:: Volume 16, Number 6 (Iranian South Medical Journal 2014) ::
Iran South Med J 2014, 16(6): 394-400 Back to browse issues page
Evaluation of Rapid Urease Test Compared with Polymerase Chain Reaction for Diagnosis of Helicobacter pylori
Zahra Pakbaz1, Mohammad Hassan Shirazi *2, Mohammad Reza Pourmand1, Hossein Azhdarkosh3, Ziba Vaise Malekshahi4, Davood Afshar1, Sara . Hajikhani1, Nadia Mardani1
1- Department of Pathobiology, School of Health, Tehran University of Medical Sciences, Tehran, IRAN
2- Department of Pathobiology, School of Health, Tehran University of Medical Sciences, Tehran, IRAN , mhshirazi@tums.ac.ir
3- Department of Internal Training, School of Health, Tehran University of Medical Sciences, Tehran, IRAN
4- Department of Biotechnology, School of Sciences, Shahed University, Tehran, IRAN
Abstract:   (3622 Views)

Abstract Background: Current diagnostic methods for detecting Helicobacter pylori infection include rapid urease test (RUT), urea breath test (UBT), histology, culture, and polymerase chain reaction (PCR). RUT is the most commonly method to diagnose Helicobacter pylori infection because of its simple, rapid and accurate characters. The aim of this study is evaluation of rapid urease test compared with PCR for diagnosis of Helicobacter pylori. Material and Methods: In this study 94 patients with dyspeptic symptoms attending the endoscopy suite of gastroenterology section of the Firouzgar University Hospital, Tehran, Iran, were enrolled. Patient’s antrum biopsy specimens were collected at endoscopy for the rapid urease test and PCR. Data was recorded on a data sheet and analyzed using SPSS version 19.0. Sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV) of RUT was compared against PCR. The gold standard test for the diagnosis of Helicobacter pylori infection was PCR. Results: Sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV) of RUT respectively were 97.2%, 89.2%, 81.4% and 98%. Conclusion: RUT has high sensitivity and specificity for detection of Helicobacter pylori infection.

Keywords: Helicobacter pylori, rapid urease test, PCR, biopsy
Full-Text [PDF 255 kb]   (907 Downloads)    
Type of Study: Original | Subject: General
Received: 2013/12/17 | Accepted: 2013/12/17 | Published: 2013/12/17



PMID: 27284322
PMCID: PMC4887776


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Volume 16, Number 6 (Iranian South Medical Journal 2014) Back to browse issues page