:: Volume 6, Issue 2 (Iranian south medical of journals 2004) ::
Iran South Med J 2004, 6(2): 97-103 Back to browse issues page
Isolation and purification of a novel anticancer 60 K daltons protein from the Persian Gulf sea hare, Aplysia Dactylomela
Keyvan Zandi *1, Mohammad Hojat Farsangi, Iraj Nabipour, Masood Soleimani, Khosro Khajeh, Reza Sajedi, Seyed Mojtaba Jafary, Abbas Ghadery
1- , zandi@dr.com
Abstract:   (16447 Views)
Sea hares have attracted the interest of many workers investigating chemical defense substances. Most of these substances are low molecular weight compounds derived from algal diets. Anticancer effects of a novel protein isolated from purple fluid of A. dactylomela are reported. The purification procedure consisted basically of ammonium sulphate fractionation, ion exchange and ultrafiltration techniques. For cytotoxicity effects, L929, K562, HL60 and NB4 cell lines and MTT assay were used. A protein of 60000 Da of the purple fluid of A. dactylomela had antiproliferative effects on the cell lines it was maximally active at 0.5-1.5 microgram/ml on NB4 cell line. Therefore, the purple fluid of A. dactylomela has a novel antiproliferative agent.
Keywords: Sea hare, anticancer, cancer, neoplastic
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Type of Study: Original | Subject: General
Received: 2009/01/21 | Accepted: 2009/01/21 | Published: 2009/01/21


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