Bushehr University of Medical Sciences
Iranian South Medical Journal
1735-4374
20
5
2017
11
1
Association of c.*49T>C and c.-932 G>A Polymorphisms of CYBA Gene with Coronary Artery Disease: a Case-Control Study in Kashan Population
416
425
FA
Tahereh
Mazoochi
Anatomical Sciences Research Center, Kashan University of Medical Sciences, Kashan, Iran
Mohammad
Karimian
Anatomical Sciences Research Center, Kashan University of Medical Sciences, Kashan, Iran<br>Department of Molecular and Cell Biology, School of Basic Sciences, University of Mazandaran, Babolsar, Iran
Majid
Mazoochi
Department of Cardiology, School of Medicine, Kashan University of Medical Sciences, Kashan, Iran
Abasalt
Hosseinzadeh Colagar
Department of Molecular and Cell Biology, School of Basic Sciences, University of Mazandaran, Babolsar, Iran
Background: The CYBA (Cytochrome B-245 Alpha Chain; p22phox) gene encodes an essential subunit of NADH/NADPH-oxidase. This enzyme is expressed in smooth muscle cells of arteries which produces several active oxygen products. On the other hand, oxidative stress has a significant role in the pathogenesis of coronary artery disease (CAD). This study aimed to investigate the association between rs7195830 (c.*49T>C) and rs9932581 (c.-930G>A) polymorphisms in CYBA gene with coronary artery disease in an Iranian population.
Materials and Methods: In this case-control study, citrated blood samples were obtained from 180 participants, including 85 patient with CAD and 95 healthy volunteers. The fragments containing rs7195830 and rs9932581 of CYBA gene from extracted genome were amplified by polymerase chain reaction. Then the genotype of samples was detected using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. Genetic association analysis was performed using logistic regression test.
Results: Data analysis of c.-930G>A polymorphism revealed a significant association between AA genotype and risk of CAD (OR: 2.71, 95%CI: 1.04-7.06, p= 0.041). Also, the allelic analysis revealed that there was a significant association between A allele and CAD risk (OR: 1.65, 95%CI: 1.05-2.57, p=0.029), while there was no significant association between c.*49T>C polymorphism and risk of CAD.
Conclusion: The study results showed that the c.-930G>A polymorphism might be associated with the susceptibility to CAD and could be considered as a potential biomarker in future studies.
Bushehr University of Medical Sciences
Iranian South Medical Journal
1735-4374
20
5
2017
11
1
Molecular Typing Isolates of Salmonella Enterica Serovar Infantis Using Eric-PCR Method
426
436
FA
Abolfazl
Moghadam
Department of Cell and Molecular Science, School of Biology, University of tehran, Tehran, Iran
Shahram
Nazarian
Department of Biology, School of Science, Imam Hossain University, Tehran, Iran
Background: Salmonella are significant bacteria of the Enterobacteriaceae which are very diverse biochemically and serologically. These bacteria are primarily transmitted through food ingestion. The spread of non-typhoid Salmonella is one of the challenging issues in the current medical research. Therefore, rapid diagnosis and identifying the type of these pathogens provide crucial information for early detection and controlling the spread of the infection. The aim of this study was typing the clinical strains of Salmonella Infantis.
Materials and Methods: In this study, strains of Salmonella Infantis were isolated from several health centers. All of the strains were identified by standard microbiology, biochemical and molecular methods. Genetic relationship between strains was analyzed using the ERIC-PCR method.
Results: In this study, 842 stool and blood sample of patients with diarrhea were examined, and 48 different strains linked to Salmonella Infantis were isolated. Strains categorized into 14 different groups by genotyping using the ERIC-PCR method, and the highest number of the strains were placed in group 5 (20%, 10 strains).
Conclusion: Results of this study revealed that strains of Salmonella Infantis which were examined genetically were rather diverse in nature. This could be due to the high prevalence of polyclonal strains in human samples. It was also shown that ERIC-PCR method has an abundant differential power for the molecular typing purposes.
Bushehr University of Medical Sciences
Iranian South Medical Journal
1735-4374
20
5
2017
11
1
Study of Association Between Polymorphism Alpha7 Neuronal Nicotinic Acetylcholine Receptor Subunit Gene (CHRNA7) with the Development of Schizophrenia in Iranian Population
437
447
FA
Shadi
Haghighat
Department of Genetics, School of Biological Sciences, Tonekabon Branch, Islamic Azad University, Tonekabon, Iran
Rasoul
Zahmatkesh Roodsari
Department of Biology, School of Biological Sciences, Tonekabon Branch, Islamic Azad University, Tonekabon, Iran
Reza
Ranjbar
Molecular Biology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran
Background: SCZ (Schizophrenia) is a common psychiatric and mental in which patients share a common genetic vulnerability. The α7 neuronal nicotinic receptor gene (CHRNA7) is located on the chromosome 15q13-q14. There are several single nucleotide polymorphisms (SNPs) in exons, introns and promoter sites inside the CHRNA7 gene. The aim of this research was to investigate the association between polymorphism CHRNA7 and the development of schizophrenia in Iranian population.
Materials and Methods: This case-control study included 100 patients with schizophrenia and 100 healthy volunteers as a control group. Genomic DNA was extracted from blood samples. Genotypes were determined using the PCR-RFLP method. Statistical analysis was performed using the SPSS software version 20.
Results: Frequency of CC, TCand TT genotypes were 18%, 42% and 40% in patients group and 14%, 45% and 41%, in normal groups respectively. Statistical analysis indicated that in the total sample including male and female, there was no significant association between CHRNA7 gene polymorphism and schizophrenia disease.
Conclusion: The presence of the T allele in CHRNA7 gene at rs904942 cannot be considered as a risk factor for schizophrenia. Therefore, further genetic studies with more SNPs and larger sample sizes with more heterogenicity are required for more conclusive results.
Bushehr University of Medical Sciences
Iranian South Medical Journal
1735-4374
20
5
2017
11
1
Measurement and Evaluation of Ecological Risk of Heavy Metals in Surface Sediments of Pars Special Economic Energy Zone
448
469
FA
Arash
Haghshenas
Institute of Shrimp, Tehran, Iran
Masoud
Hatami-manesh
Department of Environment Sciences, School of Natural Resources and Environment, Malayer University, Malayer, Iran
Mohsen
Mirzaei
Environment Group, Institute of Grapes and Raisins, Malayer University, Malayer, Iran
MirMehrdad
Mir Sanjari
Department of Environment Sciences, School of Natural Resources and Environment, Malayer University, Malayer, Iran
Parisa
Hossein Khezri
Institute of Shrimp, Tehran, Iran
Background: Nowadays, heavy metals pollution in aquatic ecosystems is one of the most significant global environmental issues. Since these metals have characteristics such as chemical stability, low degradability and bioaccumulation ability.Therefore they can affect the food chain at different levels, causing an abundant ecological risks for bioorganisms. This study investigates the concentration of heavy metals (mercury, cadmium, lead, chromium, copper, nickel, zinc and iron) in coastal sediments and assesses its ecological in the Pars Special Economic Energy Zone in the south of Iran.
Materials and Methods: In order to assess the concentration of heavy metals, 16 stations along the coast were selected and three samples were taken from each station. After preparation and digestion of the samples, the concentrations of these metals were determined by using Polarography method.
Results: The results showed that the order of average amount of total concentration of metals in the sediments were (Fe > Zn> Cu > Ni> Cr > Pb > Cd > Hg), reflected as (56.065 ± 2.04 > 45.91±12.035 > 28.58±1.77 > 18.96 ± 0.84 > 8.59±0.47 > 0.447 ± 0.07 > 0.03 ± 0.016) mg/kg respectively. In addition, assessment of indicators of the environmental factors such as; Geo accumulation index (Igeo), Contamination factor (CF), Pollution Load Index (PLI) represents low to modeate pollution in the region. Also, the risk assessment for ecological and environmental effect of heavy metals revealed the order of (Cd >Cu> Pb> Ni> Zn> Cr> Hg) respectively.
Conclusion: According to the results, the pollution in the region affected by heavy metals was low for Hg, Zn, Cr, Pb and Ni.This effect was moderate for Cd and Cu and was high for adjusted cadmium concentrations a by normal amount in the crust of the Earth.High levels of pollution factor along with adjusted pollution for Cd, Cu implies the effects of human activities on pollution and accumulation of these heavy metals in sediments in the region. Based on the calculation of the ecological risk index of metals we can express that among the measured elements, Cadmium sediments are in moderate ecological risk.
Bushehr University of Medical Sciences
Iranian South Medical Journal
1735-4374
20
5
2017
11
1
Water Quality Assessment of Rivers in Bushehr Province by Using Water Quality Index During 2011-2013 Years
470
480
FA
Rohollah
Mirzaei
Department of Environment, School of Natural Resources and Earth Sciences, University of Kashan, Kashan, Iran
Nastaran
Abbasi
Department of Environment, School of Natural Resources and Earth Sciences, University of Kashan, Kashan, Iran
Mohammad
Sakizadeh
Department of Environmental Sciences, School of Sciences, Shahid Rajaee Teacher Training University, Tehran, Iran
Background: Bushehr Province is located in the south west of Iran, and due to water shortage crisis in this province, the water quality assessment of its rivers is very important. This study aimed to assess the quality of water in Bushehr Province rivers including Shapour, Dalaki, Helleh, Baghan, Mond, and Bahoosh revers. We chose these rivers because of their significance for drinking, agricultural and industrial consumption. For the quality assessment, we used NSFWQI and IRWQISC indexes.
Materials and Methods: In this study, water samples were taken from 11 stations along six rivers including Shapour, Dalaki, Helleh, Mond, Bahoosh and Baghan during 2011-2013. The quality of water in
above-mentioned rivers was assessed using NSFWQI and IRWQISC indexes. PO4, NO3, DO, BOD, and COD were the parameters that used for the calculation of IRWQISC whereas DO, NO3, PO4, and BOD were used for the measurement of NSFWQI index. One way analysis of variance was applied for the comparison of the mean of water quality parameters among different years. Moreover, ArcGIS 10.1 was applied for spatial analysis of water quality parameters and water quality indexes.
Results: The results of this study indicated that according to IRWQISC index, Baghan and Dalaki rivers with the scores of 31.3 and 39.8 respectively, were categorized as low water quality rivers; while Shapour, Mond, Bahoosh, and Helleh with the scores ranging between 46 to 53, were placed in the average water quality category. In addition, according to NSFWQI index, Baghan River with a water quality index value of 68 was in the average water quality category whereas the rest of the rivers with values ranging between 73 to 80 were placed in the high water quality category.
Conclusion: The findings of this study showed that the quality of water in Shapour, Dalaki, Helleh, Mond, Bahoosh and Baghan rivers was suitable for agricultural purposes. However, for drinking purposes, the water in these rivers should be purified.
Bushehr University of Medical Sciences
Iranian South Medical Journal
1735-4374
20
5
2017
11
1
Purification of Bysal Mussel (Modiolus sp. PG) Adhesive Protein fp-2 from Nortern Seashore of Persian Gulf
481
491
FA
Saba
Hosseini
Department of Marine Biology, School of Marine Science and Ocean, Khorramshahr University of Marine Science and Technology, Khorramshahr, Iran
Hossean
Zolgharnine
Department of Marine Biology, School of Marine Science and Ocean, Khorramshahr University of Marine Science and Technology, Khorramshahr, Iran
Afshar
Bargahi
The Persian Gulf Marine Biotechnology Research Center, The Persian Gulf Biomedical Sciences Research Institute, Bushehr University of Medical Sciences, Bushehr, Iran
Hoda
Khaledi
Department of Marine Biology, School of Marine Science and Ocean, Khorramshahr University of Marine Science and Technology, Khorramshahr, Iran
Negin
Salamat
Department of Marine Biology, School of Marine Science and Ocean, Khorramshahr University of Marine Science and Technology, Khorramshahr, Iran
Bita
Archangi
Department of Marine Biology, School of Marine Science and Ocean, Khorramshahr University of Marine Science and Technology, Khorramshahr, Iran
Background: Mussel bivalves produce and secrete adhesive proteins which facilitate the adherence to surfaces in the aquatic environments. Specific properties of these proteins such as adhesion, resistance and biocompatibility have introduced their application in biomedical engineering, dental, and production of biological adhesives.
Materials and Methods: The species of dwarves from the Persian Gulf were sampled based on the morphologic indexes and COI gene sequence. To identify the polyphenol glands, which produce the adhesive proteins, tissue sections were prepared from the mussel’s foot. Then, the mussel fp-2 protein was purified by the ion-exchange chromatography using salt and pH gradients and, the gel filtration chromatography. To confirm the presence of proteins in each fraction the Arnow test and NBT staining were used. For detection of the protein homogeneity, in each purification stage, the SDS-PAGE electrophoresis was used. The NBT positive chromatogram peaks, after concentration and electrophoresis by Acid-Urea method, were blotted on nitrocellulose and detected by NBT test.
Results: The specie morphology and taxonomy were recorded and matched to the FAO criteria and, "The Mollusks of Persian Gulf", the Persian reference book. It belonged to the Mytilidae family and Modiolus genus. The DNA and protein sequences were recorded in NCBI data bank as AB972412.1 and BAP16437.1 respectively. Also, the molecular weight of the purified fp-2 protein was about 45 KD.
Conclusion: This research was led to the morphologic and genetic identification of Modiolus sp. PG from the Persian Gulf along with the purification of the fp-2 adhesive protein.
Bushehr University of Medical Sciences
Iranian South Medical Journal
1735-4374
20
5
2017
11
1
Levofloxacin Resistance in Blood and Urine Culture Samples in Khalij Fars Hospital of Bushehr
492
500
FA
Fshemeh
Hadavand
The Persian Gulf Tropical Medicine Research Center, The Persian Gulf Biomedical Sciences Research Institute, Bushehr University of Medical Sciences, Bushehr, Iran<br>Department of Infectious Diseases, School of Medicine, Bushehr University of Medical Sciences, Bushehr, Iran
Katayon
Vahdat
The Persian Gulf Tropical Medicine Research Center, The Persian Gulf Biomedical Sciences Research Institute, Bushehr University of Medical Sciences, Bushehr, Iran<br>Department of Infectious Diseases, School of Medicine, Bushehr University of Medical Sciences, Bushehr, Iran
Sara
Yazdani
The Persian Gulf Tropical Medicine Research Center, The Persian Gulf Biomedical Sciences Research Institute, Bushehr University of Medical Sciences, Bushehr, Iran
Nilofar
Motamed
Department of Community Medicine, School of Medicine, Bushehr University of Medical Sciences, Bushehr, Iran <br>The Persian Gulf Nuclear Medicine Research Center, The Persian Gulf Biomedical Sciences Research Institute, Bushehr University of Medical Sciences, Bushehr, Iran
Background: Due to the broad-spectrum antimicrobial activity of Levofloxacin, it has been used widely around the world. Recently, levofloxacin-resistance reports have been published. In this study, we investigated resistance to levofloxacin in positive urine and blood culture samples in Persian Gulf hospital in Bushehr, Iran, during 2015-16.
Materials and Methods: In this cross-sectional study, the selection criteria included all positive urine or blood culture samples in which the amount of the isolated pathogen colony counts were more than 105. Culture samples were divided into three groups including sensitive, intermediate and resistant; based on bacterial growth around the discs. SPSS version 18.0 was used as the statistical analysis software, and a p-value of less than 0.05 was considered statistically significant.
Results: Culture samples consisted of samples of150 patients including 61 (%40.7) male and 89 (%59.3) female. Mean age of participants was 42.98 ± 29.25. Culture samples consisted of urine (% 50.7) and blood cultures (% 49.3). E.coli was the most common pathogen (% 46) and Klebsiella (% 16.7) was the second common pathogen in all cultures. Regarding the sensitivity to levofloxacin, 119 (% 79.3) samples were sensitive, 22 (% 14.7) cultures had intermediate sensitivity and 9 (%6) samples were resistant to levofloxacin. The only resistant pathogen was E.coli.
Conclusion: This study showed that Levofloxacin has a reasonably high efficiency against most of the bacterial pathogens except for the E.coli that showed some resistance. Hence, this antibiotic can still be a considered as a good choice in the treatment of most infections except E.coli
Bushehr University of Medical Sciences
Iranian South Medical Journal
1735-4374
20
5
2017
11
1
A Roadmap Draft for the Development of Synthetic Biology in I.R. Iran
501
518
FA
Iraj
Nabipour
The Persian Gulf Marine Biotechnology Research Center, The Persian Gulf Biomedical Sciences Research Institute, Bushehr University of Medical Sciences, Bushehr, Iran<br>Future Studies Group, The Academy of Medical Sciences of the I.R. Iran
Background: According to the Royal Academy of Engineering (2009), “synthetic biology aims to design and engineer biologically-based parts, novel devices, and systems as well as redesigning existing, natural biological systems”. It has been predicted that synthetic biology would be one of twelve destructive and creative technologies in the future and would transform the life, business, and economic world.
Methods: By considering critical infrastructures in biological sciences, biomedical research centers, biotechnological infrastructures, national innovation system, national scientific roadmap and centers for doctoral training in the I.R. Iran and recent experiences for the progress of synthetic biology in different countries, a conceptual framework for the development of synthetic biology in the I.R. Iran was designed.
Results: Five strategies may be considered to develop synthetic biology in the I.R. Iran. 1. The establishment of centers of excellence for research in synthetic biology; 2. Support of multidisciplinary cores for biomedical technologies in universities; 3. Networking and building a national Smart Specialization Platform for synthetic biology; 4. Education and training in synthetic biology to provide the critical national workforce mass; 5. Public engagement with synthetic biology.
Conclusion: Together these five strategies provide an important foundation for the establishment of essential infrastructures and expert workforce for the development of synthetic biology in the I.R. Iran. By conducting a practical roadmap, Iran would be a leading nation in synthetic biology in the west part of Asia in the future.