RT - Journal Article T1 - Isolation and purification of a novel anticancer 60 K daltons protein from the Persian Gulf sea hare, Aplysia Dactylomela JF - ISMJ YR - 2004 JO - ISMJ VO - 6 IS - 2 UR - http://ismj.bpums.ac.ir/article-1-113-en.html SP - 97 EP - 103 K1 - Sea hare K1 - anticancer K1 - cancer K1 - neoplastic AB - Sea hares have attracted the interest of many workers investigating chemical defense substances. Most of these substances are low molecular weight compounds derived from algal diets. Anticancer effects of a novel protein isolated from purple fluid of A. dactylomela are reported. The purification procedure consisted basically of ammonium sulphate fractionation, ion exchange and ultrafiltration techniques. For cytotoxicity effects, L929, K562, HL60 and NB4 cell lines and MTT assay were used. A protein of 60000 Da of the purple fluid of A. dactylomela had antiproliferative effects on the cell lines it was maximally active at 0.5-1.5 microgram/ml on NB4 cell line. Therefore, the purple fluid of A. dactylomela has a novel antiproliferative agent. LA eng UL http://ismj.bpums.ac.ir/article-1-113-en.html M3 ER -