2024-03-29T02:37:10+03:30 http://ismj.bpums.ac.ir/browse.php?mag_id=70&slc_lang=fa&sid=1
70-1171 2024-03-29 10.1002
Iranian South Medical Journal Iran South Med J 1735-4374 1735-6954 10.52547/ismj 2019 22 5 In-vitro Apoptotic Effects of Deferoxamine on the Glioblastoma Cell Line Mahtab Pourkamalzadeh Seyid Mesam Abtahi froushani sm.abtahi@urmia.ac.ir Background: Research suggests the inhibitory effects of deferoxamine as an iron chelator on erythroleukemia cells. The present study was conducted to investigate the effects of deferoxamine on B92 cells as a model of glial cells carcinoma. Materials and Methods: The present experimental study treated 6×104 B92 cells with 0, 10, 50 and 100 µM of deferoxamine for 24 hours in the presence or absence of 10 μmol/l of ferric chloride. Morphological changes were evaluated in the treated cells compared to in the control sample using an inverse optical microscope. The inhibitory and cytotoxic effects of deferoxamine were evaluated using the dimethylimidazole-diphenyl tetrazolium bromide (MTT) reduction and neutral red uptake assay. The data were analyzed using the Kruskal-Wallis test. P <0.05 was set as the level of statistical significance. Results: The inhibitory effects of deferoxamine on the proliferation of B92 cells were identified after 24 hours in a way that the cells began to accumulate in the presence of deferoxamine. Ten μmol/l of ferric chloride prevented these morphological changes. Deferoxamine was also found to significantly and  dose-dependently inhibit the vitality and viability of B92 cells. Moreover, the data showed that ferric chloride prevents the emergence of the effects of treating B92 cells with deferoxamine. Conclusion: Deferoxamine exerts in-vitro antiproliferative effects on the glial cell line B92. Cell proliferation iron chelating agents glioma B92 cells 2019 12 01 264 277 http://ismj.bpums.ac.ir/article-1-1171-en.pdf 10.29252/ismj.22.5.264
70-1172 2024-03-29 10.1002
Iranian South Medical Journal Iran South Med J 1735-4374 1735-6954 10.52547/ismj 2019 22 5 Immunophenotypic and Immunotypic Evalua-tions of Human Myeloma Cell Lines KMM-1, JJN3, LP1, L363, KMS-12BM and RPMI-8226 for Cell-Line Authentication Nader Vazifeh shiran nadershirani@yahoo.com Saeid Abroun Background: The disease-associated cell lines used in the majority of preclinical trials should be first authenticated and identified to avoid directing research expenditure on wrong cells and obtaining irrelevant results. The present study evaluated the immunophenotypic and immunotypic authenticity of six human myeloma cell lines (HMCLs). Materials and Methods: Cytospin smear and Wright staining were used for invetigating cell morphology, the flowcytometry of the markers CD45, CD2, CD19, CD38 and CD138 for immunophenotypic investigations, capillary electrophoresis for the immunoglobulin secretion potency and immunofixation for the immunotypical investigation of the cells. Results: Given the definite plasma cell morphology, the identity of all the cell lines was confirmed, although a similar morphology was observed in L363 and JJN3. In addition to authenticating the myeloma nature of all the cell lines, immunophenotypic investigations suggested a complete similarity between L363 and JJN3 cell lines. Examining their immunotypes for a final confirmation found KMS12BM to be non-secretor, KMM1 to be BJP-λ and the remaining to be IgG-λ. Conclusion: Despite confirming the identity of four cell lines, i.e. RPMI-8226, KMS-12BM, KMM1 and LP1, that of JJN3 and L363 cells, whose immunotype was determined as IgGλ, was questioned owing to their fundamental morphological, immunophenotypic and immunotypic similarities. On the other hand, a reveiew of literature and ATCC.org suggested that the main nature of JJN3 is IgAκ. The authenticity of the JJN3 cell line was therefore unclear and it was indeed the same as that of L363. The JJN3 cell-line was therefore discarded, and the associated results were interpreted as consistent with the results of L363. Cell line HMCL myeloma authentication immunophenotype immunotype 2019 12 01 278 295 http://ismj.bpums.ac.ir/article-1-1172-en.pdf 10.29252/ismj.22.5.278
70-1173 2024-03-29 10.1002
Iranian South Medical Journal Iran South Med J 1735-4374 1735-6954 10.52547/ismj 2019 22 5 The Correlation of the Follicular Fluid Levels of Anti-Mullerian Hormone and Beta Human Chorionic Gonadotropin with Oocyte Matura-tion Afsaneh Ghasemi Zahra Doroudian Zhr.dorodian@gmail.com Maryam Barekat Fatemeh Sadat Amjadi Maryam Tabatabaee Background: Follicular fluid (FF) plays a key role in preparing a microenvironment for the development and fertilization of oocytes. Moreover, biochemical and hormonal changes in the FF surrounding the oocyte can affect its quality and successful fertilization. The present study was conducted to investigate the relationship of beta human chorionic gonadotropin (beta-HCG) and anti-mullerian hormone (AMH) levels of FF with oocyte maturation in in-vitro fertilization (IVF) cycles. Materials and Methods: FF was monitored in 30 women under a controlled ovarian stimulation cycle. Oocyte maturation was examined by an embryologist. Beta-HCG and AMH levels were then investigated in the FF containing metaphase I (MI), metaphase II (MII) and germinal vesicle (GV) oocytes using ELISA. To evaluate clinical pregnancy, serum levels of beta-HCG were measured 14 days after transfering the embryo. Identifying fetal heart rate (FHR) was considered clinical pregnancy. Results: No significant differences were observed in the mean betal-HCG levels between the mature (53.73±21.081 IU/ml) and immature oocytes (52.90±21.000 IU/ml) (P=0.8). Beta-HCG levels of the FF containing the MII oocytes (64.73±14.49 IU/ml) were significantly related to clinical pregnancy (P=0.04). Oocyte maturation and clinical pregnancy were also found not to be significantly related to AMH levels of FF. Conclusion: The present study found oocyte maturation not to be significantly associated with the beta-HCG and AMH levels of FF. Beta-HCG levels of FF were also found to be associated with clinical pregnancy. The other constituents of FF along with AMH and beta-HCG might have therefore affected the oocyte quality and maturation.   Oocyte maturation beta-HCG AMH IVF FF 2019 12 01 296 306 http://ismj.bpums.ac.ir/article-1-1173-en.pdf 10.29252/ismj.22.5.296
70-1174 2024-03-29 10.1002
Iranian South Medical Journal Iran South Med J 1735-4374 1735-6954 10.52547/ismj 2019 22 5 Comparing the Prophylactic Effect of Pregabalin with that of Aminophylline Plus Dexamethasone on Postdural Puncture Headache after Spinal Anesthesia in Cesarean Section: A Double-Blind Controlled Randomized Clinical Trial Abdolreza Najafi Anaraki Kamran Mirzaei Kamran.mirzaei@gmail.com Background: Headache is yet considered an undesirable complication of spinal anesthesia as a selective method in cesarean section. The present study was conducted to compare the prophylactic effect of pregabalin with that of aminophylline plus dexamethasone on postdural puncture headache after spinal anesthesia in cesarean section. Materials and Methods: The present double-blind controlled randomized clinical trial assigned 375 pregnant women as volunteers for elective cesarean section to three groups of 125 receiving pregabalin, aminophylline plus dexamethasone and placebo. Observational evaluation of the incidence and severity of headache was performed in these patients within the first 24 postoperative hours, i.e. 6, 12 and 24 hours after the surgery. Results: No statistically-significant differences were observed between the three groups receiving pregabalin, aminophylline plus dexamethasone and placebo in terms of the incidence and severity of headache 6, 12 and 24 hours after cesarean section performed under spinal anesthesia. Conclusion: The prophylactic administration of pregabalin or aminophylline plus dexamethasone does not affect postdural puncture headache after spinal anesthesia in cesarean section. pregabalin aminophylline dexamethasone headache spinal anesthesia caesarean section 2019 12 01 307 316 http://ismj.bpums.ac.ir/article-1-1174-en.pdf 10.29252/ismj.22.5.307
70-1175 2024-03-29 10.1002
Iranian South Medical Journal Iran South Med J 1735-4374 1735-6954 10.52547/ismj 2019 22 5 Dispersion and Health Risk Assessment of PM10 Emitted from the Stacks of a Ceramic and Tile industry in Ardakan, Yazd, Iran, Using the AERMOD Model Saeid Shojaee Barjoee said.shojaee71@gmail.com Hamidreza Azimzadeh Mohammadreza kuchakzadeh Asghar MoslehArani Hamid Sodaiezadeh Background: In developing countries, air pollution caused by industries constitutes a serious threat to public health. The present study was conducted to determine the dispersion patern and assess the health risks of PM10 emitted from the stacks of a ceramic and tile factory. Materials and Methods: The present descriptive-cross sectional study was performed on a tile and ceramic industry in Ardakan, Yazd, Iran. The stacks emission information and meteorological and topographical data were first prepared to run the AERMOD model and draw the disperesion patern and evaluate exposure to PM10. The simulated concentrations were then compared to EPA and WHO standards, and carcinogenic and non-carcinogenic health risks of exposure to PM10 were calculated using the formulas proposed by EPA. Results: The results showed unifrom PM10 dispersion in all directions given the flat modeling area. The simulated maximum PM10 concentrations were found to be higher than the maxium thresholds stipulated in both the standards for a 24-hour period and higher than the WHO thresholds on an annual basis. In contrast, the average daily and annual concentrations were found to be below the standard limits. The results of assessing both carcinogenic and non-carcinogenic health risks were therefore estimated to be acceptable.  Conclusion: Although the present study calculated the contribution of the study factory’s stacks to carcinogenic and non-carcinogenic health risks as acceptable, the cumulative effects of industries in Ardakan can increase these risks in the villages surrounding these industries. Exposure dispersion health risk assessment stack PM10 2019 12 01 317 332 http://ismj.bpums.ac.ir/article-1-1175-en.pdf 10.29252/ismj.22.5.317
70-1176 2024-03-29 10.1002
Iranian South Medical Journal Iran South Med J 1735-4374 1735-6954 10.52547/ismj 2019 22 5 Antidepressant Effects of a Hydroalcoholic Extract of Rosa Canina L. on the Depression inDuced by Reserpine in Balb/c mice Rahmatollah Parandin rahmatparandin@pnu.ac.ir Background: Recent studies have shown that oxidative stress is a major cause of depression. Rosa canina L. is a fruit that contains different antioxidant compounds. The present study was conducted to investigate the antidepressant effects of a hydroalcoholic extract of Rosa canina L. on male mice using the behavioral tests of forced swim test (FST) and tail suspension test (TST). Materials and Methods: The present experimental study randomly assigned 42 mice to 6 groups of 7, i.e. the control (normal saline), the reserpine (negative control), the fluoxetine (positive control) and three reserpine groups intraperitoneally treated with 100, 200 and 400 mg/kg of the hydroalcoholic extract of Rosa canina L. Depression was evaluated using the FST and TST. The total antioxidant capacity (TAC) and malondialdehyde (MDA) levels of the brain were also measured. The data were analyzed in SPSS using One-Way ANOVA. P<0.05 was set as the level of stastistical significance. Results: The FST and TST showed that reserpine significantly increased the duration of immobility. The immobility duration was significantly (P<0.001) reduced by 400 mg/kg of the extract in the two behavioral tests. Reserpine also significantly decreased the TAC and increased MDA levels in the brain. The TAC was significantly increased and MDA levels significantly decreased by 200 mg/kg (P<0.05) and 400 mg/kg (P<0.001) of the extract. Conclusion: The present findings confirmed the antidepressant activity of Rosa canina L. potentially mediated by its antioxidant components and resembling that of fluoxetine . FST TST oxidative stress reserpine antidepressant mice Rosa canina L. 2019 12 01 333 346 http://ismj.bpums.ac.ir/article-1-1176-en.pdf 10.29252/ismj.22.5.333
70-1177 2024-03-29 10.1002
Iranian South Medical Journal Iran South Med J 1735-4374 1735-6954 10.52547/ismj 2019 22 5 Ethical Debates on Synthetic Biology Amirreza Bolkheir Iraj Nabipour inabipour@gmail.com Background: As an emerging interdisciplinary area of science with a multitude of potential facilities and applications, synthetic biology integrates different disciplines with one another ranging from basic science to engineering. This interdisciplinary branch therefore encompasses sciences that require specific development and ethical approaches. Materials and Methods: Science management principles were used to design the framework of discrete ethical issues of synthetic biology in terms of the creation, storage, distribution and application of synthetic biology. Results: There is a debate over whether or not the artificial organisms created as living machines deserve ethical considerations. The free storage of knowledge as open libraries about manufacturing the parts, devices and biological systems is another issue. In this respect, technological gap and justice could also be debated. Biosafety and biosecurity are also important in applying synthetic biology, as this science is increasingly facing the community of garage biologists. In biosecurity, creating organisms with dual applications raises ethical issues regarding bioterrorism. Conclusion: The complexity and broad scope of ethical issues regarding the application of synthetic biology products require that distinct ethical principles be developed in collaboration with experts in ethics and law and futurists in discourse with the civil community. Synthetic biology biosecurity biosafety biological ethics bioterrorism 2019 12 01 347 363 http://ismj.bpums.ac.ir/article-1-1177-en.pdf 10.29252/ismj.22.5.347
70-1178 2024-03-29 10.1002
Iranian South Medical Journal Iran South Med J 1735-4374 1735-6954 10.52547/ismj 2019 22 5 Primary Cutaneous Mucormycosis of the Neck:A CaseReport with Review of Literature Reza kaboodkhani Amirreza Bolkheir bolkhayramirreza@yahoo.com Hamideh Zaree Katayon Vahdat Background: Cutaneous mucomycosis is an invasive fungal infection that usually develops in the immunodeficient patients. Although it also occurs in cases without underlying disease. So far in Iran and the world, primary cutaneous mucormycosis of the neck without predisposing factor has not  been reported. Materials and Methods: In this case,we present a 51 years old patient with progressive neck swelling and skin necrosis without any underlying disease. Firstly, treated as Ludwig Angina and necrotizing fasciitis. Finally diagnosed as neck mucormycosis according to pathologic result. systemic antifungal treatment and multiple surgical debridements with close intervals performed for him. Results: Primary cutaneous mucormycosis should be considered in any case of cutaneous necrosis ,even without underlying disease. A multidisciplinary approach is essential to improve survival rate of neck mucormycosis patients. Conclusion: Rapid diagnosis and initiation of systemic antifungal therapy along with invasive surgical debridement  can  improve survival rate and subsequent soft tissue reconstruction of  the neck mucormycosis in patients without underlaying disease. neck mucormycosis neck necrosis surgical debridement Amphotericin B 2019 12 01 364 370 http://ismj.bpums.ac.ir/article-1-1178-en.pdf 10.29252/ismj.22.5.364