supplementation was negative predictor of hemoglobin in pregnancy (P<0.006). Conclusion: Our data demonstrated that adequate maternal weight gain, high hemoglobin in the first trimester and also late iron supplementation could be as predictors in clinical settings in this query.
Pregnancy, Hemoglobin, Maternal factors, anemi, Body mass index (BMI)
http://ismj.bpums.ac.ir/article-1-182-en.html
http://ismj.bpums.ac.ir/article-1-182-en.pdf
Bushehr University of Medical Sciences
Iranian South Medical Journal
1735-4374
1735-6954
12
2
2009
12
1
Study of frequency of bacteria isolated from blood culture and their antibiotic susceptibility pattern in a university hospital in Tehran
142
148
FA
Hoorieh
Saderi
saderih@yahoo.com
Y
Ali akbar
Karimi
N
Marzieh
Loni
N
Introduction: Determining frequency of bacteria, isolated from blood culture and their antibiotic susceptibility patterns, has epidemiological significance and can help in selecting empirical therapy. This study was aimed to assess, the frequency of bacteria isolated from blood culture of patients suspected to bacteremia and their antibiotic susceptibility patterns. Methods: Culture of blood and determination of antibiotic susceptibility was done by standard methods. In this study, a variety of isolated bacteria types, antibiotic susceptibility, as well as age, sex and type of admission of patients were analyzed in a university hospital from 21 March, 2006 to 20 March, 2007. Results: During one year, blood culture was done for 5116 patients and bacteria were isolated in 912 cases (17.8%). Three most frequently groups of bacteria in blood cultures of patients were non-fermentative gram negative bacteria (Pseudomonas and Acintobacter spp), coliforms (Escherichia coli and enterobacter and klebsiella spp.) and coagulase negative staphylococci, respectively, which were isolated in 63.4%, 17.0% and 12.8% of patients, and constituted 93.2% of positive blood cultures. Higher resistance was shown in bacteria isolated from inpatients compare to outpatients. Conclusion: This study showed the influence of age, sex and type of admission (outpatient or inpatient) in a variety of isolated bacteria in blood culture. The result of this study were the same as the other studies in Iran and other countries in respect of the variety of isolated bacteria and antibiotic susceptibility and show increase of antibiotic resistance in these bacteria.
antibiotic susceptibility, bacteremia, blood culture, empirical therapy
http://ismj.bpums.ac.ir/article-1-183-en.html
http://ismj.bpums.ac.ir/article-1-183-en.pdf
Bushehr University of Medical Sciences
Iranian South Medical Journal
1735-4374
1735-6954
12
2
2009
12
1
Review of frequency of abnormal imaging finding in magnetic resonance cholangiopancreatography
149
156
FA
Jamshid
Saberi fard
jsaberifard@yahoo.com
Y
Ali
Radmehr
N
Background: Magnetic resonance cholangiopancreatography (MRCP) has had a significant technical improvement during recent years. This method of imaging can have a broad range of clinical applications in imaging of pancreatic and biliary ducts. Recent studies have shown that magnetic resonance cholangiopancreatography is a comparable and better technique than other techniques such as sonography, CT scan and endoscopic retrograde cholangiopancreatography to assess pancreaticobiliary system. Methods: In this study heavily T2 weighted MR sequence was used to show slow flowing fluid as high signal intensity in bile ducts and main pancreatic duct. We reviewed MRCP imaging findings of 293 patients with pancraticobiliary diseases and analyzed the frequency of different pathologies and disorders during the years 1379 to 1385. Results: We found that stones in adults and also congenital diseases of bile ducts such as choledochal cysts in children were the most frequent pancreaticobiliary problems depicted by MRCP. Conclusion: MRCP is a valuable tool for diagnostic imaging of pancreaticobiliary system and can elegantly show different pathologies noninvasively and accurately.
cholangiopancreatography, magnetic resonance imaging cholangiopancreatography (MRCP), bile ducts, pancreas
http://ismj.bpums.ac.ir/article-1-184-en.html
http://ismj.bpums.ac.ir/article-1-184-en.pdf
Bushehr University of Medical Sciences
Iranian South Medical Journal
1735-4374
1735-6954
12
2
2009
12
1
Applications of Depleted Uranium in the first and second Persian Gulf Wars: a review article
157
167
FA
Abdolhamid
Behrouzi
N
Firouzeh
Farashbandi
Firoozehz@yahoo.com
Y
Background: Depleted uranium (DU) is a byproduct of natural uranium enrichment process. Its unique characteristics (e.g. high density) caused to use in civilian and military fields extensively. In the military fields, DU is used in the bullets and projectiles war hats. The munitions containing DU were used in the recent wars, more strikingly in the Middle East region (first and second Persian Gulf wars, and Afghanistan). Due to its biological impacts, this study aimed to assess biological effects of DU using scientometrics by investigating papers indexed in Pubmed from 1990-2008, to reveal the number and type of articles and also the important dimensions of DU biological impacts as well as the core issues. Methods: In this descriptive epidemiologic study, quantitative methods (counting frequency of words) and scientometrics were used. Sample size was the total of the articles indexed in Pubmed during 1991- 2008, containing the terms "Gulf War" and "Depleted Uranium" in their title or keywords. Results: The most compromised body systems were urinary, nervous and cardiovascular. Other systems such as endocrine, musculoskeletal, immune and respiratory were also mentioned. Conclusion: Highly controversial results which have been stated in the surveyed articles about DU biological and environmental impacts caused the authors to recommend long term investigations for assessing its effects on recurrence to reveal potential late effects of DU.
first Persian Gulf War, second Persian Gulf War, Depleted Uranium (DU), biological effects
http://ismj.bpums.ac.ir/article-1-185-en.html
http://ismj.bpums.ac.ir/article-1-185-en.pdf
Bushehr University of Medical Sciences
Iranian South Medical Journal
1735-4374
1735-6954
12
2
2009
12
1
A case with crossed fused ectopic kidney
168
171
FA
Sekineh
Asadi
sakineh_ak16@yahoo.com
Y
Jamshid
Saberi
N
Khadijeh
Ghasemi
N
Kolsoum
Afrasiabi
N
Reza
Nemati
N
Crossed fused ectopic kidneys are a rare renal anomaly. The autopsy prevalence of renal ectopia is more than clinical prevalence. Most presenting symptoms of crossed ectopic kidneys are nonspecific and most cases remain asymptomatic through their life and are diagnosed incidentally. The presented case was a 5 years old girl who suffered from episodic left flank pain that imaging findings on sonography and also technetium-99m dimercaptosuccinic acid (DMSA) scintigraphy indicated crossed fused ectopic kidneys.
crossed ectopic kidney, sonography, technetium-99m dimercaptosuccinic acid (DMSA) scan, congenital anomaly
http://ismj.bpums.ac.ir/article-1-186-en.html
http://ismj.bpums.ac.ir/article-1-186-en.pdf