57 1735-4374 Bushehr University of Medical Sciences 1327 Urogenital System Synergic Effect of TC99-m Gamma Radiation and Non-ionizing Radiation of Wi-Fi on Count, Morphology and Motility of Sperms in Rats: An Experimental Study Gheisari Farshid b Atefi Mohammad c Masoumirad Aref d b Ionizing and Non-Ionizing Radiation Protection Research Center (INIRPRC), School of Paramedical Sciences, Shiraz University of Medical Sciences, Shiraz, Iran c Ionizing and Non-Ionizing Radiation Protection Research Center (INIRPRC), School of Paramedical Sciences, Shiraz University of Medical Sciences, Shiraz, Iran d Ionizing and Non-Ionizing Radiation Protection Research Center (INIRPRC), School of Paramedical Sciences, Shiraz University of Medical Sciences, Shiraz, Iran 1 10 2020 23 4 280 291 26 03 2019 07 07 2020 Background: Given the effects of ionizing radiation on biological tissues and their irreversible tissue damage, this project aimed to determine the synergic effect of TC99-m gamma radiation and non-ionizing radiation of Wi-Fi on sperm characteristics in rats. Materials and Methods: Sixty adult male rats, weighing 250-200 g randomly divided into four groups (three experimental groups and one control group). The first experimental group received TC99-m through intraperitoneal injection. The second experimental group was exposed to Wi-Fi radiation daily for one hour in one week. The third experimental group received TC99-m and wireless simultaneously. Data were analyzed in SPSS v. 20. Results: Sperm count, motility and normal morphology significantly decreased respectively in Wi-Fi group, TC99-m group and TC99-m + Wi-Fi group (p <0.001) compared to the control group. The three experimental groups were significantly different (p <0.05). The highest reduction rate in sperm count, motility and normal morphology was observed in TC 99-m + Wi-Fi group (p < 0.05). Conclusion: Based on our results, if glands and other sensitive parts of animals are placed close to electromagnetic wave sources, their normal function will be disturbed.    
1328 Microbiology and Immunology Accuracy of Indirect ELISA Prepared from Recombinant Bp26 Gene of Brucella melitensis in Diagnosis of Human Brucellosis Hoseini Seyed Davood e Ghaznavirad Ehsanollah f Farazi Ali Asghar g e Department of Molecular Biotechnology, Razi Vaccine and Serum Research Instutute, Agricultural, Research Education and Extension Organization (AREEO), Arak Branch, Arak f Department of Clinical Microbiology, School of Medicine, Arak University of Medical Sciences, Arak, Iran g Department of Infectious Diseases, School of Medicine, Arak University of Medical Sciences, Arak, Iran 1 10 2020 23 4 292 301 13 06 2019 30 05 2020 Background: Considering the prevalence of brucellosis in Iran, it is necessary to choose a specific and sensitive laboratory method to diagnose it in a rapid and timely manner. The aim of this study was to assess the accuracy of indirect enzyme-linked immunosorbent assay (ELISA) for detecting brucellosis in humans in order to have an appropriate alternative to conventional tests such as Wright, 2ME, and commercial ELISA kits. Materials and Methods: In this study, the recombinant protein produced from the gene (omp28) bp26 Brucella melitensis was used as an antigen for coating microplate wells. A total of 124 serum samples of normal healthy individuals (n=62) and patients with acute brucellosis (n=62) approved by STA and 2 ME tests were entered into the study. The data were analyzed in SPSS (ver.18). Results: The mean age was 39.8±13.5 years in the patient group and 36.1±12.7 years in the healthy group. Furthermore, 66.1% of the patients were male and 62.9% lived in rural regions, while these figures were respectively 71% and 45.2% in the healthy group. The sensitivity of 92% and specificity of 87% and a positive predictive value of 88% and a negative predictive value of 92% and an accuracy of 90% were determined for ELISA kit used in this study. Conclusion: The ELISA diagnostic kit reacted to most of the positive human sera. However, this kit needs to be further evaluated with a larger sample size of clinical specimens from different regions and with various clinical forms of human brucellosis. 1329 Nervous System Effect of Transcranial Alternating Current Stimulation on Anxiety and Hemodynamic Response in Patients Undergoing Surgery: A Double-Blind Controlled Clinical Trial Arabzadeh Taeibeh h Mirhosseini Hamid i Sheikhi Mohammad Ali j h Department of Operating Room, School of Allied Medical Sciences, Shahid Sadoughi University of Medical Scienc-es, Yazd, Iran i Research Center of Addiction and Behavioral Sciences, Shahid Sadoughi University of Medical Sciences, Yazd, Iran j Department of Anesthesia and intensive care, Shahid Sadoughi University of Medical sciences, Yazd, Iran 1 10 2020 23 4 302 311 17 09 2019 29 04 2020 Background: Pre-op anxiety has adverse effects on the patient's anesthesia and treatment. Currently, electrical interventions are widely used as non-invasive methods to control pre-op anxiety and may have advantages as compared with medical therapies. This study aimed to determine the effect of transcranial alternating current stimulation on anxiety and hemodynamic response of patients undergoing surgery. Materials and Methods: This study enrolled 50 patients undergoing surgery with anesthesia class (Ι). Before surgery, the experimental group received a transcranial alternating current stimulation for 20 min. The control group underwent a sham electrical stimulation. The post-test was done 2 hr after the intervention and before surgery. The independent t-test, Chi-square and Kolmogorov-Smirnov tests were used to compare groups. The data were analyzed by SPSS v.16. Results: There were no significant differences in demographic variables between the groups. Patients’ anxiety level was not significantly different before the intervention between the groups. However, anxiety level of the experimental group was less than that of the control group after the intervention (P= 0.005). The pre-op systolic blood pressure and heart rate were less in the experimental group as compared with the control group (respectively P<0.001, P=0.001). A positive and significant correlation was observed between two variables of anxiety level and the pre-op need for information (P=0.00). Conclusion: The results of the present study indicated that transcranial alternating current stimulation is effective on reducing pre-op anxiety and stability of hemodynamic response of patients. Furthermore, training patients and furnishing them with information before surgery can reduce their anxiety. 1330 Microbiology and Immunology The Efficacy of Autologous Plasma Therapy in Patients with Chronic Urticaria Shirkani Afshin k Hadipour Abbas l Ostovar Afshin m Movahed Ali n Pordel Safora o Zamani Marjan p Shamshrikar Milad Omrani Fatemeh Farrokhi Shokroolah k Department of Immunology, Asthma and Allergy, The Persian Gulf Tropical Medicine Research Center, The Persian Gulf Biomedical Sciences Research Institute, Bushehr University of Medical Sciences, Bushehr, Iran l Department of Dermatology, The Persian Gulf Tropical Medicine Research Center, The Persian Gulf Biomedical Sciences Research Institute, Bushehr University of Medical Sciences, Bushehr, Iran m Department of Epidemiology, The Persian Gulf Tropical Medicine Research Center, The Persian Gulf Biomedical Sciences Research Institute, Bushehr University of Medical Sciences, Bushehr, Iran n Department of Biochemistry, The Persian Gulf Tropical Medicine Research Center, The Persian Gulf Biomedical Sciences Research Institute, Bushehr University of Medical Sciences, Bushehr, Iran o Department of Immunology, Asthma and Allergy, The Persian Gulf Tropical Medicine Research Center, The Persian Gulf Biomedical Sciences Research Institute, Bushehr University of Medical Sciences, Bushehr, Iran p Department of Immunology, Asthma and Allergy, The Persian Gulf Tropical Medicine Research Center, The Persian Gulf Biomedical Sciences Research Institute, Bushehr University of Medical Sciences, Bushehr, Iran Department of Immunology, Asthma and Allergy, The Persian Gulf Tropical Medicine Research Center, The Persian Gulf Biomedical Sciences Research Institute, Bushehr University of Medical Sciences, Bushehr, Iran Department of Immunology, Asthma and Allergy, The Persian Gulf Tropical Medicine Research Center, The Persian Gulf Biomedical Sciences Research Institute, Bushehr University of Medical Sciences, Bushehr, Iran Department of Immunology, Asthma and Allergy, The Persian Gulf Tropical Medicine Research Center, The Persian Gulf Biomedical Sciences Research Institute, Bushehr University of Medical Sciences, Bushehr, Iran 1 10 2020 23 4 312 320 19 01 2020 03 05 2020 Background: Chronic urticaria is an allergic skin disease characterized by severe pruritic wheal persisting at least for 6 weeks. This study aimed to evaluate the efficacy of plasma therapy used for the first time for the treatment of idiopathic chronic urticaria. Materials and Methods: In this case series study, the quality of life (QOL) of 30 patients with idiopathic chronic urticaria (autoimmune and spontaneous) referred to allergy clinic was evaluated before and after autologous plasma therapy by using standard questionnaires (DLQI and TSS). Results: The findings showed that the means of DLQI and TSS scores in the patients after plasma therapy was significantly reduced. The findings showed that the means of TSS scores in the patients after plasma therapy was significantly reduced (p<0/001). Moreover, it was found that plasma therapy was significantly effective patients with idiopathic chronic urticaria (autoimmune and spontaneous) by using standard questionnaires (TSS)(p=0.001). The findings showed that the means of DLQI  scores in the patients after plasma therapy was significantly reduced (p=<0.001) means of DLQI  scores in the women in contrast to men after plasma therapy was significantly reduced ( p=0.04) .This indicates that the improvement of the QOL in the patients. Moreover, it was found that plasma therapy was significantly effective in both sexes. Conclusion: The results of the present study suggest that autologous plasma therapy could be a suitable method for the treatment of patients with idiopathic chronic urticaria. 1331 Endocrine System Developmental Skills of Children with and Without Congenital Hypothyroidism Rovshan Belghes Hassanzadeh Rad Afagh Dalili Satila Gholi Famian Ali Reza Department of Linguistics and Foreign Languages, School of Persian Literature and Foreign Languages, Payame Noor University, Tehran, Iran Department of Linguistics and Foreign Languages, School of Persian Literature and Foreign Languages, Payame Noor University, Tehran, Iran Pediatric Diseases Research Center, School of Medicine, Guilan University of Medical Sciences, Rasht, Iran Department of Linguistics and Foreign Languages, School of Persian Literature and Foreign Languages, Payame Noor University, Tehran, Iran 1 10 2020 23 4 321 329 04 03 2020 16 06 2020 Background: Congenital hypothyroidism is one of the most common endocrinology diseases in children. Given the importance of evaluating the normal growth and development and identifying growth and development abnormalities, this study aimed to evaluate the developmental skills of patients with congenital hypothyroidism and healthy children. Materials and Methods: This controlled cross-sectional study recruited 30 children with congenital hypothyroidism and 30 healthy children aged three to five years who presented to endocrine clinic of 17 Shahrivar Hospital in Rasht. Ages and Stages Questionnaire, with 5 developmental domains of communication, gross motor skills, fine motor skills, problem solving, and personal-social skills was completed. Results: Comparison of the two groups revealed no significant difference between the groups in the domains of gross motor, problem solving, and personal-social skills (P>0.05), while there was a significant difference between them in the domains of communication and fine motor skills (P<0.05). Although lower mean scores of all skills were noted in patients with congenital hypothyroidism, the scores were acceptable. Conclusion: Based on the results, it appears that addressing congenital hypothyroidism and its complications can affect children’s health.   1332 General Ethnopharmacology of Medicinal Plants in Tangestan county Abolzadeh Sepideh Vahdat Katayon Nabipour Iraj The Persian Gulf Tropical Medicine Research Center, The Persian Gulf Biomedical Sciences Research Institute, Bushehr University of Medical Sciences, Bushehr, Iran The Persian Gulf Tropical Medicine Research Center, The Persian Gulf Biomedical Sciences Research Institute, Bushehr University of Medical Sciences, Bushehr, Iran The Persian Gulf Marine Biotechnology Research Center, The Persian Gulf Biomedical Sciences Research Institute, Bushehr University of Medical Sciences, Bushehr, Iran 1 10 2020 23 4 330 345 09 06 2020 22 08 2020 Background: Ethnopharmacology is a very diverse approach to drug discovery that involves the observation, description, and experimental study of native drugs and their biological activities based on botany, chemistry, biochemistry, pharmacology, and other disciplines such as anthropology, archeology, history, and linguistics that helps discovering of  natural products with biological activity. The aim of this study was to identify medicinal plants that have a traditional use in the Tangestan region of Bushehr in the north of Persian Gulf. Materials and Methods: The medical uses of medicinal plants were gathered from 20 local informants by face to face interviews. The relative frequency of citation (FRC) and cultural importance (CI) indices were calculated. Results: A total of 109 medicinal plants belonging to 55 families were identified. Zataria multiflora, Descuraninia Sophia, Matricarria charmomilla, Achillea eriophora DC, Salvia mirzayanii, Plantago psyllium, Haplophyllum tuberculatum, Teucrium polium, Prunus amygdalus had the highest CI and FRC indices. The highest medical uses were for gastrointestinal problems, infectious diseases, gynecology and obstetrics, metabolic diseases, and skin problems. Conclusion: There is a wide variety of medicinal plants in the Tangestan region of Bushehr province which are used for  various diseases that are consistent with the traditional medicine uses that are common in Iran and had been reported in the international medical literature. However, in this region, a number of plants are used to treat diseases that are specific to this region and research on these medicinal plants can be the beginning of a way to discover new drugs in the therapeutic field. 1333 Practice of Medicine Hermeneutic Analysis of Transcendental Medicine Theory and its Application in Deep Medicine from the Perspective of Islamic Medicine Paradigm Dashtimanesh Gholam Reza The Persian Gulf Marine Biotechnology Research Center, The Persian Gulf Biomedical Sciences Research Insti-tute, Bushehr University of Medical Sciences, Bushehr, Iran 1 10 2020 23 4 346 370 11 06 2020 25 08 2020 The principles of Islamic medicine are still unknown. Although various studies about Islamic medicine have been carried out, the philosophical foundations and framework of the theorizing of it have not yet been elucidated. Some of theorists have explored transcendental medicine theory (TMT) and its application in deep medicine paradigm. For studying of theoretical framework of the TMT, the present article attempts to identify the principles and norms of Islamic medicine by studying the historical and semantic aspects of it and to introduce its paradigmatic dimensions. Finally, this article studies the position of TMT in the paradigm of Islamic medicine. Historical and semantic methods have been used to carry out this research. The hermeneutic approaches and the interpretation of the text have also been used to describe the theoretical framework of TMT. The results of this study show that the Muslim physician has always tried to use the latest and most effective medical tools, methods and principles based on scientific approach within the ethical and religious foundations of religion, and has always used the most material elements of medicine, and uses a spiritual and divine interpretation of essence of that elements and all of the cosmos. 1334 General Toxinology of Venomous Marine Worms; A Review Mohebbi Gholam Hossian Maryamabadi Ammar Nabipour Iraj The Persian Gulf Marine Biotechnology Research Center, The Persian Gulf Biomedical Sciences Research Institute, Bushehr University of Medical Sciences, Bushehr, Iran The Persian Gulf Marine Biotechnology Research Center, The Persian Gulf Biomedical Sciences Research Institute, Bushehr University of Medical Sciences, Bushehr, Iran The Persian Gulf Marine Biotechnology Research Center, The Persian Gulf Biomedical Sciences Research Institute, Bushehr University of Medical Sciences, Bushehr, Iran 1 10 2020 23 4 371 430 06 04 2020 09 05 2020 Although the widespread distribution of venomous marine worms around the world, the structural and toxinologcal studies of their toxins are still limited.This study was aimed to evaluate the toxicity of poisonous marine worms. Touching the cilium of Chloeia flava and Sipuncula marine worms cause painful inflammation of the skin. Many species of nemertines prey their victims by a flexible proboscis and dense mucus on the surface of the skin. They have recently been classified into three groups of the Palaeonmertea, Pilidiophora, and Hoplonemertea. Their toxins comprise the three main groups of peptides, pyridine alkaloids, and tetradotoxin and their derivatives. The nicotinoide amphiporine compound, anabaseine and its derivatives, Nemertelline, and anabasine are as alkaloid toxins. They are selectively stimulated the  nicotinic receptors, in particular the type of α-7 receptors. Nemerteans are produced a variety of peptide toxins.The hemolytic activity is the most prominent activity of the cerebratulus toxins A-I to A-IV. Cytotoxin A-III, by forming tetrameric forms in the membranes of many different cells, and creating large pores, makes them very permeable. The toxin inhibits the protein kinase C and the selective cationic sodium and calcium channels of the voltage-gate in the nervous and cardiovascular systems at low lytic concentrations. From four main proteins "Cerebratulus toxins- B-II to B-IV", the toxin B-II performed to be the most toxic compound.The assessment of the Lineus extract showed that they are actually more toxic than Cerebratulus extracts. The neurotoxins α- and β-nemertides, respectively, belong to the family of three and six kilodaltons. The nemertide α-1 is highly potent toxin against the VGSC. Dose-dependent injections have caused permanent paralysis to death. Many toxin genes have been predicted or discovered in the genomes and transcriptoms of nemerteans. The most prominent of the toxin genes were the Plancitoxin-1 sequence, Stichodactyla toxin peptide (Shk toxin), Se-Cephalotoxin, β pore-forming toxin aerolysin, and several neurotoxin-related sequences, such as delta-actitoxin-Amc1a, Perivitellin-2, Mu-theraphotoxin Hhn2a 4 and turripeptide Gsg9.2, different α-, β-nemertides (B-neurotoxins analogs), and Parborlysin. Most likely, the Parborlysins belong to the same family of cytotoxins AI-AIV. Study on these marine organism toxins can provide useful probes for examining receptors, ion channels, and even innate immunity mechanisms against infectious viruses, parasites, and other microbes.