Bushehr University of Medical Sciences
Iranian South Medical Journal
1735-4374
18
6
2016
1
1
Rapid detection of mutations in codon 43 of rpsL gene by RFLP method with BsajI and MbooII restriction enzymes associated with resistance to Streptomycin in clinical isolates of Mycobacterium tuberculosis
1115
1123
FA
Mohammad
Arjomandzadegan
Department of Microbiology and Immunology, Infectious Disease Research Center (IDRC), Arak University of Medical Sciences, Arak, Iran
Somaeah
Geravand
Department of Microbiology, Islamic Azad University, Tehran (Markazi), Iran
Azam
Ahmadi
Department of molecular genetics, Tarbiat modares University, Infectious Disease Research Center (IDRC), Arak University of Medical Sciences, Arak, Iran
Maryam
Sadrnia
Department of Biology, Payame Noor University, Tehran, Iran
Manezheh
Kahbazi
Department of Pediatrics, Infectious Disease Research Center (IDRC), Arak University of Medical Sciences, Arak, Iran
Background: Streptomycin is one of the most efficient treatments of tuberculosis that increasingly reported its drug resistance. The most common mutations associated with drug resistance to streptomycin of Mycobacterium Tuberculosis, causes tuberculosis, and occurs at codons 43 and 88 of rpsL gene. The purpose of this study is study of alterations in rpsL gene with two restriction enzymes BsajI and MbooII related to drug resistant to streptomycin in clinical isolates of mycobacterium tuberculosis.
Materials and Methods: This study was performed using 71 clinical isolates of Mycobacterium tuberculosis. Molecular PCR-RFLP methods were designed with two enzymes BsajI and MbooII for mutation analysis at codon 43 of rpsL, in resistant and susceptible strains, respectively. Finally, the results were compared with sequencing and phenotype strains.
Results: 25 subjects were studied with enzyme BsajI. This enzyme is capable of detection 64 percent of resistant and all sensitive strains. 46 strains were examined by enzyme MboII. This enzyme detected almost 91 percent of sensitive strains. MboII is selected for detection of sensitive strains (unlike BsajI). Results of sequencing rpsL genes in investigated strains, showed fully consistent with the results of PCR-RFLP and proved mutation in codon of 43 of thin genes in studied strains.
Conclusion: The results showed that the PCR-RFLP with designed restriction enzymes can be used as a rapid, simple assay, and has high sensitivity for differentiation of Mycobacterium tuberculosis strains that are resistance to Streptomycin.
Bushehr University of Medical Sciences
Iranian South Medical Journal
1735-4374
18
6
2016
1
1
Assessment the effect of NO inhibition on hippocampal normetanephrine level in stress and non-stress conditions in adult male rats
1124
1131
FA
Hana
Molahoveizeh
Department of Biology, Faculty of Science, Shahid Chamran University, Ahvaz, Iran
Homan
Eshagh Harooni
Department of Biology, Faculty of Science, Shahid Chamran University, Ahvaz, Iran
Hosean
Najafzadeh varzi
Department of Pharmacology, Faculty of Veterinary Medicine, Shahid Chamran University, Ahvaz, Iran
Ahmad Ali
Moazedi
Department of Biology, Faculty of Science, Shahid Chamran University, Ahvaz, Iran
Background: Nitric oxide (NO) has a role in the regulation of neurotransmitters release such as norepinephrine, in the hippocampus.Normetanephrine (NMN) is a metabolite of norepinephrine created by action of catechol-O-methyl transferase (COMT) on norepinephrine. Several studies have shown that various stresses increased release of norepinephrine and its metabolites. Therefore in the present study, the role of Nitric oxide in regulation of norepinephrine release and its metabolism was investigated by administration of L-NAME (NO synthase inhibitor) in stressed and non-stressed rats.
Materials and Methods: For this purpose, 50 adult rats were divided into 10 groups, of which 5 groups were exposed to restraint stress while another 5 groups were without stress. These two set of groups included intact, saline and L-NAME (20, 40, 80 mg/kg). Thirty minutes after intraperituneal injection of L-NAME, brains removed, the hippocampus dissected, weighed, homogenized and centrifuged then amount of NMN measured by ELISA kit.
Results: The results showed that in non-stressed condition amount of NMN were significantly increased in group that received L-NAME (80 mg/kg) in comparison with other groups but in stress condition, amount of NMN was significantly decreased in groups that received L-NAME (20,40,80 mg/kg), in comparison with control and saline groups. Comparison between stress and non-stressed groups showed that stress alone cause an increase in amount of NMN in control and saline groups.
Conclusion: In conclusion, NO synthesis inhibition produced opposite responses with respect to NMN amount in the presence or absence of stress, and probably L-NAME preventing the effect of stress on increasing NMN levels mediated by nitrergic pathway.
Bushehr University of Medical Sciences
Iranian South Medical Journal
1735-4374
18
6
2016
1
1
Study of Fas 1377 G˃A polymorphism in breast cancer of Iranian patients
1132
1139
FA
Zahra
Tahmasbifard
Department of Molecular and Cell Biology, Advanced sciences ,Islamic Azad University, Tehran Medical Sciences Branch. Tehran, Iran
Mandana
Hasanzad
Medical Genomics Research Center, Tehran Medical Sciences Branch, Islamic Azad University, Tehran, Iran
Nahid
Nafisi
Oncoplastic Breast Surgery , Cancer Research Center, Shahidbeheshti University of Medical Sciences, Tehran, Iran
Background: Apoptosis or programmed cell death is essential for developing and tissue repair. Apoptosis stimulates by binding fas to fas ligand that plays an important role in regulation of the immune system. There are Conflicting data on the association between 1377 polymorphisms and susceptibility to cancer. This study has been conducted to investigation the relationship between polymorphism 1377 A / G in the Fas gene and breast cancer of Iranian patients.
Materials and Methods: 65 patients with breast cancer and 57 control subjects were studied. In this study, PCR-RFLP method was used to determine genotypes. Statistical analysis software SPSS 19, by two-dimensional tables X2 test with 99% confidence intervals were calculated .
Results: The results were showed that the genotype AA 70.7%, GG 27.7%, AG 1.53% of breast carcinoma samples and genotypes AA 56.1%, GG 17.5%, AG 26.3% prevalence among the controls. Also according to Hardy-Weinberg equilibrium, the frequency of allele A in cancer patients was 71.5% and the frequency of G was 28.5% and the frequency of allele A in control subjects was 69.3% and for allele G 30.7% was calculated. Statistically significant relationship was observed between the two groups. (P-Value ˃0.01)
Conclusion: According to the findings of this study, polymorphism 1377 G / A in Fas gene were associated with susceptibility to breast cancer, and it can be considered as a factor in breast carcinogenesis.
Bushehr University of Medical Sciences
Iranian South Medical Journal
1735-4374
18
6
2016
1
1
Cloning and sequencing of the gene encoding LipL21 in the vaccinal leptospira serovars
1140
1148
FA
Rasoul
Hoseinpur
Leptospira Reference laboratory, Department of Microbiology, Razi Vaccine Research Institute, Karaj, Iran
Pezhvak
Khaki
Leptospira Reference laboratory, Department of Microbiology, Razi Vaccine Research Institute, Karaj, Iran
Soheila
Moradi Bidhendi
Leptospira Reference laboratory, Department of Microbiology, Razi Vaccine Research Institute, Karaj, Iran
Mojtaba
Noofeli
Human Bacterial Vaccines Research and Production Department, Razi Vaccine Research Institute, Karaj, Iran
Background: Leptospirosis is a zoonotic disease in humans and animals, caused by the bacterium Leptospira interrogans. Gene expressing LipL21 is one of the genes identified in the bacterium, existing only in the pathogenic strains. The aim of this study was to cloning and analyzing the sequence of the gene encoding surface lipoprotein, LipL21, in five vaccinal leptospira serovars in Iran.
Material and Methods: Pathogenic Leptospira interrogans serovars were cultured in EMJH medium with 10% rabbit serum. After genomic DNA extraction, PCR with specific primers was employed and the resulting product inserted in a vector then transferred into E. Coli DH5&alpha. The recombinant plasmids were finally sent for sequencing.
Results: The analysis of gene lipL21 in domestic vaccinal serovars and comparison of them with other serovars in the GenBank database revealed that three vaccinal serovars serjo hardjo, canicola and pomona had 100% similarity with each other and grippotyphosa serovar had the highest difference with the vaccinal serovars. In general, the results showed that this gene is a highly conserved gene in the domestic vaccinal serovars and serovars in the GenBank database with more than 95.7 percent similarity.
Conclusion: These results showed that the gene, lipL21, is highly conserved in the vaccinal serovars (similarities > 96.4 %). Therefore, the gene encoding surface protein LipL21 can serve as a useful serologic test with high specificity and sensitivity for diagnosis of leptospirosis in clinical samples and in future as an effective subunit vaccine candidate to be used.
Bushehr University of Medical Sciences
Iranian South Medical Journal
1735-4374
18
6
2016
1
1
Effect of nitroprusside on furosemide-induced skeletal teratogenicity in rat fetuses
1149
1163
FA
Mahmood
Khaksary Mahabady
Department of Anatomy and Embryology, Faculty of Veterinary Medicine, ShahidChamran University, Ahvaz, Iran
Hosean
Najafzadeh Varzi
Department of Pharmacology, Faculty of Veterinary Medicine, ShahidChamran University, Ahvaz, Iran
Mozhgan
Sabet
Faculty of Veterinary Medicine, Shahid Chamran University, Ahvaz, Iran
Background: Furosemide as a loop diuretic can use in treatment of hypertension, renal or heart failures and cirrhosis, when sodium retention is significant. It is known that use of furosemide can be lead congenital abnormalities in humans and animals. Nitroprusside as a NO donor can decrease blood supply complications and constriction of placenta and uterus via vasodilation and improvment blood supply. The aim of this study was preventation or decrease of teratogenicity form furosemide in rat fetuses by sodium nitroprusside.
Materials and Methods: This study was performed on 28 pregnant rats that were divided into four groups, the groups consist control, furosemide, sodium nitroprusside and furosemide plus sodium nitroprusside. Drugs were administrated on 14th and 16th day of gestation. Test groups received furosemide (200mg/kg) orally, and nitroprusside (0.5 mg/kg) intraperitoneally. The rats were euthanized and fetuses were collected at 19th day of gestation, after weight and length determination, they stained by Alizarin red- Alician blue method. Then the skeletal system of the stained fetuses was investigated by stereomicroscope for teratogenicity effects.
Results: The results showed the cleft palate, wavy ribs and decreased ossification mean incidence in forelimbs and hindlimbs were 11.11%, 68.88% and 20% in the fetuses of the rats received furosemide, where as it decreased to 7.31%, 21.95% and 12.19% in group which received furosemide plus nitroprusside, respectively.
Conclusion: It is concluded that sodium nitroprusside can significantly decrease teratogenicity induced by furosemide.
Bushehr University of Medical Sciences
Iranian South Medical Journal
1735-4374
18
6
2016
1
1
A Comparison the Hemodynamic Changes between Cutaneous and Intravenous Nitroglycerin in Patients with Known Ischemic Heart Disease under Phacoemulsification Surgery
1164
1170
FA
Abdolrasool
Anvaripour
Departmen of Anesthesia and Critical Care, Bushehr University of Medical Sciences, Bushehr, Iran
Lila
Kazemi Zanjani
Departmentof Ophthalmology, Bushehr University of Medical Sciences, Bushehr, Iran
Abdolreza
Najafi Anaraki
Departmen of Anesthesia and Critical Care, Bushehr University of Medical Sciences, Bushehr, Iran
Kamran
Mirzaei
Department of Community Medicine, Bushehr University of Medical Sciences, Bushehr, Iran
Background: In order to provide better hemodynamic control in patients with known ischemic heart disase in phacoemulsification surgery under general anesthesia. In this study we compared Cutaneous Nitroglycerin and Intravenous Nitroglycerin on hemodynamic stability in this procedure under general anesthesia.
Material and Methods: In this randomized, prospective clinical trial study, 50 patients with 50- 70 years old candidate for phacoemulsification surgery under general anesthesiadivided two groups: 25 patients in control group received intravenous nitroglycerin and 25 ones in case group received cutaneous nitroglycerin. For the intravenous nitroglycerin patients group, before induction of anesthesia, 0.25–0.5 mcg/kg/min intravenous nitroglycerin started and the dose was adjusted according to the hemodynamic changes. All patients underwent cardiac monitoring for arrhythmia detection and S-T segment and T changes during anesthesia by NIBP and ECG monitoringwith a specified time (before induction of anesthesia 1 and 3 minutes after induction after the placement of laryngeal mask after the incision of surgery and after removal of the mask) were recorded.A nitroglycerin skin patch (ointment 2%) of up to 2 cm was applied in the anterior chest wall 40 minutes before the induction of anesthesia.All patients operated with same method in phacoemulsification surgery without epinephrine solution.A statistical analysis was performed with SPSS software (version 16).A p-value less than 0.05 was considered statistically significant.
Results: There were no difference between groups in Mean age and sex, baseline hemodynamic values including: systolic blood pressure, diastolic blood pressure, heart rate, and mean arterial pressure.The systolic blood pressure in the intravenous nitroglycerin group in the third minute after induction of anesthesia, insertion of laryngeal mask, and removal of the mask and one hour after surgery was lower than that of the nitroglycerin skin group this difference was statistically significant.Diastolic blood pressure in the intravenous nitroglycerin group was lower than other groups after removal of the laryngeal mask than that of the nitroglycerin skin group this difference was statistically significant.Heart rates in the intravenous nitroglycerin group at the third minute after induction of anesthesia, insertion of the laryngeal mask, and stimulation surgery were higher than in those of the nitroglycerin skin groups this difference was statistically significant.No statistical significances were discovered in mean arterial blood pressure in both groups. A statistical analysis was performed with SPSS software (version 16).A p-value less than 0.05 was considered statistically significant.
Conclusion: In this study our findings demonstrate that the nitroglycerin skin patch in patients with known ischemic heart disease undergoing phacoemulsification surgery under general anesthesia induces hemodynamic stability. Intraoperative events such as systolic hypertension, diastolic hypotension, and persistent tachycardia does not affect known ischemic patients with cutaneous nitroglycerin.
Bushehr University of Medical Sciences
Iranian South Medical Journal
1735-4374
18
6
2016
1
1
Cytomegalovirus infection in NICU admitted neonates in Boushehr
1171
1178
FA
Maryam
Sanjideh
Department of biology, Kazeroon branch, Islamic Azad University, Kazeroon, Iran
Sirous
Naeimi
Department of biology, Kazeroon branch, Islamic Azad University, Kazeroon, Iran
Maryam
Moradi Nasab
The Persian Gulf Tropical Medicine Research Center, The Persian Gulf Biomedical Research Institute, Bushehr University of Medical Sciences, Bushehr, Iran
Roya
Moradi
The Persian Gulf Tropical Medicine Research Center, The Persian Gulf Biomedical Research Institute, Bushehr University of Medical Sciences, Bushehr, Iran
Katayon
Vahdat
The Persian Gulf Tropical Medicine Research Center, The Persian Gulf Biomedical Research Institute, Bushehr University of Medical Sciences, Bushehr, Iran
Background: Cytomegalovirus is the most prevalent cause of congenital infections and the most important cause of congenital deafness. Which it's spread is about 0.64% of all birth which differ based on geolocation, race and socioeconomically situations. This proposal accomplished in the end of July until middle of February 2014 with the goal of studying Cytomegalovirus infection distribution among newborns who are hospitalized in Bushehr Shohadaye Khalij Fars hospital NICU.
Material & Method: 80 urine samples were collected between July until February 2014 in NICU of Bushehr Khalij Fars hospitalized neonates. Samples were tested by PCR method on urine samples to find if they are infected by cytomegalovirus.
Results: Mean age of neonates was 30.59±9.30 days. Only one newborn under 30 days had Cytomegalovirus and 11 cases older than 30 days had positive reaction. The relation between age and CMV seropositivity was statistically valid (p<0.05).this means only 1.2% of newborns are CMV and 55% are older than 1 month.
Conclusion: The pattern of CMV seropositivity shows that most infections may be acquired from environment. According to low prevalence of congenital CMV infection, there is no need to introduce preventive methods and following present guidelines is enough.
Bushehr University of Medical Sciences
Iranian South Medical Journal
1735-4374
18
6
2016
1
1
The effects of Hyperglycemia and Hyperlipidemia on blood indices
1179
1185
FA
Mohammad
Mahmoudi
Minoodasht Pathobiological Laboratory, Minnodasht, Golestan, Iran
Shaban
Alizadeh
Department of Hematology and Transfusion Medicine, School of Allied Medical Sciences, Tehran University of Medical Sciences, Tehran, Iran
Akbar
Dorgalaleh
Department of Hematology and Transfusion Medicine, School of Allied Medical Sciences, Tehran University of Medical Sciences, Tehran, Iran
Shadi
Tabibian
Allied Medical Student's Scientific Research Center, Tehran University of Medical Sciences, Tehran, Iran
Samira
Esmaeili Reykandeh
Allied Medical Student's Scientific Research Center, Tehran University of Medical Sciences, Tehran, Iran
Morteza
Shamsizadeh
Faculty Member, School of Nursing and Midwifery, Shahroud University of Medical Sciences, Shahroud, Iran
Background: Cell Blood Count (CBC) are performed with automatic analysers in laboratories.It works based on three principles electrical impedance, scatter light and flowcytometry. Many factors might affect results by these machines. This study was performed to assay the effect of some confounders on the results of analysers that work based on electrical resistance.
Material and method: This analytical study (case- control) was conducted on 243 persons with hyperglycemia and hyperlipidemia and 100 healthy persosn (control group) in Fateme Zahra Hospital in North of Iran, Golestan. First, Blood glucose and triglyceride were measured with biochemical analyser and CBC was performed for each person. Finally, Statistical analysis and comparison between two groups were performed with SPSS software.
Resuts: Comparison between case and control group was shown that both hyperlipidemia and hyperglycemia cause increase in Mean Cell Volume (MCV) and also hyperlipidemia can cause increase in Mean Cell Hemoglobin Concentration (MCHC), Hemoglobin and Hematocrit (Hct) with (P<0.05)
Conclusion: According to this study, confounding factors such as hyperglycemia and hyperlipidemia can affect the results of analysers that work based on electrical impedance and it should be considered in laboratories.
Bushehr University of Medical Sciences
Iranian South Medical Journal
1735-4374
18
6
2016
1
1
Evaluation the effects of L-arginine supplementation on exercise performance, body composition and serum sodium and potassium in healthy male athletes
1186
1197
FA
Jahanger
Karimian
Department of Management and Medical Information, Isfahan University of Medical Sciences, Isfahan,Iran
Mohammadhosean
Entezari
Food Security Research Center, Department of Clinical Nutrition, School of Nutrition and Food Science, Isfahan University of Medical Sciences, Isfahan, Iran
Naseh
Pahlavani
Department of Biostatistics, School of Health, Isfahan University of Medical Sciences, Isfahan, Iran
Bahman
Papi
Department of Biostatistics, School of Health, Isfahan University of Medical Sciences, Isfahan, Iran
Hamid
Rasad
Food Security Research Center, Department of Clinical Nutrition, School of Nutrition and Food Science, Isfahan University of Medical Sciences, Isfahan, Iran
Fakhrodin
Chaboksavar
Department of Health Education, School of Health, Isfahan University of Medical Sciences, Isfahan, Iran
Background: L- Arginine is a semi-essential amino acid that can affect athletic performance. Thus the purpose of this study was to evaluate the effect of L- arginine supplementation on athletic performance, body composition and serum sodium and potassium levels in male athletes.
Materials and Methods: This study was a randomized double-blind controlled clinical trial. Participants, 56 male athletes with an average age of 20.85±4.29 years were selected in Isfahan University of Medical Science clubs in the winter of 2014. Athletes received l- arginine supplementation with a dose of 2 g daily for 45 days in the intervention group and the same amount of placebo (maltodextrin) in the control group received. At the beginning and end of the study, the level of athletic performance, body composition and serum sodium and potassium levels were measured and data were analysis with using SPSS software version 19.
Results: At the end of the study athletic performance in the group receiving supplements of L - arginine significantly improved compared to the control group (P=0.035). However, no significant changes in body composition and serum sodium and potassium levels were observed (P>0.05).
Conclusion: Supplementation of L - arginine can improve athletic performance in semi-professional athletes.
Bushehr University of Medical Sciences
Iranian South Medical Journal
1735-4374
18
6
2016
1
1
Molecular Typing of Pseudomonas aeruginosa Strains Isolated from Burn Patients in South of Iran
1198
1207
FA
Aziz
Japoni
Professor Alborzi Clinical Microbiology Research Center, Nemazee Hospital, Shiraz University of Medical Sciences, Shiraz, Iran
Mojtaba
Anvarinejad
Professor Alborzi Clinical Microbiology Research Center, Nemazee Hospital, Shiraz University of Medical Sciences, Shiraz, Iran
Jalal
Mardaneh
Department of Microbiology, School of Medicine, Gonabad University of Medical Sciences, Gonabad, Iran
Background: Pseudomonas aeruginosa is one of the main etiological agents in burn infections which could be life threatening for the infected patients. The aim of the present study was to identify and track source of infections using two molecular typing methods.
Materials and Methods: Seventy-four strains of P. aeruginosa were isolated from burn patients and hospital environment in Ghotbadden Burn Hospital, Shiraz, Iran. Isolates were typed by arbitrary primed-polymerase chain reaction (AP-PCR) and plasmid profiling. Similarity and clustering of the strains was assessed using NTSYS-PC software and photo Capt Mw program.
Results: Thirty eight plasmid profiles were obtained and classified them into: 2, 3and 5 clusters, based on 50%, 64.7% and 67.5% similarity on the plotted dendrogram, respectively. Drawn dendrogarm categorized AP-PCR products to 47 different types.
Conclusion: Based on these results, a limited number of P. aeruginosa types are predominant in the hospitals which infect the burn patients. To control of the infections in patients with antibiotics, resistant isolates, strong disinfection of patients’ bathroom after scrubbing of patients wounds, should be implemented.
Bushehr University of Medical Sciences
Iranian South Medical Journal
1735-4374
18
6
2016
1
1
Assessment of Anti- Inflammatory effect of sea urchin Echinometra mathaei From the Persian Gulf
1208
1220
FA
Solmaz
Soleimani
Department of marine biology, Faculty of Marine Science and technology, Hormozgan University, Bandar Abbas, Iran
Morteza
Yousefzadi
Department of marine biology, Faculty of Marine Science and technology, Hormozgan University, Bandar Abbas, Iran
Sohila
Moein
Department biochemistry & Molecular medicine research center, Faculty of Medical Sciences, Hormozgan University, Bandar Abbas, Iran
Narges
Amrollahi Bioki
Department of marine biology, Faculty of Marine Science and technology, Hormozgan University, Bandar Abbas, Iran
Background: sea urchins belonging to phylum echinoderms of marine invertebrates them found to possess excellent. Inflammation can be considered a set of complex processes that many body systems including the immune and nervous system are involved. The aim of the present research was undertaken to study the anti- inflammatory activity of different extracts, coelomic fluid and pigments shells and spines of sea urchin of Echinodermata mathaei.
Material and method: Isolation of different tissues extracts (spine, shell, gonad and aristotol lantern) sea urchin by three solvents (n- hexan, ethyl acetate, methanol). Isolation coelomic fluid by buffered mode and pigments shell and spine by HCl of sea urchin evaluation antioxidant (DPPH radical scavenging) and anti- inflammatory activity investigated through inhibition albumin serum denaturation.
Resuts: According to the results of the study, the spine- methanoli and coelomic fluid (CF) had the highest activity in the DPPH radical scavenging and aristotol lantern- ethyl acetate and pigment shell highest activity in the anti- inflammatory methods. Significant differences were observed at P<0.05.
Conclusion: The result of this research indicated that sea urchin of E.mathaei due to the high antioxidant activity, have anti- inflammatory activities too.
Bushehr University of Medical Sciences
Iranian South Medical Journal
1735-4374
18
6
2016
1
1
Role of the educational and non-educational factors on the mental health in girl high school students in Bushehr city on 2014
1221
1235
FA
Zohreh
Shakib
Department of Health Education and Health Promotion, Student Research Committee, Bushehr University of Medical Sciences, Bushehr, Iran
Azita
Noroozi
The Persian Gulf Tropical Medicine Research Center, The Persian Gulf Biomedical Research Institute, Bushehr University of Medical Sciences, Bushehr, Iran<br> Department of Health Education, School of Health, Bushehr University of Medical Sciences, Bushehr, Iran
: The mental health has a prominent role in psychosocial development in different periods of life, especially during adolescence. Mental disorders in adolescents can be related to different educational and non-educational factors. Therefore the aim of this study was to identify the educational and non-educational factors affecting the mental health of femal high school students in Bushehr.
Materials and Methods: In this cross sectional study, 500 high school femal students evaluated with using multi-stage cluster random sampling. Information was collected by questionnaire with three-part including educational factors (suchas anxiety academic and educational motivation), non educational factors (suchas demographic characteristics) and quality of life (SF-36) part. The data were analyzed with SPSS software by using appropriate statistical tests.
In this study the mental health level was moderate (50.99±11), and mental health was associated with educational motivation, facilitator anxiety, the school years, educational branch, interest to educational branch, mother education, evaluation of educational counseling, and evaluation of non educational counseling. Maternal education, interest to educational branch, the evaluation of non-educational counseling, facilitating stress, the school year, and educational branch were predictors of mental health (R2= 0.107).
According to the results, providing educational consulting for increasing interest to educational branch, also non educational consulting for reducing problems and designing stress management workshop are necessary to improve students' mental health.
Bushehr University of Medical Sciences
Iranian South Medical Journal
1735-4374
18
6
2016
1
1
Investigation of Hemodialysis Adequacy in Patients Undergoing Hemodialysis in the Shohada Hospital in Bushehr
1236
1244
FA
Zinab
Ghorbane Moghaddam
Department of Nursing, School of Nursing and Midwifery, Bushehr University of Medical Sciences, Bushehr, Iran
Sharif
Sharifi
Department of Nursing, School of Nursing and Midwifery, Bushehr University of Medical Sciences, Bushehr, Iran
Alireza
Raeese
Department of Internal Medicine, Faculty of Medicine, Bushehr University of Medical Sciences, Bushehr, Iran
Mohammadreza
Yazdankhah Fard
Department of Nursing, School of Nursing and Midwifery, Bushehr University of Medical Sciences, Bushehr, Iran
Kamran
Mirzaee
Department of Social Medicine, Faculty of Medicine, Bushehr University of Medical Sciences, Bushehr, Iran
Masoud
Bahraine
Department of Nursing, School of Nursing and Midwifery, Bushehr University of Medical Sciences, Bushehr, Iran
Background: In daily clinical practice two useful tools for calculation of hemodialysis adequacy are Urea Kinetic Modeling and Urea Reduction Ratio (URR). The amount of dialysis that a patient receives can impact morbidity and mortality. The main goal of this research is investigating of dialysis adequacy in patients undergoing dialysis in hemodialysis ward.
Material and Methods: This descriptive study was performed on 93 patients admitted in hemodialysis ward.Pre- and post-dialysis blood samples for measurement of BUN levels were obtained according to 2006 National Kidney Foundation guidelines.kt/v Daugirdas formula and URR were used to assess the adequacy of hemodialysis. Data was analyzed by parametric and non parametric tests using the spss version 19 software.
Results: The results of the study show that 59.1 % patients were kt/v &rsaquo1.2 and 53.8% of them were URR&rsaquo 65%. The mean of kt/v and URR were 1.35±0.53 and 66.15%±12.48 respectively .The minimum and maximum kt/v indices were 0.20 and 4.02 and The minimum and maximum URR were 11.50% and 95.10% respectively.
Conclusion: according to the dialysis adequacy indices (kt/v and URR) more than 40% of patients admitted to this Hemodialysis ward received inadequate hemodialysis services that need more supervision and follow up.
Bushehr University of Medical Sciences
Iranian South Medical Journal
1735-4374
18
6
2016
1
1
Investigating health literacy Level of patients referred to Bushehr hospitals and recognizing its effective factors
1245
1253
FA
Abdolrasool
Khosravi
Department of Library and Information Science Medicine, Faculty of Paramedical, Bushehr University of Medical Sciences, Iran
Khadejeh
Ahmadzadeh
Health Information Management Research Center, Hormozgan University of Medical Sciences, Bandar Abbas, Iran
Background: Health literacy is defined as the degree of Individual's capacity to obtain, process, and understand basic health information and services needed to make appropriate health decisions. Functional health literacy is critically important during the typical patient–provider encounter According to this, current research aimed to investigate the level of the health literacy of patients referred to Bushehr’s hospital (Iran) and recognizing its effective factors.
Material and Methods: In this descriptive survey, 250 patients refers to Bushehr’s hospital (Iran) were selected as a sample study. Health literacy was measured by Normalized Test of Functional Health Literacy in Adults. Data were analyzed using SPSS and Independent t-tests, One Way ANOVA and Pearson correlation at a significant level (p.value>0.05).
Results: The findings showed that the level of the health literacy of these patients was in marginal level (69.2). Health literacy was associated by sexuality, age and education.
Conclusion: Generally, this research shows that the health literacy level is not adequate among patients. This result determines it is important to that notice health literacy and trying to improve it. Providing easy and readable educational resources for patients, educational classes for them and so on are some ways to improve health literacy.
Bushehr University of Medical Sciences
Iranian South Medical Journal
1735-4374
18
6
2016
1
1
Halitosis and related factors in patient referred to the Clinic of Dentistry, Zahedan
1254
1261
FA
Tahereh
Nosratzehi
Department of Oral Medicine Dentistry, Dental Research Center, Zahedan University of Medical Science, Zahedan, Iran
Simean
Lesan
Department of Oral Medicine Dentistry, Islamic Azad University of Medical Sciences, Tehran, Iran
Mohammadali
Ousia
Department of Oral Medicine Dentistry, Shahed University of Medical Sciences, Tehran, Iran
Background: Halitosis is defined as unpleasant odor from the mouth .In %87 of cases the cause of halitosis are located in the oral cavity. It is a medico-social problem that affect a many number of people. purpose of this study was to determine factors involved in halitosis patients were referred to the dental clinic Zahedan.
Materials and Methods : 320 people were attending the halitosis or halitophobia for this survey were selected . Selected individuals from eating, drinking, smoking, chewing gum, about 24 hours before sampling was forbidden , and also noted that on examination of brushing, flossing and mouthwash brands do not use . Regarding gingival index periodontal used, in terms of smoking were divided into two groups, consumer and non- consumer - reviews decay and bad restorations through clinical examination and bitewing radiography was performed and the diagnosis of patients with the prosthesis or any the corresponding table were recorded . Crowding teeth were considered . Organoleptic method was used to check for bad breath .
Results: The findings of factors such as tooth decay (72%), periodontal disease (47.5%), tounge coating (17.5%) are artificial teeth (13.75%) and food impaction, were signficantly related with halitosis.
Conclusion: diagnosis and treatment of halitosis are pramily the responsibility of dentists.
It is important for the dentist to have a proper understanding of halitosis and provide correct treatment.
Bushehr University of Medical Sciences
Iranian South Medical Journal
1735-4374
18
6
2016
1
1
Evaluation of in vitro leishmanicidal activity of tea tree oil (Melaleuca alternifolia)
1262
1269
FA
Moradali
Fouladvand
Department of Microbiology and Parasitology, School of Medicine, Bushehr University of Medical Sciences, Bushehr, Iran
Soliman
Khorami
Department of Laboratory Sciences, School of Paramedicine, Bushehr University of Medical Sciences, Bushehr, Iran
Behrooz
Naeimi
Department of Microbiology and Parasitology, School of Medicine, Bushehr University of Medical Sciences, Bushehr, Iran
Background: Leishmania is a trypanosomatid protozoan which is transmitted by the female Phlebotomus sand fly. Leishmaniasis is prevalent in four continents and is considered to be endemic in 88 countries, 72 of which are developing countries. It is estimated that it’s global prevalence and incidence is 12 millions and 500 thousands respectively. The recommended drugs used for the treatment of this disease are mainly ineffective, toxic and exhibit many side effects. Based on some studies,tea tree oil that is a yellow color liquid with spicy odor exhibit antibacterial and antifungal activity, so, we evaluated it’s anti-leishmanial effect.
Materials and Methods: Tea tree oil was purchased from Dr Jahangiri pharmaceutical company and it’s various concentrations (mg/ml800, mg/ml400, mg/ml200, mg/ml100) were prepared in 1ml volume of TSB solvent in 15ml test tubes, then 9 ml of RPMI-1640 medium and 1×106 promastigotes was added to each tube, as a final volume of 10 ml. These tubes were incubated at 23o c for 72 hours and number of parasites in the first 6 hours once every hour and after that every 24 hours were counted by hemocytometer.
Results: Lethal dose of tea tree oil for 50% of promastigotes ( IC50 ) is 337 mg/ml, while the 800 mg/ml of this extract can killed 95% of the parasites after 24 hours of incubation, and it’s lower concentrations of this extract showed dose-dependent leishmanicidal activity. Glucantime as positive control drug at concentration of 300 mg/ml killed all the promastigotes, while the number of promastigotes in negative control tubes was increase during the incubation period.
Conclusion: Anti-Leishmanial activity of tea tree oil with IC50 of 337 mg/ml and 95% lethality of 800 mg/ml is promising and further study for analyse the active ingredients of this oil and evaluation of it’s anti-leishmanial effect, particularly in laboratory animal is recommended.
Bushehr University of Medical Sciences
Iranian South Medical Journal
1735-4374
18
6
2016
1
1
Bryozoans, the remedy hidden treasures of oceans: secondary metabolites
1270
1286
FA
Gholamhosean
Mohebbi
The Persian Gulf Marine Biotechnology Research Center, The Persian Gulf Biomedical Research Institute, Bushehr University of Medical Sciences, Bushehr, Iran
Iraj
Nabipour
The Persian Gulf Marine Biotechnology Research Center, The Persian Gulf Biomedical Research Institute, Bushehr University of Medical Sciences, Bushehr, Iran
Seyedeh Samira
Arshadi
The Persian Gulf Marine Biotechnology Research Center, The Persian Gulf Biomedical Research Institute, Bushehr University of Medical Sciences, Bushehr, Iran
Bryozoans, commonly known as “moss animals”, are typically aquatic, filter feeding, sessile, colonial marine animals. Although, they are a rich resource of new bioactive secondary metabolites, but studies on their secondary metabolite have been neglected. Although over 8000 species are known, the lowest numbers of novel compounds were isolated from the phylum Bryozoa. In addition, presently nothing is recognized regarding the origin of the bioactive compounds isolated by bryozoans. At present nothing is known regarding the origin of the natural products isolated by bryozoans. The most famous of compounds obtained from these organisms are macrolide lactones of Bryostatins. They are potent modulators of protein kinase C. The amazing compounds such as Flustramines, Hinckdentine, Convolutamines, Kororamides, Chatellines, Chartellamides, Convolutamines, Convolutamidines, euthyroideone, amathaspiramide, lutamides, Volutamides, tambjamines, Phidolopins, and dozens of other compounds with different biological properties have been obtained. Very few studies have been done on these treasures in the sea depth, and more studies need to be done on them for access to their secondary metabolites.
Bushehr University of Medical Sciences
Iranian South Medical Journal
1735-4374
18
6
2016
1
1
The technological singularity and exponential medicine
1287
1298
FA
Iraj
Nabipour
Future Studies Group, The Academy of Medical Sciences of the I.R.Iran<br> The Persian Gulf Marine Biotechnology Research Center, The Persian Gulf Biomedical Research Institute, Bushehr University of Medical Sciences, Bushehr, Iran
Majid
Assadi
The Persian Gulf Nuclear Medicine Research Center, The Persian Gulf Biomedical Research Institute, Bushehr University of Medical Sciences, Bushehr, Iran
The "technological singularity" is forecasted to occur in 2045. It is a point when non-biological intelligence becomes more intelligent than humans and each generation of intelligent machines re-designs itself smarter. Beyond this point, there is a symbiosis between machines and humans. This co-existence will produce incredible impacts on medicine that its sparkles could be seen in healthcare industry and the future medicine since 2025. Ray Kurzweil, the great futurist, suggested that three revolutions in science and technology consisting genetic and molecular science, nanotechnology, and robotic (artificial intelligence) provided an exponential growth rate for medicine. The "exponential medicine" is going to create more disruptive technologies in healthcare industry. The exponential medicine shifts the paradigm of medical philosophy and produces significant impacts on the healthcare system and patient-physician relationship.