Volume 20, Issue 5 (Iranian South Medical Journal 2017)
Iran South Med J 2017, 20(5): 416-425 |
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Mazoochi T, Karimian M, Mazoochi M, Hosseinzadeh Colagar A. Association of c.*49T>C and c.-932 G>A Polymorphisms of CYBA Gene with Coronary Artery Disease: a Case-Control Study in Kashan Population. Iran South Med J 2017; 20 (5) :416-425
URL: http://ismj.bpums.ac.ir/article-1-895-en.html
URL: http://ismj.bpums.ac.ir/article-1-895-en.html
1- Anatomical Sciences Research Center, Kashan University of Medical Sciences, Kashan, Iran
2- Anatomical Sciences Research Center, Kashan University of Medical Sciences, Kashan, Iran
Department of Molecular and Cell Biology, School of Basic Sciences, University of Mazandaran, Babolsar, Iran
3- Department of Cardiology, School of Medicine, Kashan University of Medical Sciences, Kashan, Iran
4- Department of Molecular and Cell Biology, School of Basic Sciences, University of Mazandaran, Babolsar, Iran
2- Anatomical Sciences Research Center, Kashan University of Medical Sciences, Kashan, Iran
Department of Molecular and Cell Biology, School of Basic Sciences, University of Mazandaran, Babolsar, Iran
3- Department of Cardiology, School of Medicine, Kashan University of Medical Sciences, Kashan, Iran
4- Department of Molecular and Cell Biology, School of Basic Sciences, University of Mazandaran, Babolsar, Iran
Abstract: (5018 Views)
Background: The CYBA (Cytochrome B-245 Alpha Chain; p22phox) gene encodes an essential subunit of NADH/NADPH-oxidase. This enzyme is expressed in smooth muscle cells of arteries which produces several active oxygen products. On the other hand, oxidative stress has a significant role in the pathogenesis of coronary artery disease (CAD). This study aimed to investigate the association between rs7195830 (c.*49T>C) and rs9932581 (c.-930G>A) polymorphisms in CYBA gene with coronary artery disease in an Iranian population.
Materials and Methods: In this case-control study, citrated blood samples were obtained from 180 participants, including 85 patient with CAD and 95 healthy volunteers. The fragments containing rs7195830 and rs9932581 of CYBA gene from extracted genome were amplified by polymerase chain reaction. Then the genotype of samples was detected using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. Genetic association analysis was performed using logistic regression test.
Results: Data analysis of c.-930G>A polymorphism revealed a significant association between AA genotype and risk of CAD (OR: 2.71, 95%CI: 1.04-7.06, p= 0.041). Also, the allelic analysis revealed that there was a significant association between A allele and CAD risk (OR: 1.65, 95%CI: 1.05-2.57, p=0.029), while there was no significant association between c.*49T>C polymorphism and risk of CAD.
Conclusion: The study results showed that the c.-930G>A polymorphism might be associated with the susceptibility to CAD and could be considered as a potential biomarker in future studies.
Materials and Methods: In this case-control study, citrated blood samples were obtained from 180 participants, including 85 patient with CAD and 95 healthy volunteers. The fragments containing rs7195830 and rs9932581 of CYBA gene from extracted genome were amplified by polymerase chain reaction. Then the genotype of samples was detected using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. Genetic association analysis was performed using logistic regression test.
Results: Data analysis of c.-930G>A polymorphism revealed a significant association between AA genotype and risk of CAD (OR: 2.71, 95%CI: 1.04-7.06, p= 0.041). Also, the allelic analysis revealed that there was a significant association between A allele and CAD risk (OR: 1.65, 95%CI: 1.05-2.57, p=0.029), while there was no significant association between c.*49T>C polymorphism and risk of CAD.
Conclusion: The study results showed that the c.-930G>A polymorphism might be associated with the susceptibility to CAD and could be considered as a potential biomarker in future studies.
Type of Study: Original |
Subject:
Cardiovascular System
Received: 2016/10/29 | Accepted: 2017/03/13 | Published: 2017/11/13
Received: 2016/10/29 | Accepted: 2017/03/13 | Published: 2017/11/13
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