:: Volume 14, Issue 2 (Iranian South Medical of Journal 2011) ::
Iran South Med J 2011, 14(2): 94-99 Back to browse issues page
Determination of antimicrobial resistance pattern and Extended-Spectrum Beta Lactamases producing Pseudomonas aeruginosa strains isolated from clinical specimens of Hajar and Kashani Hospitals,Shahrekord 1387
Mana Shojapour1, Majid Validi2, Laleh Shariaty3, Ali Karimi2, Behnam Zamanzad4
1- Arak University of Medical Sciences
2- Shahre-Kord University of Medical Sciences
3- Tehran University of Medical Sciences
4- Shahre-Kord University of Medical Sciences , bzamanzad@yahoo.com
Abstract:   (19751 Views)

Background: Pseudomonas aeruginosa is one of the leading causes of hospital infections in patients hospitalized for a 10 day period or over. It is also considered to be the most important cause of the burn wound infection. Approximately 75% of deaths in burned patients are due to wound infection and the subsequent septicemia. Clinical use of antibiotics has increasingly led to the global distribution of P. aeruginosa isolates with multi-drug resistance. The study was launched to determine the antimicrobial susceptibility pattern and the presence of the extended-spectrum-beta lactamase (ESBL) in P.aeruginosa strains isolated from clinical specimens. Methods: Totally, 175 P. aeruginosa strains were isolated from clinical samples and identified by standard methods. The pattern of antimicrobial resistance was then performed on the isolates using Disk Agar Diffusion (DAD) according to CLSI Guideline. Primary screening test for ESBL producing strains was performed by ceftazidim antibiotic disk using disk diffusion method. Combined disk method was used to confirm ESBL producing bacteria. Results: The rate of antimicrobial resistance of P.aeruginosa isolates were 64% to ticarcillin, 52.2% to cefepime, 68.6% to ticarcillin/clavolanic acid, 68.6% to ceftazidime, 67.4% to amikacin, 68.6% to gentamicin, 48% to imipenem, 77.7% to ciprofloxacin and 5.1% to polymixcine B. In the primary screening test, 120 isolates of P.aeruginosa strains were resistant to ceftazidime. In the combined disk method, 66 isolates (55%) were positive for ESBLs. Conclusion: Polymixcine B was found to be the most effective antimicrobial agent in this study. Bacteria carrying ESBL genes may increase mortality and morbidity. Thus, their accurate diagnosis is of extreme importance to prevent from the treatment failure resulted from improper antibiotic administration.

Keywords: antibiotic resistance, combined disk method, ESBLs, pseudomonas aeruginosa
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Type of Study: Original | Subject: General
Received: 2010/03/2 | Accepted: 2010/07/17 | Published: 2011/03/15


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Volume 14, Issue 2 (Iranian South Medical of Journal 2011) Back to browse issues page