[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
:: Volume 25, Issue 3 (Iranian South Medical Journal 2022) ::
Iran South Med J 2022, 25(3): 198-209 Back to browse issues page
The Anticancer Effects of Sargassum Boveanum Hydroalcoholic Extract in Human Colorectal Cancer Cell Lines
Ali Reza Khosravani1 , Samad Akbarzadeh2, Ali Movahed2, Hajar Jaberie * 3
1- Student Research Committee, Bushehr University of Medical Sciences, Bushehr, Iran
2- Department of Biochemistry, School of Medicine, Bushehr University of Medical Sciences, Bushehr, Iran
3- Department of Biochemistry, School of Medicine, Bushehr University of Medical Sciences, Bushehr, Iran , jaberiehajar@yahoo.com
Abstract:   (333 Views)
Background: Colorectal cancer (CRC) is one of the leading causes of death worldwide. Sargassum boveanum is a plant species of brown algae that is widely found in the Persian Gulf. The present study investigated the antioxidant and cytotoxic activity of the hydroalcoholic extract of Sargassum boveanum in colorectal cancer cell lines.
Materials and Methods: Sargassum boveanum was collected from the Persian Gulf in Bushehr province. The hydroalcoholic extract of this plant was extracted by maceration in ethanol 70%. The concentrations of polyphenols, flavonoids and antioxidant activity of the hydroalcoholic extract (ethanol 70%) were assessed by folin ciocalteu, aluminum chloride and CUPRAC methods, respectively. Afterwards, the cytotoxicity effect of this extract was investigated in two colorectal cancer cell lines (SW742 and HCT116) and normal cells (fibroblasts) at 24, 48 and 72 hours post-treatment by the MTT method.
Results: The studied hydroalcoholic extract contains significant amounts of polyphenol (36.79 ± 0.66 mg GAE/g dwt), flavonoid (194.66±2.5 QE/g dwt) and antioxidant capacity (526.99 ± 9.16 mg AA/g dwt). The hydroalcoholic extract gradually inhibited cell growth in the SW742, HCT116 and fibroblast cells in a
time- and dose-dependent manner.

Conclusion: Our results showed that sargassum boveanum hydroalcoholic extract may have considerable potential for development as a novel, natural product-based, anticancer agent.
Keywords: Sargassum Boveanum, Colorectal Cancer, Hydroalcoholic Extract, Antioxidant, Cytotoxicity
Full-Text [PDF 545 kb]   (88 Downloads)    
Type of Study: Original | Subject: Biochemistry. Cell Biology and Genetics
Received: 2022/03/6 | Accepted: 2022/07/23 | Published: 2022/09/22
1. Guo M, Jin N, Pawlik T, et al. Neoadjuvant chemotherapy for colorectal liver metastases: A contemporary review of the literature. World J Gastrointest Oncol 2021; 13(9): 1043-61. [DOI]
2. Rebecca L, Siegel RL, Kimberly D, et al. Cancer statistics, 2020. CA Cancer J Clin 2020; 70(1): 7-30. [DOI]
3. Arnold M, Sierra MS, Laversanne M, et al. Global patterns and trends in colorectal cancer incidence and mortality. Gut 2017; 66(4): 683-91. [DOI]
4. Iran, Islamic Republic of. 364-iran-islamicrepublic-of-fact-sheets.pdf. (Accessed September 11, 2022, at https://gco.iarc.fr/today/data/factsheets/populations/364-iran-islamic-republic-of-fact-sheets.pdf) [Article]
5. Senapati S, Mahanta AK, Kumar S, et al. Controlled drug delivery vehicles for cancer treatment and their performance. Signal Transduct Target Ther 2018; 3: 7. [DOI]
6. Rayan A, Raiyn J, Falah M. Nature is the best source of anticancer drugs: Indexing natural products for their anticancer bioactivity. PLoS one 2017; 12(11): e0187925-e. [DOI]
7. Schirrmacher V. From chemotherapy to biological therapy: A review of novel concepts to reduce the side effects of systemic cancer treatment (Review). Int J Oncol 2019; 54(2): 407-19. [DOI]
8. Rejhová A, Opattová A, Čumová A, et al. Natural compounds and combination therapy in colorectal cancer treatment. Eur J Med Chem 2018; 144: 582-94. [DOI]
9. Leandro A, Pereira L, Gonçalves AMM. Diverse Applications of Marine Macroalgae. Mar Drugs 2020; 18(1): 17. [DOI]
10. Wada S, Ishida K-I, Noda M, et al. Marine Algae and Plants. In: Inaba K, Hall-Spencer JM, editors. Japanese Marine Life: A Practical Training Guide in Marine Biology. Singapore: Springer Singapore, 2020, 49-64.
11. Generalić Mekinić I, Skroza D, Šimat V, et al. Phenolic Content of Brown Algae (Pheophyceae) Species: Extraction, Identification, and Quantification. Biomolecules 2019; 9(6): 244. [DOI]
12. Kumar Y, Tarafdar A, Kumar D, et al. Evaluation of Chemical, Functional, Spectral, and Thermal Characteristics of Sargassum wightii and Ulva rigida from Indian Coast. Journal of Food Quality 2021; 2021: 9. [DOI]
13. Patra JK, Rath SK, Jena K, et al. Evaluation of antioxidant and antimicrobial activity of seaweed (Sargassum sp.) extract: A study on inhibition of Glutathione-S-Transferase activity. Turk J Biol 2008; 32(2): 119-25. [Article]
14. Kim YH, Kim EH, Lee C, et al. Two new monogalactosyl diacylglycerols from brown alga Sargassum thunbergii. Lipids 2007; 42(4): 395-9. [DOI]
15. Yegdaneh A, Ghannadi A, Dayani L. Chemical constituents and biological activities of two Iranian Cystoseira species. Res Pharm Sci 2016; 11(4): 311-7. [DOI]
16. Ermakova S, Sokolova R, Kim SM, et al. Fucoidans from brown seaweeds Sargassum hornery, Eclonia cava, Costaria costata: structural characteristics and anticancer activity. Appl Biochem Biotechnol 2011; 164(6): 841-50. [DOI]
17. Na HJ, Moon PD, Ko SG, et al. Sargassum hemiphyllum inhibits atopic allergic reaction via the regulation of inflammatory mediators. J Pharmacol Sci 2005; 97(2): 219-26. [DOI]
18. Kang JY, Khan M, Park N, et al. Antipyretic, analgesic, and anti-inflammatory activities of the seaweed Sargassum fulvellum and Sargassum thunbergii in mice. J Ethnopharmacol 2008; 116(1): 187-90. [DOI]
19. Lee HJ, Kim YA, Ahn J-W, et al. Screening of Korean marine plants for their inhibitory effect on histamine release from RPMC in vitro. Biotechnol Bioprocess Eng 2006; 11(1): 80-83. [DOI]
20. Gany SA, Tan SC, Gan SY. Antioxidative, anticholinesterase and anti-neuroinflammatory properties of Malaysian brown and green seaweeds. World Acad Sci Eng Technol 2015; 8: 1269-75. [DOI]
21. Afonso C, Correia AP, Freitas MV, et al. Seasonal Changes in the Nutritional Composition of Agarophyton vermiculophyllum (Rhodophyta, Gracilariales) from the Center of Portugal. Foods 2021; 10(5): 1145. [DOI]
22. Aroyehun AQ, Palaniveloo K, Ghazali F, et al. Effects of Seasonal Variability on the Physicochemical, Biochemical, and Nutritional Composition of Western Peninsular Malaysia Gracilaria manilaensis. Molecules 2019; 24(18): 3298. [DOI]
23. Sohrabipour J, Rabei R, Nejadsatari T, et al. The marine algae of the Southern coast of Iran, Persian Gulf, Lengeh area. Iran Journ Bot 2004; 10(2): 83-93. [DOI]
24. Liu RH. Potential synergy of phytochemicals in cancer prevention: mechanism of action. J Nutr 2004; 134(12 Suppl): 3479S-3485S. [DOI]
25. Tatar M, Bagheri Z, Varedi M, et al. Blackberry Extract Inhibits Telomerase Activity in Human Colorectal Cancer Cells. Nutr Cancer 2019; 71(3): 461-71. [DOI]
26. Hudz N, Yezerska O, Shanaida M, et al. Application of the Folin-Ciocalteu method to the evaluation of Salvia sclarea extracts. Pharmacia 2019; 66(4): 209-15. [DOI]
27. Ahmed F, Iqbal M. Antioxidant activity of Ricinus Communis. Organic & Medicinal Chem IJ 2018; 5(4): 107-12. [DOI]
28. Munteanu IG, Apetrei C. Analytical Methods Used in Determining Antioxidant Activity: A Review. Int J Mol Sci 2021; 22(7): 3380. [DOI]
29. Yu W, Qin X, Jin Y, et al. Tianshengyuan-1 (TSY-1) regulates cellular Telomerase activity by methylation of TERT promoter. Oncotarget 2017; 8(5): 7977-88. [DOI]
30. Buranaamnuay K. The MTT assay application to measure the viability of spermatozoa: A variety of the assay protocols. Open Vet J 2021; 11(2): 251-69. [DOI]
31. Rashidi M, Seghatoleslam A, Namavari M, et al. Selective Cytotoxicity and ApoptosisInduction of Cyrtopodion scabrum Extract Against Digestive Cancer Cell Lines. Int J Cancer Manag 2017; 10(5): e8633. [DOI]
32. Wali AF, Majid S, Rasool S, et al. Natural products against cancer: Review on phytochemicals from marine sources inpreventing cancer. Saudi Pharm J 2019; 27(6): 767-77. [DOI]
33. Ward GM, Faisan Jr JP, Cottier-Cook EJ, et al. A review of reported seaweed diseases and pests in aquaculture in Asia. J World Aquacult Soc 2020; 51(4): 815-28. [DOI]
34. Wells ML, Potin P, Craigie JS, et al. Algae as nutritional and functional food sources: revisiting our understanding. J Appl Phycol 2017; 29(2): 949-82. [DOI]
35. Khalifeh T, Vazirizadeh A, Mohebbi GH, et al. Determination of some Nutraceutical Compounds, Amino Acids and Fatty acids Present in the Extracts of Sargasum boveanum Algae Obtained from the Coastal Waters of Central Bushehr, Iran. Iran South Med J 2021; 24(2): 134-59. [Article]
36. Cotas J, Pacheco D, Gonçalves AM, et al. Seaweeds’ nutraceutical and biomedical potential in cancer therapy: a concise review. J Cancer Metastasis Treat 2021; 7: 13. [DOI]
37. Zhang J, Guo H, Zhang H, et al. Putative tumor suppressor miR-145 inhibits colon cancer cell growth by targeting oncogene Friend leukemia virus integration 1 gene. Cancer 2011; 117(1): 86-95. [DOI]
38. Leibovitz A, Stinson JC, McCombs WB, et al. Classification of human colorectal adenocarcinoma cell lines. Cancer Res 1976; 36(12): 4562-9. [DOI] [PubMed]
39. Usui G, Masuda Y, Hashimoto H, et al. Colon Metastasis From Microscopic Serous Carcinoma of the Fallopian Tube Fimbria Mimicking a Primary Colon Cancer. Int J Surg Pathol 2019; 27(4): 390-5. [DOI]
40. Wiji Prasetyaningrum P, Bahtiar A, Hayun H. Synthesis and Cytotoxicity Evaluation of Novel Asymmetrical Mono-Carbonyl Analogs of Curcumin (AMACs) against Vero, HeLa, and MCF7 Cell Lines. Sci Pharm 2018; 86(2): 25. [DOI]
41. Aggarwal V, Tuli HS, Varol A, et al. Role of Reactive Oxygen Species in Cancer Progression: Molecular Mechanisms and Recent Advancements. Biomolecules 2019; 9(11): 735. [DOI]
42. Afrin S, Forbes-Hernandez TY, Gasparrini M, et al. Strawberry-Tree Honey Induces Growth Inhibition of Human Colon Cancer Cells and Increases ROS Generation: A Comparison with Manuka Honey. Int J Mol Sci 2017; 18(3): 613. [DOI]
43. Gasparrini M, Afrin S, Forbes-Hernández TY, et al. Protective effects of Manuka honey on LPS-treated RAW 264.7 macrophages. Part 2: Control of oxidative stress induced damage, increase of antioxidant enzyme activities and attenuation of inflammation. Food Chem Toxicol 2018; 120: 578-87. [DOI]
44. Gunasekaran S, Venkatachalam K, Namasivayam N. p-Methoxycinnamic acid, an active phenylpropanoid induces mitochondrial mediated apoptosis in HCT-116 human colon adenocarcinoma cell line. Environ Toxicol Pharmacol 2015; 40(3): 966-74. [DOI]
45. Raja SB, Rajendiran V, Kasinathan NK, et al. Differential cytotoxic activity of Quercetin on colonic cancer cells depends on ROS generation through COX-2 expression. Food Chem Toxicol 2017; 106(Pt A): 92-106. [DOI]
46. Mehdinezhad N, Ghannadi A, Yegdaneh A. Phytochemical and biological evaluation of some Sargassum species from Persian Gulf. Res Pharm Sci 2016; 11(3): 243-9. [Article]
47. Vaseghi G, Sharifi M, Dana N, et al. Cytotoxicity of Sargassum angustifolium partitions against breast and cervical cancer cell lines. Adv Biomed Res 2018; 7: 43. [DOI]
Send email to the article author

Add your comments about this article
Your username or Email:


XML   Persian Abstract   Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

khosravani A R, Akbarzadeh S, Movahed A, Jaberie H. The Anticancer Effects of Sargassum Boveanum Hydroalcoholic Extract in Human Colorectal Cancer Cell Lines. Iran South Med J 2022; 25 (3) :198-209
URL: http://ismj.bpums.ac.ir/article-1-1624-en.html

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 25, Issue 3 (Iranian South Medical Journal 2022) Back to browse issues page
دانشگاه علوم پزشکی بوشهر، طب جنوب ISMJ

Iranian South Medical Journal is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License which allows users to read,
copy, distribute and make derivative works for non-commercial purposes from the material, as long as the author of the original work is cited properly

Copyright © 2022, Iranian South Medical Journal| All Rights Reserved

Persian site map - English site map - Created in 0.06 seconds with 30 queries by YEKTAWEB 4540