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:: Volume 21, Issue 1 (Iranian South Medical Journal 2018) ::
Iran South Med J 2018, 21(1): 19-28 Back to browse issues page
Codon 72 Polymorphism of TP53 Gene in Cervical Biopsies with Abnormal Histology in Bushehr Province
Marzeah Zaker Hosseini 1, Sirous Naeimi 1, Maryam Moradinasab 2, Sakeneh Taherkhani 3, Fatemeh Farshadpour 4, Akram Ansarifar 4, Reza Taherkhani * 5
1- Department of Genetics, Colleague of Science, Kazerun branch, Islamic Azad University, Kazerun, Iran
2- The Persian Gulf Tropical Medicine Research Center, The Persian Gulf Biomedical Sciences Research Institute, Bushehr University of Medical Sciences, Bushehr, Iran
3- Reproductive Health and Midwifery Department, School of Nursing and Midwifery, Shahroud University of Medical Sciences, Shahroud, Iran
4- The Persian Gulf Marine Biotechnology Research Center, The Persian Gulf Biomedical Sciences Research Institute, Bushehr University of Medical Sciences, Bushehr, Iran
5- The Persian Gulf Tropical Medicine Research Center, The Persian Gulf Biomedical Sciences Research Institute, Bushehr University of Medical Sciences, Bushehr, Iran , taherkhanireza2005@yahoo.com
Abstract:   (1089 Views)
Background: TP53 tumor suppressor gene plays a role in repairing DNA damage as well as in cell apoptosis. This study was conducted to compare codon 72 polymorphism of TP53 gene between abnormal cervical tissue samples and samples of healthy women as a control group. In addition the frequency of human herpes simplex viruses (HSV) in these samples was determined.
Material and Methods: In this case-control study, 110 biopsies of the cervix with abnormal pathology were examined from archives of the Department of Pathology at Shohadaye Khalij Fars Hospital, and 164 healthy women were selected as the control group. PCR test was used for detection of HSV, and Allele-specific PCR was used for analyzing codon 72 polymorphism of TP53 gene. Data was analyzed in SPSS software.
Results: Distribution of codon 72 of TP53 genotypes in patient and control groups was not statistically significant. Evaluation of the frequency of arginine and proline alleles in the patient and control groups revealed that fewer patients were without proline allele (19%) compared to the control (30.1%), and this difference was statistically significant (p=0.04), (OR: 1.03-3.36، CI 95%=1.86). Furthermore, there was no significant correlation between TP53 genotype and variables of the pathology report, age, ethnicity and place of residence. In this study, HSV was not detected in the samples.
Conclusion: Because of the significant difference in proline allele between the case and control groups, it appears that this allele is associated with the abnormal cervical pathology results in Bushehr Province.
 
Keywords: Polymorphism, TP53 gene, Herpes simplex virus, Allele-specific PCR
Full-Text [PDF 757 kb]   (303 Downloads)    
Type of Study: Original | Subject: Biochemistry. Cell Biology and Genetics
Received: 2018/02/26 | Accepted: 2018/02/26 | Published: 2018/02/26
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Zaker Hosseini M, Naeimi S, Moradinasab M, Taherkhani S, Farshadpour F, Ansarifar A et al . Codon 72 Polymorphism of TP53 Gene in Cervical Biopsies with Abnormal Histology in Bushehr Province. Iran South Med J. 2018; 21 (1) :19-28
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Volume 21, Issue 1 (Iranian South Medical Journal 2018) Back to browse issues page
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