2024-03-28T12:38:24+03:30 http://ismj.bpums.ac.ir/browse.php?mag_id=73&slc_lang=fa&sid=1
73-1290 2024-03-28 10.1002
Iranian South Medical Journal Iran South Med J 1735-4374 1735-6954 10.52547/ismj 2020 23 2 Molecular Typing of Mycobacterium Tuberculosis Isolated from Iranian Patients Using Highly Abundant Polymorphic GC-Rich-Repetitive Sequence Bahram Golestani Eimani Khalil Ansarin Leila Sahebi Maryam Seyyedi seyyedim@tbzmed.ac.ir Background: Tuberculosis (TB) with more than 10 million new cases per year and one of the top 10 causes of death worldwide, is still one of the most important global health problems. Also, multi drug-resistant tuberculosis (MDR) is a serious danger to public health. Understanding of the epidemiological pattern of mycobacterium tuberculosis (MTB), Estimates of recent transmission and recurrence of infection, it is possible with molecular typing methods. The present study was performed aiming to track and determine the type of Mycobacterium tuberculosis infection, as well as its relationship with demographic factors, using PGRS-RFLP. Materials and Methods: In this study, 90 samples collected from TB patients from the North West provinces of Iran, Molecular typing by Characterization highly abundant polymorphic GC-rich-repetitive sequence. Investigated Demographic factors associated with the transmission of the disease. Results: All isolates were grouped into 44 clusters 28types (33.3%) of the subspecies were in unique stains and 66.7% (from 56 patients) had clustered isolates. The largest cluster contained 8 isolates (9.52%) was the West provinces of Iran. Conclusion: Genetic variation of Mycobacterium tuberculosis is high in this region. The rate of recent transmission based on clustering was unexpected (The global average is 30%-40%). The recent transmission was more dynamic in the west than northwest Iran. Clustering based on PGRS-RFLP can demonstrate the high correlation between molecular and classic information. In addition, the significant relationship between vaccination record and clustering highlights the necessity to conduct more extensive studies. Mycobacterium tuberculosis PGRS-RFLP Molecular typing Epidemiology 2020 6 01 87 98 http://ismj.bpums.ac.ir/article-1-1290-en.pdf 10.52547/ismj.23.2.87
73-1291 2024-03-28 10.1002
Iranian South Medical Journal Iran South Med J 1735-4374 1735-6954 10.52547/ismj 2020 23 2 Magnetic Separation of Giardia Cysts from Water Reza Ghasemikhah Zainab Davudi Moghadam Fatemeh Ahmadi Mahsa Rasulpur Mohammad Reza Bayatiani Reza Hajihossein Hossein Sarmadian Fatemeh Seif seif@arakmu.ac.ir Background: Giardia is one of the infectious agents that can be transmitted by surface water and cause a disease. We designed and fabricated a magnetic system to separate the giardia cysts from water. Materials and Methods: In this study, giardia cysts were concentrated by sucrose floatation method. The cysts were mixed with physiological saline and their concentration was adjusted to 100,000 cysts per cubic millimeter using Neubauer slide. The isolated cysts were passed through the magnetic field generated by the 200-msT magnets. The cysts were attracted to the magnet and removed from the suspension. The cysts were exposed to magnetic field for one week in order to evaluate the effect of magnetic field on their survival, which was reported as 0.1% using eosin staining under a light microscope. Results: The results showed that magnetic field can relatively absorb giardia cysts from water, the survival rate of the cysts did not change significantly in different days (P≥0.05). Conclusion: The designed magnetic system can be reinforced to isolate giardia cysts, which is economical and has no chemical effects on water. Giardia cyst separation Sucrose Magnetic field 2020 6 01 99 107 http://ismj.bpums.ac.ir/article-1-1291-en.pdf 10.52547/ismj.23.2.99
73-1292 2024-03-28 10.1002
Iranian South Medical Journal Iran South Med J 1735-4374 1735-6954 10.52547/ismj 2020 23 2 Qualitaty of Olfaction in Patients with Nasal and Sinuses Polyposis before and after Endo-scopic Sinus Surgery in, Vali e Asr Hospital in 2017 Masoud Asghari masasghari58@gmail.com Mehdi Bakhshai Background: Olfaction plays an important role in human life and promotes quality of life through understanding various odors. Nasal polyps are among the common causes of olfactory impairment. There are many different treatment options for polyps most notably the diagnosis and elimination of underlying causes. Some polyps do not respond to medication therapy or they relapse, and therefore require surgery. The present study aimed to determine olfactory function in patients with sinus and nasal polyposis before and after endoscopy. Materials and Methods: In this cross-sectional study, patients admitted to, Vali e Asr Hospital of Birjand in 2015 for endoscopic nasal polypectomy were included. Evaluation of olfactory quality was performed prior to and four weeks after the surgery. Olfactory function was evaluated by a semi-structured questionnaire on the quality of olfactory, taste and appetite combined with the Quick Odor Detection test. Data was analyzed in SPSS 23 software using descriptive statistics of frequency, perentage, mean and standard deviation, and analytical statistics of McNemar’s and Wilcoxon tests. P value of <0.05 was considered significant. Results: Thirty-six patients were enrolled. All patients underwent endoscopic polypectomy. Their mean age was 39.14±11.69 years. Most of them were male (n=21, 58.3%). History of nasal polyps, asthma, allergic rhinitis and aspirin sensitivity was positive in 27.8%, 11.1%, 47.2% and 27.8% of patients. Nasal polyps were unilateral in 58.3% of patients and bilateral in 41.7% of patients. There was a significant improvement in olfactory quality after surgery (p<0.001). The sense of smell was present in 75% or the patients before and in 100% of them after the surgery, and the difference was statistically significant (p=0.004). Conclusion: Olfactory dysfunction in patients with polyps is remarkably prevalent. Overall, the results of this study show polypectomy significantly improved patients’ sense and quality of smell. Olfactory quality sinus polyposis nasal polyposis endoscopic surgery 2020 6 01 108 115 http://ismj.bpums.ac.ir/article-1-1292-en.pdf 10.52547/ismj.23.2.108
73-1293 2024-03-28 10.1002
Iranian South Medical Journal Iran South Med J 1735-4374 1735-6954 10.52547/ismj 2020 23 2 Predictor Factors of Recycling Behavior in Residents of Bushehr City: Application of Theo-ry of Planned Behavior Sahar Haghighatjoo Aida Asgari Rahim Tahmasebi Azita Noroozi azitanoroozi@yahoo.com Background: Recycling is one of the most important measures to manage the municipal solid waste. In order to design a training program, it is necessary to identify the factors that influence behavior. Therefore, the present study aimed to investigate the predictive factors of recycling behavior among residents of Bushehr city based on the extended theory of planned behavior.  Materials and Methods: In this cross-sectional study, 582 people were selected through convenience sampling method. A questionnaire with items on demographic characteristics, extended theory of planned behavior, and recycling behavior was used for data collection. The collected data were analyzed by SPSS software version 20. Results: In this study, 221 participants (38%) adhered to recycling behavior. There was a significant relationship between knowledge of waste collection program (P<0.001), receiving recycling incentive packages (p=0.003), and recycling waste disposal vehicles (p= 0/001) with recycling behavior. Past behavior (OR=0.229, P<0.001), and descriptive norms (OR=1.187, P=0.003) were predictive of recycling behavior.  Conclusion: The results of the present study indicate that regular refuse collection vehicles, providing environmental facilities including incentive packages, and informing citizens about the schedule of refuse collection vehicles can lead to recycling behavior. Also, friends, relatives, and family are role models for other members by establishing norms and are effective in promoting recycling behavior. Having a positive experience of a behavior is necessary for its maintenance. Recycling Attitude Behavior change Intention. 2020 6 01 116 128 http://ismj.bpums.ac.ir/article-1-1293-en.pdf 10.52547/ismj.23.2.116
73-1294 2024-03-28 10.1002
Iranian South Medical Journal Iran South Med J 1735-4374 1735-6954 10.52547/ismj 2020 23 2 Major Dietary Patterns of Women Living in Urban and Rural Areas of Dashtestan County (Bushehr Province), 2017 Arezoo Rezazadeh arezoo.rezazadeh@gmail.com Razeah Hassanpour Bahram Rashidkhani Background: The pattern of dietary intake reflects the preferences and dietary habits of individuals and their socio-economic and demographic status such as ethnicity and place of residence. So far, various studies have examined the dietary patterns of different populations in Iran, but the information on eating patterns of rural areas is limited in comparison with urban regions. The aim of this study was to identify major dietary patterns and to compare the scores of these patterns in women living in urban and rural areas of Dashtestan County, (Bushehr province), 2017. Materials and Methods: In this cross-sectional study, 302 women (168 urban and 134 rural) aged 20_59 years old living in Dashtestan county were sampled with multistage sampling method (stratified cluster sampling). Dietary intake was assessed by filling the standard semi-quantitative food frequency questionnaire in face-to-face interviews. Factor analysis method was used for identifying major dietary patterns. The Mann-Whitney analysis was used to compare the extracted pattern scores in urban and rural women. Results: Three major dietary patterns were extracted: Healthy, western and traditional. The score of western pattern was significantly higher in urban women (p<0.05). The score of other patterns was not significantly different between urban and rural areas. Conclusion: The findings of this study showed that although Dashtestan  county is not a metropolitan area, the rate of compliance with the western food pattern is notable in urban women. Educational programs should be adopted for promoting nutritional status of the study population.   dietary patterns women urban rural Dashtestan. 2020 6 01 129 142 http://ismj.bpums.ac.ir/article-1-1294-en.pdf 10.52547/ismj.23.2.129
73-1295 2024-03-28 10.1002
Iranian South Medical Journal Iran South Med J 1735-4374 1735-6954 10.52547/ismj 2020 23 2 Comparative Evaluation of Expression Vectors (pET32a and pET25b) in Expression of Polyepitopic Sequence Synthesized from Leishmania infantum Antigens Marzeah Taherzadeh MoradAli Fouladvand mfooladvand39@yahoo.com Bahram Kazemi Background: Molecular methods are nowadays used to diagnose diseases, produce vaccines, drugs and recombinant proteins. Therefore, one of the important steps in these procedures is selecting the appropriate vector for cloning and expression of the target genes. Because the expression of synthetic polygenes or so-called chimeric antigens with high molecular weight requires special conditions, the aim of this study was to evaluate the importance of vector type for expression of such antigens. Materials and Methods: The 1250bp sequence consisting of epitopes from eight important antigens of Leishmania infantum parasite was designed and synthesized by Biomatic Company (Cambridge, Canada). The sequence was cloned separately in two expression vectors pET25b and pET32a and then transformed into E.coli BL21 (DE3) and expressed under similar appropriate conditions. Bacterial lysates were analyzed by SDS PAGE and evaluated by Western blotting. Results: of SDS- PAGE and Western blot analysis showed that intact recombinant protein production by pET25b vector was not successful. However, the recombinant protein resulted from the expression of the aforementioned poly-epitope in pET32a vector was successfully produced and confirmed.   Conclusion: According to the successful expression confirmation of the poly-epitopic sequence within the pET32a vector and furthermore the failure to obtain protein in the pET25b vector showed that in the case of some specific mosaic sequences expression, protein isolation will be difficult because of low solubility. So, the expression vector choose should be made more carefully.   Poly-epitopic structure Leishmania infantum Expression level pET25b vector pET32a vector 2020 6 01 143 152 http://ismj.bpums.ac.ir/article-1-1295-en.pdf 10.52547/ismj.23.2.143
73-1296 2024-03-28 10.1002
Iranian South Medical Journal Iran South Med J 1735-4374 1735-6954 10.52547/ismj 2020 23 2 Evaluation of Lethal Effect of Curcumin and its Derivatives Against Leishmania Major In Vitro MoradAli Fouladvand mfooladvand39@yahoo.com Solyman Khorami Behroz Naeimi Sohila Fotouhi Khosro Mohammadi Background: Leishmania major is transmitted by sandflies of the genus Phobotomus and causes cutaneous lesions in humans. Curcumin is made from turmeric and various derivatives is derived from it. In the present study the effect of curcumin and it’s derivatives on Leishmania major in vitro was investigated. Materials and Methods: Curcumin 70% and 90% purity and it’ derivatives such as s , BDMC base methoxy curcumin, diacetyl curcumin DAC, vanadyl curcumin VO (CUR) 2, vanadyl diacetyl curcumin VO (DAC) 2, indium curcumin In(CUR)3  and Ga (CUR) 3 preparation. Curcumin derivatives were synthesized and different concentrations of 500 to 800 μg/ ml were prepared in glycerin. 106 Leishmania major promastigotes added to each well of 96 well plate and 100 µL of each curcumin derivative added and stored at 25 ° C for 24 hours. Assay of the lethality of the compounds against Leishmania major and evaluation of their toxic effects on (vero) cells were evaluated using MTT assay. Results: Lethal effect of curcumin and it’s derivatives against Leishmania, increased with increasing concentration. Anti-leishmanial index (IC50) of 70% and 90% curcumin compounds, (BDMC) bis methoxy curcumin, Diacetyl curcumin (DAC), vanadyl curcumin VO (CUR)2, vanadyl diacetyl curcumin VO (DAC)2, indium curcumin In (CUR)3, Gallium Curcumin Ga (CUR)3 compounds were 110, 93, 101, 103, 98, 103, 51 and 58 µg / ml  and the lethal effect of these compounds against (vero) cells were 33.1%, 19%, 21%, 20.3%, 17%, 21%, 25.3%, 16%, respectively. Conclusion: Gallium curcumin and indium curcumin, compared to curcumin and other derivatives, exhibited the highest anti-leishmanial effect and were the safest derivatives of curcumin for mammalian cells.   Leishmania major Curcumin MTT Vero. 2020 6 01 153 164 http://ismj.bpums.ac.ir/article-1-1296-en.pdf 10.52547/ismj.23.2.153
73-1300 2024-03-28 10.1002
Iranian South Medical Journal Iran South Med J 1735-4374 1735-6954 10.52547/ismj 2020 23 2 The Fifth Generation University: Based on the Quintuple Helix of Carayannis and Campbell Iraj Nabipour inabipour@gmail.com The quintuple helix innovation model has been introduced by E.G. Carayannis and F.J. Campbell. The natural environments of society and the economy are major drivers of this innovation model. The third generation university (the convergence of government, industry and university), the fourth generation universities (the convergence of government, industry, university and civil society) and the fifth generation university (the convergence of government, industry, university, civil society and bio-environment) perform within Triple Helix, Quadruple Helix and Quintuple Helix models, respectively. According to the viewpoints of Carayannis and Campbell, the cardinal characteristics of the fifth generation university could be conceptualized as a knowledge-based innovation system which is sensitive to natural environment of society, produces knowledge within the mode 3 knowledge production framework, supports co-evolution and co-existence of different knowledge paradigms, shapes co-opetitive ecosystem for knowledge management and promotes knowledge democracy. Quintuple helix Innovation University Knowledge Democracy Bioenvironment 2020 6 01 165 194 http://ismj.bpums.ac.ir/article-1-1300-en.pdf 10.52547/ismj.23.2.165