1- Science Biology Research Center, Imam Hussein University, Tehran, IRAN , rezaf235@yahoo.com 2- Nanotechnology Research Center, Bghiatollah University of Medical Sciences, Tehran, IRAN 3- Science Biology Research Center, Imam Hussein University, Tehran, IRAN
Abstract: (12021 Views)
Background: Two Dimensional Electrophoresis (2DE) is the most commonly and useful separation technique in proteomics. Each proteome snapshot becomes a protein profile. By means of this technique, several proteins are studied simultaneously. Methods: In this study, by use of (2DE) method, the differences of two profiles of Buthidae endemic scorpions, A.Crassicauda known as "black scorpion" and "O. doriae" yellow scorpion", were investigated. Results: For A.Crassicauda scorpion there were about 20 spots (peptides) in 6.2 - 8.2 pH ranges and molecular weight was less than 3 to 14 kDa and O. doriae scorpion 30 peptides, in 6.3 - 8.5 pH ranges, 1 to 45 kDa that fractionated and identified. Conclusion: By this method, the field of bioinformative data bank from Iranian endemic scorpions' venom could be prepared. By making change of any effective factors on scorpion venom, considerable results due to influence of the factor on determining kind of venom can be achieved and studied.
Farahmandzad A, Sarvari R, Ebrahimi F, Kamali M, Zargan J. Differential comparison on protein components of the venoms obtained from two species of the Iranian endemic scorpions, Buthidae family. Iran South Med J. 2012; 15 (2) :85-92 URL: http://ismj.bpums.ac.ir/article-1-318-en.html
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