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:: Volume 17, Issue 5 (Iranian South Medical Journal 2014) ::
Iran South Med J 2014, 17(5): 867-878 Back to browse issues page
Comparison of PCR with Standard Method (MPN) for detection of bacterial contamination in drinking water
Fatemeh Dehghan1, Mohammad Reza Zolfaghari2, Mohammad Arjomandzadegan 3, Somayeh Geravand4, GhplamReza Ahmari5, Salomeh Kalantari5, Ali Reza Kasravi6
1- Department Of Microbiology, Qom Branch, Islamic Azad University, Qom, IRAN
Hygiene and Quality Control Office of Markazi Province, Water and Wastewater Company, IRAN
2- Department Of Microbiology, Qom Branch, Islamic Azad University, Qom, IRAN
3- Tuberculosis and Pediatric Infectious Diseases Research Center, Arak University of Medical Sciences, Arak, IRAN , , mmatinam81@yahoo.com arjomandzadegan@arakmu.ac.ir
4- Tuberculosis and Pediatric Infectious Diseases Research Center, Arak University of Medical Sciences, Arak, IRAN
5- Hygiene and Quality Control Office of Markazi Province, Water and Wastewater Company, IRAN
6- Department of Microbiology, Science and Research Branch, Islamic Azad University, Arak, IRAN
Abstract:   (3614 Views)

Background: Detection of bacterial contamination in drinking water by culture method is a time and cost consuming method and spends a few days depending on contamination degree. However, the people use the tap water during that time. Molecular methods are rapid and sensitive. In this study a rapid Multiplex PCR method was used for rapid analysis both coliform bacteria and E.coli, and probable detection of VBNC bacteria in drinking water, the experiments were performed in bacteriological lab of water and Wastewater Corporation in Markazi province. Material and Methods:Amplification of a fragment from each of lacZ and uidA genes in a Multiplex PCR was used for detection of coliforms. Eight samples was taken from Arak drinking water system including 36 samples of wells, 41 samples of water distribution network and 3 samples from water storages were examined by amplification of lacZ and uidA genes in a Multiplex PCR. Equivalently, the MPN test was applied as a standard method for all samples for comparison of results. Standard bacteria, pure bacteria isolated from positive MPN and CRM were examined by PCR and MPN method. Results: The result of most samples water network, water storages, and water well were same in both MPN and PCR method .The results of standard bacteria and pure cultures of bacteria isolated from positive MPN and CRM confirmed the PCR method. Five samples were positive in PCR but negative in MPN method. Duration time of PCR was decreased about 105 min by changing the PCR program and electrophoreses factors. Conclusion: The Multiplex PCR can detect coliform bacteria and E.coli synchronous in drinking water.

Keywords: Comparison, PCR method, Bacterial contamination, Drinking water, MPN method
Full-Text [PDF 724 kb]   (1690 Downloads)    
Type of Study: Original | Subject: General
Received: 2014/10/15 | Accepted: 2014/10/15 | Published: 2014/10/15
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Dehghan F, Zolfaghari M R, Arjomandzadegan M, Geravand S, Ahmari G, Kalantari S et al . Comparison of PCR with Standard Method (MPN) for detection of bacterial contamination in drinking water. Iran South Med J. 2014; 17 (5) :867-878
URL: http://ismj.bpums.ac.ir/article-1-601-en.html


Volume 17, Issue 5 (Iranian South Medical Journal 2014) Back to browse issues page
دانشگاه علوم پزشکی بوشهر، طب جنوب ISMJ

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