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:: Volume 23, Issue 4 (Iranian South Medical Journal 2020) ::
Iran South Med J 2020, 23(4): 292-301 Back to browse issues page
Accuracy of Indirect ELISA Prepared from Recombinant Bp26 Gene of Brucella melitensis in Diagnosis of Human Brucellosis
Seyed Davood Hoseini1 , Ehsanollah Ghaznavirad2, Ali Asghar Farazi3
1- Department of Molecular Biotechnology, Razi Vaccine and Serum Research Instutute, Agricultural, Research Education and Extension Organization (AREEO), Arak Branch, Arak
2- Department of Clinical Microbiology, School of Medicine, Arak University of Medical Sciences, Arak, Iran
3- Department of Infectious Diseases, School of Medicine, Arak University of Medical Sciences, Arak, Iran
Abstract:   (676 Views)
Background: Considering the prevalence of brucellosis in Iran, it is necessary to choose a specific and sensitive laboratory method to diagnose it in a rapid and timely manner. The aim of this study was to assess the accuracy of indirect enzyme-linked immunosorbent assay (ELISA) for detecting brucellosis in humans in order to have an appropriate alternative to conventional tests such as Wright, 2ME, and commercial ELISA kits.
Materials and Methods: In this study, the recombinant protein produced from the gene (omp28) bp26 Brucella melitensis was used as an antigen for coating microplate wells. A total of 124 serum samples of normal healthy individuals (n=62) and patients with acute brucellosis (n=62) approved by STA and 2 ME tests were entered into the study. The data were analyzed in SPSS (ver.18).
Results: The mean age was 39.8±13.5 years in the patient group and 36.1±12.7 years in the healthy group. Furthermore, 66.1% of the patients were male and 62.9% lived in rural regions, while these figures were respectively 71% and 45.2% in the healthy group. The sensitivity of 92% and specificity of 87% and a positive predictive value of 88% and a negative predictive value of 92% and an accuracy of 90% were determined for ELISA kit used in this study.
Conclusion: The ELISA diagnostic kit reacted to most of the positive human sera. However, this kit needs to be further evaluated with a larger sample size of clinical specimens from different regions and with
various clinical forms of human brucellosis.
Keywords: Brucellosis, Recombinant proteins, ELISA, bp26 gene
Full-Text [PDF 952 kb]   (160 Downloads)    
Type of Study: Original | Subject: Microbiology and Immunology
Received: 2019/06/13 | Accepted: 2020/05/30 | Published: 2020/09/30
1. De Figueiredo P, Ficht TA, Rice-Ficht A, et al. Pathogenesis And Immunobiology Of Brucellosis: Review Of Brucella-Host Interactions. Am J Pathol 2015; 185(6): 1505-17. [DOI:10.1016/j.ajpath.2015.03.003]
2. Farazi AA, Sofian M, Ghazisaeedi M. Laboratory features of patients with Brucellosis and its association with titer of Wright agglutination test. Iran South Med J 2014; 17(5): 860-6. (Persian)
3. Avijgan M, Rostamnezhad M, JahanbaniArdakani H. Clinical And Serological Approach To Patients With Brucellosis: A Common Diagnostic Dilemma And A Worldwide Perspective. Microb Pathog 2019; 129: 125-30. [DOI:10.1016/j.micpath.2019.02.011]
4. Abernethy DA, Moscard-Costello J, Dickson E, et al. Epidemiology And Management Of A Bovine Brucellosis Cluster In Northern Ireland. Prev Vet Med 2011; 98(4): 223-9. [DOI:10.1016/j.prevetmed.2010.11.002]
5. Amjadi O, Rafiei A, Mardani M, et al. A Review Of The Immunopathogenesis Of Brucellosis. Infect Dis (Lond) 2019; 51(5): 321-33. [DOI:10.1080/23744235.2019.1568545]
6. Gopalakrishnan A, Dimri U, Saminathan M, et al. Virulance Factors Intracellular Survuvability And Mechanism Of Evasion From Host Immune Response By Brucella: An Overview. Anim Plant Sci 2016; 26(6): 1542-55.
7. Ica T, Aydin F, Gümüşsoy KS, et al. Conventional And Molecular Biotyping Of Brucella Strains Isolated From Cattle, Sheep And Human. J Vet Faculty Ankara Univ 2012; 59(4): 259-64. [DOI:10.1501/Vetfak_0000002536]
8. Tomaso H, Kattar M, Eickhoff M, et al. Comparlison Of Commercial DNA Prepration Kits For The Detection Of Brucellae In Tissue Using Quantitative Real-Time PCR. BMC Infect Dis 2010; 10: 100. [DOI:10.1186/1471-2334-10-100]
9. Gall D, Nielsen K. Serological Diagnosis Of Bovine Brucellosis: A Review Of Test Performance And Cost Comparison. Rev Sci Tech 2004; 23(3): 989-1002. [DOI:10.20506/rst.23.3.1545]
10. Cassataro J, Velikovsky CA, Bruno L, et al. Improved Immunogenicity Of A Vaccination Regimen Combining A DNA Vaccine Encoding Brucella Melitensis Outer Membrane Protein 31 (Omp31) And Recombinant Omp31 Boosting. Clin Vaccine Immunol 2007; 14(7): 869-74. [DOI:10.1128/CVI.00472-06]
11. Cloeckaert A, Vizcaíno N, Paquet JY, et al. Major Outer Membrane Proteins Of Brucella spp: Past, Present And Future. Vet Microbiol 2002; 90(1-4): 229-47. [DOI:10.1016/S0378-1135(02)00211-0]
12. Azizpour M, Hosseini D, Akbary N, et al. Amplification, Cloning, And Expression Of Brucella Melitensis bp26 Gene Isolated From Markazi Province In Order To Produce BP26 Recombinant Protein. J Arak Uni Med Sci 2013; 16(3): 62-70. (Persian)
13. Chaudhuri P, Prasad R, Kumar V. Recombinant OMP28 Antigen Based Indirect ELISA For Serodiagnosis Of Bovine Brucellosis. Mol Cell Probes 2010; 24(3): 142-5. [DOI:10.1016/j.mcp.2009.12.002]
14. Cassataro J, Pasquevich K, Bruno L, et al. Antibody Reactivity To Omp31 From Brucella Melitensis In Human And Animal Infections By Smooth And Rough Brucellae. Clin Diagn Lab Immunol 2004; 11(1): 111-4. [DOI:10.1128/CDLI.11.1.111-114.2004]
15. Farazi A, Hosseini SD. Diagnostic Validity Of The Conventional Brucellosis Serological Tests In. J Arak Uni Med Sci 2012; 14(7): 71-7. (Persian)
16. Najafi N, Davoodi L, Fazli M, et al. Diagnostic Value Of Elisa Versus Wright In Human Brucellosis With Positive PCR. J Mazandaran Univ Med Sci 2014; 23(1): 21-8. (Persian)
17. Samavati N, Mirjalili A, Boutorabi M. An Indirect Enzyme-Linked Immunosorbent Assay To Detect Antibodies Against Brucellosis In Cattle Or Humans. J Isfahan Med Sch 2012; 30(205): 1403-14. (Persian)
18. Shapoury R, Imani Fooladi AA, Rahnama M, et al. Designing And Validation Of Indirect And Competitive ELISA For Diagnosis Of Brucellosis In Human. J Mil Med 2009; 11(1): 51-6. (Persian)
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Hoseini S D, Ghaznavirad E, Farazi A A. Accuracy of Indirect ELISA Prepared from Recombinant Bp26 Gene of Brucella melitensis in Diagnosis of Human Brucellosis. Iran South Med J. 2020; 23 (4) :292-301
URL: http://ismj.bpums.ac.ir/article-1-1328-en.html

Volume 23, Issue 4 (Iranian South Medical Journal 2020) Back to browse issues page
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