TY - JOUR T1 - Rapid detection of Staphylococcus aureus and genus Staphylococcus by Fluorescent In Situ Hybridization (FISH) TT - تشخیص سریع استافیلوکوکوس اورئوس و جنس استافیلوکوک با روش هیبریدیزاسیون در موضع توسط پروب های فلوئورسنت (FISH) JF - ISMJ JO - ISMJ VL - 6 IS - 2 UR - http://ismj.bpums.ac.ir/article-1-114-en.html Y1 - 2004 SP - 104 EP - 114 KW - Staphylococcus KW - In Situ Hybridization KW - Fluorescent KW - Probe KW - 16S rRNA KW - FISH N2 - Fluorescent in situ hybridization ( FISH ) is a new diagnostic technique for specific detection of microorganisms at molecular level and direct visualization of microbial morphology. In this study, FISH technique was evaluated for detection of staphylococci. A set of 16S rRNA-targeted oligonucleotide probes were used for FISH. For detection of Staphylococcus aureus in sputum samples by FISH and comparison with conventional bacterial culture, 76 sputum samples were investigated . According to our data , sensitivity and specificity of FISH for detection of this bacterium were 86.7% and 100% , respectively. As another part of this study, 41 blood culture specimens were examined for detection of staphylococci. In compare with conventional culture methods, sensitivity and specificity of FISH for detection of S. aureus were 100% and for non S. aureus species of staphylococci, sensitivity and specificity of FISH were 95.7% and 100% respectively. All steps of FISH technique were performed within 3h. Therefore, FISH is a rapid, sensitive, and specific technique for detection of staphylococci. M3 ER -