Volume 22, Issue 5 (Iranian South Medical Journal 2019)
Iran South Med J 2019, 22(5): 296-306 |
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Ghasemi A, Doroudian Z, Barekat M, Amjadi F S, Tabatabaee M. The Correlation of the Follicular Fluid Levels of Anti-Mullerian Hormone and Beta Human Chorionic Gonadotropin with Oocyte Matura-tion. Iran South Med J 2019; 22 (5) :296-306
URL: http://ismj.bpums.ac.ir/article-1-1173-en.html
URL: http://ismj.bpums.ac.ir/article-1-1173-en.html
Afsaneh Ghasemi1 
, Zahra Doroudian * 2, Maryam Barekat3 , Fatemeh Sadat Amjadi1 , Maryam Tabatabaee1
, Zahra Doroudian * 2, Maryam Barekat3 , Fatemeh Sadat Amjadi1 , Maryam Tabatabaee1
1- Shahid Akbarabadi Clinical Research Unit, School of Medicine, Iran University of Medical Sciences, Tehran, Iran
2- Shahid Akbarabadi Clinical Research Unit, School of Medicine, Iran University of Medical Sciences, Tehran, Iran , Zhr.dorodian@gmail.com
3- Department of Regenerative Biomedicine, Center for Stem Cell Biology and Technology, Royan Institute, Tehran, Iran
2- Shahid Akbarabadi Clinical Research Unit, School of Medicine, Iran University of Medical Sciences, Tehran, Iran , Zhr.dorodian@gmail.com
3- Department of Regenerative Biomedicine, Center for Stem Cell Biology and Technology, Royan Institute, Tehran, Iran
Abstract: (3634 Views)
Background: Follicular fluid (FF) plays a key role in preparing a microenvironment for the development and fertilization of oocytes. Moreover, biochemical and hormonal changes in the FF surrounding the oocyte can affect its quality and successful fertilization. The present study was conducted to investigate the relationship of beta human chorionic gonadotropin (beta-HCG) and anti-mullerian hormone (AMH) levels of FF with oocyte maturation in in-vitro fertilization (IVF) cycles.
Materials and Methods: FF was monitored in 30 women under a controlled ovarian stimulation cycle. Oocyte maturation was examined by an embryologist. Beta-HCG and AMH levels were then investigated in the FF containing metaphase I (MI), metaphase II (MII) and germinal vesicle (GV) oocytes using ELISA. To evaluate clinical pregnancy, serum levels of beta-HCG were measured 14 days after transfering the embryo. Identifying fetal heart rate (FHR) was considered clinical pregnancy.
Results: No significant differences were observed in the mean betal-HCG levels between the mature (53.73±21.081 IU/ml) and immature oocytes (52.90±21.000 IU/ml) (P=0.8). Beta-HCG levels of the FF containing the MII oocytes (64.73±14.49 IU/ml) were significantly related to clinical pregnancy (P=0.04). Oocyte maturation and clinical pregnancy were also found not to be significantly related to AMH levels of FF.
Conclusion: The present study found oocyte maturation not to be significantly associated with the beta-HCG and AMH levels of FF. Beta-HCG levels of FF were also found to be associated with clinical pregnancy. The other constituents of FF along with AMH and beta-HCG might have therefore affected the oocyte quality and maturation.
Materials and Methods: FF was monitored in 30 women under a controlled ovarian stimulation cycle. Oocyte maturation was examined by an embryologist. Beta-HCG and AMH levels were then investigated in the FF containing metaphase I (MI), metaphase II (MII) and germinal vesicle (GV) oocytes using ELISA. To evaluate clinical pregnancy, serum levels of beta-HCG were measured 14 days after transfering the embryo. Identifying fetal heart rate (FHR) was considered clinical pregnancy.
Results: No significant differences were observed in the mean betal-HCG levels between the mature (53.73±21.081 IU/ml) and immature oocytes (52.90±21.000 IU/ml) (P=0.8). Beta-HCG levels of the FF containing the MII oocytes (64.73±14.49 IU/ml) were significantly related to clinical pregnancy (P=0.04). Oocyte maturation and clinical pregnancy were also found not to be significantly related to AMH levels of FF.
Conclusion: The present study found oocyte maturation not to be significantly associated with the beta-HCG and AMH levels of FF. Beta-HCG levels of FF were also found to be associated with clinical pregnancy. The other constituents of FF along with AMH and beta-HCG might have therefore affected the oocyte quality and maturation.
Type of Study: Original |
Subject:
Gynecology
Received: 2019/02/5 | Accepted: 2019/09/14 | Published: 2019/12/1
Received: 2019/02/5 | Accepted: 2019/09/14 | Published: 2019/12/1
References
1. Revelli A, Canosa S, Bergandi L, et al. Oocyte Polarized Light Microscopy, Assay of Specific Follicular Fluid Metabolites, and Gene Expression in Cumulus Cells as Different Approaches to Predict Fertilization Efficiency after ICSI. Reprod Biol Endocrinol 2017; 15(1): 47. [DOI:10.1186/s12958-017-0265-2]
2. Cadoret V, Frapsauce C, Jarrier P, et al. Molecular Evidence that Follicle Development is Accelerated in Vitro Compared to in Vivo. Reproduction 2017; 153(5): 493-508. [DOI:10.1530/REP-16-0627]
3. Costa LO, Mendes MC, Ferriani RA, et al. Estradiol and Testosterone Concentrations in Follicular Fluid as Criteria to Discriminate between Mature and Immature Oocytes. Braz J Med Biol Res 2004; 37(11): 1747-55. [DOI:10.1590/S0100-879X2004001100021]
4. Revelli A, Delle Piane L, Casano S, et al. Follicular Fluid Content and Oocyte Quality: from Single Biochemical Markers to Metabolomics. Reprod Biol Endocrinol 2009; 7: 40. [DOI:10.1186/1477-7827-7-40]
5. Levy G, Hill MJ, Ramirez C, et al. Serum Human Chorionic Gonadotropin Levels on the Day before Oocyte Retrieval do not Correlate with Oocyte Maturity. Fertil Steril 2013; 99(6): 1610-4. [DOI:10.1016/j.fertnstert.2012.12.053]
6. ÜÇkardeŞler ÖM, Kiliç S, Özakşit G, et al. Human Chorionic Gonadotropin Levels in Serum and Follicular Fluid are Correlated with Body Mass Index Rather than the Route of Administration of Purified hCG in ART Cycles. Turk J Med Sci 2009; 39(3): 389-96.
7. Zhang L, Wang H, Zhang R, et al. [Correlation of Follicular Fluid Human Chorionic Gonadotrophin Level with Oocyte Maturity and Early Embryonic Development]. Nan Fang Yi Ke Da Xue Xue Bao 2014; 34(2): 260-4.
8. Snoeck F, Sarrazin S, Wydooghe E, et al. Age and Anti-Mullerian Hormone Levels Predict the Success of in Vitro Maturation of Cat Oocytes. Reprod Domest Anim 2017; 52(Suppl 2): 98-102. [DOI:10.1111/rda.12827]
9. Kim JH, Lee JR, Chang HJ, et al. Anti-Mullerian Hormone Levels in the Follicular Fluid of the Preovulatory Follicle: A Predictor for Oocyte Fertilization and Quality of Embryo. J Korean Med Sci 2014; 29(9): 1266-70. [DOI:10.3346/jkms.2014.29.9.1266]
10. Simoes-Pereira J, Nunes J, Aguiar A, et al. Influence of Body Mass Index in AntiMullerian Hormone Levels in 951 NonPolycystic Ovarian Syndrome Women Followed at a Reproductive Medicine Unit. Endocrine 2018; 61(1): 144-8. [DOI:10.1007/s12020-018-1555-y]
11. Cupisiti S, Dittrich R, Muller A, et al. Correlation between Anti-Mullerian Hormone, Inhibin B, and Activin A in Follicular Fluid in IVF/ICSI Patints for Assessing the Maturation and Developmental Potential of Oocyte. Eur J Med Res 2007; 12(12): 604-8.
12. Takahashi C, Fujito A, Kazuka M, et al. Anti Mullerian Hormone Substance from Follicular Fluid is Positively Associated with Success in Oocyte Fertilization During in Vitro Fertilization. Fertil Steril 2008; 89(3): 586-91. [DOI:10.1016/j.fertnstert.2007.03.080]
13. Fadini R, Comi R, Mignini Renzini M, et al. Anti-Mullerian Hormone as a Predictive Marker for the Selection of Women for Oocyte in Vitro Maturation Treatment. J Assist Reprod Genet 2011; 28(6): 501-8. [DOI:10.1007/s10815-011-9589-6]
14. Zhang Y, Shao L, Xu Y, et al. Effect of AntiMullerian Hormone in Culture Medium on Quality of Mouse Oocytes Matured in Vitro. Plos One 2014; 9(6): e99393. [DOI:10.1371/journal.pone.0099393]
15. Carpintero NL, Suarez OA, Mangas CC, et al. Follicular Steroid Hormones as Markers of Oocyte Quality and Oocyte Development Potential. J Hum Reprod Sci 2014; 7(3): 187-93. [DOI:10.4103/0974-1208.142479]
16. Sarhan D, El Mazny A, Taha T, et al. Estradiol and Luteinizing Hormone Concentrations in the Follicular Aspirate During Ovum Pickup as Predictors of in Vitro Fertilization (IVF) Outcome. Mid East Fertil Soc J 2017; 22(1): 27-32. [DOI:10.1016/j.mefs.2016.09.005]
17. Salehnia M, Zavareh S. The Effects of Progesterone on Oocyte Maturation and Embryo Development. Int J Fertil Steril 2013; 7(2): 74-81.
18. Theofanakis C, Drakakis P, Besharat A, et al. Human Chorionic Gonadotropin: The Pregnancy Hormone and More. Int J Mol Sci 2017; 18(5): 1059. [DOI:10.3390/ijms18051059]
19. Rahmani E, Ahmadi SH, Motamed N, et al. Study of Association Between Ovarian Volume with the Number of Antral Follicles and Third Day of Menstruation FSH in Infertile Patients Referred to Omid Persian Gulf Infertility Clinic. Iran South Med J 2016; 19(4): 608-19. [DOI:10.18869/acadpub.ismj.19.4.608]
20. Luddi A, Capaldo A, Focarelli R, et al. Antioxidants Reduce Oxidative Stress in Follicular Fluid of Aged Women Undergoing IVF. Reprod Biol Endocrinol 2016; 14(1): 57. [DOI:10.1186/s12958-016-0184-7]
21. Kudsy M, Alhalabi M, Al-Quobaili F. Follicular Fluid Vascular Endothelial Growth Factor (VEGF) Could be a Predictor for Pregnancy Outcome in Normo-Responders and Polycystic Ovary Syndrome Women Undergoing IVF/ICSI Treatment Cycles. Mid East Fertil Soc J 2016; 21(1): 52-6. [DOI:10.1016/j.mefs.2015.11.001]
22. Tramisak Milakovic T, Panic Horvat L, Cavlovic K, et al. Follicular Fluid AntiMullerian Hormone: a Predictive Marker of Fertilization Capacity of MII Oocytes. Arch Gynecol Obstet 2015; 291(3): 681-7. [DOI:10.1007/s00404-014-3460-9]
23. Stojsin Carter A. Anti-Mullerian Hormone as a Marker of Oocyte Quantity, Developmental Potential, and Fetal Sex [dissertation]. Guelph Univ., 2016.
24. Lin D, Ran J, Zhu S, et al. Effect of GOLPH3 on Cumulus Granulosa Cell Apoptosis and ICSI Pregnancy Outcomes. Sci rep 2017; 7(1): 7863. [DOI:10.1038/s41598-017-08343-w]
25. Kedem-Dickman A, Maman E, Yung Y, et al. Anti-Mullerian Hormone is Highly Expressed and Secreted from Cumulus Granulosa Cells of Stimulated Preovulatory Immature and Atretic Oocytes. Reprod Biomed Online 2012; 24(5): 540-6. [DOI:10.1016/j.rbmo.2012.01.023]
26. Mehta BN, Chimote MN, Chimote NN, et al. Follicular-Fluid Anti-Mullerian Hormone (FF AMH) is a Plausible Biochemical Indicator of Functional Viability of Oocyte in Conventional in Vitro Fertilization (IVF) Cycles. J Hum Reprod Sci 2013; 6(2): 99-105. [DOI:10.4103/0974-1208.117168]
27. Weghofer A, Kim A, Barad DH, et al. Follicle Stimulating Hormone and Anti-Mullerian Hormone Per Oocyte in Predicting in Vitro Fertilization Pregnancy in High Responders: a Cohort Study. Plos One 2012; 7(4): e34290. [DOI:10.1371/journal.pone.0034290]
28. Revelli A, Canosa S, Bergandi L, et al. Oocyte Polarized Light Microscopy, Assay of Specific Follicular Fluid Metabolites, and Gene Expression in Cumulus Cells as Different Approaches to Predict Fertilization Efficiency after ICSI. Reprod Biol Endocrinol 2017; 15(1): 47. [DOI:10.1186/s12958-017-0265-2]
29. Cadoret V, Frapsauce C, Jarrier P, et al. Molecular Evidence that Follicle Development is Accelerated in Vitro Compared to in Vivo. Reproduction 2017; 153(5): 493-508. [DOI:10.1530/REP-16-0627]
30. Costa LO, Mendes MC, Ferriani RA, et al. Estradiol and Testosterone Concentrations in Follicular Fluid as Criteria to Discriminate between Mature and Immature Oocytes. Braz J Med Biol Res 2004; 37(11): 1747-55. [DOI:10.1590/S0100-879X2004001100021]
31. Revelli A, Delle Piane L, Casano S, et al. Follicular Fluid Content and Oocyte Quality: from Single Biochemical Markers to Metabolomics. Reprod Biol Endocrinol 2009; 7: 40. [DOI:10.1186/1477-7827-7-40]
32. Levy G, Hill MJ, Ramirez C, et al. Serum Human Chorionic Gonadotropin Levels on the Day before Oocyte Retrieval do not Correlate with Oocyte Maturity. Fertil Steril 2013; 99(6): 1610-4. [DOI:10.1016/j.fertnstert.2012.12.053]
33. ÜÇkardeŞler ÖM, Kiliç S, Özakşit G, et al. Human Chorionic Gonadotropin Levels in Serum and Follicular Fluid are Correlated with Body Mass Index Rather than the Route of Administration of Purified hCG in ART Cycles. Turk J Med Sci 2009; 39(3): 389-96.
34. Zhang L, Wang H, Zhang R, et al. [Correlation of Follicular Fluid Human Chorionic Gonadotrophin Level with Oocyte Maturity and Early Embryonic Development]. Nan Fang Yi Ke Da Xue Xue Bao 2014; 34(2): 260-4.
35. Snoeck F, Sarrazin S, Wydooghe E, et al. Age and Anti-Mullerian Hormone Levels Predict the Success of in Vitro Maturation of Cat Oocytes. Reprod Domest Anim 2017; 52(Suppl 2): 98-102. [DOI:10.1111/rda.12827]
36. Kim JH, Lee JR, Chang HJ, et al. Anti-Mullerian Hormone Levels in the Follicular Fluid of the Preovulatory Follicle: A Predictor for Oocyte Fertilization and Quality of Embryo. J Korean Med Sci 2014; 29(9): 1266-70. [DOI:10.3346/jkms.2014.29.9.1266]
37. Simoes-Pereira J, Nunes J, Aguiar A, et al. Influence of Body Mass Index in AntiMullerian Hormone Levels in 951 NonPolycystic Ovarian Syndrome Women Followed at a Reproductive Medicine Unit. Endocrine 2018; 61(1): 144-8. [DOI:10.1007/s12020-018-1555-y]
38. Cupisiti S, Dittrich R, Muller A, et al. Correlation between Anti-Mullerian Hormone, Inhibin B, and Activin A in Follicular Fluid in IVF/ICSI Patints for Assessing the Maturation and Developmental Potential of Oocyte. Eur J Med Res 2007; 12(12): 604-8.
39. Takahashi C, Fujito A, Kazuka M, et al. Anti Mullerian Hormone Substance from Follicular Fluid is Positively Associated with Success in Oocyte Fertilization During in Vitro Fertilization. Fertil Steril 2008; 89(3): 586-91. [DOI:10.1016/j.fertnstert.2007.03.080]
40. Fadini R, Comi R, Mignini Renzini M, et al. Anti-Mullerian Hormone as a Predictive Marker for the Selection of Women for Oocyte in Vitro Maturation Treatment. J Assist Reprod Genet 2011; 28(6): 501-8. [DOI:10.1007/s10815-011-9589-6]
41. Zhang Y, Shao L, Xu Y, et al. Effect of AntiMullerian Hormone in Culture Medium on Quality of Mouse Oocytes Matured in Vitro. Plos One 2014; 9(6): e99393. [DOI:10.1371/journal.pone.0099393]
42. Carpintero NL, Suarez OA, Mangas CC, et al. Follicular Steroid Hormones as Markers of Oocyte Quality and Oocyte Development Potential. J Hum Reprod Sci 2014; 7(3): 187-93. [DOI:10.4103/0974-1208.142479]
43. Sarhan D, El Mazny A, Taha T, et al. Estradiol and Luteinizing Hormone Concentrations in the Follicular Aspirate During Ovum Pickup as Predictors of in Vitro Fertilization (IVF) Outcome. Mid East Fertil Soc J 2017; 22(1): 27-32. [DOI:10.1016/j.mefs.2016.09.005]
44. Salehnia M, Zavareh S. The Effects of Progesterone on Oocyte Maturation and Embryo Development. Int J Fertil Steril 2013; 7(2): 74-81.
45. Theofanakis C, Drakakis P, Besharat A, et al. Human Chorionic Gonadotropin: The Pregnancy Hormone and More. Int J Mol Sci 2017; 18(5): 1059. [DOI:10.3390/ijms18051059]
46. Rahmani E, Ahmadi SH, Motamed N, et al. Study of Association Between Ovarian Volume with the Number of Antral Follicles and Third Day of Menstruation FSH in Infertile Patients Referred to Omid Persian Gulf Infertility Clinic. Iran South Med J 2016; 19(4): 608-19. [DOI:10.18869/acadpub.ismj.19.4.608]
47. Luddi A, Capaldo A, Focarelli R, et al. Antioxidants Reduce Oxidative Stress in Follicular Fluid of Aged Women Undergoing IVF. Reprod Biol Endocrinol 2016; 14(1): 57. [DOI:10.1186/s12958-016-0184-7]
48. Kudsy M, Alhalabi M, Al-Quobaili F. Follicular Fluid Vascular Endothelial Growth Factor (VEGF) Could be a Predictor for Pregnancy Outcome in Normo-Responders and Polycystic Ovary Syndrome Women Undergoing IVF/ICSI Treatment Cycles. Mid East Fertil Soc J 2016; 21(1): 52-6. [DOI:10.1016/j.mefs.2015.11.001]
49. Tramisak Milakovic T, Panic Horvat L, Cavlovic K, et al. Follicular Fluid AntiMullerian Hormone: a Predictive Marker of Fertilization Capacity of MII Oocytes. Arch Gynecol Obstet 2015; 291(3): 681-7. [DOI:10.1007/s00404-014-3460-9]
50. Stojsin Carter A. Anti-Mullerian Hormone as a Marker of Oocyte Quantity, Developmental Potential, and Fetal Sex [dissertation]. Guelph Univ., 2016.
51. Lin D, Ran J, Zhu S, et al. Effect of GOLPH3 on Cumulus Granulosa Cell Apoptosis and ICSI Pregnancy Outcomes. Sci rep 2017; 7(1): 7863. [DOI:10.1038/s41598-017-08343-w]
52. Kedem-Dickman A, Maman E, Yung Y, et al. Anti-Mullerian Hormone is Highly Expressed and Secreted from Cumulus Granulosa Cells of Stimulated Preovulatory Immature and Atretic Oocytes. Reprod Biomed Online 2012; 24(5): 540-6. [DOI:10.1016/j.rbmo.2012.01.023]
53. Mehta BN, Chimote MN, Chimote NN, et al. Follicular-Fluid Anti-Mullerian Hormone (FF AMH) is a Plausible Biochemical Indicator of Functional Viability of Oocyte in Conventional in Vitro Fertilization (IVF) Cycles. J Hum Reprod Sci 2013; 6(2): 99-105. [DOI:10.4103/0974-1208.117168]
54. Weghofer A, Kim A, Barad DH, et al. Follicle Stimulating Hormone and Anti-Mullerian Hormone Per Oocyte in Predicting in Vitro Fertilization Pregnancy in High Responders: a Cohort Study. Plos One 2012; 7(4): e34290. [DOI:10.1371/journal.pone.0034290]
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