Volume 20, Issue 3 (Iranian South Medical Journal 2017)                   Iran South Med J 2017, 20(3): 267-277 | Back to browse issues page

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Identification of E. coli O157:H7 by Using Specific Primers for rfbE and stx2b Genes
Mostafa Bakhshi1 , Firouz Ebrahimi1 , Shahram Nazarian * 2, Yousef Tarverdizade1 , Davood Sadeghi1
1- Department of Biology, School of Basic Science, Imam Hossein University, Tehran, Iran
2- Department of Biology, School of Basic Science, Imam Hossein University, Tehran, Iran , kpnazari@ihu.ac.ir
Abstract:   (8829 Views)

Background: E. coli O157:H7 is one of the intestinal pathogens which can cause severe lesions in the gastrointestinal system. These bacteria can produce toxins and are the main cause of hospital infections. Their detection is usually done by culture on sorbitol-MacConkey agar which is a time-consuming test. The aim of this study was to develop a rapid, yet accurate method to identify this bacterium using a PCR based technique.
Material and Methods: rfbE and stx2b genes were selected for identification of specific E. coli O157:H7 strain. Then amplification was performed by PCR following designing specific primers. Sorbitol-MacConkey agar was used to verification of growth ability of selected colonies during PCR.
Results: By the appearance of the bonds belong to rfbE and stx2B genes on an agarose gel, the ability of designed primers for gene detection in samples of E .coli O157:H7 was verified. A sorbitol-MacConkey agar medium was used to evaluate the growing potency of colonies selected during PCR.
Conclusion: In this study we demonstrated that we could develop a fast, accurate, and relatively comfortable method for detection of E. coli O157:H7 strain by using PCR technique and specific primers instead of using current methods.
 
 

Keywords: Identification of E. coli O157:H7 strain, polymerase chain reaction, rfbE gene, stx2b gene
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Type of Study: Original | Subject: Biochemistry. Cell Biology and Genetics
Received: 2016/11/7 | Accepted: 2017/01/9 | Published: 2017/07/5
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