:: Volume 23, Issue 1 (Iranian South Medical Journal 2020) ::
Iran South Med J 2020, 23(1): 1-13 Back to browse issues page
Detection of Heavy Metals Resistance Genes and Effects of Iron Nanoparticles on the Gene Expression in Pseudomonas Aeruginosa Using Real-Time PCR
Marzeah Rostami1 , Kumarss Amini 2, Babak Kheirkhah3
1- Department of Microbiology, School of Basic Sciences, Sirjan Branch, Islamic Azad University, Sirjan, Iran, Iran
2- Department of Microbiology, School of Basic Sciences, Saveh Branch, Islamic Azad University, Saveh, Iran , dr_kumarss_amini@yahoo.com
3- Department of Microbiology, school of Basic Sciences, Kerman Branch, Islamic Azad University, Kerman, Iran
Abstract:   (1250 Views)
Background: Heavy metals enter the environment through industrial activities and contaminate natural ecosystems. Identification of heavy metal-resistant bacteria plays an important role in environmental
pollution and ultimately cleansing it. Therefore, the aim of the present study was to isolate the resistant genes of Pseudomonas aeruginosa and the effects of nanoparticles on gene expression using real-time PCR.
Materials and Methods In this descriptive cross-sectional study, 60 isolates of P. aeruginosa were studied. Frequency of czr gene was determined by PCR. Also, the effects of iron nanoparticles on czr gene expression were evaluated by real-time PCR after RNA extraction.
Results: In this study, 25 isolates were carriers of czr gene. Also, iron nanoparticles could reduce the
expression of the heavy metal resistance gene in P. aeruginosa in vitro.
Conclusion: This study showed that the resistance of different species of P. aeruginosa to the heavy metal cadmium is different.
Keywords: Pseudomonas aeruginosa, Heavy metals, czr gene, Real-time PCR.
Full-Text [PDF 736 kb]   (287 Downloads)    
Type of Study: Original | Subject: Microbiology and Immunology
Received: 2019/01/12 | Accepted: 2019/11/3 | Published: 2020/03/28
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