[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
:: Volume 23, Issue 6 (Iranian South Medical Journal 2020) ::
Iran South Med J 2020, 23(6): 515-527 Back to browse issues page
Frequency of Resistance Phenotypes and Genotype of Acinetobacter Baumannii and Molecular Typing by PFGE in Patients Admit-ted to the Intensive Care Units of Zanjan Hospi-tals
Maryam Seyyedi1 , Reza Shapouri 2, Habib Zeighami3, Laili Shokoohizadeh4
1- Department of Microbiology, Zanjan Branch, Islamic Azad University, Zanjan, Iran
2- Department of Microbiology, Zanjan Branch, Islamic Azad University, Zanjan, Iran , rezashapoury@yahoo.com
3- Department of Microbiology, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran
4- Department of Medical Microbiology, School of Medicine, Hamadan University of medical sciences, Hamadan, Iran
Abstract:   (487 Views)
Background: By acquiring resistance and through new mechanisms, Acinetobacter baumannii has turned into a global problem in nosocomial infections. The present study was conducted to determine the frequency of resistant strains and resistance genes and also molecular typing of hospital strains in the city of Zanjan. 
Materials and Methods: A total of 250 clinical samples isolated from hospitalized patients in ICUs of Zanjan hospitals were cultured, and identified by the biochemical and blaOXA-51 gene tracking methods. The antibiotic susceptibility test was also run. The presence of integrons 1, 2 and 3, as well as oxacillinase genes was verified by PCR technique, and typing was carried out by the PFGE. The relationship of resistance genes with the antibiotic susceptibility pattern and clusters was determined.
Results: Of the 250 samples, 68 isolates were identified as Acinetobacter, of which, 48 were Acinetobacter baumannii. 94% of the samples were identified as MDR strains, 46% as XDR strains, and 12% as PDR. The highest resistance of the 48 isolates of A. baumannii was attributed to ceftazidime, cefotaxime, and cotrimoxazole, and the highest susceptibility to Colistin (60%). The frequencies of resistance genes of integrons 1, 2, and 3 were 60%, 28%, and 0% respectively. The frequencies of carbapenem resistance genes for blaOXA-23, blaOXA-24, blaOXA-51, and blaOXA-58 were 74%, 24%, 100%, and 4%, respectively. The above samples were grouped into 26 palsutypes.
Conclusion: In the study setting, resistance to Colistin showed an ascending trend, and high genetic diversity suggested diversity of A. baumannii strains.
Keywords: Acinetobacter Baumannii, Integron, Oxacillinase, PFGE, Molecular typing, Antibiotic resistance
Full-Text [PDF 605 kb]   (144 Downloads)    
Type of Study: Original | Subject: Microbiology and Immunology
Received: 2020/06/1 | Accepted: 2020/09/16 | Published: 2020/12/21
References
1. Anvarinejad M, Japoni A, Davarpanah MA, et al. Phenotypic And Molecular Epidemiology Acinetobacter Calcoaceticus Baumannii Complex Strains Spread At Nemazee Hospital Of Shiraz, Iran. Jundishapur J Microbiol 2015; 8(6): e19180. [DOI:10.5812/jjm.8(5)2015.19180]
2. Gholipur A, Ansari N, Damavandi MS, et al. Genotyping And Antibiotic Resistance Of Acinetobacter Baumannii Strains Isolated From Patients Hospitalized In Teaching Hospitals Of Shahrekord By Pulsed- Field Gel Electrophorsis. J Shahid Sadoughi Univ Med Sci 2017; 25(3): 241-51. (Persian)
3. Shirmohammadlou N, Zeighami H, Haghi F, et al. Resistance Pattern And Distribution Of Carbapenemase And Antiseptic Resistance Genes Among Multidrug-Resistant Acinetobacter Baumannii Isolated From Intensive Care Unit Patients. J Med Microbiol 2018; 67(10): 1467-73. [DOI:10.1099/jmm.0.000826]
4. Ardeshiri N, Nasrollahi M, Goudarzi H, et al. The Prevalence Of Integron 1, 2 And 3 Classes In Acinetobacter Baumanii Clinical Isolates From Sari Hospitals, Iran. Res Med 2017; 41(3): 217-25. (Persian)
5. Golestani Eimani B, Ansarin K, Sahebi L, et al. Molecular Typing of Mycobacterium Tuberculosis Isolated from Iranian Patients Using Highly Abundant Polymorphic GC- Rich-Repetitive Sequence. Iran South Med J 2020; 23(2): 87-98. (Persian)
6. Saeedi S, Abdolsalehi MR, Khodabandeh M, et al. Survey Of Integron Types And Carbapenem Resistance Encoding Genes In Acinetobacter Baumannii Isolated From Burn Wound Samples. Alborz Univ Med J 2018; 7(4): 323-32. (Persian) [DOI:10.29252/aums.7.4.323]
7. Keikha M, Jadidi H. Meta-Analysis Study For Antibiotic Resistance Of Acinetobacter Baumannii Clinical Isolates. Iran J Med Microbiol 2017; 11(2): 69-74. (Persian)
8. Simo Tchuinte PL, Rabenandrasana MAN, Kowalewicz C, et al. Phenotypic And Molecular Characterisations Of Carbapenem-Resistant Acinetobacter Baumannii Strains Isolated In Madagascar. Antimicrob Resist Infect Control 2019; 8: 31. [DOI:10.1186/s13756-019-0491-9]
9. Golafshani FB, Kaboosi H, Armaki MT, et al. Molecular Investigation Of Integron Types And Imipenem-Resistance Encoded Genes In Acinetobacter Baumannii Strains Isolated From Burns Patients In Iran. Gene Rep 2019; 17: 100486. [DOI:10.1016/j.genrep.2019.100486]
10. Mirnejad R, Mostofi S, Masjedian F. Role Of Class 2 Integron In Antibiotic Susceptibility Pattern Of Acinetobacter Baumannii Strains Isolated From Hospitals In Tehran. Avicenna J Clin Med 2012; 18(4): 22-8. (Persian)
11. Nourbakhsh F, Nourbakhsh V, Jafakesh MT. Prevalence Of Class I, II And III Integrons In The Antibiotic-Resistant Isolates Of A. Baumannii Detected From Patients Hospitalized In Medical Centers Of Shahrekord. Feyz 2016; 20(5): 461-8. (Persian)
12. Vakili B, Fazeli H, Shoaei P, et al. Detection Of Colistin Sensitivity In Clinical Isolates Of Acinetobacter Baumannii In Iran. J Res Med Sci 2014; 19(Suppl 1): S67-S70.
13. Bahador A, Taheri M, Pourakbari B, et al. Emergence Of Rifampicin, Tigecycline, And Colistin-Resistant Acinetobacter Baumannii In Iran; Spreading Of MDR Strains Of Novel International Clone Variants. Microb Drug Resist 2013; 19(5): 397-406. [DOI:10.1089/mdr.2012.0233]
14. Herrera ME, Mobilia LN, Posse GR. Comparative Evaluation Of The Sensitivity Of Acinetobacter To Colistin, Using The Prediffusion And Minimum Inhibitory Concentration Methods: Detection Of Heteroresistant Isolates. Rev Argent Microbiol 2011; 43(2): 115-9.
15. Agodi A, Voulgari E, Barchitta M, et al. Spread Of A Carbapenem- And ColistinResistant Acinetobacter Baumannii ST2 Clonal Strain Causing Outbreaks In Two Sicilian Hospitals. J Hosp Infect 2014; 86(4): 260-6. [DOI:10.1016/j.jhin.2014.02.001]
16. Leite GC, Oliveira MS, Perdigao-Neto LV, et al. Antimicrobial Combinations Against Pan-Resistant Acinetobacter Baumannii Isolates With Different Resistance Mechanisms. PloS One 2016; 11(3): e0151270. [DOI:10.1371/journal.pone.0151270]
17. Mengeloğlu FZ, Copur Çiçek A, Koçoğlu E, et al. Carriage Of Class 1 And 2 Integrons In Acinetobacter Baumannii And Pseudomonas Aeruginosa Isolated From Clinical Specimens And A Novel Gene Cassette Array: blaOXA-11-cmlA7. Mikrobiyol Bul 2014; 48(1): 48-58.
18. Peymani A, Farajnia S, Nahaei MR, et al. Prevalence Of Class 1 Integron Among Multidrug-Resistant Acinetobacter Baumannii In Tabriz, Northwest Of Iran. Pol J Microbiol 2012; 61(1): 57-60. [DOI:10.33073/pjm-2012-007]
19. Japoni-Nejad A, Farshad S, Van Belkum A, et al. Novel Cassette Array In A Class 1 Integron In Clinical Isolates Of Acinetobacter Baumannii From Central Iran. Int J Med Microbiol 2013; 303(8): 645-50. [DOI:10.1016/j.ijmm.2013.09.005]
20. Mirnejad R, Mostofi S, Masjedian F. Antibiotic Resistance And Carriage Class 1 And 2 Integrons In Clinical Isolates Of Acinetobacter Baumannii From Tehran, Iran. Asian Pac J Trop Biomed 2013; 3(2): 140-5. [DOI:10.1016/S2221-1691(13)60038-6]
21. Lin MF, Liou ML, Tu CC, et al. Molecular Epidemiology Of Integron-Associated Antimicrobial Gene Cassettes In The Clinical Isolates Of Acinetobacter Baumannii From Northern Taiwan. Ann Lab Med 2013; 33(4): 242-7. [DOI:10.3343/alm.2013.33.4.242]
22. Raka L, Kalenć S, Bosnjak Z, et al. Molecular Epidemiology Of Acinetobacter Baumannii In Central Intensive Care Unit In Kosova Teaching Hospital. Braz J Infect Dis 2009; 13(6): 408-13. [DOI:10.1590/S1413-86702009000600004]
23. Ranjbar R, Farahani A. Study Of Genetic Diversity, Biofilm Formation, And Detection Of Carbapenemase, MBL, ESBL, And Tetracycline Resistance Genes In Multidrug-Resistant Acinetobacter Baumannii Isolated From Burn Wound Infections In Iran. Antimicrob Resist Infect Control 2019; 8: 172. [DOI:10.1186/s13756-019-0612-5]
24. Eghbalimoghadam M, Farahani A, Akbar FN, et al. Frequency Of Class 1 Integron And Genetic Diversity Of Acinetobacter Baumannii Isolated From Medical Centers In Kermanshah. J Nat Sci Biol Med 2017; 8(2): 193-8. [DOI:10.4103/0976-9668.210007]
Send email to the article author

Add your comments about this article
Your username or Email:

CAPTCHA


XML   Persian Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Seyyedi M, Shapouri R, Zeighami H, Shokoohizadeh L. Frequency of Resistance Phenotypes and Genotype of Acinetobacter Baumannii and Molecular Typing by PFGE in Patients Admit-ted to the Intensive Care Units of Zanjan Hospi-tals. Iran South Med J. 2020; 23 (6) :515-527
URL: http://ismj.bpums.ac.ir/article-1-1372-en.html


Volume 23, Issue 6 (Iranian South Medical Journal 2020) Back to browse issues page
دانشگاه علوم پزشکی بوشهر، طب جنوب ISMJ

Iranian South Medical Journal is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License which allows users to read,
copy, distribute and make derivative works for non-commercial purposes from the material, as long as the author of the original work is cited properly

Copyright © 2017, Iranian South Medical Journal| All Rights Reserved

Persian site map - English site map - Created in 0.05 seconds with 29 queries by YEKTAWEB 4296