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:: Volume 21, Issue 1 (Iranian South Medical Journal 2018) ::
Iran South Med J 2018, 21(1): 19-28 Back to browse issues page
Study of Codon 72 Polymorphism of TP53 Gene in Cervical Biopsies with Abnormal Histology in Bushehr Province
Marzeah Zaker Hosseini 1, Sirous Naeimi 1, Maryam Moradinasab 2, Sakeneh Taherkhani 3, Fatemeh Farshadpour 4, Akram Ansarifar 4, Reza Taherkhani * 5
1- Department of Genetics, Colleague of Science, Kazerun branch, Islamic Azad University, Kazerun, Iran
2- The Persian Gulf Tropical Medicine Research Center, The Persian Gulf Biomedical Sciences Research Institute, Bushehr University of Medical Sciences, Bushehr, Iran
3- Reproductive Health and Midwifery Department, School of Nursing and Midwifery, Shahroud University of Medical Sciences, Shahroud, Iran
4- The Persian Gulf Marine Biotechnology Research Center, The Persian Gulf Biomedical Sciences Research Institute, Bushehr University of Medical Sciences, Bushehr, Iran
5- The Persian Gulf Tropical Medicine Research Center, The Persian Gulf Biomedical Sciences Research Institute, Bushehr University of Medical Sciences, Bushehr, Iran , taherkhanireza2005@yahoo.com
Abstract:   (697 Views)
Background: TP53 tumor suppressor gene play a role in repairing DNA damages and also in cell apoptosis. This research have paid to the association between codon 72 polymorphism of TP53 gene in a variety of abnormal cervical tissue samples compared to samples of healthy women as a control group and also frequency of human herpes simplex viruses (HSV) in these samples.
Material and Methods: In this case-control study, 110 biopsies of the cervix with abnormal pathology were examined from archives of the Department of Pathology at Shohadaye Khalij Fars Hospital, and 164 healthy women were selected as control group. PCR test was used for detection of HSV, and Allele-specific PCR was used for analysis of codon 72 polymorphism of TP53 gene. Data was analyzed with SPSS software.
Results: Distribution of codon 72 of TP53 genotypes in patient and control groups was not statistically significant. Evaluation of the frequency of arginine and proline alleles in patient and control groups revealed that those without proline allele were lower in patients (19%) compared to controls (30.1%), and this difference was statistically significant (p=0.04), (OR: 1.03-3.36، CI 95%=1.86). Also there were not significant correlation between the various results of pathology, age, ethnicity and place of residence with the TP53 genotype. In this study, HSV was not detected in samples.
Conclusion: Because of significant difference in proline allele between the case and control groups, it seems that this allele is associated with the  abnormal cervical pathology results in Bushehr province.
Keywords: Polymorphism, TP53 gene, Herpes simplex virus, Allele-specific PCR
Full-Text [PDF 757 kb]   (202 Downloads)    
Type of Study: Original | Subject: Biochemistry. Cell Biology and Genetics
Received: 2018/02/26 | Accepted: 2018/02/26 | Published: 2018/02/26
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Zaker Hosseini M, Naeimi S, Moradinasab M, Taherkhani S, Farshadpour F, Ansarifar A et al . Study of Codon 72 Polymorphism of TP53 Gene in Cervical Biopsies with Abnormal Histology in Bushehr Province. Iran South Med J. 2018; 21 (1) :19-28
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Volume 21, Issue 1 (Iranian South Medical Journal 2018) Back to browse issues page
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