Volume 20, Issue 2 (Iranian South Medical Journal 2017)
Iran South Med J 2017, 20(2): 170-179 |
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Gharibi S, Alebouyeh M, Falsafi T, Farzi N, Vaziri F, Zali M R. Protease Activity of Helicobacter Pylori Strains and their Association with Histopathological Changes of the Gastric Tissue. Iran South Med J 2017; 20 (2) :170-179
URL: http://ismj.bpums.ac.ir/article-1-869-en.html
URL: http://ismj.bpums.ac.ir/article-1-869-en.html
Somaye Gharibi1 
, Massoud Alebouyeh * 2, Tahereh Falsafi1 , Nastaran Farzi3 , Farzam Vaziri4 , Mohammad Reza Zali5
, Massoud Alebouyeh * 2, Tahereh Falsafi1 , Nastaran Farzi3 , Farzam Vaziri4 , Mohammad Reza Zali5
1- Department of Microbiology, School of Biological Science, Alzahra University, Tehran, Iran
2- Foodborne and Waterborne Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran ,masoud.alebouyeh@gmail.com
3- Foodborne and Waterborne Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran
4- Mycobacteriology and Pulmonary Research Department, Pasteur Institute of Iran
5- Gastroenterology and Liver Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran
2- Foodborne and Waterborne Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran ,
3- Foodborne and Waterborne Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran
4- Mycobacteriology and Pulmonary Research Department, Pasteur Institute of Iran
5- Gastroenterology and Liver Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Abstract: (5801 Views)
Background: Helicobacter pylori is the main cause of various gastroduodenal diseases. As the diseases caused by H. pylori are associated with severe tissue damage, proteases of bacteria may play an important roles in this process. In current study we investigated diversity of H. pylori protease activity and its association with different pathological lesions of gastric tissue.
Materials & Methods: The study was performed on 116 gastric biopsy specimens obtained from patients with gastrointestinal disorders referred to Taleghani hospital in Tehran, IR.Iran. Isolates were identified by culture and polymerase chain reaction (PCR). Their protease activity was assessed by spectrophotometric and well diffusion assays using casein as substrate. Variance analysis (One-way ANOVA) was used for statistical analysis.
Results: A prevalence of 43.1% (50/116) of H. pylori infection was detected in the studied patients. Histopathological lesions among 50 H. pylori positive patients were included: chronic gastritis 40% (20/50), intestinal metaplasia 8% (4/50), and severe active gastritis 52% (26/50). Nearly, 4.25% of the strains showed high protease activity, while 91.4% and 4.25% of the strains showed moderate and low protease activities, respectively. Statistical analysis demonstrated significant association between low protease activity of the strains and chronic gastritis (p= 0.044).
Conclusion: Results of this study indicated diversity of protease activity among different strains of
H. pylori and significant association between low protease activity of the strains and occurrence of chronic gastritis. Further studies are necessary to investigate this association.
Type of Study: Original |
Subject:
Microbiology and Immunology
Received: 2016/03/2 | Accepted: 2016/07/19 | Published: 2017/04/29
Received: 2016/03/2 | Accepted: 2016/07/19 | Published: 2017/04/29
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