دوره 25، شماره 4 - ( دو ماهنامه طب جنوب 1401 )                   جلد 25 شماره 4 صفحات 325-297 | برگشت به فهرست نسخه ها


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Dehghani H, Rashedinia M, Mohebbi G H, Vazirizadeh A, Maryamabadi A, Barmak A R. The in vitro and in silico Anticholinesterase Ac-tivities of Brittle Star (Ophiocoma erinaceus) crude venoms from the Persian Gulf-Bushehr. Iran South Med J 2022; 25 (4) :297-325
URL: http://ismj.bpums.ac.ir/article-1-1639-fa.html
دهقانی حمیده، راشدی‌نیا مرضیه، محبی غلامحسین، وزیری‌زاده امیر، مریم‌آبادی عمار، برمک علیرضا. مطالعه فعالیت‌های آنتی‌کولین استرازی در شرایط in vitro و in silico زهر خام ستاره شکننده اوفیوکوما اریناسئوس خلیج‌فارس- بوشهر. طب جنوب 1401; 25 (4) :325-297

URL: http://ismj.bpums.ac.ir/article-1-1639-fa.html


1- گروه سم‌شناسی و فارماکولوژی، دانشکده داروسازی، دانشگاه علوم پزشکی شیراز، شیراز- ایران
2- مرکز تحقیقات زیست فناوری دریایی‌ خلیج‌فارس، پژوهشکده علوم زیست پزشکی خلیج‌‌فارس، دانشگاه علوم پزشکی بوشهر، بوشهر، ایران ، Mohebbihsn@yahoo.com
3- گروه زیست‌شناسی دریا، مرکز مطالعات و پژوهش‌های خلیج‌فارس، دانشگاه خلیج‌فارس، بوشهر، ایران
4- مرکز تحقیقات زیست فناوری دریایی‌ خلیج‌فارس، پژوهشکده علوم زیست پزشکی خلیج‌‌فارس، دانشگاه علوم پزشکی بوشهر، بوشهر، ایران
چکیده:   (208 مشاهده)
زمینه: ستاره‌های شکننده، علاوه بر دفاع فیزیکی، قادر به تولید توکسین‌های شناخته شده‌ای می‌باشند که مسئول فعالیت­های بیولوژیکی مختلف آن‌ها است. با توجه به فراوانی این زیست‌مندان دریایی در آب‌های ساحلی خلیج‌فارس و با علم به اثرات بیولوژیک متعدد آن‌ها، این مطالعه با هدف شناسایی متابولیت‌های ثانویه و ارزیابی فعالیت­های in vitro و in silico آنتی‌کولین استرازی زهر خام اکینودرم اوفیوکوما اریناسئوس خلیج‌فارس انجام گردید.
مواد و روش‌ها: در این مطالعه، پس از لیوفیلیزاسیون نمونه ستاره شکننده، آزمون­های LD50، فعالیت­های مهارکنندگی کولین­استرازی، شناسایی متابولیت­های ثانویه و ارزیابی درون­رایانه‌ای آن‌ها به‌ترتیب با روش‌های اسپیرمن-کاربر، اسپکتروسکوپی المن، کروماتوگرافی گازی- طیف‌سنجی جرمی (GC-MS) و روش محاسباتی داکینگ انجام گردیدند.
یافته‌ها: بر اساس نتایج، میزان LD50 نمونه 0.13±6.04 میلی‌گرم بر کیلوگرم بود. مقادیر IC50 مربوط به آنزیم استیل کولین‌استراز و بوتیریل کولین‌استراز آن‌ها به‌ترتیب 0.055±37/925 و نیز 0/02±5/388 میکروگرم بر میلی‌لیتر در مقایسه با استاندارد گالانتامین به دست آمدند. آنالیز GC-MS نمونه تعداد 25 ترکیب شیمیایی زیست­فعال با ساختارهای مختلف نظیر آلکالوئیدها، ترپن‌ها و استروئیدها را نشان داد. نتایج محاسباتی ترکیبات نیز، نتایج تجربی را تأئید نمودند. از این میان، ترکیب آلکالوئیدی BS4 دارای بیشترین تمایل برای هر دو آنزیم بود.
نتیجه‌گیری: از نظر قدرت سمیت، نمونه زهر خام ستاره شکننده در گروه "خیلی سمی" قرار گرفت. آنالیز کروماتوگرافی گازی زهر خام، متابولیت‌های زیست­فعال ثانویه متعددی با ساختارهای شیمیایی متفاوتی را نشان داد. نتایج تجربی و محاسباتی، روی فعالیت آنزیم­های کولین‌استرازی نمونه نشان داد که زهر، به‌عنوان مهارکننده قابل ملاحظه آنزیم­ها عمل می­نماید. مطالعات بیشتری برای تعیین اینکه آیا ترکیب BS4 می‌تواند کاندیدای درمان بیماری آلزایمر باشد، مورد نیاز است.
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نوع مطالعه: پژوهشي | موضوع مقاله: بیوشیمی
دریافت: 1401/2/25 | پذیرش: 1401/6/28 | انتشار: 1401/9/26

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