Bushehr University of Medical Sciences
Iranian South Medical Journal
1735-4374
1735-6954
17
6
2015
1
1
Converging technologies: shaping the future of medicine
1045
1067
FA
Iraj
nabipour
The Persian Gulf Marine Biotechnology Research Center, The Persian Gulf Biomedical Research Center, Bushehr University of Medical Sceince, Bushehr, IRAN<br> Future Studies Group, The Academy of Medical Sciences of the I.R.IRAN
inabipour@gmail.com
Y
Majid
Assadi
The Persian Gulf Nuclear Medicine Research Center, The Persian Gulf Biomedical Research Center, Bushehr University of Medical Sceince, Bushehr, IRAN
N
The miniaturization and virtualization processes drive converging technologies from interactions between the NBIC (Nano, Bio, Info, and Cogno) technologies. The converging technologies stimulate innovation, promote research and development in different fields and produce revolutionary progresses in medicine. These technologies enable us to create contacts between brains and machines, the growth in molecular nanotechnology, the construction of respirocytes, chromallocytes, clottocytes, nanorobotic phagocytes, and nanobots. Nanobots would enter the nucleus of a cell and extract all of the genetic material and replace it with a synthetically produced copy of the original that has been manufactured in a laboratory to contain only non-defective base-pairs. It is predicted that “the regenerative medicine”, as a megatrend, will have an enormous effect on medical technologies and clinical sciences. Regenerative medicine is an application field of converging technologies in translational medicine. It attempts to translate the results of tissue engineering to construct 3D tissues and organs. Regenerative medicine is also an exciting field for induced pluripotent stem cell (iPSC) and promises to bring about a paradigm shift to health care. Accumulating evidence indicates that converging technologies will offer great potentials for regenerative medicine to create innovative treatments for diseases that the traditional therapies have not been effective yet.
Converging Technologies, Biotechnology, Regenerative Medicine, induced Pluripotent Stem Cell Technology (iPSC)
http://ismj.bpums.ac.ir/article-1-623-en.html
http://ismj.bpums.ac.ir/article-1-623-en.pdf
Bushehr University of Medical Sciences
Iranian South Medical Journal
1735-4374
1735-6954
17
6
2015
1
1
Role of the future creative universities in the triple helix of science and technology corridors
1068
1089
FA
Iraj
nabipour
The Persian Gulf Marine Biotechnology Research Center, The Persian Gulf Biomedical Research Center, Bushehr University of Medical Sceince, Bushehr, IRAN<br> Future Studies Group, The Academy of Medical Sciences of the I.R.IRAN
inabipour@gmail.com
Y
Abdolmajid
Mosleh
Department of Business Management, Faculty of Humanisties and Literature, Persian Gulf University, Bushehr, IRAN
N
Majid
Assadi
The Persian Gulf Nuclear Medicine Research Center, The Persian Gulf Biomedical Research Center, Bushehr University of Medical Sceince, Bushehr, IRAN
N
The science and technology corridor is a complex cluster containing universities, science parks, research centers, high-tech companies, venture capital, institutional and physical infrastructures, and human capital in a defined geography with its unique management and legal structure in association with the business space and knowledge-based products. In fact, the science and technology corridor reflects the concept of development based on the knowledge region (the especial region for science and technology). The knowledge region is clearly a triple helix phenomenon par excellence: universities, governments and businesses combine their efforts to construct a common advantage which they would not be able to offer on their own. The future creative universities in connection with the knowledge city-regions not only will deal with innovation and entrepreneurial training but also produce a competitive, vibrant environment with high indices for quality of life and full of green technologies. In this article, we will present functional interactions of the creative universities in the triple helix, particularly the missions for the Iranian universities of medical sciences. As a theoretical model, the complex interactions of Bushehr University of Medical Sciences and Health Services with Bushehr Science and Technology Corridor will be discussed.
Science, Technology, Corridor, Knowledge
http://ismj.bpums.ac.ir/article-1-624-en.html
http://ismj.bpums.ac.ir/article-1-624-en.pdf
Bushehr University of Medical Sciences
Iranian South Medical Journal
1735-4374
1735-6954
17
6
2015
1
1
Correlation between serum levels of uric acid with bone turnover markers in postmenopausal women
1090
1099
FA
Fariba
Karimi
The Endocrinology and Metabolism Research Center, Shiraz University of Medical Sciences, Shiraz, IRAN
N
Firooze
Mozhde
The Persian Gulf Tropical Medicine Research Center, Bushehr university of Medical Sciences, Bushehr, IRAN
N
Hussain
Darabi
The Persian Gulf Tropical Medicine Research Center, Bushehr university of Medical Sciences, Bushehr, IRAN
N
Mohammadreza
Kalantar hormozi
The Persian Gulf Tropical Medicine Research Center, Bushehr university of Medical Sciences, Bushehr, IRAN
N
Samad
Akbarzadeh
The Persian Gulf Tropical Medicine Research Center, Bushehr university of Medical Sciences, Bushehr, IRAN
N
Afshin
Ostovar
The Persian Gulf Tropical Medicine Research Center, Bushehr University of Medical Sciences, Bushehr, IRAN
N
Majid
Assadi
The Persian Gulf Nuclear Medicine Research Center, Bushehr University of Medical Sciences, Bushehr , IRAN
N
Katayon
Vahdat
The Persian Gulf Tropical Medicine Research Center, Bushehr university of Medical Sciences, Bushehr, IRAN
N
Iraj
nabipour
The Persian Gulf Marine Biotechnology Research Center, Bushehr university of Medical Sciences, Bushehr, IRAN
inabipour@gmail.com
Y
Background: There is accumulation evidence regarding the protective role of higher serum uric acid in bone loss and osteoprotic fractures. This effect may arise from its antioxidant properties. The main aim of this study was to investigate serum uric acid in relation to bone turnover markers in postmenopausal women.
Material and methods: A total of 382 postmenopausal women who participated in a prospective study were selected randomly from Bushehr port. Serum uric acid levels and biochemical markers including serum alkaline phosphatase were measured. Circulating levels of CrossLaps, osteocalcin and hsCRP were also measured by ELISA.
Results: Uric acid levels had significant correlation with osteocalcin & alkaline phosphatase. However, no correlation could be found between uric acid and CrossLaps. The correlation between uric acid & osteocalcin (r= 0.25, P=0.0001) & alkaline phosphatase(r=0.12, P=0.019) remained after adjustment with age, BMI & hsCRP.
Conclusion: Serum uric acid had a positive and significant correlation with osteoblastic born turnover markers. Hence, the role of uric acid in osteoblastic function should be investigated.
uric acid, osteocalcin, CrossLaps, alkaline phosphatase, menopause
http://ismj.bpums.ac.ir/article-1-625-en.html
http://ismj.bpums.ac.ir/article-1-625-en.pdf
Bushehr University of Medical Sciences
Iranian South Medical Journal
1735-4374
1735-6954
17
6
2015
1
1
The Predictive role of omentin-1, visfatin and adiponectin in osteoporosis-associated bone loss in postmenopausal women: a prospective cohort study
1100
1112
FA
Fariba
Karimi
The Endocrinology and Metabolism Research Center, Shiraz University of Medical Sciences, Shiraz, IRAN
N
Elnaz
Aminfar
The Persian Gulf Tropical Medicine Research Center, Bushehr university of Medical Sciences, Bushehr, IRAN
N
Hussain
Darabi
The Persian Gulf Tropical Medicine Research Center, Bushehr university of Medical Sciences, Bushehr, IRAN
N
Mohammadreza
Kalantar hormozi
The Persian Gulf Tropical Medicine Research Center, Bushehr university of Medical Sciences, Bushehr, IRAN
N
Samad
Akbarzadeh
The Persian Gulf Tropical Medicine Research Center, Bushehr university of Medical Sciences, Bushehr, IRAN
N
Katayon
Vahdat
The Persian Gulf Tropical Medicine Research Center, Bushehr university of Medical Sciences, Bushehr, IRAN
N
Afshin
Ostovar
The Persian Gulf Tropical Medicine Research Center, Bushehr university of Medical Sciences, Bushehr, IRAN
N
Majid
Assadi
The Persian Gulf Nuclear Medicine Research Center, Bushehr University of Medical Sciences, Bushehr , IRAN
N
Iraj
nabipour
The Persian Gulf Marine Biotechnology Research Center, Bushehr university of Medical Sciences, Bushehr, IRAN
inabipour@gmail.com
Y
Background: There is conflicting evidence regarding adipocytokines in relation to bone mineral density (BMD) and osteoporotic fractures in women and men. Recently, we found that circulating omentin-1 levels had an inverse correlation with BMD at the lumbar spine in postmenopausal women. The main aim of this study was to investigate the predictive role of adipocytokines for bone loss and incident osteoporosis among postmenopausal women after a 5.8- year follow-up.
Material and methods: A total of 382 healthy postmenopausal women who participated in a prospective cohort study were followed for 5.8 years. Omentin-1, visfatin, adiponectin, and the receptor activator of nuclear factor-κB ligand (RANKL) were measured by highly specific enzyme-linked immunosorbent assay methods. BMD was determined for the lumbar spine (L2–L4) and the proximal femur using dual-energy X-ray absorptiometry.
Results: The femoral neck and lumbar spine bone loss at the 5.8-year follow-up were 1.0% and 3.4% per year.In logistic regression analysis, none of the studied adipocytokines predicted BMD at any skeletal sites or incident osteoporosis at the femoral neck or lumbar area after 5.8 year follow-up. However, RANKL levels predicted BMD at the lumbar spine (β=−0.19, p=0.003) and femoral neck (β=-0.341, p=0.018) after controlling for body mass index.
Conclusion: Serum levels of omentin-1, visfatin and adiponectin did not predict bone loss and incident osteoporosis among postmenopausal women.
omentin-1, visfatin, adiponectin, menopause, bone density
http://ismj.bpums.ac.ir/article-1-626-en.html
http://ismj.bpums.ac.ir/article-1-626-en.pdf
Bushehr University of Medical Sciences
Iranian South Medical Journal
1735-4374
1735-6954
17
6
2015
1
1
Optimization of the geometry and composition of a neutron system for treatment by Boron Neutron Capture Therapy
1113
1119
FA
Rohollah
Gheisari
Physics Department, Faculty of Science, Persian Gulf University, Bushehr, IRAN
gheisari@pgu.ac.ir
Y
Mohmmadmehdi
Firoozabadi
Department of Physics, Faculty of Science, Birjand University, Birjand, IRAN
N
Habib
Mohammadi
Department of Physics, Faculty of Science, Birjand University, Birjand, IRAN
N
Background: In the field of the treatment by Boron Neutron Capture Therapy (BNCT), an optimized neutron system was proposed. This study (simulation) was conducted to optimize the geometry and composition of neutron system and increase the epithermal neutron flux for the treatment of deep tumors is performed.
Materials and Methods: A neutron system for BNCT was proposed. The system included 252Cf neutron source, neutron moderator/reflector arrangement, filter and concrete. To capture fast neutrons, different neutron filters Fe, Pb, Ni and PbF2 with various thicknesses were simulated and studied. Li (with 1 mm thick) was used for filtering of thermal neutrons. Bi with thickness of 1 cm was used to minimize the intensity of gamma rays. Monte Carlo simulation code MCNPX 2.4.0 was used for design of the neutron system and calculation of the neutron components at the output port of the system.
Results: For different thicknesses of the filters, the fast neutron flux, the epithermal and thermal flux were calculated at the output port of the system. The spatial distribution of the fast neutron flux, the epithermal flux and gamma flux in human head phantom with the presence of 40 ppm of 10B were obtained. The present calculations showed that Pb filter (about 1 cm) at the output port is suitable for fast neutron capture. The thickness of Li filter was determined due to its high absorption cross-section in thermal region. Bi was used as a gamma filter by the reason of it is good for shielding gamma rays, while having high transmission epithermal neutrons.
Conclusion: The epithermal neutron flux has enhanced about 38 percent at the output port of the present system, compared with recent system proposed by Ghassoun et al. At 2 cm depth inside the head phantom, the neutron flux reaches a maximum value about . At this depth, the ratio of the thermal neutron flux to the epithermal flux is about three times, that suggests such a neutron system to treat tumors in the proximity of the depth. In the presence of 10B in the brain, at 2 cm depth the neutron absorption takes place more than other areas of the brain, and consequently the thermal flux is depressed uniformly in the head phantom. Due to high LET and RBE of alpha and 7Li particles (obtained by reaction of boron-neutron), the tumor at the mentioned depth is damaged rather than the around.
252Cf source, neutron and photon filters, neutron system, epithermal neutron, head phantom, Monte Carlo simulation
http://ismj.bpums.ac.ir/article-1-627-en.html
http://ismj.bpums.ac.ir/article-1-627-en.pdf
Bushehr University of Medical Sciences
Iranian South Medical Journal
1735-4374
1735-6954
17
6
2015
1
1
Study of the effect of GABAA receptore and glial inhibition on behavioral responses in CCI model of neuropathic pain in rat
1120
1134
FA
Mehi
Sadeghi
Department of physiology, School of Medicine, Bushehr University of Medical Sciences, Bushehr, IRAN<br> Department of Neurophysiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, IRAN
N
Homa
Manaheji
Neuroscice Research Center, Shahid Beheshti University of Medical Sciences, Tehran, IRAN<br> Department of Neurophysiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, IRAN
hshardimanaheji@yahoo.com
Y
Abbad
Haghparast
Neuroscice Research Center, Shahid Beheshti University of Medical Sciences, Tehran, IRAN
N
Jalal
Zaringhalam
Neuroscice Research Center, Shahid Beheshti University of Medical Sciences, Tehran, IRAN<br> Department of Neurophysiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, IRAN
N
Samad
Nazemi
Department of physiology, School of Medicine, Sabzevar University of Medical Sciences, Sabzevar, IRAN
N
Zahra
Bahari
Department of Neurophysiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, IRAN
N
Background: The mechanisms underlying neuropathic pain are complex and remain controversial. From the proposed mechanisms we can refer to loss of GABAergic inhibition and glial activation in the spinal dorsal horn. As for the discrepancies in the neuropathic pain mechanisms, in the present study, we examined whether the GABA-A receptor agonist muscimol and glial inhibitor pentoxifylline would modify behavioral tests in rats with Chronic Constriction Injury (CCI).
Material and Methods: In this study male wistar rats (200-250 g) were used and for neuropathy induction, the CCI model (Bennett method, 1988) was used. In the sham group, after exposing the sciatic nerve, surgery region was closed. Pentoxifylline was administered one day before neuropathy to 14 days after CCI (30 mg/kg daily) and behavioral tests (plantar test and von frey) were performed one day before surgery and then on days 1, 4, 7 and 14 after surgery and 30 minutes after pentoxifylline adminstration .In groups that received muscimol with doses 0.5, 1 and 2 mg/kg on day 14 after CCI, behavioral tests were experienced before and 30 minutes after drug administration.
Results: Behavioral assessment indicated that CCI induce symptoms of neuropathic pain but both muscimol and pentoxifylline could reduce pain behavioral responses. It seems that this reduction of muscimol (1 and 2 mg/kg) was more effective in thermal hyperalgesia than pentoxifylline, and for pentoxifylline (30 mg/kg) was more effective in mechanical allodynia than muscimol. Conclusion: present data showed that muscimol via activation of GABA-A receptors and pentoxifylline via glial inhibition reduced behavioral symptoms of neuropathic pain after spinal cord injury.
neuropathic pain, hyperalgesia, allodynia, CCI, muscimol, pentoxifylline
http://ismj.bpums.ac.ir/article-1-628-en.html
http://ismj.bpums.ac.ir/article-1-628-en.pdf
Bushehr University of Medical Sciences
Iranian South Medical Journal
1735-4374
1735-6954
17
6
2015
1
1
Study of mesanchymal stem cells derived from human umbilical cord vein wall and determining the Process of differentiation to cartilage and bone
1135
1142
FA
MohammadAli
Zare
Department of biology and Anatomical Sciences, School of Medicin, Bushehr University of Medical Sciences, Bushehr, IRAN
N
Mohammad reza
Baghaban Eslaminejad
Cellular Research Center,Department of Stem Cell, Tehran, IRAN<br> Department of Stem Cell and Developmental Biology, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, IRAN
eslami@royaninstitute.org
Y
Ahmad
Hosseini
Cellular and molecular Biology Research Center, Shahid Beheshti University of Medical Sciences, Teheran, IRAN
N
Background: Mesenchymal stem cells (MSCs) comprise a rare population of multipotent progenitors capable of supporting hematopoiesis and differentiating into three (osteogenic, adipogenic, and chondrogenic) or more (myogenic, cardiomyogenic, etc.) lineages. Due to this ability, MSCs appear to be an attractive tool in the context of tissue engineering and cell-based therapy. Currently, bone marrow represents the main source of MSCs for both experimental and clinical studies. The purpose of this study was isolation and quantitative comparison of mesenchymal stem cells derived from umbilical vein.
Materials and Methods: In this study, 35 samples of umbilical cord of healthy full- term newborn were studied.
Results: The cells had fibroblastoid like appearance and had revealed the potential to differentiate into three linage of bone, Adipose and cartilage. Surface markers for mesenchymal nature were their demonstratives.
Conclusion: Based on our findings the mesenchymal stem cells, from umbilical vein wall can be isolated, cultured and differentiated into three categories of bone, cartilage and adipose.
Mesenchymal Stem Cells, Human Umbilical Cord, Umbilical Vein, differentiation
http://ismj.bpums.ac.ir/article-1-629-en.html
http://ismj.bpums.ac.ir/article-1-629-en.pdf
Bushehr University of Medical Sciences
Iranian South Medical Journal
1735-4374
1735-6954
17
6
2015
1
1
Factors affecting inter-birth in rural areas of Zarrindasht (Fars province)
1143
1152
FA
Najaf
Zare
Department of Biostatistics, Infertility Research Center, Shiraz University of Medical Sciences, Shiraz, IRAN
najafzare@sums.ac.ir
Y
Mojtaba
Soltani
Department of Biostatistics, Medical School, Shiraz University of Medical Sciences, Shiraz, IRAN
N
Mehrab
Sayadi
Department of Biostatistics, Medical School, Shiraz University of Medical Sciences, Shiraz, IRAN
N
Abdolreza
Rajaeefard
Department of Epidemiology, School of Health, Shiraz University of Medical Sciences, Shiraz, IRAN
N
Background: Proper time intervals among pregnancies has a major role in family provision. Investigating the dynamism of the birth spacing, identify factors and their effects on the time interval among pregnancies and determination of optimal spacing is the utmost importance for health planners and families. The purpose was to study the abortion and stillbirth due to short inter-birth spacing, factors affecting inter-births and optimal birth spacing.
Material and Methods: In 2005, a cross-sectional study was conducted in a population of 1064 women of childbearing age in Fars province by multistage random sampling. Information on reproductive history was collected by resident health workers. Variables affecting inter-births were checked by the frailty recurrent events model using S-plus 2000 and SPSS 13 software.
Results: The rate of abortion or stillbirth was 1.7%, which fell with increasing interval between births and reached their lowest (1.9%) at 36-48 months. Mother's level of education, duration of breastfeeding of previous infant, maternal age at birth and father's job showed positive correlation with inter-birth. Mother's age at marriage, having a job other than household, socioeconomic status was negatively correlated with the the time interval to the next delivery. With increasing birth order, inter-birth of each delivery with regard to previous delivery increased on average by one to two months.
Conclusion: The risk of abortion and stillbirth were observed more in subsequent delivery with very low or very long gap interval.
reproductive history, optimal spacing, Cox model, frailty model, inter-birth
http://ismj.bpums.ac.ir/article-1-630-en.html
http://ismj.bpums.ac.ir/article-1-630-en.pdf
Bushehr University of Medical Sciences
Iranian South Medical Journal
1735-4374
1735-6954
17
6
2015
1
1
Study of ethA gene sequence in Ethionamide resistant clinical isolates of Mycobacterium tuberculosis
1153
1159
FA
Somaye
Moazemi
Microbiology Department, Faculty of Science, Islamic Azad University of Arak, Arak, IRAN
N
Mohammad
Arjomandzadegan
Department of microbiology, Tuberculosis and Pediatric Infectious Disease Research Center , Arak University of Medical Sciences,Arak, IRAN
arjomandzadegan@arakmu.ac.ir
Y
Azam
Ahmadi
Tuberculosis and Pediatric Infectious Disease Research Center, Arak, Department of Molecular Genetics, Faculty of Biological Science, Tarbiat Modares University, Tehran, IRAN
N
Maryam
Tayebun
Microbiology Department, Faculty of Science, Islamic Azad University of Arak, Arak, IRAN
N
Mana
Shojapor
Research Center of Molecular Medicine, University of Medical Sciences, Arak, IRAN
N
Background: Ethionamide (ETH) is a structural analog of isoniazid and secondary line anti-tuberculosis drugs. Both of Ethionamide and isoniazid target INHA protein in mycobacterium tuberculosis. This protein involved in mycolic acid biosynthesis. The purpose of this study is evaluation of sequence of ethA gene in order to detect mutations related to resistance to isoniazide in clinical mycobacterium tuberculosis isolates.
Material and Methods: In this study were evaluated 27 resistance and sensitive isolates to ethionamide. Because of length of this fragment (1470bp) were performed three reactions for each sample with ethA-10, ethA-8, ethA-9, ethA-4, ethA-5 and ethA-1 specific primers in PCR reaction.
Results: From 27 used clinical isolates, 23 strains were resistant and 4 isolates were susceptible to ETH. Results of electrophoresis were proved proper selection of primers and PCR conditions. DNA sequencing results were determined mutations at some points of the gene in the resistant isolates to ETH, but none of susceptible strains harbored mutations.
Conclusion: According to the results it was proved that any mutations in each point of ethA gene could cause resistance to Ethionamid. Rapid molecular detection of the resistance is possible only via complete sequencing of total length of this gene.
Mycobacterium tuberculosis, Drug resistant, Ethionamide, sequencing
http://ismj.bpums.ac.ir/article-1-631-en.html
http://ismj.bpums.ac.ir/article-1-631-en.pdf
Bushehr University of Medical Sciences
Iranian South Medical Journal
1735-4374
1735-6954
17
6
2015
1
1
Histopathological effects of sodium nitrite on the spleen of male and female rats
1160
1167
FA
Fateme
Juibar
Young Researchers and Elite Club, Kazerun Branch, Islamic Azad University, Kazerun, IRAN
Dr.f.juibar@gmail.com
Y
Ali
Tavakoli kazerooni
Department of biology & Laboratory Science, Kazerun Branch, Islamic Azad University, Kazerun, IRAN
N
Ali
Ghorbani ranjbary
Young Researchers and Elite Club, Kazerun Branch, Islamic Azad University, Kazerun, IRAN
N
Background: Nitrite and nitrate are common additives in meat processed products. In spite of all technological advantages of nitrite, creation of nitrosamine carcinogenic substances causes a lot of concerns for use of these additives. In this study, the histopatological effects of sodium nitrite on the splenic tissue in adult male and female rats were evaluated.
Material and method: In recent studies, 60 adult male and female rats strain Vistar, divided in 6 groups of 10. They were examined for 60 day, and they (male and female rats separately) were divided in 175 mg/kg/day dose recipient group, 350 mg/kg/day dose recipient group and control group which was absorbed nitrite through drinking water. At the end of day 60, using cotton dipped in ether in the jar of anesthesia, were anesthetized. After anesthesia, blood from the left ventricle was taken .the spleen was taking out of body, and then tissue sections were prepared for testing tissue changes. The samples were stained with Hematoxilin- Eozin method. In both sex, factors like morphometric and morphologic from spleen tissue, body weight changes before and after test and blood NOx level was checked. After data collection, all data was analyzed by SPSS statistical software version 17 with using Independent sample t-test and ANOVA. P value of less than 0.05 were reported as statistically significant.
Result: The results showed that consume of sodium nitrite, cause background inflammation type of Mononuclear in both sex, especially around the pulp. Also, in both sex NOx levels in the blood of animals in the group receiving 350 mg/ kg and group receiving 175 mg of sodium nitrite per kg compared with the control group, significantly increased (p ≤ 0.05).
Conclusion: Considering of this study and other studies, Nitrite can cause damage to blood vessels, liver, spleen and other organs. Also sodium nitrite has to switching to other food preservatives.
Inflammation, Nitric oxide, Sodium nitrite, Rat, Spleen
http://ismj.bpums.ac.ir/article-1-632-en.html
http://ismj.bpums.ac.ir/article-1-632-en.pdf
Bushehr University of Medical Sciences
Iranian South Medical Journal
1735-4374
1735-6954
17
6
2015
1
1
Cloning and expression of aequorin photoprotein using intein tag
1168
1175
FA
Elah sadat
Seyed Hosseini
Department of Bioscience and Biotechnology, Mallek Ashtar University of Technology, Tehran, IRAN
N
Mehdi
Zeinoddini
Department of Bioscience and Biotechnology, Mallek Ashtar University of Technology, Tehran, IRAN
zeinoddini52@mut.ac.ir
Y
Zeinoddini
Fallah
Department of Bioscience and Biotechnology, Mallek Ashtar University of Technology, Tehran, IRAN
N
Alireza
Saeedinia
Department of Bioscience and Biotechnology, Mallek Ashtar University of Technology, Tehran, IRAN
N
Background: Intein (INT), is the internal parts of the protein which can be separated from the immature protein during protein splicing process. This sequence requires no specific enzyme or cofactor for separation. This protein sequence and their characteristic of self-cleavage by thiol induction, temperature and pH changes is used for protein purification. The advantage of this method compared to the other protein purification methods is that it doesn’t require any protease enzyme and protease removal steps that make this method important economically. In this study, aequorin photoprotein was hybridized with INT in molecular form and its expression was evaluated.
Materials and Methods: In this study, aequorin coding gene that was cloned in pET21-a in the previous studies, was cloned in pTYB21 vector containing INT tag by specific primers and restriction enzymes. Then the resulting pTY-aequarin was transformed to the ER2566 expression strain and cloning accuracy was confirmed by electrophoresis, western blotting and sequencing.
Results: The photoprotein aequorin was cloned into SapI/PstI restriction site of pTYB21 plasmid accurately and successfully. Aequorin- INT hybrid protein expression confirmed using traditional methods.
Conclusion: The photoprotein aequorin constract in fused with INT confirmed by molecular methods. Also rate of Aequorin- INT expression determined about %25 of cell total protein.
Intein, Protein splicing, Cloning, Expression, Aequorin photoprotein
http://ismj.bpums.ac.ir/article-1-633-en.html
http://ismj.bpums.ac.ir/article-1-633-en.pdf
Bushehr University of Medical Sciences
Iranian South Medical Journal
1735-4374
1735-6954
17
6
2015
1
1
Relation cellular- molecular between serum IL10 levels and hyperalgesia variation in adjuvant- induced arthritis
1176
1187
FA
Zenab
Akhtari
Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, IRAN
N
Jalal
Zaringhalam
Physiology Department, Neuroscience Research Center, Shahid Beheshti University of Medical Science, Tehran, IRAN
jzaringhalam@yahoo.com
Y
Akram
Eidi
Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, IRAN
N
seyd Ali
HaeriRuhani
Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, IRAN
N
Homa
Manaheji
Physiology Department, Neuroscience Research Center, Shahid Beheshti University of Medical Science, Tehran, IRAN
N
Elahe
Tekieh
Physiology Department, Neuroscience Research Center, Shahid Beheshti University of Medical Science, Tehran, IRAN
N
Background: Regarding to the important anti-inflammatory role of IL10 during inflammation process and hyperalgesia and edema variation during CFA-induced arthritis and also the increase of Spinal mu opioid receptor (mOR) expression, in this study researchers investigate the role of serum IL10 level on mOR expression and edema and hyperalgesia variation during different stages of Complete Freund`s Adjuvant (CFA) - induced arthritis in male Wistar rats.
Materials and Methods: Mono-arthritis was induced by CFA and inflammatory symptoms (hyperalgesia and edema) were assessed on 0, 3, 7, 14th and 21st days of study. Anti-IL10 was administered during the 21 days of study in different experimental groups. mOR expression were detected by western blotting on 0, 3,7, 14th and 21st days of study. Data was analyzed by SPSS statistical software version 19 with using one way ANOVA (post hoc Tokey's).
Results: Our results showed that anti-IL10 administration in AA group (Adjuvant Arthritis) caused an increase in the paw volume and hyperalgesia until 21st of study. Our study stated that there were no significant differences in spinal mOR expression between AA and AA+anti-IL10rats.
Conclusion: Our study confirmed that anti-IL10administration caused to hyperalgesia and edema during AA inflammation. Also these findings suggested that mOR expression increased in chronic phase of AA inflammation, however an increase in the level of spinal mu opioid receptor (mOR) expression during AA inflammation is not mediated directly via the effect of serum IL-10.
Inflammation, Hyperalgesia, Edema, CFA, IL-10, mOR expression
http://ismj.bpums.ac.ir/article-1-634-en.html
http://ismj.bpums.ac.ir/article-1-634-en.pdf
Bushehr University of Medical Sciences
Iranian South Medical Journal
1735-4374
1735-6954
17
6
2015
1
1
Correlation between metabolic syndrome and serum concentration of vitamin 25(OH)D
1188
1194
FA
Afsaneh
Talaei
Department of Endocrinology, Thyroid disorders research center, Arak university of medical scienceArak, IRAN
talaei@arakmu.ac.ir
Y
Navidreza
Mashayekhi
Department of Cardiology, School of medicine, Arak university of medical science, Arak, IRAN
N
Background: The simultaneous occurrence of risk factors for both type II diabetes and coronary artery disease show the existence of "metabolic syndrome".The studies have been shown that vitamin D deficiency has an inverse correlation with the components of metabolic syndrome such as BMI,systolic blood pressure,waist,fatsing blood glucose,LDL cholesterol and insulin resistance. So, this sudy evaluated the association between metabolic syndrome and vitamin D deficiency.
Materials and Methods: In this cross – sectional study among overweight patients referred to private and public clinics,70 patients with metabolic syndrome based on ATPIII criteria and 70 overweight healthy participants were selected and mean of 25 hydroxyvitamin D level in the two groups were compared. Laboratory tests for lipid profile and fasting blood glucose and vitamin D level and also examinations for waist, weight,height were done. Mann-Whitney-U and Student-t-test were used to evaluate the relationship of vitamin D level and metabolic syndrome.
Results: Participants in this study were 63% female.The prevalence of vitamin D deficiency was 71.4% and 26.2% in metabolic and control groups, respectively (P 0.001). Mean of vitamin D was 20±22 and 27±17 ng / ml in metabolic and control groups respectively (P 0.01).
Conclusion: The findings showed a significant correlation between vitamin D deficiency and metabolic syndrome and vitamin D deficiency has a role in establishment of metabolic syndrome through different mechanisms.
Diabetes, metabolic syndrome, deficiency,vitamin D
http://ismj.bpums.ac.ir/article-1-635-en.html
http://ismj.bpums.ac.ir/article-1-635-en.pdf
Bushehr University of Medical Sciences
Iranian South Medical Journal
1735-4374
1735-6954
17
6
2015
1
1
Relation between bone mineral density and coronary artery disease
1195
1202
FA
Masoome
Tohodi
Endocrinology and Metabolism Research Center, Namazi Hospital , Shiraz university of medical science, Shiraz, IRAN
tohidim@sums.ac.ir
Y
Mohammad Housein
Dabbaghmanesh
Endocrinology and Metabolism Research Center, Namazi Hospital , Shiraz university of medical science, Shiraz, IRAN
N
Javad
Kojuri
Depatment of Cardiology, Namazi Hospital, Shiraz University of Medical Science, Shiraz, IRAN
N
Masoome
Khedri
Depatment of Internal Medicine, Shool of Medicine, AhvazUniversity of Medical Science, Ahvaz, IRAN
N
Background: Osteoporosis and cardiovascular disease are the two important life threatening factors that cause morbidity and mortality. Previous studies had showed that bone mineral density is decreased with progression of atherosclerosis, and cardiovascular disease is more common in the osteoporotic patients. The aim of this study is to evaluate the relationship between bone mineral density and coronary artery stenosis in patients who underwent coronary angiography .
Materials and Methods: In this study 197 patients (111female and 86 male) that underwent coronary angiography and bone mineral densitometry were evaluated. According to angiographic data ,patients were divided to two group :first group had coronary stenosis in ≥50% in one or more major coronary arteries and second group with normal coronary artery or stenosis <50%. Mean bone mineral density(gr/cm2)was measured in the lumbar spine and hip by using Dual Energy X-ray Absorptiometry (DEXA) and T-score was determined .T-score > -1 as normal , T-score -1 to -2.5 as osteopenia, and T-score <-2.5 as osteoporosis were described. Finally the relationship between coronary artery stenosis and bone mineral density was evaluated by SPSS statistical software version 15 with using suitable statistical test.
Results: The mean age of sample was 57±11.32). 33% of patients had osteoporosis in lumbar spine and 24.2% of them in femoral neck.Significant coronary artery stenosis were seen in 67.9% of patients. There were no significant difference between lumbar and hip bone mineral density in patients with significant coronary disease and subjects without coronary artery disease.
Conclusion: There were no associations between coronary artery disease and osteoporosis. Further studies are recommended for confirmation of this relationship.
Coronary artery disease, Bone mineral density, Osteoporosis, Osteopenia
http://ismj.bpums.ac.ir/article-1-636-en.html
http://ismj.bpums.ac.ir/article-1-636-en.pdf
Bushehr University of Medical Sciences
Iranian South Medical Journal
1735-4374
1735-6954
17
6
2015
1
1
Electrochemical removal of hexavalent chromium from wastewater using Platinum-Iron/Iron-carbon nanotubes and bipolar Electrodes
1203
1212
FA
Hoshyar
Hossini
Depatment of Environmental Health Engineering, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, IRAN
N
Abbas
Rezaee
Depatment of Environmental Health Engineering, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, IRAN.
rezaee@modares.ac.ir
Y
seyd Enayat
Hashemi
Environmental Health Engineering Group, Faculty of Medical Sciences, Bushehr University of Medical Science, Bushehr, IRAN
N
Background: In recent decades, electrocoagulation (EC) has engrossed much attention as an environmental-friendly and effectiveness process. In addition, the EC process is a potential suitable way for treatment of wastewater with concern to costs and environment. The object of this study was electrochemical evaluation of chromium removal from industrial wastewater using Platinum and carbon nanotubes electrodes.
Materials and Methods: The effect of key variables including pH (3–9), hexavalent chromium concentration (50–300 mg/l), supporting electrolyte (NaCl, KCl, Na2CO3 and KNO3) and its dosage, Oxidation-Reduction variations, sludge generation rate and current density (2–20 mA/cm2) was determined. Results: Based on experimental data, optimum conditions were determined in 20, 120 min, pH 3, NaCl 0.5% and 100 mg/L initial concentration of chromium.
Conclusions: Removal of hexavalent chromium from the wastewater could be successfully performanced using Platinum-Iron/Iron-carbon nanotubes and bipolar Electrodes.
Electrochemical removal, hexavalent chromium, platinum electrode, carbon nanotubes electrode.
http://ismj.bpums.ac.ir/article-1-637-en.html
http://ismj.bpums.ac.ir/article-1-637-en.pdf
Bushehr University of Medical Sciences
Iranian South Medical Journal
1735-4374
1735-6954
17
6
2015
1
1
Relation between endometrial thickness with serum estradiol and progesterone levels in patients visited at Ob;Gyn clinic of Shahid Sadoughi hospital
1213
1222
FA
Robab
Davar
Obstetric & Gynecology Department, School of Medicine,Yazd university of medical sciences, Yazd, IRAN
N
Razeah
Dehghani Firouzabadi
Obstetric & Gynecology Department, School of Medicine,Yazd university of medical sciences, Yazd, IRAN
N
Kefayat
Chaman-Ara
Obstetric & Gynecology Department, School of Medicine,Yazd university of medical sciences, Yazd, IRAN
chamanarak109859@gmail.com
Y
Background: The endometrial thickness is an important determinant for the successful implantation and pregnancy. This research was aimed to evaluate relationship between the endometrial thickness with serum estradiol and progesterone levels in patients visited in Ob&Gyn clinic of Shahid Sadoughi hospital.
Material and Methods: Enrolled in the study were 440 patients visited in Ob&Gyn clinic of Shahid Sadoughi hospital between Jan. 2011 to Sep. 2012. Only patients with regular menstrual cycle were included in study. Patients with myoma, adenomyosis, endometrial polyps or other uterine anomaly, those who smoked, women with BMI greater than 30 and who were taking medications that could affect endometrial thickness were excluded. Endometrial thickness one day before and 5-7 days after ovulation was measured by using trans-vaginal ultrasonography. Also, serum estradiol and progesterone levels were measured in 5-7 days after ovulation. Data analysis was done through SPSS version 16 with using descriptive statistics and ANOVA.
Results: The mean age of patients was 27.37±4.44. Endometrial thicknesses were 9.80±0.80 and 10.13±1. one day before and 5-7 days after ovulution, respectively. 5% of patients had thin endometrium (<7 mm) in two measurements. Serum estradiol and progesterone levels were respectively 169.88±17.64 pg/ml and 7.32±6.93 mg/ml. There were no significant association between endometrial thickness and age, and also endometrial thickness and progesterone level. The same relation between endometrial thickness and estradiol serum level was observed only in age group of <20 years. (P=0.000)
Conclusion: The results showed that serum stradiol and progesterone levels can not be the determinants of endometrial thickness in women.
Endometrium, Ultrasonography, Estrogen, Progesterone
http://ismj.bpums.ac.ir/article-1-638-en.html
http://ismj.bpums.ac.ir/article-1-638-en.pdf
Bushehr University of Medical Sciences
Iranian South Medical Journal
1735-4374
1735-6954
17
6
2015
1
1
Evaluation of physical, chemical and microbial quality of distribution network drinkingwater in Bushehr, Iran
1223
1235
FA
Elham
Shabankareh fard
Department of Environmental Health Engineering, Faculty of Health, Bushehr University of Medical Sciences, Bushehr, IRAN
N
Roqayeh
Hayati
Department of Environmental Health Engineering, Faculty of Health, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IRAN
N
Sina
Dobaradaran
Systems Environmental Health, Oil, Gas and Energy Research Center, Bushehr University of Medical Sciences, Bushehr, IRAN<br> The Persian Gulf Marine Biotechnology Research Center, Bushehr University of Medical Sciences, Bushehr, IRAN<br> Department of Environmental Health Engineering, Faculty of Health, Bushehr University of Medical Sciences, Bushehr, IRAN
sina_dobaradaran@yahoo.com
Y
Background: The physical, chemical and microbial properties of water are the criteria to consider it as drinking water quality. Unfavorable changes in such parameters may threat consumers' health. The aim of this study is to give a clear view of physical, chemical and microbial quality of distribution network drinking water in Bushehr and compare with national and EPA standards.
Materials and Methods: This descriptive sectional study was done during Sep 2012 to Feb 2013 (6 months). 50 Samples were collected directly from distribution network drinking water in Bushehr. Physical and chemical analyses were done according to standard methods. Multiple tube fermentation method was used to determine fecal and total coliform bacteria and spread plate method was used to measure heterotrophic bacteria.
Results: The mean values of measured parameters were as follow: electrical conductivity 1155.5 µs/cm, turbidity 0.27 NTU, pH 7.12, alkalinity 171.5, total hardness 458.96, calcium hardness 390.96, magnesium hardness 68 mg/L as CaCO3, calcium 156.38, magnesium 16.95, residual chlorine 0.61, chloride 83.26, TDS 577.7, iron 0.115, fluoride 0.48, phosphate 0.059, nitrate 3.08, nitrite 0.003 and sulphate 728.38 mg/L. Total coliform (0), fecal coliform (0) MPN/100 ml and HPC 309.8 CFU/mL. Except TDS and sulphate, all cited results met the national and EPA standards.
Conclusion: Quality of water from distribution network in Bushehr was not problematical from health point of view. However, high TDS and sulphate content may increase diarrhea risk in consumer as well as corrosive effect of water.
Water distribution network, Total coliform, Fical coliform, Sulphate, Bushehr
http://ismj.bpums.ac.ir/article-1-639-en.html
http://ismj.bpums.ac.ir/article-1-639-en.pdf
Bushehr University of Medical Sciences
Iranian South Medical Journal
1735-4374
1735-6954
17
6
2015
1
1
Application of the basic constructs of social cognitive theory for predicting mental health in student of Bushehr University Medical Sciences 2012-13
1236
1251
FA
Makyea
Jamali
Department of Health, Bushehr University of Medical Sciences, Bushehr, IRAN
N
Azita
Noroozi
Department of Health, Bushehr University of Medical Sciences, Bushehr, IRAN
azitanoroozi@yahoo.com
Y
Rahim
Tahmasebi
Department of Health promotion, The Persian Gulf Marine Biotechnology Research Center, Bushehr University of Medical Sciences, Bushehr, IRAN<br> Department of Health, Bushehr University of Medical Sciences, Bushehr, IRAN
N
Background: mental health is one of the health assessment topics in different communities which plays an important role in ensuring the dynamism and efficiency, especially in the students. Thus, the aim of this study is to application of basic constructs of social cognitive theory for predicting mental health in student of Bushehr University Medical Sciences.
Materials and Methods: This cross– sectional study was conducted with using a systematic random sampling method in 428 students of Bushehr University Medical Sciences in 2012-13. Information was collected by using five standard questionnaires including academic self efficacy, academic stress, multidimensional social support, student outcome expectancy and Quality of life (SF-36) scales. After data collection, all data was analyzed by SPSS statistical software with using Pearson correlation coefficient test and multiple linear regressions.
Results: In this study, mental health had a significant correlation with social support (P =0.000, r=0.37), academic stress (P= 0.000, r= -0.45) and academic self-efficacy (P =0.000 , r =0. 24). In the liner regression model, predictor factors of mental health were faculty type and curriculum counseling and noncurriculum counseling evaluation variables and self efficacy (P=0.031, B= 1.49), academic stress (P=0.000, B=- 4.35), and social support constructs (P=0.000, B =4.77). Also, gender, mother's education and father's job had indirect effects to mental health through social support and acceptance quota and curriculum counseling evaluation had indirect effects to mental health through self efficacy.
Conclusion: Utilization of strategies to increase self- efficacy, creating social support environment and also stress reduction particularly with organization of curriculum and non-curriculum counseling sessions can promote mental health in students.
Mental health, self efficacy, Psychological stress, Social support, students
http://ismj.bpums.ac.ir/article-1-640-en.html
http://ismj.bpums.ac.ir/article-1-640-en.pdf
Bushehr University of Medical Sciences
Iranian South Medical Journal
1735-4374
1735-6954
17
6
2015
1
1
Comparing the effectiveness of multimedia & routine educations on exclusive breast feeding in children less than 6 months in health centers of Bushehr city
1252
1260
FA
Sherafat
Akaberian
Midwifery department, School of nursing and midwifery, Bushehr university of medical sciences, Bushehr, IRAN
sh.akabarian@bpums.ac.ir
Y
Tayebeh
Jamand
Nursing department ,School of nursing and midwifery, Boushehr university of medical sciences, Boushehr, IRAN
N
Nasrin
Zahmatkeshan
Nursing department ,School of nursing and midwifery, Yasuj university of medical sciences, Yasuj, IRAN
N
Razeah
Bagherzadeh
Midwifery department, School of nursing and midwifery, Bushehr university of medical sciences, Bushehr, IRAN
N
Kamran
Mirzaei
Department of community medicine, School of medicine, Bushehr university of medical sciences, Bushehr, IRAN
N
Tayebeh
Gharibi
Midwifery department, School of nursing and midwifery, Bushehr university of medical sciences, Bushehr, IRAN
N
Mohammad reza
Yazdankhah
Nursing department ,School of nursing and midwifery, Boushehr university of medical sciences, Boushehr, IRAN
N
Farahnaz
Kamali
Midwifery department, School of nursing and midwifery, Bushehr university of medical sciences, Bushehr, IRAN
N
Rozita
Khoramroodi
Midwifery department, School of nursing and midwifery, Bushehr university of medical sciences, Bushehr, IRAN
N
Shahnaz
Pouladi
Nursing department ,School of nursing and midwifery, Boushehr university of medical sciences, Boushehr, IRAN
N
Background: The world health organization in 2001 established the policy that neonates must be exclusively breastfed for six months ,but studies have shown that fewer women upper than three months to give their babies milk. Although, education and management for a logical continuation of breast feeding is crucial. The present study compared the effectiveness of multimedia and routine educations on exclusive breast feeding in children less than six months of exclusive breast feeding was done.
Materials and Methods: In this experimental study, 169 Gravid 1 women covered by health care systems were chosen via random sampling casually in the control and case groups (77 and 92 individuals, respectively). The intervention group completed 32 weeks of gestation to 6 months after delivery through the implementation of multimedia training programs about breast feeding (slide, educational videos, lecturing and pamphlets) were used for training and advice. While the control group was taught by conventional health centers (providing user instruction). Information required by valid and reliable questionnaires were collected. The data collected by SPSS 11.5 software using descriptive statistics (mean, SD)and analytical statistics (mann Whitney and χ2) were analyzed. In all cases p<0.05 was considered significant.
Results: The results showed in no significant differences between the two groups in terms of duration of exclusive breast feeding in follow up visits. But in the intervention group, breast feeding difficulties such as insufficient milk, filled milky, wrong position and breast problems such as breast sore and mastitis were significantly reduced compared to the control group (p<0.001).
Conclusion: Education provided by health centers in Bushehr as well as multimedia training on exclusive breast feeding was effective however, multimedia education in reducing common problems of breast feeding educationis preferred which is better for health centers to train more emphasis on correction position for breast feeding.
multimedia education, current education, exclusive breast feeding
http://ismj.bpums.ac.ir/article-1-641-en.html
http://ismj.bpums.ac.ir/article-1-641-en.pdf