:: Volume 15, Issue 2 (Iranian South Medical Journal 2012) ::
Iran South Med J 2012, 15(2): 85-92 Back to browse issues page
Differential comparison on protein components of the venoms obtained from two species of the Iranian endemic scorpions, Buthidae family
Alireza Farahmandzad *1, Rahim Sarvari2, Firooz Ebrahimi3, Mehdi Kamali2, Jamil Zargan3
1- Science Biology Research Center, Imam Hussein University, Tehran, IRAN , rezaf235@yahoo.com
2- Nanotechnology Research Center, Bghiatollah University of Medical Sciences, Tehran, IRAN
3- Science Biology Research Center, Imam Hussein University, Tehran, IRAN
Abstract:   (12283 Views)

Background: Two Dimensional Electrophoresis (2DE) is the most commonly and useful separation technique in proteomics. Each proteome snapshot becomes a protein profile. By means of this technique, several proteins are studied simultaneously. Methods: In this study, by use of (2DE) method, the differences of two profiles of Buthidae endemic scorpions, A.Crassicauda known as "black scorpion" and "O. doriae" yellow scorpion", were investigated. Results: For A.Crassicauda scorpion there were about 20 spots (peptides) in 6.2 - 8.2 pH ranges and molecular weight was less than 3 to 14 kDa and O. doriae scorpion 30 peptides, in 6.3 - 8.5 pH ranges, 1 to 45 kDa that fractionated and identified. Conclusion: By this method, the field of bioinformative data bank from Iranian endemic scorpions' venom could be prepared. By making change of any effective factors on scorpion venom, considerable results due to influence of the factor on determining kind of venom can be achieved and studied.

Keywords: scorpion, Buthidae, venoms, two dimensional electrophoresis
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Type of Study: Original | Subject: General
Received: 2010/11/27 | Accepted: 2011/02/20 | Published: 2012/03/17

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Volume 15, Issue 2 (Iranian South Medical Journal 2012) Back to browse issues page