Volume 22, Issue 1 (Iranian South Medical Journal 2019)
Iran South Med J 2019, 22(1): 16-28 |
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Sadeghi M, Vahedi Moghadam A, Movahed A. Effects of Alcoholic Extract of Portulaca Oleracea on Modulation of Neuropathic and Acute Pain in Rats. Iran South Med J 2019; 22 (1) :16-28
URL: http://ismj.bpums.ac.ir/article-1-1045-en.html
URL: http://ismj.bpums.ac.ir/article-1-1045-en.html
1- Department of Physiology, School of Medicine, Bushehr University of Medical Sciences, Bushehr, Iran , m.sadeghi@bpums.ac.ir
2- School of Medicine, Bushehr University of Medical Sciences, Bushehr, Iran
3- Department of Biochemistry, School of Medicine, Bushehr University of Medical Sciences, Bushehr, Iran
2- School of Medicine, Bushehr University of Medical Sciences, Bushehr, Iran
3- Department of Biochemistry, School of Medicine, Bushehr University of Medical Sciences, Bushehr, Iran
Abstract: (4062 Views)
Background: Portulaca Oleracea is a medicinal plant with many effects including analgesic and anti-inflammatory properties. This study aimed to investigate the effect of alcoholic extract of Portulaca Olerecea on CCI model of neuropathic pain (Bennet & Xie model) and acute thermal pain induced by Tail Flick.
Materials and Methods: Adult male Wistar rats weighing 200-250 g were used. Sensitivity to mechanical stimuli (mechanical allodynia) and noxious thermal stimuli were evaluated by Von Frey filaments and Tail Flick, respectively. In neuropathic pain studies, animals were randomly assigned to five groups of sham, CCI, two groups subjected to CCI and injected with extract (200 and 400 mg/kg, i.p.) and a group subjected to CCI and injected with normal saline. In these groups, mechanical allodynia was assessed on day 7 after surgery. In acute pain studies, animals were divided to three groups of a group that received normal saline and two groups that received extract (200 and 400 mg/kg, i.p.). In these groups, Tail Flick was measured 30 minutes after normal saline or extract administration. Data were analyzed by SPSS and appropriate statistical tests.
Results: All of the rats that had experienced CCI, exhibited mechanical allodynia after neuropathy.Portulaca oleracea could reduce the development of mechanical allodynia after CCI at 200 and 400 mg/kg doses. Thermal acute pain was reduced by 400 mg/kg of the extract.
Conclusion: Our findings indicate that Portulaca Oleracea extract can reduce behavioral symptoms of
neuropathic and acute pain.
Materials and Methods: Adult male Wistar rats weighing 200-250 g were used. Sensitivity to mechanical stimuli (mechanical allodynia) and noxious thermal stimuli were evaluated by Von Frey filaments and Tail Flick, respectively. In neuropathic pain studies, animals were randomly assigned to five groups of sham, CCI, two groups subjected to CCI and injected with extract (200 and 400 mg/kg, i.p.) and a group subjected to CCI and injected with normal saline. In these groups, mechanical allodynia was assessed on day 7 after surgery. In acute pain studies, animals were divided to three groups of a group that received normal saline and two groups that received extract (200 and 400 mg/kg, i.p.). In these groups, Tail Flick was measured 30 minutes after normal saline or extract administration. Data were analyzed by SPSS and appropriate statistical tests.
Results: All of the rats that had experienced CCI, exhibited mechanical allodynia after neuropathy.Portulaca oleracea could reduce the development of mechanical allodynia after CCI at 200 and 400 mg/kg doses. Thermal acute pain was reduced by 400 mg/kg of the extract.
Conclusion: Our findings indicate that Portulaca Oleracea extract can reduce behavioral symptoms of
neuropathic and acute pain.
Type of Study: Original |
Subject:
Pharmacology
Received: 2018/11/27 | Accepted: 2019/01/13 | Published: 2019/04/7
Received: 2018/11/27 | Accepted: 2019/01/13 | Published: 2019/04/7
References
1. Almeida RN, Navarro DS, Barbosa-Filho JM. Plants with Central Analgesic Activity. Phytomedicine 2001; 8(4): 310-22. [DOI:10.1078/0944-7113-00050]
2. Bahmani M, Shirzad H, Majlesi M, et al. A Review Study on Analgesic Applications of Iranian Medicinal Plants. Asian Pac J Trop Med 2014; 7(S1): S43-53. [DOI:10.1016/S1995-7645(14)60202-9]
3. Dworkin RH, Backonja M, Rowbotham MC, et al. Advances in Neuropathic Pain: Diagnosis, Mechanisms, and Treatment Recommendations. Arch Neurol 2003; 60(11): 1524-34. [DOI:10.1001/archneur.60.11.1524]
4. Perry VH, Hume DA, Gordon S. Immunohistochemical Localization of Macrophages and Microglia in the Aadult and Developing Mouse Brain. Neuroscience 1985; 15(2): 313-26. [DOI:10.1016/0306-4522(85)90215-5]
5. Ristoiu V. Contribution of Macrophages to Peripheral Neuropathic Pain Pathogenesis. Life Sci 2013; 93(23): 870-81. [DOI:10.1016/j.lfs.2013.10.005]
6. Qu L, Ma C. Animal Models of Pain After Peripheral Nerve Injury. In: Ma C, Zhang J-M, eds. Animal Models of Pain. Vol 49. Totowa, NJ: Humana Press, 2011, 69-80. [DOI:10.1007/978-1-60761-880-5_4]
7. Bridges D, Thompson SW, Rice AS. Mechanisms of Neuropathic Pain. Br J Anaesth 2001; 87(1): 12-26. [DOI:10.1093/bja/87.1.12]
8. Guindon J, Walczak JS, Beaulieu P. Recent Advances in the Pharmacological Management of Pain. Drugs 2007; 67(15): 2121-33. [DOI:10.2165/00003495-200767150-00002]
9. Rao J, Jayasree T, Mallikarjuna Rao B, et al. Evaluation of the Anti-Nociceptive and Anti-Inflammatory Activities of the Pet: Ether Extract of Portulaca Oleracea (Linn.). J Clin Diagn Res 2012; 6(2): 226-30.
10. Radhakrishnan R, Zakaria MN, Islam MW, et al. Neuropharmacological Actions of Portulaca Oleraceae L v. sativa (Hawk). J Ethnopharmacol 2001; 76(2): 171-6. [DOI:10.1016/S0378-8741(01)00230-6]
11. Rashed AN, Afifi FU, Disi AM. Simple Evaluation of the Wound Healing Activity of a Crude Extract of Portulaca Oleracea L. (growing in Jordan) in Mus Musculus JVI-1. J Ethnopharmacol 2003; 88(2-3): 131-6. [DOI:10.1016/S0378-8741(03)00194-6]
12. Bennett GJ, Xie Y-K. A Peripheral Mononeuropathy in Rat that Produces Disorders of Pain Sensation like those Seen in Man. Pain 1988; 33(1): 87-107 [DOI:10.1016/0304-3959(88)90209-6]
13. Chan K, Islam MW, Kamil M, et al. The Analgesic and Anti-Inflammatory Eeffects of Portulaca Oleracea L. subsp. Sativa (Haw.) Celak. J Ethnopharmacol 2000; 73(3): 445-51. [DOI:10.1016/S0378-8741(00)00318-4]
14. Karimi G, Khoei A, Omidi A, et al. Protective effect of aqueous and ethanolic extracts of Portulaca oleracea against cisplatin induced nephrotoxicity. Iran J Basic Med Sci 2010;13(2):31-5.
15. Le Bars D, Gozariu M, Cadden SW. Animal Models of Nociception. Pharmacol Rev 2001; 53(4): 597-652.
16. Zhou YX, Xin HL, Rahman K, et al. Portulaca Oleracea L.: A Review of Phytochemistry and Pharmacological Effects. Biomed Res Int 2015; 2015: 925631. [DOI:10.1155/2015/925631]
17. Mirabzadeh M, Rahimi R, Sahraee Z. Evaluation of Adverse Events Reported in Traditional Iranian Medicine Following Administration of Aqueous Extract of Herba Portulacae Oleraceae Seed. J Tradit Chin Med 2013; 33(4): 535-7. [DOI:10.1016/S0254-6272(13)60161-2]
18. Wang X, Traub RJ, Murphy AZ. Persistent Pain Model Reveals Sex Difference in Morphine Potency. Am J Physiol Regul Integr Comp Physiol 2006; 291(2): R300-6 [DOI:10.1152/ajpregu.00022.2006]
19. Calixto JB, Beirith A, Ferreira J, et al. Naturally Occurring Antinociceptive Substances from Plants. Phytother Res 2000; 14(6): 401-18.
https://doi.org/10.1002/1099-1573(200009)14:6<401::AID-PTR762>3.0.CO;2-H [DOI:10.1002/1099-1573(200009)14:63.0.CO;2-H]
20. Enna SJ, McCarson KE. The Role of GABA in the Mediation and Perception of Pain. Adv Pharmacol 2006; 54: 1-27. [DOI:10.1016/S1054-3589(06)54001-3]
21. Smith CG, Bowery NG, Whitehead KJ. GABA Transporter Type 1 (GAT-1) Uptake Inhibition Reduces Stimulated Aspartate and Glutamate Release in the Dorsal Spinal Cord in Vivo via Different GABAergic Mechanisms. Neuropharmacology 2007; 53(8): 975-81. [DOI:10.1016/j.neuropharm.2007.09.008]
22. Park JH, Han JB, Kim SK, et al. Spinal GABA Receptors Mediate the Suppressive Effect of Electroacupuncture on Ccold Allodynia in Rats. Brain Res 2010; 1322: 24-9. [DOI:10.1016/j.brainres.2010.02.001]
23. Nickel FT, Seifert F, Lanz S, et al. Mechanisms of Neuropathic Pain. Eur Neuropsychopharmacol 2012; 22(2): 81-91. [DOI:10.1016/j.euroneuro.2011.05.005]
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