Volume 20, Issue 2 (Iranian South Medical Journal 2017)
Iran South Med J 2017, 20(2): 170-179 |
Back to browse issues page
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Gharibi S, Alebouyeh M, Falsafi T, Farzi N, Vaziri F, Zali M R. Protease Activity of Helicobacter Pylori Strains and their Association with Histopathological Changes of the Gastric Tissue. Iran South Med J 2017; 20 (2) :170-179
URL: http://ismj.bpums.ac.ir/article-1-869-en.html
URL: http://ismj.bpums.ac.ir/article-1-869-en.html
Somaye Gharibi1 
, Massoud Alebouyeh * 2, Tahereh Falsafi1 , Nastaran Farzi3 , Farzam Vaziri4 , Mohammad Reza Zali5
, Massoud Alebouyeh * 2, Tahereh Falsafi1 , Nastaran Farzi3 , Farzam Vaziri4 , Mohammad Reza Zali5
1- Department of Microbiology, School of Biological Science, Alzahra University, Tehran, Iran
2- Foodborne and Waterborne Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran , masoud.alebouyeh@gmail.com
3- Foodborne and Waterborne Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran
4- Mycobacteriology and Pulmonary Research Department, Pasteur Institute of Iran
5- Gastroenterology and Liver Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran
2- Foodborne and Waterborne Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran , masoud.alebouyeh@gmail.com
3- Foodborne and Waterborne Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran
4- Mycobacteriology and Pulmonary Research Department, Pasteur Institute of Iran
5- Gastroenterology and Liver Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Abstract: (5536 Views)
Background: Helicobacter pylori is the main cause of various gastroduodenal diseases. As the diseases caused by H. pylori are associated with severe tissue damage, proteases of bacteria may play an important roles in this process. In current study we investigated diversity of H. pylori protease activity and its association with different pathological lesions of gastric tissue.
Materials & Methods: The study was performed on 116 gastric biopsy specimens obtained from patients with gastrointestinal disorders referred to Taleghani hospital in Tehran, IR.Iran. Isolates were identified by culture and polymerase chain reaction (PCR). Their protease activity was assessed by spectrophotometric and well diffusion assays using casein as substrate. Variance analysis (One-way ANOVA) was used for statistical analysis.
Results: A prevalence of 43.1% (50/116) of H. pylori infection was detected in the studied patients. Histopathological lesions among 50 H. pylori positive patients were included: chronic gastritis 40% (20/50), intestinal metaplasia 8% (4/50), and severe active gastritis 52% (26/50). Nearly, 4.25% of the strains showed high protease activity, while 91.4% and 4.25% of the strains showed moderate and low protease activities, respectively. Statistical analysis demonstrated significant association between low protease activity of the strains and chronic gastritis (p= 0.044).
Conclusion: Results of this study indicated diversity of protease activity among different strains of
H. pylori and significant association between low protease activity of the strains and occurrence of chronic gastritis. Further studies are necessary to investigate this association.
Type of Study: Original |
Subject:
Microbiology and Immunology
Received: 2016/03/2 | Accepted: 2016/07/19 | Published: 2017/04/29
Received: 2016/03/2 | Accepted: 2016/07/19 | Published: 2017/04/29
References
1. Azuma T, Yamakawa A, Yamazaki S, et al. Distinct diversity of the cag pathogenicity island among Helicobacter pylori strains in Japan. J Clin Microbiol 2004; 42(6): 2508-17. [PubMed] [Google Scholar]
2. Ando T, Goto Y, Ishiguro K, et al. The interaction of host genetic factors and Helicobacter pylori infection. Inflammopharmacology 2007; 15(1): 10-4. [PubMed] [Google Scholar]
3. Wroblewski LE, Peek RM. Helicobacter pylori in gastric carcinogenesis: mechanisms. Gastroenterol Clin North Am 2013; 42(2): 285-98. [PubMed] [Google Scholar]
4. Salih BA. The role of the putative virulence markers (cagA and vacA) of Helicobacter pylori in peptic ulcer disease. Saudi Med J 2004; 25(7): 830-6. [PubMed] [Google Scholar]
5. Gerhard M, Lehn N, Neumayer N, et al. Clinical relevance of the Helicobacter pylori gene for blood-group antigen-binding adhesin. Proc Natl Acad Sci U S A 1999; 96(22): 12778-83. [PubMed] [Google Scholar]
6. Yamaoka Y, Ojo O, Fujimoto S, et al. Helicobacter pylori outer membrane proteins and gastroduodenal disease. Gut 2006; 55(6): 775-81. [PubMed] [Google Scholar]
7. Franco AT, Johnston E, Krishna U, et al. Regulation of gastric carcinogenesis by Helicobacter pylori virulence factors. Cancer Res 2008; 68(2): 379-87. [PubMed] [Google Scholar]
8. Abdollahi H, Shokoohi M, Savari M. The prevalence of Helicobacter pylori babA2, iceA1 and iceA2 genes and their association with clinical outcomes in patients with chronic gastritis, ulcerative diseases and non-ulcer dyspepsia in South East of Iran. Jundishapur J Microbiol 2013; 6(4): e4739. [Google Scholar]
9. Shiota S, Suzuki R, Yamaoka Y. The significance of virulence factors in Helicobacter pylori. J Dig Dis 2013; 14(7): 341-9. [PubMed] [Google Scholar]
10. Kidd M, Peek R, Lastovica A, et al. Analysis of iceA genotypes in South African Helicobacter pylori strains and relationship to clinically significant disease. Gut 2001; 49(5): 629-35. [PubMed] [Google Scholar]
11. Peek RM, van Doorn L-J, Donahue JP, et al. Quantitative detection of Helicobacter pylori gene expression in vivo and relationship to gastric pathology. Infect Immun 2000; 68(10): 5488-95. [PubMed] [Google Scholar]
12. Ohnishi N, Yuasa H, Tanaka S, et al. Transgenic expression of Helicobacter pylori CagA induces gastrointestinal and hematopoietic neoplasms in mouse. Proc Natl Acad Sci U S A 2008; 105(3): 1003-8. [PubMed] [Google Scholar]
13. Miyoshi S, Shinoda S. Microbial metalloproteases and pathogenesis. Microbes infect 2000; 2(1): 91-8. [PubMed] [Google Scholar]
14. Frees D, Brøndsted L, Ingmer H. Bacterial proteases and virulence. Subcell Biochem 2013; 66: 161-92. [PubMed] [Google Scholar]
15. Maeda H. Role of microbial proteases in pathogenesis. Microbiol Immunol 1996; 40(10): 685-99. [PubMed] [Google Scholar]
16. Dubin G, Koziel J, Pyrc K, et al. Bacterial Proteases in Disease–role in intracellular survival, evasion of coagulation/fibrinolysis innate defenses, toxicoses and viral infections. Curr Pharm Design 2013; 19(6): 1090-113. [PubMed] [Google Scholar]
17. Löwer M, Weydig C, Metzler D, et al. Prediction of extracellular proteases of the human pathogen Helicobacter pylori reveals proteolytic activity of the Hp1018/19 protein HtrA. PLoS One 2008; 3(10): e3510. [PubMed] [Google Scholar]
18. Wex T, Zack M, Malfertheiner P. Proteases in Helicobacter pylori-Mediated Diseases. In: Lendeckel U, Hooper NM, editors. Proteases in Gastrointestinal Tissues. Netherlands: Springer, 2006, 61-87. [Google Scholar]
19. Tegtmeyer N, Moodley Y, Yamaoka Y, et al. Characterisation of worldwide Helicobacter pylori strains reveals genetic conservation and essentiality of serine protease HtrA. Mol Microbiol 2016; 99(5): 925-44. [PubMed] [Google Scholar]
20. Hoy B, Löwer M, Weydig C, et al. Helicobacter pylori HtrA is a new secreted virulence factor that cleaves E‐cadherin to disrupt intercellular adhesion. EMBO Rep 2010; 11(10): 798-804. [PubMed] [Google Scholar]
21. Loughlin MF, Arandhara V, Okolie C, et al. Helicobacter pylori mutants defective in the clpP ATP-dependant protease and the chaperone clpA display reduced macrophage and murine survival. Microb Pathog 2009; 46(1): 53-7. [PubMed] [Google Scholar]
22. Falsafi T, Khani A, Mahjoub F, et al. Analysis of vacA/cagA genotypes/status in Helicobacter pylori isolates from Iranian children and their association with clinical outcome. Turk J Med Sci 2015; 45(1): 170-7. [PubMed] [Google Scholar]
23. Vaziri F, Najar Peerayeh S, Alebouyeh M, et al. Diversity of Helicobacter pylori genotypes in Iranian patients with different gastroduodenal disorders. World J Gastroenterol 2013; 19(34): 5685-92. [PubMed] [Google Scholar]
24. Yadegar A, Mobarez AM, Alebouyeh M, et al. Clinical relevance of cagL gene and virulence genotypes with disease outcomes in a Helicobacter pylori infected population from Iran. World J Microbiol Biotechnol 2014; 30(9): 2481-90. [PubMed] [Google Scholar]
25. Espinoza MG, Vazquez RG, Mendez IM, et al. Detection of the glmM gene in Helicobacter pylori isolates with a novel primer by PCR. J Clin Microbiol 2011; 49(4): 1650-2. [PubMed] [Google Scholar]
26. Mohapatra BR, Bapuji M, Sree A. Production of industrial enzymes (amylase, carboxymethylcellulase and protease) by bacteria isolated from marine sedentary organisms. Acta Biotechnol 2003; 23(1):75-84. [Google Scholar]
27. Vijayaraghavan P, Prakash Vincent SG. A simple method for the detection of protease activity on agar plates using bromocresolgreen dye. J Biochem Technol 2013; 4(3): 628-30. [Google Scholar]
28. Wang F, Meng W, Wang B, et al. Helicobacter pylori-induced gastric inflammation and gastric cancer. Cancer lett 2014; 345(2): 196-202. [PubMed] [Google Scholar]
29. Falahi J, Bahador A, Motamed N, et al. Prevalence of Helicobacter pylori infection among patients with upper gastrointestinal symptoms in Bushehr, Iran. Iran South Med J 2015; 18(3): 556-66. (Persian) [Google Scholar]
30. Kargar M, Souod N, Ghorbani-Dalini S, et al. Epidemiological evaluation of Helicobacter pylori infection in patients with gastrointestinal disorders in Chahar Mahal and Bakhtiari province. J Fasa Univ Med Sci 2013; 2(4): 266-72. (persian) [Google Scholar]
31. Nilius M, Pugliese M, Malfertheiner P. Helicobacter pylori and proteolytic activity. Eur J Clin invest 1996; 26(12): 1103-6. [PubMed] [Google Scholar]
32. Sandholm L. Proteases and their inhibitors in chronic inflammatory periodontal disease. J Clin Periodontol 1986; 13(1): 19-26. [PubMed] [Google Scholar]
33. Windle HJ, Kelleher D. Identification and characterization of a metalloprotease activity from Helicobacter pylori. Infect Immun 1997; 65(8): 3132-7. [PubMed] [Google Scholar]
Send email to the article author
| Rights and Permissions | |
 |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
