Background: Ciprofloxacin is a synthetic antibacterial agent belonging to the family offluoroquinoloneswith a very broad spectrum against of microbial pathogens, especially Gram-negative infectious diseases, that has been approved in more world-widecountries. The aim of this study was planned to seeeffects of ciprofloxacin after inducement, in rat Spermatogenesis. Material and Methods: The twenty male wistar rat were selected and randomly divided into two groups control (n=10) and test (n=10). The test group was received 12.5mg/kg (PO) ciprofloxacin daily for sixty day however the control group just received plate. In sixtieth day the testis tissue of Rat in both groups were remove and prepared for histomorphomotric study and 5cc blood samples were taken for Testosterone analysis. Results: Light microscopic observation, Score count study in seminiferous tubules confirmed most of tubules were in Score2, Score4-5, Score 6-9, and these scoreswere significantly in P<(0.05), and this results confirmed tubular, Sertoli cells syndrome, hypospermatogenisis in experimental group ,in other hand most seminiferous tubules in control group was significantly in Score 10 P<(0.05), and this dataconfirmed intake tubules. There was a marked decrease in serum testosterone hormone, in experimental group as compared with control group, the statically analysis was fishers method (P<0.05). Conclusion: Since in our study ciprofloxacin had decreasing side effect on Spermatogenesis developmentin rat, it was suggested that using ciprofloxacin maybe cause harmful effects on seminiferous tubular.